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The map label for this gene is lipA [H]

Identifier: 169629029

GI number: 169629029

Start: 1940669

End: 1941658

Strand: Reverse

Name: lipA [H]

Synonym: MAB_1942c

Alternate gene names: 169629029

Gene position: 1941658-1940669 (Counterclockwise)

Preceding gene: 169629030

Following gene: 169629028

Centisome position: 38.32

GC content: 64.95

Gene sequence:

>990_bases
ATGACAGAACCCCGCAAGCTACTGCGGCTGGAAGTCCGCAACGCCCAAACCCCGATCGAGCGCAAGCCGGACTGGATTCG
CACCCGCGCGAAGATGGGCCCCGAATACAAAGAACTCAAGTCGTTGGTGCGCTCCGGCGGACTGCACACCGTGTGCGAGG
AAGCCGGATGCCCCAACATCTTTGAGTGCTGGGAGGACCGTGAGGCCACCTTCCTCATCGGCGGTGAGCAATGCACACGC
CGCTGCGATTTCTGCCAGATCGACACTGGTAAGCCCGCCGACCTGGACCGCGACGAACCACGACGTGTGGCCGAGAGCGT
CCACACCATGGGCCTGCGGTACTCGACGGTTACCGGAGTGGCCCGCGACGACCTGCCCGACGGCGGTGCCTGGCTGTACG
CCGAAACGGTGCGATACATCAAGGACCTCAATCCGGCCACGGGGGTGGAGTTGCTCATCCCCGACTTCAACGCCATTCCC
GCGCAGCTGGACGAGGTATTCGCGTCCCGTCCGGAGGTGCTGGCGCACAACCTGGAGACGGTGCCACGGGTGTTCCGCCG
CATCCGGCCCGCGTTCAGCTATGAGCGAAGCCTGTCGGTGATCACCGCCGCGCGCGAGGCGCAGCTGGTGACCAAGAGCA
ATCTGATTCTCGGTATGGGCGAAACCAATGACGAGATTCGTGAAGCGCTGGTCGCCCTGCACGAAGCGGGCTGCGACATC
ATCACCATCACCCAGTATCTGCGGCCCTCGCCACGCCACCACCCGGTTGACCGGTGGGTCAAACCGCAGGAATTCGTGGA
GCTTTCGGAATTCGCGGAGGGGCTGGGCTTCGCCGGTGTGATGGCCGGCCCGCTGGTGCGCTCCTCCTACCGGGCCGGAC
GACTGTACGCACAGGCGATGGCTCATCATGGGCGTCCGCTGCCTGCCGCACTGGAACACCTGGCGACGGCGGGTTCGGCG
CGGCAAGAGGCCATCAACCTCATCGGATGA

Upstream 100 bases:

>100_bases
CCGAGCTGGGCCGCGACGTCACCGTCGATGAGGTGCACGACCAGGTTGTTTCCTCGGTTTTGGACGCGCTCGAAGGCCGG
CTTCCCGTCGGAGCGACGGC

Downstream 100 bases:

>100_bases
GAAGCGCGTCGCGGGCGGGCGATATTCTCTAGCCATGGCTAAATCACTGAGTCCCGCCGAGGCCAAGGCCGCCAAGGCCG
AGGCAAAGGCGCGCCGTAAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTEPRKLLRLEVRNAQTPIERKPDWIRTRAKMGPEYKELKSLVRSGGLHTVCEEAGCPNIFECWEDREATFLIGGEQCTR
RCDFCQIDTGKPADLDRDEPRRVAESVHTMGLRYSTVTGVARDDLPDGGAWLYAETVRYIKDLNPATGVELLIPDFNAIP
AQLDEVFASRPEVLAHNLETVPRVFRRIRPAFSYERSLSVITAAREAQLVTKSNLILGMGETNDEIREALVALHEAGCDI
ITITQYLRPSPRHHPVDRWVKPQEFVELSEFAEGLGFAGVMAGPLVRSSYRAGRLYAQAMAHHGRPLPAALEHLATAGSA
RQEAINLIG

Sequences:

>Translated_329_residues
MTEPRKLLRLEVRNAQTPIERKPDWIRTRAKMGPEYKELKSLVRSGGLHTVCEEAGCPNIFECWEDREATFLIGGEQCTR
RCDFCQIDTGKPADLDRDEPRRVAESVHTMGLRYSTVTGVARDDLPDGGAWLYAETVRYIKDLNPATGVELLIPDFNAIP
AQLDEVFASRPEVLAHNLETVPRVFRRIRPAFSYERSLSVITAAREAQLVTKSNLILGMGETNDEIREALVALHEAGCDI
ITITQYLRPSPRHHPVDRWVKPQEFVELSEFAEGLGFAGVMAGPLVRSSYRAGRLYAQAMAHHGRPLPAALEHLATAGSA
RQEAINLIG
>Mature_328_residues
TEPRKLLRLEVRNAQTPIERKPDWIRTRAKMGPEYKELKSLVRSGGLHTVCEEAGCPNIFECWEDREATFLIGGEQCTRR
CDFCQIDTGKPADLDRDEPRRVAESVHTMGLRYSTVTGVARDDLPDGGAWLYAETVRYIKDLNPATGVELLIPDFNAIPA
QLDEVFASRPEVLAHNLETVPRVFRRIRPAFSYERSLSVITAAREAQLVTKSNLILGMGETNDEIREALVALHEAGCDII
TITQYLRPSPRHHPVDRWVKPQEFVELSEFAEGLGFAGVMAGPLVRSSYRAGRLYAQAMAHHGRPLPAALEHLATAGSAR
QEAINLIG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=289, Percent_Identity=40.4844290657439, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=41.4634146341463, Blast_Score=197, Evalue=9e-51,
Organism=Escherichia coli, GI1786846, Length=302, Percent_Identity=42.0529801324503, Blast_Score=243, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=40.6976744186046, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324770, Length=285, Percent_Identity=42.1052631578947, Blast_Score=212, Evalue=7e-56,
Organism=Drosophila melanogaster, GI221513272, Length=289, Percent_Identity=41.8685121107266, Blast_Score=220, Evalue=9e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36751; Mature: 36620

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPRKLLRLEVRNAQTPIERKPDWIRTRAKMGPEYKELKSLVRSGGLHTVCEEAGCPNI
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCH
FECWEDREATFLIGGEQCTRRCDFCQIDTGKPADLDRDEPRRVAESVHTMGLRYSTVTGV
HHHHCCCCCEEEECCHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCC
ARDDLPDGGAWLYAETVRYIKDLNPATGVELLIPDFNAIPAQLDEVFASRPEVLAHNLET
HHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHH
VPRVFRRIRPAFSYERSLSVITAAREAQLVTKSNLILGMGETNDEIREALVALHEAGCDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHCCCCE
ITITQYLRPSPRHHPVDRWVKPQEFVELSEFAEGLGFAGVMAGPLVRSSYRAGRLYAQAM
EEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AHHGRPLPAALEHLATAGSARQEAINLIG
HHCCCCCHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
TEPRKLLRLEVRNAQTPIERKPDWIRTRAKMGPEYKELKSLVRSGGLHTVCEEAGCPNI
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCH
FECWEDREATFLIGGEQCTRRCDFCQIDTGKPADLDRDEPRRVAESVHTMGLRYSTVTGV
HHHHCCCCCEEEECCHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCC
ARDDLPDGGAWLYAETVRYIKDLNPATGVELLIPDFNAIPAQLDEVFASRPEVLAHNLET
HHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHH
VPRVFRRIRPAFSYERSLSVITAAREAQLVTKSNLILGMGETNDEIREALVALHEAGCDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHCCCCE
ITITQYLRPSPRHHPVDRWVKPQEFVELSEFAEGLGFAGVMAGPLVRSSYRAGRLYAQAM
EEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AHHGRPLPAALEHLATAGSARQEAINLIG
HHCCCCCHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA