Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 169629039

GI number: 169629039

Start: 1951383

End: 1952141

Strand: Reverse

Name: cobS [H]

Synonym: MAB_1952c

Alternate gene names: 169629039

Gene position: 1952141-1951383 (Counterclockwise)

Preceding gene: 169629040

Following gene: 169629038

Centisome position: 38.53

GC content: 70.49

Gene sequence:

>759_bases
GTGAATGCCCTGCTGCGAATCCTGCGGCCGGTGGGCGGCGCCTTCGAATTCGCCACCGTGGCACCGGTTCCGGCCCGGCT
GGCTGGCACGCTTTCCGGCCCGGTGCTGGGGGCGCTGCCGGTGGTGGGTGCAGCGCTGGGAGTCCTGGCCGCGTTCGTGG
CCTGGGTCATGCATTTCGCGTACATGGCGCCGATCGCCGGACTGGCAGCGGTCGCCGCGACAATCCTCGCAACCCGGGGC
CTACATATCGATGGGTTGTCCGACACCGCCGACGGGCTGGGCTGCTACGGGCCGCCGGAGCGCGCGCTGACCGTGATGCG
GGACGGCTCGGCCGGACCGTTCGGGGTGGTTGTGCTCATCATCTGCCTTCTAGCACAAGCATTGTCGTTCGGCGTACTGG
CCTCCCGGCAGGAGTGGCTGGCCGTCGCCCTGGCGATAACGGTGGGGCGAATCAGCGCGGTGGTGGCCTGTCGCCGCGGC
GTGCCCGGCGCCGCGGCGAGCGGATTCGGGACTAGGGTCGCCGGATCTCAACCGCTGTGGCTGGCGGGCATGTGGGTGGC
GGCGGCGATGGCCGTGGGCCTGCTGGCACAAACCTGGCGTCCCTGGCAGGGACCGGTGGTGGTGGCGGTGGCGCTGATCG
CCGGGGCGCTGCTGGTACGTCATTGTGTGCGCAGGTTCGGCGGAATCACCGGCGACGTCTTGGGCGCGAGCATCGAGATA
ACCACGACCGTGGCGGCCCTGGGTTTCGCGGCACACTGA

Upstream 100 bases:

>100_bases
GCGAGGGCTCCGGCGCGCTGGTGGCGCTGCCGGTGCTACAGGCCGCGGTCGGCACGCTCGCTCAGATGGCGACCTTCGAT
GAGGCCCGGGTGGACACCGC

Downstream 100 bases:

>100_bases
TGCGGCCAGGAAAGATTCATCAGGAGTTCGCGGTTGCATCGGCCTGTGCGGTCGCGTTACCGGCCGCCGTGGGCCACCAT
TCGAATCTATGTCGCGCAAT

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNALLRILRPVGGAFEFATVAPVPARLAGTLSGPVLGALPVVGAALGVLAAFVAWVMHFAYMAPIAGLAAVAATILATRG
LHIDGLSDTADGLGCYGPPERALTVMRDGSAGPFGVVVLIICLLAQALSFGVLASRQEWLAVALAITVGRISAVVACRRG
VPGAAASGFGTRVAGSQPLWLAGMWVAAAMAVGLLAQTWRPWQGPVVVAVALIAGALLVRHCVRRFGGITGDVLGASIEI
TTTVAALGFAAH

Sequences:

>Translated_252_residues
MNALLRILRPVGGAFEFATVAPVPARLAGTLSGPVLGALPVVGAALGVLAAFVAWVMHFAYMAPIAGLAAVAATILATRG
LHIDGLSDTADGLGCYGPPERALTVMRDGSAGPFGVVVLIICLLAQALSFGVLASRQEWLAVALAITVGRISAVVACRRG
VPGAAASGFGTRVAGSQPLWLAGMWVAAAMAVGLLAQTWRPWQGPVVVAVALIAGALLVRHCVRRFGGITGDVLGASIEI
TTTVAALGFAAH
>Mature_252_residues
MNALLRILRPVGGAFEFATVAPVPARLAGTLSGPVLGALPVVGAALGVLAAFVAWVMHFAYMAPIAGLAAVAATILATRG
LHIDGLSDTADGLGCYGPPERALTVMRDGSAGPFGVVVLIICLLAQALSFGVLASRQEWLAVALAITVGRISAVVACRRG
VPGAAASGFGTRVAGSQPLWLAGMWVAAAMAVGLLAQTWRPWQGPVVVAVALIAGALLVRHCVRRFGGITGDVLGASIEI
TTTVAALGFAAH

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=240, Percent_Identity=32.5, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 25403; Mature: 25403

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALLRILRPVGGAFEFATVAPVPARLAGTLSGPVLGALPVVGAALGVLAAFVAWVMHFA
CHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YMAPIAGLAAVAATILATRGLHIDGLSDTADGLGCYGPPERALTVMRDGSAGPFGVVVLI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCHHHHHHHH
ICLLAQALSFGVLASRQEWLAVALAITVGRISAVVACRRGVPGAAASGFGTRVAGSQPLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
LAGMWVAAAMAVGLLAQTWRPWQGPVVVAVALIAGALLVRHCVRRFGGITGDVLGASIEI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHH
TTTVAALGFAAH
HHHHHHHHHCCC
>Mature Secondary Structure
MNALLRILRPVGGAFEFATVAPVPARLAGTLSGPVLGALPVVGAALGVLAAFVAWVMHFA
CHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YMAPIAGLAAVAATILATRGLHIDGLSDTADGLGCYGPPERALTVMRDGSAGPFGVVVLI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCHHHHHHHH
ICLLAQALSFGVLASRQEWLAVALAITVGRISAVVACRRGVPGAAASGFGTRVAGSQPLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
LAGMWVAAAMAVGLLAQTWRPWQGPVVVAVALIAGALLVRHCVRRFGGITGDVLGASIEI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHH
TTTVAALGFAAH
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA