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The map label for this gene is ysgA [H]

Identifier: 169629410

GI number: 169629410

Start: 2378367

End: 2379119

Strand: Direct

Name: ysgA [H]

Synonym: MAB_2324

Alternate gene names: 169629410

Gene position: 2378367-2379119 (Clockwise)

Preceding gene: 169629409

Following gene: 169629411

Centisome position: 46.94

GC content: 65.34

Gene sequence:

>753_bases
GTGATCGACGCGGTCAAACTGCACCGGCCCGCCGCCCGTCGGCGGGCCGGTCTTTTTCTGGCAGAAGGCCCCAACCTCGT
CGAGGCCGCACTGCGTTCCGGTGCGGTTGAGCGGATTTTTGCGACGGATGCCGCGATAGAGCGATTTGTCGATCTGTTGG
CCGAAGCGCCCGTACGCCGGGTCACCGAGCGCGCTGCTAAAGCGTTGTCCGACACCGTGACTCCGGTGGGGCTGGTCGCC
CAGTGCCGCACTGTCGTATCCACCTGGTCTCAAGTCACCGCGCGATCGCCCCGATTTGTCATTGTCGCCGTGGATATCTC
GGAACCTGGCAACGCGGGCACCCTCATCCGGGTCGCGGACGCGATGGGTGCCGATGCCGTCGTACTGGCGGGCAATAGCG
TCGACCCCTTCAACGGCAAAAGCTTGCGTGCTTCGGCGGGAAGCATTTTCAACATTCCGGTGGTGCCCGAAGGGGACGTC
GGGGCGATCGCCGCCGATCTTGCGCAGACGGGGGTGGCTCTATTGGCCACCACTCTCGACGGTGACCTTTCCCTCGATGA
GGCGGACGCGGTCCTTGCGGGCCCCTGCGCTTGGTTCTTCGGAAACGAGGCCCATGGGCTCGACGGGGCGACCGCAGCCT
TGGCCACGCATCGGATCAACATCCCGATGCGTGGGGGAGCTGAGAGCCTCAACCTGGCCAGCGCCGCCTCCATTTGTCTG
TACGCCACGGCGCGGATGCATCGCCGTCAATAG

Upstream 100 bases:

>100_bases
TCGCCAAGGCCGCGCTGCCTGAGGATGTTAACGCTCCGGCCGGGGAAGCCGCCTGAGCATTCCGGCTGACTCAACGGCAC
TGACTGAGCGGTCACAGACA

Downstream 100 bases:

>100_bases
CCTCCAGTACTATCCGACAAAGCCGTAACGGCGAGTACTAGTAACTTCGATGGATCGAGGAACCTCATGCCCGCACCTCT
GCAGATCAGGCACTTTCGTG

Product: tRNA/rRNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MIDAVKLHRPAARRRAGLFLAEGPNLVEAALRSGAVERIFATDAAIERFVDLLAEAPVRRVTERAAKALSDTVTPVGLVA
QCRTVVSTWSQVTARSPRFVIVAVDISEPGNAGTLIRVADAMGADAVVLAGNSVDPFNGKSLRASAGSIFNIPVVPEGDV
GAIAADLAQTGVALLATTLDGDLSLDEADAVLAGPCAWFFGNEAHGLDGATAALATHRINIPMRGGAESLNLASAASICL
YATARMHRRQ

Sequences:

>Translated_250_residues
MIDAVKLHRPAARRRAGLFLAEGPNLVEAALRSGAVERIFATDAAIERFVDLLAEAPVRRVTERAAKALSDTVTPVGLVA
QCRTVVSTWSQVTARSPRFVIVAVDISEPGNAGTLIRVADAMGADAVVLAGNSVDPFNGKSLRASAGSIFNIPVVPEGDV
GAIAADLAQTGVALLATTLDGDLSLDEADAVLAGPCAWFFGNEAHGLDGATAALATHRINIPMRGGAESLNLASAASICL
YATARMHRRQ
>Mature_250_residues
MIDAVKLHRPAARRRAGLFLAEGPNLVEAALRSGAVERIFATDAAIERFVDLLAEAPVRRVTERAAKALSDTVTPVGLVA
QCRTVVSTWSQVTARSPRFVIVAVDISEPGNAGTLIRVADAMGADAVVLAGNSVDPFNGKSLRASAGSIFNIPVVPEGDV
GAIAADLAQTGVALLATTLDGDLSLDEADAVLAGPCAWFFGNEAHGLDGATAALATHRINIPMRGGAESLNLASAASICL
YATARMHRRQ

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=150, Percent_Identity=31.3333333333333, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1790623, Length=147, Percent_Identity=25.1700680272109, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 25903; Mature: 25903

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDAVKLHRPAARRRAGLFLAEGPNLVEAALRSGAVERIFATDAAIERFVDLLAEAPVRR
CCCCHHCCCCHHHHHHCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHH
VTERAAKALSDTVTPVGLVAQCRTVVSTWSQVTARSPRFVIVAVDISEPGNAGTLIRVAD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEC
AMGADAVVLAGNSVDPFNGKSLRASAGSIFNIPVVPEGDVGAIAADLAQTGVALLATTLD
CCCCCEEEEECCCCCCCCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEEECC
GDLSLDEADAVLAGPCAWFFGNEAHGLDGATAALATHRINIPMRGGAESLNLASAASICL
CCCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHH
YATARMHRRQ
HHHHHHHCCC
>Mature Secondary Structure
MIDAVKLHRPAARRRAGLFLAEGPNLVEAALRSGAVERIFATDAAIERFVDLLAEAPVRR
CCCCHHCCCCHHHHHHCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHH
VTERAAKALSDTVTPVGLVAQCRTVVSTWSQVTARSPRFVIVAVDISEPGNAGTLIRVAD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEC
AMGADAVVLAGNSVDPFNGKSLRASAGSIFNIPVVPEGDVGAIAADLAQTGVALLATTLD
CCCCCEEEEECCCCCCCCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEEECC
GDLSLDEADAVLAGPCAWFFGNEAHGLDGATAALATHRINIPMRGGAESLNLASAASICL
CCCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHH
YATARMHRRQ
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]