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The map label for this gene is lcfB [H]

Identifier: 169629660

GI number: 169629660

Start: 2618439

End: 2620328

Strand: Reverse

Name: lcfB [H]

Synonym: MAB_2574c

Alternate gene names: 169629660

Gene position: 2620328-2618439 (Counterclockwise)

Preceding gene: 169629661

Following gene: 169629659

Centisome position: 51.71

GC content: 64.87

Gene sequence:

>1890_bases
GTGAAGTCCGACGATCTATTGTGGCCTTGCTACCGCGGGCCCGAGGATCTGGCCGCGATCGAATCGATCCCGTTGTCGCA
GCGCGGCCTGCCGGTATCGACCTATGCGCTGCTGCACCGGGCAGCCAGGATGTGGCCAGATAGATCGGCAGTGACGGTGA
TCCCCGATACCACGCGGTGGCGTGAACCGATGCGCCGCACCTTCAGCCAGTTGCTCGCCGACGTGCATCGCACGGCGAAC
CTGCTCGCCGATGTCGGTGTCGGCCGTACCGACGCGGTCGCGCTGATTGCCCCCAACTGCGCCCAACTCATCACCGCGAC
CCTGGCCGCGCAACTCGCCGGCATCGCGGCACCGATCAACGGTGCCCTGTCCGCCGACCACGTCACCGAACTGGTGCAGC
GTTCGGGGGCAAGAGTTCTCATCGCCGCCGCACCGCAGCTCGATCAGAACTGTTGGGATCTGGCCGAACAGTTGGCCCGG
CGCGGCGTTGTCGATACCGTGCTGGTCTTGGCGGCGTCAGAAGGCGAACCCACCGCGGCTGTGCCTGCGGTGATCGGTCA
CGCCAGGGTGGGCTACCTTGCCGAACTCGCTGAATCCTATGACAGCACCAGGTTCTCTGGCACCCTGCCGGCGGCGGCTG
ATCTGGCGGCGCTCTTTCACACCGGCGGCACCACCGGAACACCGAAGCTGGCCGCCCACACTCACGCCAACGAAGTGTCC
GACGCCTGGATGATCGCGGCGAGCTCACTGCTCGATGAGAACTCGGTGGCCTTGGCGGCGCTACCGCTGTTCCACGTCAA
CGCGTTGGTCGTGACGATCCTGGCACCGCTGTTGCGCGGCCAGCACACGGTGTGGGCCGGCCCACTGGGCTTTCGCGACC
CCGCGCTCTACGCGCATCTGTGGAAGATCGTGCAGCACTACGACATCAACATCATCAGCGCCGTGCCCACGGTCTACTCG
GTGCTGTCTCAGCTCCCGGTCGATGCCGACATCACCAGCTTGCAATTCGCCCTAGTAGGTGCCTCGGCACTACCGGAGTC
GGTGCGCCGGAGCTTTCAGTCCCACACCGGCGTGCCGCTGGTGCAGGGATACGGGTTGACCGAAGCCACCTGCGCGAGCA
TCCGCAGCTTCCCGGATCATCTTCGCCCCGGATCGGTAGGTCAACGGCTGCCATACCAACAGGTCAAAATTGCCCGCAAG
AGCGAGGCCCGATGGCAGAATCTGCCGGCGGGCTCTGTCGGACACCTACTTATCAATGGGCCCAACGTTTTTCCAGGCTA
TGTCACCGCACGTACCGACATCGGCTTCGAACTCGATGGGTGCGGGGTGCTGGACGGCGGCTGGCTCGACACCGGGGACT
TGGCGCGGATCGATGCCGAAGGCTACGTGTTCCTGGCGGGACGGACCAAGGACTTGATCATTCGCGGCGGCCACAACATC
GATCCAGCTACCACCGAAGATGCGCTGCTATCGCATCCGGCGGTAACCGGTGCCGCCGCCGTCGGCCGACCCGACCCGCA
CGCCGGCGAGATCCCGGTCGCGTACGTCACCGTCGCCGCCAACACCCAGGTAAGCCATCAACAGTTACAAGACTGGGCCA
CAACGCATGTCACTGAACCAACGGCGGCACCCAAAGTGGTCACCATTGTCGACGCACTTCCTGTCACGGCAATCGGCAAA
CCGCACAAATTGCCACTACGCGCCGACGCCACCCGGGTCGCACTACAGCATGCACTGACCGGAATCGGTGGTGTACATGC
TGTTGAGGCCGGCGTCGAGGACGGCACCGTCACCGCGATGGTGACCGTCGGCGAGGACGCTGACACTACCGCCATCCAGA
CAATCCTCGACTGCTACGCGGTCACTGCGCAGATCCGGCGCCCGTCGTGA

Upstream 100 bases:

>100_bases
ACCCCAAGTACCTGCACGACGGCGACGTGGTGGAGGCCTCGGTGGCCACCGATGACGGCAGCATCTGCCTGGGAACCCAG
CGCACCACGGTGAGATTCTC

Downstream 100 bases:

>100_bases
ACGCCTCAGCACAGCTGTCGTTTCCGGTTGTCATCATCGGCGCGGGACCAACCGGCGTCACCGCCGCGACGCTGTTGGCT
CAGCACGGCGTGCAGTGCCT

Product: AMP-binding domain protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MKSDDLLWPCYRGPEDLAAIESIPLSQRGLPVSTYALLHRAARMWPDRSAVTVIPDTTRWREPMRRTFSQLLADVHRTAN
LLADVGVGRTDAVALIAPNCAQLITATLAAQLAGIAAPINGALSADHVTELVQRSGARVLIAAAPQLDQNCWDLAEQLAR
RGVVDTVLVLAASEGEPTAAVPAVIGHARVGYLAELAESYDSTRFSGTLPAAADLAALFHTGGTTGTPKLAAHTHANEVS
DAWMIAASSLLDENSVALAALPLFHVNALVVTILAPLLRGQHTVWAGPLGFRDPALYAHLWKIVQHYDINIISAVPTVYS
VLSQLPVDADITSLQFALVGASALPESVRRSFQSHTGVPLVQGYGLTEATCASIRSFPDHLRPGSVGQRLPYQQVKIARK
SEARWQNLPAGSVGHLLINGPNVFPGYVTARTDIGFELDGCGVLDGGWLDTGDLARIDAEGYVFLAGRTKDLIIRGGHNI
DPATTEDALLSHPAVTGAAAVGRPDPHAGEIPVAYVTVAANTQVSHQQLQDWATTHVTEPTAAPKVVTIVDALPVTAIGK
PHKLPLRADATRVALQHALTGIGGVHAVEAGVEDGTVTAMVTVGEDADTTAIQTILDCYAVTAQIRRPS

Sequences:

