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The map label for this gene is yafV [C]

Identifier: 169630005

GI number: 169630005

Start: 2975716

End: 2976546

Strand: Reverse

Name: yafV [C]

Synonym: MAB_2922c

Alternate gene names: 169630005

Gene position: 2976546-2975716 (Counterclockwise)

Preceding gene: 169630006

Following gene: 169630003

Centisome position: 58.74

GC content: 60.77

Gene sequence:

>831_bases
TTGATTTCCACGAAACTGACAGTCGCGGGCCTGCAGTCGGCCGGGAGCCCGGCGAACCCGGAAGCCAACCTTGCCGAACT
CGATCAGGCCGCCAAAAGGCTAGCCGGACAAGCAGATCTACTCATCACGCCGGAGTTATTCGTGACCGGATACGACGTCG
GGGATCGTTTACCGGAATTGGTGAGCGCACACCATCTCGATGCGGTCCGTGCGATCGCTGAACAGAACAAGATCGCAATC
ATTGTGGGGATGGCCGAAAGTGTTGCGGCGGGGGAGCTGTACAACAACGCCGTCTTCGTCGATGAGCGGGGCGTCGTGCG
TGGCCGCCACCACAAGACACATTTGTTCGGCGAGATTGATCGTGCGTACTTCACGCCGGGGAGCCACGCGGTGACCATGG
TGCCGTATCGAGGCGTCAACATCGCCATGATGATCTGTTATGACGTGGAGTTTCCCGAAAATGCCCGGATGAGTGCCCTG
GCCGGTGCGCACCTGCTCGCGGTGCCGACGGCACAGATGACACCTTTCGAGTTCGTCGCCGATGTCGTGATCCGTACCCG
CGCGTGGGAGAACCAGATCTATCTGGCTTACATCAACCACGACGGTGTCGAGAACGCGACGACATACGTCGGCCGGAGCA
GCATCGTCGGTCCCGACGGGGGCGTGCTGGACCGTATCGAATCCGGAACCGGCACGATCATCGCCGAGATCGACACGGAC
GTGGTTCGATCAGCGCAGCGGGCCAACCCTTATCTCACCGATACGCGTCCCGAACTGAATTCGGCGCTTACGGCCGGCGG
TGGCTGCGTCACCTGGTCAAACAACGGTTGA

Upstream 100 bases:

>100_bases
ACACACTGCGGCTGCCGGAGGGAACGACGGCGGCAAGTAACGCGGAACTGGTCGCTGCTGGGCATGGCCGGATACGCACC
GCCACTACACGGGAGGTTTG

Downstream 100 bases:

>100_bases
CCCGCAAAAGTCGTGGTGCGCGATACTGGGATTGAACCAGTGACCTCTTCCGTGTCAGGGAAGCGCTCTCCCGCTGAGCT
AATCGCGCGGGTCTTTAATG

Product: carbon-nitrogen hydrolase family protein/ nitrilase/cyanide hydratase

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MISTKLTVAGLQSAGSPANPEANLAELDQAAKRLAGQADLLITPELFVTGYDVGDRLPELVSAHHLDAVRAIAEQNKIAI
IVGMAESVAAGELYNNAVFVDERGVVRGRHHKTHLFGEIDRAYFTPGSHAVTMVPYRGVNIAMMICYDVEFPENARMSAL
AGAHLLAVPTAQMTPFEFVADVVIRTRAWENQIYLAYINHDGVENATTYVGRSSIVGPDGGVLDRIESGTGTIIAEIDTD
VVRSAQRANPYLTDTRPELNSALTAGGGCVTWSNNG

Sequences:

>Translated_276_residues
MISTKLTVAGLQSAGSPANPEANLAELDQAAKRLAGQADLLITPELFVTGYDVGDRLPELVSAHHLDAVRAIAEQNKIAI
IVGMAESVAAGELYNNAVFVDERGVVRGRHHKTHLFGEIDRAYFTPGSHAVTMVPYRGVNIAMMICYDVEFPENARMSAL
AGAHLLAVPTAQMTPFEFVADVVIRTRAWENQIYLAYINHDGVENATTYVGRSSIVGPDGGVLDRIESGTGTIIAEIDTD
VVRSAQRANPYLTDTRPELNSALTAGGGCVTWSNNG
>Mature_276_residues
MISTKLTVAGLQSAGSPANPEANLAELDQAAKRLAGQADLLITPELFVTGYDVGDRLPELVSAHHLDAVRAIAEQNKIAI
IVGMAESVAAGELYNNAVFVDERGVVRGRHHKTHLFGEIDRAYFTPGSHAVTMVPYRGVNIAMMICYDVEFPENARMSAL
AGAHLLAVPTAQMTPFEFVADVVIRTRAWENQIYLAYINHDGVENATTYVGRSSIVGPDGGVLDRIESGTGTIIAEIDTD
VVRSAQRANPYLTDTRPELNSALTAGGGCVTWSNNG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=181, Percent_Identity=31.4917127071823, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI5031947, Length=181, Percent_Identity=31.4917127071823, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI297632348, Length=181, Percent_Identity=31.4917127071823, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI9910460, Length=237, Percent_Identity=26.5822784810127, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1786412, Length=252, Percent_Identity=26.1904761904762, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17556280, Length=253, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=289, Percent_Identity=26.2975778546713, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17933642, Length=268, Percent_Identity=27.9850746268657, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21355835, Length=277, Percent_Identity=23.4657039711191, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29563; Mature: 29563

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISTKLTVAGLQSAGSPANPEANLAELDQAAKRLAGQADLLITPELFVTGYDVGDRLPEL
CCCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCEEEEEECCHHHHHHHH
VSAHHLDAVRAIAEQNKIAIIVGMAESVAAGELYNNAVFVDERGVVRGRHHKTHLFGEID
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCEEEEECCCCCCCCCCCHHHHCCCC
RAYFTPGSHAVTMVPYRGVNIAMMICYDVEFPENARMSALAGAHLLAVPTAQMTPFEFVA
CEEECCCCCEEEEEEECCCEEEEEEEEECCCCCCCCHHHHCCCEEEEECCCCCCHHHHHH
DVVIRTRAWENQIYLAYINHDGVENATTYVGRSSIVGPDGGVLDRIESGTGTIIAEIDTD
HHHHHHEECCCEEEEEEECCCCCCCHHEECCCCCCCCCCCCHHHHCCCCCCEEEEECCHH
VVRSAQRANPYLTDTRPELNSALTAGGGCVTWSNNG
HHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEECCCC
>Mature Secondary Structure
MISTKLTVAGLQSAGSPANPEANLAELDQAAKRLAGQADLLITPELFVTGYDVGDRLPEL
CCCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCEEEEEECCHHHHHHHH
VSAHHLDAVRAIAEQNKIAIIVGMAESVAAGELYNNAVFVDERGVVRGRHHKTHLFGEID
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCEEEEECCCCCCCCCCCHHHHCCCC
RAYFTPGSHAVTMVPYRGVNIAMMICYDVEFPENARMSALAGAHLLAVPTAQMTPFEFVA
CEEECCCCCEEEEEEECCCEEEEEEEEECCCCCCCCHHHHCCCEEEEECCCCCCHHHHHH
DVVIRTRAWENQIYLAYINHDGVENATTYVGRSSIVGPDGGVLDRIESGTGTIIAEIDTD
HHHHHHEECCCEEEEEEECCCCCCCHHEECCCCCCCCCCCCHHHHCCCCCCEEEEECCHH
VVRSAQRANPYLTDTRPELNSALTAGGGCVTWSNNG
HHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8526497 [H]