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The map label for this gene is trpA

Identifier: 169629728

GI number: 169629728

Start: 2683915

End: 2684703

Strand: Reverse

Name: trpA

Synonym: MAB_2643c

Alternate gene names: 169629728

Gene position: 2684703-2683915 (Counterclockwise)

Preceding gene: 169629729

Following gene: 169629727

Centisome position: 52.98

GC content: 69.07

Gene sequence:

>789_bases
ATGACACAGCCCGGCCGTCTGACCGAGGTCTTCGACAAGTGCCGCGCCGAAGAACGCGCCGCGCTGATCGGGTACCTGCC
CAACGGTTACCCGGACCTGGACACCTCGATCGAACTGATGACCACCCTGGTGCGGGGCGGGTGCGACATCATCGAAGTGG
GGATCGCGTACTCGGATCCGATGATGGACGGCCCGATGATCGCCGCCGCAGCGGAAACGGCGCTCGCCAACGGGGTTCGG
GTGGCCGACGTCTTCACCACCGTCCGCGCCATCACCGATGCCGGCGGTCACGCGGTCGTCATGTCCTACTGGAACCCGGT
GCTGCGTTACGGCGTTGACGCGTTCGCACGTGAGCTCGCCGCGGCGGGTGGCCTCGGAATCATCACACCGGATCTGATCC
CGGACGAGGCCGATGACTGGATCGCCGCCTCGGACGCTCATGACCTGGACCGGATCTTTCTTGTCGCCCCGTCGTCCACG
CCCGAAAGGCTGGAGAGGACCATCGGCGCCTGCCGGGGATTTGTCTACGCGGCGTCGACCATGGGTGTCACGGGTGCGCG
TGACGCGGTATCCAATGCCGCACCCGCCCTGGTGAGCAGGGTCCGTGAGGTCTCCGATATCGCGGTGGGGGTCGGACTCG
GCGTGCGGTCGGGCGCGCAGGCGGCCGAGATCGCGGCCTACGCCGACGGCGTCATCGTCGGGTCGGCGTTGGTGGCGGCG
GCCCCGCAGGGGCCGGCCGCCGTGCGCAGCCTGGCCGAGGAGCTGGCCACCGGAGTCCGAAGGGCATGA

Upstream 100 bases:

>100_bases
AGGGCGCGGTCATCGTGGTGAGCCTGTCGGGTCGCGGCGACAAGGATGTCGAGACCGCCGCCAAGTGGTTCGACCTACTG
GAGAAGGACGGCAGCCAGTC

Downstream 100 bases:

>100_bases
GTTCAGGGAAAACAGGTAGAGCACTGTGACTGTCGTGAATCTGGCATACATCCCCAGTCCACCCCAAGGGGTTTGGCATC
TTGGGCCGATCCCGATCCGC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTQPGRLTEVFDKCRAEERAALIGYLPNGYPDLDTSIELMTTLVRGGCDIIEVGIAYSDPMMDGPMIAAAAETALANGVR
VADVFTTVRAITDAGGHAVVMSYWNPVLRYGVDAFARELAAAGGLGIITPDLIPDEADDWIAASDAHDLDRIFLVAPSST
PERLERTIGACRGFVYAASTMGVTGARDAVSNAAPALVSRVREVSDIAVGVGLGVRSGAQAAEIAAYADGVIVGSALVAA
APQGPAAVRSLAEELATGVRRA

Sequences:

>Translated_262_residues
MTQPGRLTEVFDKCRAEERAALIGYLPNGYPDLDTSIELMTTLVRGGCDIIEVGIAYSDPMMDGPMIAAAAETALANGVR
VADVFTTVRAITDAGGHAVVMSYWNPVLRYGVDAFARELAAAGGLGIITPDLIPDEADDWIAASDAHDLDRIFLVAPSST
PERLERTIGACRGFVYAASTMGVTGARDAVSNAAPALVSRVREVSDIAVGVGLGVRSGAQAAEIAAYADGVIVGSALVAA
APQGPAAVRSLAEELATGVRRA
>Mature_261_residues
TQPGRLTEVFDKCRAEERAALIGYLPNGYPDLDTSIELMTTLVRGGCDIIEVGIAYSDPMMDGPMIAAAAETALANGVRV
ADVFTTVRAITDAGGHAVVMSYWNPVLRYGVDAFARELAAAGGLGIITPDLIPDEADDWIAASDAHDLDRIFLVAPSSTP
ERLERTIGACRGFVYAASTMGVTGARDAVSNAAPALVSRVREVSDIAVGVGLGVRSGAQAAEIAAYADGVIVGSALVAAA
PQGPAAVRSLAEELATGVRRA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=219, Percent_Identity=26.9406392694064, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321412, Length=258, Percent_Identity=35.2713178294574, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_MYCA9 (B1MBV2)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703377.1
- ProteinModelPortal:   B1MBV2
- SMR:   B1MBV2
- EnsemblBacteria:   EBMYCT00000005126
- GeneID:   5965152
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2643c
- GeneTree:   EBGT00050000016358
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   MABS561007:MAB_2643C-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27046; Mature: 26915

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 52-52 ACT_SITE 63-63

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPGRLTEVFDKCRAEERAALIGYLPNGYPDLDTSIELMTTLVRGGCDIIEVGIAYSDP
CCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCEECCC
MMDGPMIAAAAETALANGVRVADVFTTVRAITDAGGHAVVMSYWNPVLRYGVDAFARELA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AAGGLGIITPDLIPDEADDWIAASDAHDLDRIFLVAPSSTPERLERTIGACRGFVYAAST
HCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
MGVTGARDAVSNAAPALVSRVREVSDIAVGVGLGVRSGAQAAEIAAYADGVIVGSALVAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHC
APQGPAAVRSLAEELATGVRRA
CCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQPGRLTEVFDKCRAEERAALIGYLPNGYPDLDTSIELMTTLVRGGCDIIEVGIAYSDP
CCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCEECCC
MMDGPMIAAAAETALANGVRVADVFTTVRAITDAGGHAVVMSYWNPVLRYGVDAFARELA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AAGGLGIITPDLIPDEADDWIAASDAHDLDRIFLVAPSSTPERLERTIGACRGFVYAAST
HCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
MGVTGARDAVSNAAPALVSRVREVSDIAVGVGLGVRSGAQAAEIAAYADGVIVGSALVAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHC
APQGPAAVRSLAEELATGVRRA
CCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA