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The map label for this gene is fadD26 [H]

Identifier: 169629003

GI number: 169629003

Start: 1913227

End: 1915029

Strand: Direct

Name: fadD26 [H]

Synonym: MAB_1915

Alternate gene names: 169629003

Gene position: 1913227-1915029 (Clockwise)

Preceding gene: 169629001

Following gene: 169629005

Centisome position: 37.76

GC content: 65.95

Gene sequence:

>1803_bases
ATGAAGTTGCGTGTTGAGGAGTACTTGGACGGCAAAGGTGCTATCACCTTGCCAGACGGTTATACCGTCAACTACTACCT
TGAGCGTGCCGTGGAAGAGCTGGGCGACACGTTCGCCTACCGGTATCTGGACTTCAACGCCAACTCTGACGGCGAACCCA
ATGATCTCAACTGGACGCAGCTTGGCCAGCGTTCTCGGGCCGTTGCCGCGCGGCTCCAGCAAGTAACCAAGCCCGGCGAT
CGGGTGGCCATTCTGGCCCCGCAGGGCATTGACTATGTGGTGGGTTTCTTTGCCGCCATCGAGGCCGGGAACATCGCGGT
TCCATTGTTTGCTCCGGAGCTTCCCGGCCACTCTGAACGGCTCGACGCGGTGCTCACCGACGCACAGCCGACGGTGGTGC
TGACCAATAACGCCGCCGCCGAGTCCGTCAGCCGTTTTGTCCGCGGTCTGCCGCGCGACCGCCGCCCCCGCGTGGTGGCG
GTGGACAGCGTGCCCGACTCGGTGGCCGCCACCTACGCCAAGGTGACGCCGGATACCGATGACATCGCCTATCTGCAATA
CACGTCGGGATCCACCCGGGTGCCCGCGGGCGTCGAGATCACGCACCGCGCGGTGATGACCAATGTTCTGCAGATGATCA
TCTCGGTCGGTCTGGACGACAGCATCCGCAGCGTCAGCTGGCTGCCGCTGTACCACGACATGGGCCTGCTGATGATTCTG
TTCCCGCTGTGCGGTGGCCGGATCACCCTGATGTCCCCGGTGTCGTTCGTGCGACGGCCCGGGCGGTGGATCAAGGAGCT
CGCCGCCGAGGGGCATCTGGGCCGAACCTTTGCCGCCGCACCGAACTTCGCATTCGAACTGGCCGCGGAGCGGGGTCTGC
CCAAGGACGGCGAAGAGCTGGACCTGAGCAACGTCGCGGGCCTGATCAACGGTTCCGAGCCCGTCAGCATCTCCTCGATC
CGCAAGTTCAATGACGCTTTCGGGCCGTACGGGCTGCCGCCGACCACCATCAAGCCGTCCTATGGAATGGCCGAGGCGAC
GTTGTTCGTCTCCACCATCCCCTCCGACGCCGAGGCGTCGGTGGTGTATCTGGACCGCCGGGCACTGGGCGACGGACACG
CGGTGCGTGTTGAGGCCGACGACGAGAACGCGGTGCCGCAGGTGTCTTGCGGGAAGATCGCGCGCAGTCAGTGGGCGGTG
ATCGTCAACCCGAACGCCGATACCGAGCTGGCCGACGGCGAGGTTGGGGAGATCTGGCTGCACGGCGACAACATCGGCCG
CGGTTACTGGGGCAGGCCCAAGGAGACGGACTTCTCGTTCCGCAACAAGCTGCAGGCACGGCTGGATCACGGCAGCCACG
CCACCGGTACCGAGCCCGGCGCCACGTGGTTCCGCACCGGCGATCTGGGCGTGTACCTCGATGGTGAGCTCTACATCACC
GGGCGGGTCAAGGACCTGGTCATCATCGACGGTCGCAACCACTACCCGCAGGACATCGAGGCCACCGTCGAAGAGGCCTC
GCCCGCGGTGCGCCGTGGTTTCGTGGCGGCATTCTCGGTGCCCGCCGAAGGATCTGGGGAGCAGCTGGTCATCGTGGCAG
AGCGCGCCGCCGGTGCGGGCCGGGCCGAGCCGGAGCCCATCATCGATGCGATCCGCGCCGCCGTCTCGCGACGCCACAAC
CTGCCGATCGCGGATGTGCAGCTGGTACAGGCGGGTGCGATTCCCCGCACTACCAGCGGAAAGCTCGCACGCCGAGCCTG
CCGCCAGGAGTACCTGGACAACAAACTCGGCGTGCGCGCCTGA

Upstream 100 bases:

>100_bases
CGCTTCGCACCGCGCGTGGTGCCGAGTGTGAGTGTTGCCTGGTCGGTATTGTCCCCCTTATGGGACGCCAGTCGTGTTCA
CAGCTAGACCGAGGTCACCC

Downstream 100 bases:

>100_bases
CGATCTCGATTTAGTACGAGTGCTCGGGAGCGGGGAACACTCCGCGAGCCACCTCATCGGCGTACTGCTCGGCGGCCTTG
CGTAGCTCGGCACCGACCTG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKLRVEEYLDGKGAITLPDGYTVNYYLERAVEELGDTFAYRYLDFNANSDGEPNDLNWTQLGQRSRAVAARLQQVTKPGD
RVAILAPQGIDYVVGFFAAIEAGNIAVPLFAPELPGHSERLDAVLTDAQPTVVLTNNAAAESVSRFVRGLPRDRRPRVVA
VDSVPDSVAATYAKVTPDTDDIAYLQYTSGSTRVPAGVEITHRAVMTNVLQMIISVGLDDSIRSVSWLPLYHDMGLLMIL
FPLCGGRITLMSPVSFVRRPGRWIKELAAEGHLGRTFAAAPNFAFELAAERGLPKDGEELDLSNVAGLINGSEPVSISSI
RKFNDAFGPYGLPPTTIKPSYGMAEATLFVSTIPSDAEASVVYLDRRALGDGHAVRVEADDENAVPQVSCGKIARSQWAV
IVNPNADTELADGEVGEIWLHGDNIGRGYWGRPKETDFSFRNKLQARLDHGSHATGTEPGATWFRTGDLGVYLDGELYIT
GRVKDLVIIDGRNHYPQDIEATVEEASPAVRRGFVAAFSVPAEGSGEQLVIVAERAAGAGRAEPEPIIDAIRAAVSRRHN
LPIADVQLVQAGAIPRTTSGKLARRACRQEYLDNKLGVRA

Sequences:

>Translated_600_residues
MKLRVEEYLDGKGAITLPDGYTVNYYLERAVEELGDTFAYRYLDFNANSDGEPNDLNWTQLGQRSRAVAARLQQVTKPGD
RVAILAPQGIDYVVGFFAAIEAGNIAVPLFAPELPGHSERLDAVLTDAQPTVVLTNNAAAESVSRFVRGLPRDRRPRVVA
VDSVPDSVAATYAKVTPDTDDIAYLQYTSGSTRVPAGVEITHRAVMTNVLQMIISVGLDDSIRSVSWLPLYHDMGLLMIL
FPLCGGRITLMSPVSFVRRPGRWIKELAAEGHLGRTFAAAPNFAFELAAERGLPKDGEELDLSNVAGLINGSEPVSISSI
RKFNDAFGPYGLPPTTIKPSYGMAEATLFVSTIPSDAEASVVYLDRRALGDGHAVRVEADDENAVPQVSCGKIARSQWAV
IVNPNADTELADGEVGEIWLHGDNIGRGYWGRPKETDFSFRNKLQARLDHGSHATGTEPGATWFRTGDLGVYLDGELYIT
GRVKDLVIIDGRNHYPQDIEATVEEASPAVRRGFVAAFSVPAEGSGEQLVIVAERAAGAGRAEPEPIIDAIRAAVSRRHN
LPIADVQLVQAGAIPRTTSGKLARRACRQEYLDNKLGVRA
>Mature_600_residues
MKLRVEEYLDGKGAITLPDGYTVNYYLERAVEELGDTFAYRYLDFNANSDGEPNDLNWTQLGQRSRAVAARLQQVTKPGD
RVAILAPQGIDYVVGFFAAIEAGNIAVPLFAPELPGHSERLDAVLTDAQPTVVLTNNAAAESVSRFVRGLPRDRRPRVVA
VDSVPDSVAATYAKVTPDTDDIAYLQYTSGSTRVPAGVEITHRAVMTNVLQMIISVGLDDSIRSVSWLPLYHDMGLLMIL
FPLCGGRITLMSPVSFVRRPGRWIKELAAEGHLGRTFAAAPNFAFELAAERGLPKDGEELDLSNVAGLINGSEPVSISSI
RKFNDAFGPYGLPPTTIKPSYGMAEATLFVSTIPSDAEASVVYLDRRALGDGHAVRVEADDENAVPQVSCGKIARSQWAV
IVNPNADTELADGEVGEIWLHGDNIGRGYWGRPKETDFSFRNKLQARLDHGSHATGTEPGATWFRTGDLGVYLDGELYIT
GRVKDLVIIDGRNHYPQDIEATVEEASPAVRRGFVAAFSVPAEGSGEQLVIVAERAAGAGRAEPEPIIDAIRAAVSRRHN
LPIADVQLVQAGAIPRTTSGKLARRACRQEYLDNKLGVRA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=578, Percent_Identity=25.2595155709343, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI55749758, Length=620, Percent_Identity=24.3548387096774, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI225735629, Length=478, Percent_Identity=24.2677824267782, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI45827692, Length=478, Percent_Identity=24.2677824267782, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI187761345, Length=534, Percent_Identity=26.0299625468165, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI187761343, Length=534, Percent_Identity=26.0299625468165, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788107, Length=583, Percent_Identity=27.958833619211, Blast_Score=128, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=533, Percent_Identity=25.140712945591, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=543, Percent_Identity=25.0460405156538, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193202679, Length=636, Percent_Identity=22.9559748427673, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=583, Percent_Identity=23.4991423670669, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24654656, Length=435, Percent_Identity=24.1379310344828, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 65001; Mature: 65001

