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The map label for this gene is trmD [H]

Identifier: 169630307

GI number: 169630307

Start: 3262605

End: 3263333

Strand: Reverse

Name: trmD [H]

Synonym: MAB_3226c

Alternate gene names: 169630307

Gene position: 3263333-3262605 (Counterclockwise)

Preceding gene: 169630308

Following gene: 169630305

Centisome position: 64.4

GC content: 63.24

Gene sequence:

>729_bases
ATGAAAATAGACGTCGTCACGATCTTCCCGGAATATCTGCAACCCGTGCGGCAGTCCTTGCCGGGCAAGGCTATTGACGC
TGGTTTGGTCGATGTCGCGGTGCATGATCTGCGGCGCTGGACGCACGACGTGCACAAGTCCGTCGATGACTCGCCATACG
GTGGTGGGCCCGGCATGGTGATGAAGCCGACGGTCTGGGGCGATGCGCTGGATGAGATCTGCACATCTGAAACGCTTTTG
GTGGTGCCGACACCCGCTGGCTACCCGTTCACCCAGGAGACCGCCTGGCAGTGGTCCACCGAAGATCATCTGGTGATCGC
ATGTGGGCGGTACGAGGGAATCGACCAGCGCGTCGCCGACGACGCCGCGACACGGATGCGGGTGCGCGAGGTGTCGATCG
GTGACTACGTACTCAACGGCGGCGAGGCCGCTGCTCTGGTGATCATCGAAGCGGTGTTGCGTCTGGTTCCCGGTGTGCTG
GGCAACGCGCTTTCCGCACAGGAAGATTCACACTCCGAGGGAATGGCCAGCCTCTTGGAGGGGCCGTCGTACACCAGACC
GCCGAGCTGGCGGGGGATGGATGTGCCACCGGTGCTGCTCTCGGGAGATCACGCCAAGATCGCGGCCTGGCGGGCCGAAC
AGTCGCGTCAGCGCACTATTGAGCGGCGCCCAGATCTACTCGGTTTCGATTCGCCCACCGGGGAACATGGTGGCGACGGC
CTGAGTTAG

Upstream 100 bases:

>100_bases
CCGTTCGTCTCCGCGATCGTCACGTCGATCTCTTTGGCGGACAAGGTTATCGAGATTGACCCCCCGGAAGGGCTGCTGGA
TCTGTCGTGAGAATCCCGTC

Downstream 100 bases:

>100_bases
CGTGTTGCGCGCCGTCACATCGTTGGCGGTCTGCAGTGACGACACCACCACGATGAATCGCCGGTCCGGGCCCACGACGC
CCGTCGAAAGGTGCATCCAC

Product: tRNA (guanine-N(1)-)-methyltransferase (TrmD)

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKIDVVTIFPEYLQPVRQSLPGKAIDAGLVDVAVHDLRRWTHDVHKSVDDSPYGGGPGMVMKPTVWGDALDEICTSETLL
VVPTPAGYPFTQETAWQWSTEDHLVIACGRYEGIDQRVADDAATRMRVREVSIGDYVLNGGEAAALVIIEAVLRLVPGVL
GNALSAQEDSHSEGMASLLEGPSYTRPPSWRGMDVPPVLLSGDHAKIAAWRAEQSRQRTIERRPDLLGFDSPTGEHGGDG
LS

Sequences:

>Translated_242_residues
MKIDVVTIFPEYLQPVRQSLPGKAIDAGLVDVAVHDLRRWTHDVHKSVDDSPYGGGPGMVMKPTVWGDALDEICTSETLL
VVPTPAGYPFTQETAWQWSTEDHLVIACGRYEGIDQRVADDAATRMRVREVSIGDYVLNGGEAAALVIIEAVLRLVPGVL
GNALSAQEDSHSEGMASLLEGPSYTRPPSWRGMDVPPVLLSGDHAKIAAWRAEQSRQRTIERRPDLLGFDSPTGEHGGDG
LS
>Mature_242_residues
MKIDVVTIFPEYLQPVRQSLPGKAIDAGLVDVAVHDLRRWTHDVHKSVDDSPYGGGPGMVMKPTVWGDALDEICTSETLL
VVPTPAGYPFTQETAWQWSTEDHLVIACGRYEGIDQRVADDAATRMRVREVSIGDYVLNGGEAAALVIIEAVLRLVPGVL
GNALSAQEDSHSEGMASLLEGPSYTRPPSWRGMDVPPVLLSGDHAKIAAWRAEQSRQRTIERRPDLLGFDSPTGEHGGDG
LS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=231, Percent_Identity=43.7229437229437, Blast_Score=177, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 26263; Mature: 26263

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDVVTIFPEYLQPVRQSLPGKAIDAGLVDVAVHDLRRWTHDVHKSVDDSPYGGGPGMV
CEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
MKPTVWGDALDEICTSETLLVVPTPAGYPFTQETAWQWSTEDHLVIACGRYEGIDQRVAD
ECCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHH
DAATRMRVREVSIGDYVLNGGEAAALVIIEAVLRLVPGVLGNALSAQEDSHSEGMASLLE
HHHHHHHHHEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GPSYTRPPSWRGMDVPPVLLSGDHAKIAAWRAEQSRQRTIERRPDLLGFDSPTGEHGGDG
CCCCCCCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LS
CC
>Mature Secondary Structure
MKIDVVTIFPEYLQPVRQSLPGKAIDAGLVDVAVHDLRRWTHDVHKSVDDSPYGGGPGMV
CEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
MKPTVWGDALDEICTSETLLVVPTPAGYPFTQETAWQWSTEDHLVIACGRYEGIDQRVAD
ECCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHH
DAATRMRVREVSIGDYVLNGGEAAALVIIEAVLRLVPGVLGNALSAQEDSHSEGMASLLE
HHHHHHHHHEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GPSYTRPPSWRGMDVPPVLLSGDHAKIAAWRAEQSRQRTIERRPDLLGFDSPTGEHGGDG
CCCCCCCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]