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The map label for this gene is tcmL [H]

Identifier: 169629118

GI number: 169629118

Start: 2030675

End: 2031931

Strand: Direct

Name: tcmL [H]

Synonym: MAB_2031

Alternate gene names: 169629118

Gene position: 2030675-2031931 (Clockwise)

Preceding gene: 169629117

Following gene: 169629119

Centisome position: 40.08

GC content: 62.61

Gene sequence:

>1257_bases
GTGACAGGAACCGGCGTGGTGCTGCCGACGGGTCGTCACATCACACCCCTGTGGGACGCCTGGACTGACGGGAAACCGGC
TATCTCGCCGTACCTCGATCCGCTGGTCTCCACGCGCCGGATCACGCATTTCGGCCATATACCCGCCGAGGTCGTGGAGC
AAAGCCGTGAAGCAGTGCCACACAGACAGCGGAAATACGGCACCCCATACACCTTCAACGCGGTGTTGGCAGCTCAAGAT
GCGATCGCCGAGGCTGGACCTGGCTGGGCGAGGGTCGAGGACCGTCGACGAGGACTATTTGTCGCACAAGACAATTCCAC
TACTCCCAGCATCGGAACCTTCGCTCGAGGACTGACGCAACTGGGTGAGGGCCGCGAAATCGATTTCGGCGCATTGACAA
ACGAGCTACTCATGAACGGAGGGCTTGATCCGTTCACCATCATTCATGCCCTTAACAACAACACGCTCGCGCTGGTAAGC
ATCGTCCATGAGCTCCGCGGTGATTGCGCAGCGCTGGTCCAGGGCGAATCCGCGGCGGTCACCGCACTGCAACGTGCCAC
GTTCAGTCTGCGTAACGGGCATTGCGATATCGCACTGGTAGTGGGTGCGGGCAGCTACAACGAACCACTCACGCTGGCCG
GGCATTACCGCCTGGGCCATCTCAGCAAGGACGCTGGCGGCGATGGCCAGCTGCGGAGCTTCGATAGTGCGCAAGACGGA
ACGGTCCTGAGCGAGGGCGCGGTAGCGCTGGTGTTGGAACGCTCGCGAGACGCGACCGCGCGCGGTGCCACACCGCTGGT
CCAATTATGTGACACGGTTGTTGTTTCGGGCAGCCGCGGCGCCGGGCCCGCCCACATCGCTCAGCGTTACGACGACATGC
TGCACCAGAACGGGATCGGGACAGAGCAACTCGGCGCGGTGCTGGCCGACGGCAAGGCGGCCCCCGGCTACGACACCGCC
GAGATCGAACTGCTCACCACCTTGTTCAAGGGCAGTGCGATACCCGTATCGACCGTTCGACCGATAACCGGCACGCCGGG
AGCGGCCGGCCCGCTGGTCGATCTGGCACTCGCCGGGCAAATTTTCACCGAAAGTACTCTGCCGGCAATCACCCATCTCG
GGGATCCACTGTCTGATCAGCTCGAGTTCGTCCAGGGCGGACCCCGAAAGCAGCGGATCGACTCAGTTCTGGGCACCTAT
ACCAACTTCAACGGAATCTCCAGTGCGGTACTGGCCAAACGTCCAGACGCGGCGTGA

Upstream 100 bases:

>100_bases
ATCGAGGTGGCAATCAACAACTCGCTCGGGTTCGGTGGGCTCAACGCTGCTTTGGTGGTACGCCGTTATGACAACTGAAC
ATTCAGGTCAATCAATAGTC

Downstream 100 bases:

>100_bases
CCATCAGGACCACTTGAGCATCACAAACCAGGGAGAAACGTGTCTTTGAGCGGCAAAGAGTTTGAGGGTCAGGTCGCGCT
TGTCACCGGGGCCTCACGCG

Product: putative beta-ketoacyl synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTGTGVVLPTGRHITPLWDAWTDGKPAISPYLDPLVSTRRITHFGHIPAEVVEQSREAVPHRQRKYGTPYTFNAVLAAQD
AIAEAGPGWARVEDRRRGLFVAQDNSTTPSIGTFARGLTQLGEGREIDFGALTNELLMNGGLDPFTIIHALNNNTLALVS
IVHELRGDCAALVQGESAAVTALQRATFSLRNGHCDIALVVGAGSYNEPLTLAGHYRLGHLSKDAGGDGQLRSFDSAQDG
TVLSEGAVALVLERSRDATARGATPLVQLCDTVVVSGSRGAGPAHIAQRYDDMLHQNGIGTEQLGAVLADGKAAPGYDTA
EIELLTTLFKGSAIPVSTVRPITGTPGAAGPLVDLALAGQIFTESTLPAITHLGDPLSDQLEFVQGGPRKQRIDSVLGTY
TNFNGISSAVLAKRPDAA

Sequences:

>Translated_418_residues
MTGTGVVLPTGRHITPLWDAWTDGKPAISPYLDPLVSTRRITHFGHIPAEVVEQSREAVPHRQRKYGTPYTFNAVLAAQD
AIAEAGPGWARVEDRRRGLFVAQDNSTTPSIGTFARGLTQLGEGREIDFGALTNELLMNGGLDPFTIIHALNNNTLALVS
IVHELRGDCAALVQGESAAVTALQRATFSLRNGHCDIALVVGAGSYNEPLTLAGHYRLGHLSKDAGGDGQLRSFDSAQDG
TVLSEGAVALVLERSRDATARGATPLVQLCDTVVVSGSRGAGPAHIAQRYDDMLHQNGIGTEQLGAVLADGKAAPGYDTA
EIELLTTLFKGSAIPVSTVRPITGTPGAAGPLVDLALAGQIFTESTLPAITHLGDPLSDQLEFVQGGPRKQRIDSVLGTY
TNFNGISSAVLAKRPDAA
>Mature_417_residues
TGTGVVLPTGRHITPLWDAWTDGKPAISPYLDPLVSTRRITHFGHIPAEVVEQSREAVPHRQRKYGTPYTFNAVLAAQDA
IAEAGPGWARVEDRRRGLFVAQDNSTTPSIGTFARGLTQLGEGREIDFGALTNELLMNGGLDPFTIIHALNNNTLALVSI
VHELRGDCAALVQGESAAVTALQRATFSLRNGHCDIALVVGAGSYNEPLTLAGHYRLGHLSKDAGGDGQLRSFDSAQDGT
VLSEGAVALVLERSRDATARGATPLVQLCDTVVVSGSRGAGPAHIAQRYDDMLHQNGIGTEQLGAVLADGKAAPGYDTAE
IELLTTLFKGSAIPVSTVRPITGTPGAAGPLVDLALAGQIFTESTLPAITHLGDPLSDQLEFVQGGPRKQRIDSVLGTYT
NFNGISSAVLAKRPDAA

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Has A Preference For Short Chain Acid Substrates And May Function To Supply The Octanoic Substrates For Lipoic Acid Biosynthe

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Escherichia coli, GI1787337, Length=430, Percent_Identity=22.3255813953488, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71983541, Length=397, Percent_Identity=25.1889168765743, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 43945; Mature: 43814

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTGVVLPTGRHITPLWDAWTDGKPAISPYLDPLVSTRRITHFGHIPAEVVEQSREAVP
CCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
HRQRKYGTPYTFNAVLAAQDAIAEAGPGWARVEDRRRGLFVAQDNSTTPSIGTFARGLTQ
HHHHHCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHH
LGEGREIDFGALTNELLMNGGLDPFTIIHALNNNTLALVSIVHELRGDCAALVQGESAAV
HCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEHCCCCHHHH
TALQRATFSLRNGHCDIALVVGAGSYNEPLTLAGHYRLGHLSKDAGGDGQLRSFDSAQDG
HHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECEEECCCCCCCCCCCCCCCCCCCCCC
TVLSEGAVALVLERSRDATARGATPLVQLCDTVVVSGSRGAGPAHIAQRYDDMLHQNGIG
CEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCC
TEQLGAVLADGKAAPGYDTAEIELLTTLFKGSAIPVSTVRPITGTPGAAGPLVDLALAGQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IFTESTLPAITHLGDPLSDQLEFVQGGPRKQRIDSVLGTYTNFNGISSAVLAKRPDAA
HHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TGTGVVLPTGRHITPLWDAWTDGKPAISPYLDPLVSTRRITHFGHIPAEVVEQSREAVP
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
HRQRKYGTPYTFNAVLAAQDAIAEAGPGWARVEDRRRGLFVAQDNSTTPSIGTFARGLTQ
HHHHHCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHH
LGEGREIDFGALTNELLMNGGLDPFTIIHALNNNTLALVSIVHELRGDCAALVQGESAAV
HCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEHCCCCHHHH
TALQRATFSLRNGHCDIALVVGAGSYNEPLTLAGHYRLGHLSKDAGGDGQLRSFDSAQDG
HHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECEEECCCCCCCCCCCCCCCCCCCCCC
TVLSEGAVALVLERSRDATARGATPLVQLCDTVVVSGSRGAGPAHIAQRYDDMLHQNGIG
CEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCC
TEQLGAVLADGKAAPGYDTAEIELLTTLFKGSAIPVSTVRPITGTPGAAGPLVDLALAGQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IFTESTLPAITHLGDPLSDQLEFVQGGPRKQRIDSVLGTYTNFNGISSAVLAKRPDAA
HHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2684656; 1592819 [H]