>Translated_629_residues
MKSDDLLWPCYRGPEDLAAIESIPLSQRGLPVSTYALLHRAARMWPDRSAVTVIPDTTRWREPMRRTFSQLLADVHRTAN
LLADVGVGRTDAVALIAPNCAQLITATLAAQLAGIAAPINGALSADHVTELVQRSGARVLIAAAPQLDQNCWDLAEQLAR
RGVVDTVLVLAASEGEPTAAVPAVIGHARVGYLAELAESYDSTRFSGTLPAAADLAALFHTGGTTGTPKLAAHTHANEVS
DAWMIAASSLLDENSVALAALPLFHVNALVVTILAPLLRGQHTVWAGPLGFRDPALYAHLWKIVQHYDINIISAVPTVYS
VLSQLPVDADITSLQFALVGASALPESVRRSFQSHTGVPLVQGYGLTEATCASIRSFPDHLRPGSVGQRLPYQQVKIARK
SEARWQNLPAGSVGHLLINGPNVFPGYVTARTDIGFELDGCGVLDGGWLDTGDLARIDAEGYVFLAGRTKDLIIRGGHNI
DPATTEDALLSHPAVTGAAAVGRPDPHAGEIPVAYVTVAANTQVSHQQLQDWATTHVTEPTAAPKVVTIVDALPVTAIGK
PHKLPLRADATRVALQHALTGIGGVHAVEAGVEDGTVTAMVTVGEDADTTAIQTILDCYAVTAQIRRPS
>Mature_629_residues
MKSDDLLWPCYRGPEDLAAIESIPLSQRGLPVSTYALLHRAARMWPDRSAVTVIPDTTRWREPMRRTFSQLLADVHRTAN
LLADVGVGRTDAVALIAPNCAQLITATLAAQLAGIAAPINGALSADHVTELVQRSGARVLIAAAPQLDQNCWDLAEQLAR
RGVVDTVLVLAASEGEPTAAVPAVIGHARVGYLAELAESYDSTRFSGTLPAAADLAALFHTGGTTGTPKLAAHTHANEVS
DAWMIAASSLLDENSVALAALPLFHVNALVVTILAPLLRGQHTVWAGPLGFRDPALYAHLWKIVQHYDINIISAVPTVYS
VLSQLPVDADITSLQFALVGASALPESVRRSFQSHTGVPLVQGYGLTEATCASIRSFPDHLRPGSVGQRLPYQQVKIARK
SEARWQNLPAGSVGHLLINGPNVFPGYVTARTDIGFELDGCGVLDGGWLDTGDLARIDAEGYVFLAGRTKDLIIRGGHNI
DPATTEDALLSHPAVTGAAAVGRPDPHAGEIPVAYVTVAANTQVSHQQLQDWATTHVTEPTAAPKVVTIVDALPVTAIGK
PHKLPLRADATRVALQHALTGIGGVHAVEAGVEDGTVTAMVTVGEDADTTAIQTILDCYAVTAQIRRPS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=566, Percent_Identity=25.0883392226148, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI42544132, Length=588, Percent_Identity=25, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI187761345, Length=546, Percent_Identity=26.9230769230769, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI187761343, Length=546, Percent_Identity=26.9230769230769, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI58082049, Length=530, Percent_Identity=25.8490566037736, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI122937307, Length=616, Percent_Identity=24.6753246753247, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI157311624, Length=537, Percent_Identity=25.6983240223464, Blast_Score=120, Evalue=6e-27,
Organism=Homo sapiens, GI157311622, Length=537, Percent_Identity=25.6983240223464, Blast_Score=120, Evalue=6e-27,
Organism=Homo sapiens, GI115511026, Length=539, Percent_Identity=26.3450834879406, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI38505220, Length=546, Percent_Identity=24.3589743589744, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI13375727, Length=530, Percent_Identity=24.1509433962264, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI227499619, Length=533, Percent_Identity=23.6397748592871, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI8923896, Length=568, Percent_Identity=25, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI27477105, Length=187, Percent_Identity=29.9465240641711, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI253970489, Length=581, Percent_Identity=24.7848537005164, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI42794756, Length=318, Percent_Identity=25.4716981132075, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI42794760, Length=318, Percent_Identity=25.4716981132075, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI42794758, Length=318, Percent_Identity=25.4716981132075, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788107, Length=538, Percent_Identity=28.2527881040892, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI145693145, Length=371, Percent_Identity=31.5363881401617, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI221142682, Length=510, Percent_Identity=25.6862745098039, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1789201, Length=515, Percent_Identity=24.4660194174757, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786810, Length=369, Percent_Identity=30.0813008130081, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1790505, Length=529, Percent_Identity=25.7088846880907, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786529, Length=563, Percent_Identity=24.3339253996448, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=368, Percent_Identity=31.25, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=556, Percent_Identity=24.1007194244604, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=556, Percent_Identity=24.1007194244604, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=556, Percent_Identity=24.1007194244604, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17558820, Length=471, Percent_Identity=25.9023354564756, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=360, Percent_Identity=26.1111111111111, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=581, Percent_Identity=26.1617900172117, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=475, Percent_Identity=24.8421052631579, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=545, Percent_Identity=26.0550458715596, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=512, Percent_Identity=22.65625, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=582, Percent_Identity=23.8831615120275, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=356, Percent_Identity=25.561797752809, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=484, Percent_Identity=27.2727272727273, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319264, Length=591, Percent_Identity=23.3502538071066, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21355181, Length=504, Percent_Identity=25.1984126984127, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24581924, Length=530, Percent_Identity=25.8490566037736, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859661, Length=560, Percent_Identity=25.3571428571429, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI62472339, Length=544, Percent_Identity=25.1838235294118, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24667955, Length=544, Percent_Identity=25.1838235294118, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17933690, Length=536, Percent_Identity=24.2537313432836, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358303, Length=368, Percent_Identity=23.3695652173913, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24656500, Length=507, Percent_Identity=22.6824457593688, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281365686, Length=326, Percent_Identity=21.7791411042945, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24667959, Length=513, Percent_Identity=24.3664717348928, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356441, Length=479, Percent_Identity=19.2066805845511, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648257, Length=553, Percent_Identity=21.880650994575, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20130357, Length=350, Percent_Identity=24, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 66435; Mature: 66435

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDDLLWPCYRGPEDLAAIESIPLSQRGLPVSTYALLHRAARMWPDRSAVTVIPDTTRW
CCCCCCEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHH
REPMRRTFSQLLADVHRTANLLADVGVGRTDAVALIAPNCAQLITATLAAQLAGIAAPIN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCC
GALSADHVTELVQRSGARVLIAAAPQLDQNCWDLAEQLARRGVVDTVLVLAASEGEPTAA
CCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHEEEEEEECCCCCCHH
VPAVIGHARVGYLAELAESYDSTRFSGTLPAAADLAALFHTGGTTGTPKLAAHTHANEVS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHH
DAWMIAASSLLDENSVALAALPLFHVNALVVTILAPLLRGQHTVWAGPLGFRDPALYAHL
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
WKIVQHYDINIISAVPTVYSVLSQLPVDADITSLQFALVGASALPESVRRSFQSHTGVPL
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCE
VQGYGLTEATCASIRSFPDHLRPGSVGQRLPYQQVKIARKSEARWQNLPAGSVGHLLING
EECCCCCHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PNVFPGYVTARTDIGFELDGCGVLDGGWLDTGDLARIDAEGYVFLAGRTKDLIIRGGHNI
CCCCCCEEEEEECCCEEECCCEEECCCCCCCCCEEEECCCCEEEEECCCCEEEEECCCCC
DPATTEDALLSHPAVTGAAAVGRPDPHAGEIPVAYVTVAANTQVSHQQLQDWATTHVTEP
CCCCCHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCC
TAAPKVVTIVDALPVTAIGKPHKLPLRADATRVALQHALTGIGGVHAVEAGVEDGTVTAM
CCCCCEEEEECCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
VTVGEDADTTAIQTILDCYAVTAQIRRPS
EEECCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSDDLLWPCYRGPEDLAAIESIPLSQRGLPVSTYALLHRAARMWPDRSAVTVIPDTTRW
CCCCCCEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHH
REPMRRTFSQLLADVHRTANLLADVGVGRTDAVALIAPNCAQLITATLAAQLAGIAAPIN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCC
GALSADHVTELVQRSGARVLIAAAPQLDQNCWDLAEQLARRGVVDTVLVLAASEGEPTAA
CCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHEEEEEEECCCCCCHH
VPAVIGHARVGYLAELAESYDSTRFSGTLPAAADLAALFHTGGTTGTPKLAAHTHANEVS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHH
DAWMIAASSLLDENSVALAALPLFHVNALVVTILAPLLRGQHTVWAGPLGFRDPALYAHL
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
WKIVQHYDINIISAVPTVYSVLSQLPVDADITSLQFALVGASALPESVRRSFQSHTGVPL
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCE
VQGYGLTEATCASIRSFPDHLRPGSVGQRLPYQQVKIARKSEARWQNLPAGSVGHLLING
EECCCCCHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PNVFPGYVTARTDIGFELDGCGVLDGGWLDTGDLARIDAEGYVFLAGRTKDLIIRGGHNI
CCCCCCEEEEEECCCEEECCCEEECCCCCCCCCEEEECCCCEEEEECCCCEEEEECCCCC
DPATTEDALLSHPAVTGAAAVGRPDPHAGEIPVAYVTVAANTQVSHQQLQDWATTHVTEP
CCCCCHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCC
TAAPKVVTIVDALPVTAIGKPHKLPLRADATRVALQHALTGIGGVHAVEAGVEDGTVTAM
CCCCCEEEEECCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
VTVGEDADTTAIQTILDCYAVTAQIRRPS
EEECCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]