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The map label for this gene is bioB

Identifier: 169629769

GI number: 169629769

Start: 2726137

End: 2727165

Strand: Reverse

Name: bioB

Synonym: MAB_2684c

Alternate gene names: 169629769

Gene position: 2727165-2726137 (Counterclockwise)

Preceding gene: 169629771

Following gene: 169629768

Centisome position: 53.82

GC content: 65.79

Gene sequence:

>1029_bases
GTGACTGAGACAGCCGAGCTGACAGCCGCGACGGACGCCGACGTATTGGCAGTCGCCCGTGAGCAGGTCTTGGAACAAGG
CGTGGGCCTGACGCAGGAGCAGGTGCTGCGAGTCCTGCAACTTCCTGACGATCGCTTGGAAGAGCTGCTCGCGCTCGCTC
ACGAGGTGCGCATGCGCTGGTGCGGCCCGGAGGTCGAGGTTGAGGGCATCATCAGCCTCAAGACCGGCGGTTGCCCCGAG
GACTGCCATTTCTGTTCGCAGTCGGGTCTGTTCGCTTCCCCGGTGCGCAGCGCGTGGCTCGACATCCCGAGCCTGGTGGA
GGCCGCCAAGCAGACCGCAAAGTCCGGTGCTACCGAGTTCTGCATCGTGGCGGCGGTCCGTGGGCCCGACGAGCGTCTGC
TCGCGCAGGTTGCCGCCGGTATCGAAGCCATCCGCAATGAGGTCGACATCCAGATCGCCTGCTCGCTGGGCATGCTGACA
CAGGAGCAGGTCGATCGTCTGTCGGCGATGGGCGTGCACCGCTACAACCACAACCTGGAGACGGCGAAGTCGCACTTCCC
CAACGTGGTCACCACGCACAGCTGGGAAGAGCGTTGGGACACCCTGAAGATGGTGCGCGAGGCAGGCATGGAGGTCTGCT
GTGGTGGCATCCTCGGAATGGGCGAAACCCTGGAGCAGCGCGCGGAATTCGCCGCGAACCTGGCCGAACTGGAGCCCGAC
GAGGTTCCGCTGAACTTCCTCAACCCGCGTCCGGGAACGCCGTTCGGTGATCTTGAGGTGCTGCCGGCATCGGACGCGCT
GCGCGCGGTCGCGGCGTTCCGGCTGGCGTTGCCGCGCACCATGCTGCGGTTCGCCGGTGGACGCGAGATCACCCTGGGCG
ACCTGGGCGCCAAGCAGGGCATCTTGGGTGGAATCAACGCCGTCATCGTCGGCAACTACCTGACCACGCTGGGACGTCCG
GCCGAGGCGGACTTGGAGCTGCTCGACGATCTGCAGATGCCGATCAAGGCTCTGAACAGCAGCCTGTGA

Upstream 100 bases:

>100_bases
CGGGGCTGTGCGCCCCTTGTTTGGCTCAGTGATGGCCATACTCTAACCTGAACACCGTTCAGGATTGTGTATCAGGTTTG
TACCTAGTGAGGAGCCCTGC

Downstream 100 bases:

>100_bases
CCAATAGCCTGTACCTCATGGGTACCGAACCGGTGGCTGCCGGTAAGTACAACGTGTACACCGGGATCGAGATCGATGGC
GGCGCTGCTGGTGCGCTGCT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTETAELTAATDADVLAVAREQVLEQGVGLTQEQVLRVLQLPDDRLEELLALAHEVRMRWCGPEVEVEGIISLKTGGCPE
DCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLT
QEQVDRLSAMGVHRYNHNLETAKSHFPNVVTTHSWEERWDTLKMVREAGMEVCCGGILGMGETLEQRAEFAANLAELEPD
EVPLNFLNPRPGTPFGDLEVLPASDALRAVAAFRLALPRTMLRFAGGREITLGDLGAKQGILGGINAVIVGNYLTTLGRP
AEADLELLDDLQMPIKALNSSL

Sequences:

>Translated_342_residues
MTETAELTAATDADVLAVAREQVLEQGVGLTQEQVLRVLQLPDDRLEELLALAHEVRMRWCGPEVEVEGIISLKTGGCPE
DCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLT
QEQVDRLSAMGVHRYNHNLETAKSHFPNVVTTHSWEERWDTLKMVREAGMEVCCGGILGMGETLEQRAEFAANLAELEPD
EVPLNFLNPRPGTPFGDLEVLPASDALRAVAAFRLALPRTMLRFAGGREITLGDLGAKQGILGGINAVIVGNYLTTLGRP
AEADLELLDDLQMPIKALNSSL
>Mature_341_residues
TETAELTAATDADVLAVAREQVLEQGVGLTQEQVLRVLQLPDDRLEELLALAHEVRMRWCGPEVEVEGIISLKTGGCPED
CHFCSQSGLFASPVRSAWLDIPSLVEAAKQTAKSGATEFCIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLTQ
EQVDRLSAMGVHRYNHNLETAKSHFPNVVTTHSWEERWDTLKMVREAGMEVCCGGILGMGETLEQRAEFAANLAELEPDE
VPLNFLNPRPGTPFGDLEVLPASDALRAVAAFRLALPRTMLRFAGGREITLGDLGAKQGILGGINAVIVGNYLTTLGRPA
EADLELLDDLQMPIKALNSSL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=304, Percent_Identity=36.1842105263158, Blast_Score=191, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6321725, Length=339, Percent_Identity=27.7286135693215, Blast_Score=155, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_MYCA9 (B1MBZ3)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703418.1
- ProteinModelPortal:   B1MBZ3
- SMR:   B1MBZ3
- EnsemblBacteria:   EBMYCT00000007956
- GeneID:   5965193
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2684c
- GeneTree:   EBGT00050000017018
- HOGENOM:   HBG405569
- OMA:   ATGATEF
- ProtClustDB:   PRK06256
- BioCyc:   MABS561007:MAB_2684C-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36961; Mature: 36830

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETAELTAATDADVLAVAREQVLEQGVGLTQEQVLRVLQLPDDRLEELLALAHEVRMRW
CCCCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CGPEVEVEGIISLKTGGCPEDCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTAKSGATEF
CCCCEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCE
CIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLTQEQVDRLSAMGVHRYNHNLE
EEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHH
TAKSHFPNVVTTHSWEERWDTLKMVREAGMEVCCGGILGMGETLEQRAEFAANLAELEPD
HHHHHCCCCEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
EVPLNFLNPRPGTPFGDLEVLPASDALRAVAAFRLALPRTMLRFAGGREITLGDLGAKQG
CCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
ILGGINAVIVGNYLTTLGRPAEADLELLDDLQMPIKALNSSL
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TETAELTAATDADVLAVAREQVLEQGVGLTQEQVLRVLQLPDDRLEELLALAHEVRMRW
CCCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CGPEVEVEGIISLKTGGCPEDCHFCSQSGLFASPVRSAWLDIPSLVEAAKQTAKSGATEF
CCCCEEEEEEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCE
CIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLTQEQVDRLSAMGVHRYNHNLE
EEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHH
TAKSHFPNVVTTHSWEERWDTLKMVREAGMEVCCGGILGMGETLEQRAEFAANLAELEPD
HHHHHCCCCEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
EVPLNFLNPRPGTPFGDLEVLPASDALRAVAAFRLALPRTMLRFAGGREITLGDLGAKQG
CCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
ILGGINAVIVGNYLTTLGRPAEADLELLDDLQMPIKALNSSL
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA