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The map label for this gene is paaG [C]

Identifier: 169630362

GI number: 169630362

Start: 3319730

End: 3320497

Strand: Direct

Name: paaG [C]

Synonym: MAB_3281

Alternate gene names: 169630362

Gene position: 3319730-3320497 (Clockwise)

Preceding gene: 169630361

Following gene: 169630366

Centisome position: 65.51

GC content: 68.62

Gene sequence:

>768_bases
GTGGCATCTGATTACATCGCCGTCCACAACGGCGTACTGCATATCACCATCGCCACCGCGGCGGCCGGTACCTCGCTGGA
CTTCACCGGCGTCGACGCCGCGGTCCAGGTGCTGCGTGATCTGCCCGCCGAAGTAGGCGCGATCCTGCTGACCAGCAGTG
GGCCCAACTTCTGCGCTGGCGGCAATGTCCGTGACTTCGCCTCTGCCGAGGATAGGTCGGCCAAGCTCCACGACCTGGCC
GGGCGGCTCCACGCGTTCGTGCGTGCGGTGGACGCCGCCGATCGCCCGGTAGTGGCCTCGGTCCACGGATGGGCGGCCGG
CGGCGGACTCAGTCTGGTGCTGCTCGCCGATATCGCCATCGGTGGACAGTCAACCAAGCTGCGCGCCGCCTACCCGGGCA
TCGGGTACTCCTCCGATGGCGGCATGTCCTGGGCGCTGCCCCGCGTGGTCGGCAAGGCCCGTGCCGCGGACATTCTGCTG
ACGGATCGGGTTGTCGAAGCCTCCGAGGCACTGTCCATCGGGCTGTTGAGCCGGGTGGTGGCCGACGACGAGGTTCAGGC
GACTGCGGTGGCCACCGCCGAGCAGCTGGCCGCCGGTCCGCGCGAAGCGCAGAACCGTATTCGTCGTCTGCTGCGCCTGA
GCGCCACCAACACGCTCGATGCGCAACTCGATCTCGAGCAGACCAGCATCGCCGAGGCGTCGGTCAGCCCGACCGGTATC
GAGGGCGTCGACGCCTTCGTCGGCAAACGCAAGCCCGTCTGGCCCTGA

Upstream 100 bases:

>100_bases
CACCTCTCCCGGTGGCAACAAGCAGCGCGTCAACGCCTGCACCTCCTGCATCAAGGCCGGCAAGGTCGTCCGCGGCGCCT
GATCGCGTCCGGAACCTGCC

Downstream 100 bases:

>100_bases
GCTAGCCGAGTTCCGCGGTCTTCGCGCCAACGCTCATCCCAGTTCCGCGGTCTTCGCGCCAACGCTCATCCCAGTTCCGC
CGTCTTCGCGCCAACGCTCA

Product: putative enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 4-CBA-CoA dehalogenase; 4-CBCoA dehalogenase; 4-chlorobenzoyl-CoA dehalogenase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MASDYIAVHNGVLHITIATAAAGTSLDFTGVDAAVQVLRDLPAEVGAILLTSSGPNFCAGGNVRDFASAEDRSAKLHDLA
GRLHAFVRAVDAADRPVVASVHGWAAGGGLSLVLLADIAIGGQSTKLRAAYPGIGYSSDGGMSWALPRVVGKARAADILL
TDRVVEASEALSIGLLSRVVADDEVQATAVATAEQLAAGPREAQNRIRRLLRLSATNTLDAQLDLEQTSIAEASVSPTGI
EGVDAFVGKRKPVWP

Sequences:

>Translated_255_residues
MASDYIAVHNGVLHITIATAAAGTSLDFTGVDAAVQVLRDLPAEVGAILLTSSGPNFCAGGNVRDFASAEDRSAKLHDLA
GRLHAFVRAVDAADRPVVASVHGWAAGGGLSLVLLADIAIGGQSTKLRAAYPGIGYSSDGGMSWALPRVVGKARAADILL
TDRVVEASEALSIGLLSRVVADDEVQATAVATAEQLAAGPREAQNRIRRLLRLSATNTLDAQLDLEQTSIAEASVSPTGI
EGVDAFVGKRKPVWP
>Mature_254_residues
ASDYIAVHNGVLHITIATAAAGTSLDFTGVDAAVQVLRDLPAEVGAILLTSSGPNFCAGGNVRDFASAEDRSAKLHDLAG
RLHAFVRAVDAADRPVVASVHGWAAGGGLSLVLLADIAIGGQSTKLRAAYPGIGYSSDGGMSWALPRVVGKARAADILLT
DRVVEASEALSIGLLSRVVADDEVQATAVATAEQLAAGPREAQNRIRRLLRLSATNTLDAQLDLEQTSIAEASVSPTGIE
GVDAFVGKRKPVWP

Specific function: Dehalogenates 4-chlorobenzoyl-CoA, 4-iodobenzoyl-CoA and 4-bromobenzoyl-CoA, but not 4-fluorobenzoyl-CoA. Inactive towards crotonyl-CoA, alpha-methylcrotonyl-CoA and beta-methylcrotonyl- CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=216, Percent_Identity=28.7037037037037, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI194097323, Length=224, Percent_Identity=27.2321428571429, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757968, Length=211, Percent_Identity=26.5402843601896, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI49355787, Length=211, Percent_Identity=26.5402843601896, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI25453481, Length=211, Percent_Identity=26.0663507109005, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538573, Length=211, Percent_Identity=26.0663507109005, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4757966, Length=204, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI51538581, Length=204, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=23.9495798319328, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=23.9495798319328, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI70995211, Length=165, Percent_Identity=31.5151515151515, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=23.9495798319328, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787660, Length=221, Percent_Identity=36.6515837104072, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=29.3893129770992, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=26.8199233716475, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI221142681, Length=221, Percent_Identity=28.0542986425339, Blast_Score=74, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=215, Percent_Identity=30.2325581395349, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=228, Percent_Identity=30.2631578947368, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=227, Percent_Identity=25.1101321585903, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=28.0788177339901, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=213, Percent_Identity=29.5774647887324, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=27.5555555555556, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=29.2307692307692, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI20129971, Length=206, Percent_Identity=27.1844660194175, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653477, Length=206, Percent_Identity=27.1844660194175, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24654903, Length=168, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920382, Length=190, Percent_Identity=28.9473684210526, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =3.8.1.7 [H]

Molecular weight: Translated: 26282; Mature: 26151

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDYIAVHNGVLHITIATAAAGTSLDFTGVDAAVQVLRDLPAEVGAILLTSSGPNFCAG
CCCCEEEEECCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHCEEEEECCCCCCCCC
GNVRDFASAEDRSAKLHDLAGRLHAFVRAVDAADRPVVASVHGWAAGGGLSLVLLADIAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEHHHC
GGQSTKLRAAYPGIGYSSDGGMSWALPRVVGKARAADILLTDRVVEASEALSIGLLSRVV
CCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
ADDEVQATAVATAEQLAAGPREAQNRIRRLLRLSATNTLDAQLDLEQTSIAEASVSPTGI
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHCCCCCCCC
EGVDAFVGKRKPVWP
CHHHHHHCCCCCCCC
>Mature Secondary Structure 
ASDYIAVHNGVLHITIATAAAGTSLDFTGVDAAVQVLRDLPAEVGAILLTSSGPNFCAG
CCCEEEEECCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHCEEEEECCCCCCCCC
GNVRDFASAEDRSAKLHDLAGRLHAFVRAVDAADRPVVASVHGWAAGGGLSLVLLADIAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEHHHC
GGQSTKLRAAYPGIGYSSDGGMSWALPRVVGKARAADILLTDRVVEASEALSIGLLSRVV
CCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
ADDEVQATAVATAEQLAAGPREAQNRIRRLLRLSATNTLDAQLDLEQTSIAEASVSPTGI
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHCCCCCCCC
EGVDAFVGKRKPVWP
CHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1351742; 1610806 [H]