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRVEEYLDGKGAITLPDGYTVNYYLERAVEELGDTFAYRYLDFNANSDGEPNDLNWTQ
CCEEHHHHCCCCCEEECCCCCEEHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHH
LGQRSRAVAARLQQVTKPGDRVAILAPQGIDYVVGFFAAIEAGNIAVPLFAPELPGHSER
HHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
LDAVLTDAQPTVVLTNNAAAESVSRFVRGLPRDRRPRVVAVDSVPDSVAATYAKVTPDTD
HHHEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHEEEECCCCC
DIAYLQYTSGSTRVPAGVEITHRAVMTNVLQMIISVGLDDSIRSVSWLPLYHDMGLLMIL
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCEEEEEHHHHHHHHHHH
FPLCGGRITLMSPVSFVRRPGRWIKELAAEGHLGRTFAAAPNFAFELAAERGLPKDGEEL
HHHCCCEEEEECCHHHHHCCCHHHHHHHHCCCCCCEEECCCCHHEEHHHHCCCCCCCCCC
DLSNVAGLINGSEPVSISSIRKFNDAFGPYGLPPTTIKPSYGMAEATLFVSTIPSDAEAS
CHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCE
VVYLDRRALGDGHAVRVEADDENAVPQVSCGKIARSQWAVIVNPNADTELADGEVGEIWL
EEEEECHHCCCCCEEEEECCCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCEEEEE
HGDNIGRGYWGRPKETDFSFRNKLQARLDHGSHATGTEPGATWFRTGDLGVYLDGELYIT
ECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEECCEEEEE
GRVKDLVIIDGRNHYPQDIEATVEEASPAVRRGFVAAFSVPAEGSGEQLVIVAERAAGAG
ECEEEEEEECCCCCCCCHHHHHHHHCCHHHHHCEEEEEECCCCCCCCEEEEEEECCCCCC
RAEPEPIIDAIRAAVSRRHNLPIADVQLVQAGAIPRTTSGKLARRACRQEYLDNKLGVRA
CCCCHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLRVEEYLDGKGAITLPDGYTVNYYLERAVEELGDTFAYRYLDFNANSDGEPNDLNWTQ
CCEEHHHHCCCCCEEECCCCCEEHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHH
LGQRSRAVAARLQQVTKPGDRVAILAPQGIDYVVGFFAAIEAGNIAVPLFAPELPGHSER
HHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
LDAVLTDAQPTVVLTNNAAAESVSRFVRGLPRDRRPRVVAVDSVPDSVAATYAKVTPDTD
HHHEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHEEEECCCCC
DIAYLQYTSGSTRVPAGVEITHRAVMTNVLQMIISVGLDDSIRSVSWLPLYHDMGLLMIL
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCEEEEEHHHHHHHHHHH
FPLCGGRITLMSPVSFVRRPGRWIKELAAEGHLGRTFAAAPNFAFELAAERGLPKDGEEL
HHHCCCEEEEECCHHHHHCCCHHHHHHHHCCCCCCEEECCCCHHEEHHHHCCCCCCCCCC
DLSNVAGLINGSEPVSISSIRKFNDAFGPYGLPPTTIKPSYGMAEATLFVSTIPSDAEAS
CHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCE
VVYLDRRALGDGHAVRVEADDENAVPQVSCGKIARSQWAVIVNPNADTELADGEVGEIWL
EEEEECHHCCCCCEEEEECCCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCEEEEE
HGDNIGRGYWGRPKETDFSFRNKLQARLDHGSHATGTEPGATWFRTGDLGVYLDGELYIT
ECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEECCEEEEE
GRVKDLVIIDGRNHYPQDIEATVEEASPAVRRGFVAAFSVPAEGSGEQLVIVAERAAGAG
ECEEEEEEECCCCCCCCHHHHHHHHCCHHHHHCEEEEEECCCCCCCCEEEEEEECCCCCC
RAEPEPIIDAIRAAVSRRHNLPIADVQLVQAGAIPRTTSGKLARRACRQEYLDNKLGVRA
CCCCHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]