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The map label for this gene is pyrB

Identifier: 169629915

GI number: 169629915

Start: 2883029

End: 2883982

Strand: Reverse

Name: pyrB

Synonym: MAB_2831c

Alternate gene names: 169629915

Gene position: 2883982-2883029 (Counterclockwise)

Preceding gene: 169629916

Following gene: 169629914

Centisome position: 56.92

GC content: 67.61

Gene sequence:

>954_bases
ATGAGACATCTCCTCTCCGCGGCCGACCTCACGCGTGACGAGGCCACCGCCATCCTCGACGACGCCGACCGGTTCCTGCA
GGCGCTAGCCGGGCGTGAGGTCAAGAAGCTGCCGACCCTGCGCGGCCGCACCATCATCACGATGTTCTATGAGAACTCCA
CGCGCACAAGGGTTTCCTTCGAGGTCGCGGGCAAGTGGATGAGCGCGGACGTCATCAACGTCAGTGCCTCCGGTTCCTCG
GTGGCCAAGGGGGAGTCGCTGCGCGACACCGCGTTGACCCTGCGCGCGATCGGCGCCGACGCGTTGATCGTCCGCCACCC
GGCCTCCGGGGTAGCCGCACAGCTGGCGCAGTGGACCGCCGAAGGTGAGGGCGGTCCGGCCGTCGTCAATGCGGGTGACG
GCACGCATGAGCACCCCACCCAGGCGTTACTCGACGCACTGACGATCCGGCAGCGGCTTGGCAGTCTCGAAGGGCGCCGC
GTCGTCATCGTCGGAGACATCCTGCACAGCCGGGTCGCCCGGTCGAATGCTTTGTTGCTCAACACGTTCGGCGCAGAGGT
GGTGCTGGTGGCGCCGCCGACGCTGCTGCCCGTCGGTGTCGGGACCTGGCCGGTGACGGTGACCCACGACCTGGACGCCG
AGTTGCCGGGGGCCGACGCGGTGCTGATGCTGCGGGTCCAGGCCGAGCGCATGACCGGCGGCTTTTTTCCGTCGGCCCGC
GAGTACTCGGTCCTATACGGCATGTCCGAGGCCAGGCAGCGGCTGCTGCCCGAACACGCGGTAGTGCTGCACCCCGGACC
CATGCTGCGCGGCATGGAGATCGCGTCCGCGGTGGCTGATTCTTCGCAATCCGGTGTTCTGCAACAGGTATCGAATGGAG
TGCATATCCGCATGGCCGTGCTGTTCCACCTGCTGGTCGGGTCCTCCGATAGCCCCGAGGCGGTTCCGGCATGA

Upstream 100 bases:

>100_bases
AGAACGTGCCGACGGCACGCTCGGAGGACGTCAAGGTGCTGCTGGCCGAACACGACGGCCGCGACGCGGTGATCATCACG
AGCGGGCAAGGGGCGCAGAC

Downstream 100 bases:

>100_bases
GCGTGTTGATCAAGGGCGTACTGCTGTACGGCGAGGGTGACCAGGTCGACGTCCTCATCGAGGACGGTCAGATCACCCGC
ATCGAGGCCGGCATCGGCGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRHLLSAADLTRDEATAILDDADRFLQALAGREVKKLPTLRGRTIITMFYENSTRTRVSFEVAGKWMSADVINVSASGSS
VAKGESLRDTALTLRAIGADALIVRHPASGVAAQLAQWTAEGEGGPAVVNAGDGTHEHPTQALLDALTIRQRLGSLEGRR
VVIVGDILHSRVARSNALLLNTFGAEVVLVAPPTLLPVGVGTWPVTVTHDLDAELPGADAVLMLRVQAERMTGGFFPSAR
EYSVLYGMSEARQRLLPEHAVVLHPGPMLRGMEIASAVADSSQSGVLQQVSNGVHIRMAVLFHLLVGSSDSPEAVPA

Sequences:

>Translated_317_residues
MRHLLSAADLTRDEATAILDDADRFLQALAGREVKKLPTLRGRTIITMFYENSTRTRVSFEVAGKWMSADVINVSASGSS
VAKGESLRDTALTLRAIGADALIVRHPASGVAAQLAQWTAEGEGGPAVVNAGDGTHEHPTQALLDALTIRQRLGSLEGRR
VVIVGDILHSRVARSNALLLNTFGAEVVLVAPPTLLPVGVGTWPVTVTHDLDAELPGADAVLMLRVQAERMTGGFFPSAR
EYSVLYGMSEARQRLLPEHAVVLHPGPMLRGMEIASAVADSSQSGVLQQVSNGVHIRMAVLFHLLVGSSDSPEAVPA
>Mature_317_residues
MRHLLSAADLTRDEATAILDDADRFLQALAGREVKKLPTLRGRTIITMFYENSTRTRVSFEVAGKWMSADVINVSASGSS
VAKGESLRDTALTLRAIGADALIVRHPASGVAAQLAQWTAEGEGGPAVVNAGDGTHEHPTQALLDALTIRQRLGSLEGRR
VVIVGDILHSRVARSNALLLNTFGAEVVLVAPPTLLPVGVGTWPVTVTHDLDAELPGADAVLMLRVQAERMTGGFFPSAR
EYSVLYGMSEARQRLLPEHAVVLHPGPMLRGMEIASAVADSSQSGVLQQVSNGVHIRMAVLFHLLVGSSDSPEAVPA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=315, Percent_Identity=34.9206349206349, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=32.8025477707006, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786469, Length=347, Percent_Identity=27.3775216138329, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790703, Length=342, Percent_Identity=28.0701754385965, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=333, Percent_Identity=32.4324324324324, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=314, Percent_Identity=32.1656050955414, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24642586, Length=321, Percent_Identity=33.6448598130841, Blast_Score=146, Evalue=2e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_MYCA9 (B1MCD9)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703564.1
- ProteinModelPortal:   B1MCD9
- SMR:   B1MCD9
- EnsemblBacteria:   EBMYCT00000006803
- GeneID:   5965339
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2831c
- GeneTree:   EBGT00050000016463
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   MABS561007:MAB_2831C-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33651; Mature: 33651

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLLSAADLTRDEATAILDDADRFLQALAGREVKKLPTLRGRTIITMFYENSTRTRVSF
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEEECCCCEEEEE
EVAGKWMSADVINVSASGSSVAKGESLRDTALTLRAIGADALIVRHPASGVAAQLAQWTA
EECCCEEECEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHC
EGEGGPAVVNAGDGTHEHPTQALLDALTIRQRLGSLEGRRVVIVGDILHSRVARSNALLL
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCEEEE
NTFGAEVVLVAPPTLLPVGVGTWPVTVTHDLDAELPGADAVLMLRVQAERMTGGFFPSAR
EECCCEEEEEECCCEEEECCCCCEEEEEECCCCCCCCCCEEEEEEEEHHHHCCCCCCCCC
EYSVLYGMSEARQRLLPEHAVVLHPGPMLRGMEIASAVADSSQSGVLQQVSNGVHIRMAV
CCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCEEHHHH
LFHLLVGSSDSPEAVPA
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRHLLSAADLTRDEATAILDDADRFLQALAGREVKKLPTLRGRTIITMFYENSTRTRVSF
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEEECCCCEEEEE
EVAGKWMSADVINVSASGSSVAKGESLRDTALTLRAIGADALIVRHPASGVAAQLAQWTA
EECCCEEECEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHC
EGEGGPAVVNAGDGTHEHPTQALLDALTIRQRLGSLEGRRVVIVGDILHSRVARSNALLL
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCEEEE
NTFGAEVVLVAPPTLLPVGVGTWPVTVTHDLDAELPGADAVLMLRVQAERMTGGFFPSAR
EECCCEEEEEECCCEEEECCCCCEEEEEECCCCCCCCCCEEEEEEEEHHHHCCCCCCCCC
EYSVLYGMSEARQRLLPEHAVVLHPGPMLRGMEIASAVADSSQSGVLQQVSNGVHIRMAV
CCCEEECHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCEEHHHH
LFHLLVGSSDSPEAVPA
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA