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The map label for this gene is tcmP [H]

Identifier: 169629255

GI number: 169629255

Start: 2183974

End: 2184798

Strand: Reverse

Name: tcmP [H]

Synonym: MAB_2169c

Alternate gene names: 169629255

Gene position: 2184798-2183974 (Counterclockwise)

Preceding gene: 169629266

Following gene: 169629247

Centisome position: 43.12

GC content: 61.33

Gene sequence:

>825_bases
ATGAGCCATTCCGAGAAGATTCATGTCGATCTCAGCGGCGCGCCGCAGACCATGCTGGCGACCTTCTACGCCAAGGCACT
CGACGCCGGCCTGCCCGAACCCATCCTCGGCGACCAGTTGGCCCGCGACATCGCGCAACGCATCGACTACGACTGGTCCA
AAACCACGATCACCGCCGGCAGATCCCCCGCCGTCACCACACGCTCGGCGCATTTCGACGAATGGTGCCGCGCCTTTTTG
TCCAGACACGCCGAGGCTGTTGTGCTGCATCTGGGTTGCGGTCTCGACGGCCGATACTTCCGCCTGAATCCCGGTCCCGG
GATCGAGTGGTACGACATCGACTATCCCGATGTGGCCAGGCTACGCAGCCAGCTTTATCCGACGCGTGATCACTACCACG
TCGTGTCGGCGTCGGTGACCGATCCGGCGTGGCTCGCGAACATCGCCGATAGCCGCCCGGTGCTGGCGATCGGCGAAGGG
CTCACGATGTACCTGAAGGAAGATGACGGCATCGCACTCTTTCGCCGGATCGTCGACCACTTTCCTTACGGGGAATTGCA
ATTCGACGCCTTCAACAAGTTCGGCATCAAGACACAGTGGTCCAACGCGGTGGTCCGGCGCTCAGGTGCGACGCTGTATT
GGGGCATAGACCGCCCCGAGGACCTCATTCGCGCCATACCGAAATTGCAATTGCTGGAATGGGAGTCGCCGTTCGACTCG
GAAACCTTCCAACGAACCAAGGCAGGCTACCGGCTGCTGGCCAGGGCGATGTCGCTGTCACCAACCCTTCGGTACATGGC
GCAATACCACCGCTACGCATTTTGA

Upstream 100 bases:

>100_bases
CGTTGCATGGTGTTCAGCCTAGAGAGGCGACGCTGAACACCACGCCGGGGACCGTTCAGAGTCCTGCGCGTGCCCCGAAA
ACGACATTAGCGTCGCGTCC

Downstream 100 bases:

>100_bases
GGAGGGAGGGTTGACCGCGACGGTCCTTGCCGTTTCGGGGTCGGCAATCGGGGGGACGGATCGCCGCGGCCAACCTGTCT
ATGACGCAGGTCTGCTCATC

Product: O-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSHSEKIHVDLSGAPQTMLATFYAKALDAGLPEPILGDQLARDIAQRIDYDWSKTTITAGRSPAVTTRSAHFDEWCRAFL
SRHAEAVVLHLGCGLDGRYFRLNPGPGIEWYDIDYPDVARLRSQLYPTRDHYHVVSASVTDPAWLANIADSRPVLAIGEG
LTMYLKEDDGIALFRRIVDHFPYGELQFDAFNKFGIKTQWSNAVVRRSGATLYWGIDRPEDLIRAIPKLQLLEWESPFDS
ETFQRTKAGYRLLARAMSLSPTLRYMAQYHRYAF

Sequences:

>Translated_274_residues
MSHSEKIHVDLSGAPQTMLATFYAKALDAGLPEPILGDQLARDIAQRIDYDWSKTTITAGRSPAVTTRSAHFDEWCRAFL
SRHAEAVVLHLGCGLDGRYFRLNPGPGIEWYDIDYPDVARLRSQLYPTRDHYHVVSASVTDPAWLANIADSRPVLAIGEG
LTMYLKEDDGIALFRRIVDHFPYGELQFDAFNKFGIKTQWSNAVVRRSGATLYWGIDRPEDLIRAIPKLQLLEWESPFDS
ETFQRTKAGYRLLARAMSLSPTLRYMAQYHRYAF
>Mature_273_residues
SHSEKIHVDLSGAPQTMLATFYAKALDAGLPEPILGDQLARDIAQRIDYDWSKTTITAGRSPAVTTRSAHFDEWCRAFLS
RHAEAVVLHLGCGLDGRYFRLNPGPGIEWYDIDYPDVARLRSQLYPTRDHYHVVSASVTDPAWLANIADSRPVLAIGEGL
TMYLKEDDGIALFRRIVDHFPYGELQFDAFNKFGIKTQWSNAVVRRSGATLYWGIDRPEDLIRAIPKLQLLEWESPFDSE
TFQRTKAGYRLLARAMSLSPTLRYMAQYHRYAF

Specific function: O-methyltransferase that catalyzes the methylation of the C-9 carboxy group of tetracenomycin E (TCM E) to yield TCM A2. Catalyzes as well the following side reactions:methylation of 8- O-methyl-TCM D3 to 9-carboxymethyl-8-O-methyl-TCM D3; and of TCM B3 t

COG id: COG3315

COG function: function code Q; O-Methyltransferase involved in polyketide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007213
- InterPro:   IPR016874 [H]

Pfam domain/function: PF04072 LCM [H]

EC number: NA

Molecular weight: Translated: 31203; Mature: 31071

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSEKIHVDLSGAPQTMLATFYAKALDAGLPEPILGDQLARDIAQRIDYDWSKTTITAG
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECC
RSPAVTTRSAHFDEWCRAFLSRHAEAVVLHLGCGLDGRYFRLNPGPGIEWYDIDYPDVAR
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCEEEECCCHHHHH
LRSQLYPTRDHYHVVSASVTDPAWLANIADSRPVLAIGEGLTMYLKEDDGIALFRRIVDH
HHHHCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHH
FPYGELQFDAFNKFGIKTQWSNAVVRRSGATLYWGIDRPEDLIRAIPKLQLLEWESPFDS
CCCCCEEECCHHCCCCEECCCCHHHCCCCCEEEECCCCHHHHHHHCCCCEEEECCCCCCH
ETFQRTKAGYRLLARAMSLSPTLRYMAQYHRYAF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHSEKIHVDLSGAPQTMLATFYAKALDAGLPEPILGDQLARDIAQRIDYDWSKTTITAG
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECC
RSPAVTTRSAHFDEWCRAFLSRHAEAVVLHLGCGLDGRYFRLNPGPGIEWYDIDYPDVAR
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCEEEECCCHHHHH
LRSQLYPTRDHYHVVSASVTDPAWLANIADSRPVLAIGEGLTMYLKEDDGIALFRRIVDH
HHHHCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHH
FPYGELQFDAFNKFGIKTQWSNAVVRRSGATLYWGIDRPEDLIRAIPKLQLLEWESPFDS
CCCCCEEECCHHCCCCEECCCCHHHCCCCCEEEECCCCHHHHHHHCCCCEEEECCCCCCH
ETFQRTKAGYRLLARAMSLSPTLRYMAQYHRYAF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]