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The map label for this gene is bar [H]

Identifier: 169630122

GI number: 169630122

Start: 3090454

End: 3090987

Strand: Reverse

Name: bar [H]

Synonym: MAB_3039c

Alternate gene names: 169630122

Gene position: 3090987-3090454 (Counterclockwise)

Preceding gene: 169630123

Following gene: 169630120

Centisome position: 61.0

GC content: 65.36

Gene sequence:

>534_bases
ATGCACATTCGTGACGCGACGGCCGCCGATGCGGCCGCGTGTGCGGCAATCTATGCGCCGTATGTGACCGATACGGCCAT
CTCGTTTGAGGAGGTGCCGCCGTCGGATGCTGTTCTTGCCGAACGCATCCAGACGGCGGCCCACCGACATGCCTGGCTCG
TCGCCGAGGATGGCGGGGAAATTCTCGGCTACGCGTATGCGGCGCCGTGGAAGTCGCGCACTGCCTACCAATGGGCCTGT
GAGGTCAGTGTCTATGTCGACGGCGCGCGCAGGCAGCGCGGCACCGGACGCCAGTTGTACACAGCGTTACTCGGCCGGCT
ACGGGAGCTGGGTTATCGCACCGTGCTCGCGGTTGTCGTCACACCCAATGCGGCGAGTGAAAAGCTGCACCGGACAATGG
GATTCGAGCAGGTCGCGCTGTACCGGCGGATTGGATACAAGCTGGGCAGCTGGCATGACGTCACGCATTTTCAGCTGTTC
TTGACCTCCGCCGGCGATTCCCGCGCGCCGGGGCCGGCGCCCGGGGAGGCCTAG

Upstream 100 bases:

>100_bases
CCCGGTGGCCCGGGCATACATGGACTCCCGTATCCAGACGATCTACGGCGGCACTACCGAGATCATGAAGGAGATCATCG
GGCGTTCGCTCTAGTCGCCC

Downstream 100 bases:

>100_bases
ACCTTCCGGATAACGGTGACGACCTTGCCCAGAATGGCGGCGTCGTTGCCGGGAATCGGCTCGAAGAGTGGATTGTGCGG
CATCAGCCAGACCTGGCCCG

Product: phosphinothricin N-acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MHIRDATAADAAACAAIYAPYVTDTAISFEEVPPSDAVLAERIQTAAHRHAWLVAEDGGEILGYAYAAPWKSRTAYQWAC
EVSVYVDGARRQRGTGRQLYTALLGRLRELGYRTVLAVVVTPNAASEKLHRTMGFEQVALYRRIGYKLGSWHDVTHFQLF
LTSAGDSRAPGPAPGEA

Sequences:

>Translated_177_residues
MHIRDATAADAAACAAIYAPYVTDTAISFEEVPPSDAVLAERIQTAAHRHAWLVAEDGGEILGYAYAAPWKSRTAYQWAC
EVSVYVDGARRQRGTGRQLYTALLGRLRELGYRTVLAVVVTPNAASEKLHRTMGFEQVALYRRIGYKLGSWHDVTHFQLF
LTSAGDSRAPGPAPGEA
>Mature_177_residues
MHIRDATAADAAACAAIYAPYVTDTAISFEEVPPSDAVLAERIQTAAHRHAWLVAEDGGEILGYAYAAPWKSRTAYQWAC
EVSVYVDGARRQRGTGRQLYTALLGRLRELGYRTVLAVVVTPNAASEKLHRTMGFEQVALYRRIGYKLGSWHDVTHFQLF
LTSAGDSRAPGPAPGEA

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=182, Percent_Identity=32.967032967033, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 19338; Mature: 19338

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIRDATAADAAACAAIYAPYVTDTAISFEEVPPSDAVLAERIQTAAHRHAWLVAEDGGE
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCC
ILGYAYAAPWKSRTAYQWACEVSVYVDGARRQRGTGRQLYTALLGRLRELGYRTVLAVVV
EEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEE
TPNAASEKLHRTMGFEQVALYRRIGYKLGSWHDVTHFQLFLTSAGDSRAPGPAPGEA
CCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MHIRDATAADAAACAAIYAPYVTDTAISFEEVPPSDAVLAERIQTAAHRHAWLVAEDGGE
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCC
ILGYAYAAPWKSRTAYQWACEVSVYVDGARRQRGTGRQLYTALLGRLRELGYRTVLAVVV
EEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEE
TPNAASEKLHRTMGFEQVALYRRIGYKLGSWHDVTHFQLFLTSAGDSRAPGPAPGEA
CCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2315036 [H]