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The map label for this gene is amiA2 [H]

Identifier: 169628764

GI number: 169628764

Start: 1701565

End: 1702965

Strand: Reverse

Name: amiA2 [H]

Synonym: MAB_1674c

Alternate gene names: 169628764

Gene position: 1702965-1701565 (Counterclockwise)

Preceding gene: 169628768

Following gene: 169628754

Centisome position: 33.61

GC content: 69.38

Gene sequence:

>1401_bases
ATGTCGGGCTTTCCCACGCTGACTTCCCAGGCCGCAGCGCTATCTGACGGTTCGGAGAGTTCGGTCTCGCTCACCCTCAC
GGCGCTCAACGCGATCAAGTCGAGCCAGTCGACCCTCAACGCATTCCGGGTGGTGTGCACCAAGACGGCCCTGGCCGAAG
CCACCGAGGCCGACAAGCGATTAGCCAGGGGTGAGCGCCTGCCGTTGCTCGGAGTGCCCATCGCCATCAAGGACGACACC
GACTTGGCGGGCACTCCCACCGCATTCGGTACCGCCGGGGCCGTCGGCTCGGAGTCCGAGGACGCCGAGCTGGTGCGCCG
ATTGCGCGCGGCGGGCGCGGTGATCGTCGGCAAGACCAACACCTGCGAGCTGGGCCAGTGGGGATTCACCAGCGGGCCCG
GCTTCGGGCACACCCGCAACCCCTGGAGCCGCGGCCACACCCCGGGCGGATCCTCAGGTGGAAGCGCCGCGGCGGTGGCG
GCCGGGCTCGTCGCCGGCGCCATCGGTTCGGACGGGGCCGGAAGCGTCCGGATCCCGGCGGCCTGGACGCACCTGATCGG
TATCAAGCCGCAACGCGGCCGCATCTCCACCTGGCCGTTTGCCGAGGCCTTCAACGGGATAACGGTGCACGGACCGTTGG
CCCGCACGGTCGCCGATGCCGCACTGCTGCTGGACGCGGCATCGGGAAACGTCCCCGGGGACTTGCACAAGCCGCCGCAC
GTCCGGGTGCTCGACGCCGTCCACGAGGACCCGGGACAACTGCGCGTCGCGTTATCGCTCAAGGTTCCGTTCTCGGGTTT
CCCGGCGCAACTGCATCCGACCATCGAGGCCGCCACCCGCTATGTGGCCCACCAGCTGCGTTCACTGGGGCACATGGTGT
CCGAGGGCGACCCGGACTATGGCGTGGGGCTCGGCCTGAACTTTCTGCCGCGAGCGACGGCCGGGCTGCTGCCGTGGCGC
GATCGGCTGGACGCCGGAGCGCATTGGGACCCGCGCACGGTGACGAATATGCGCACCGGCCGGCGCCTAGCCGGCTGGGC
GCTGCGGCGAGCCCGCGCCGCCGAGCCGCGGCTGCAGGCCCGAGTGGGCAAGATCTTCGGCAGCTTCAATGTGGTGTTGG
CACCGACAACCGCCCAACCGCCCACGGGCATTTACGATTTCGATGATGTCGGCAGCCTCGCCACGGATCGCGGGCAGGTC
GGCGCGTGTCCGATGACGTGGCCATGGAACGTGCTGGGCTGGCCGTCGATCAATGTGCCCGCCGGCTTCACGGCCGACGG
GCTGCCGATCGGTGTGCAGTTGATGGGCCCGGCGAACAGCGAGCCGCTGCTGATTTCCCTTGCCGCGCAGCTGGAGTCGA
TCAACAATTGGGCGTCGGAGATCCCCGATCCCTGGTGGTGA

Upstream 100 bases:

>100_bases
GACAGCACGATCCCGATAAAGCCGCATTCGGATAGTTTCCCATCTGGTACCGACGAATCCCCGCATTGACGCACTATTTC
GACCGCTTACCCTTCTGGTC

Downstream 100 bases:

>100_bases
GAGGCGCGCGCCTACGGCGCCGCCTCTGCGCCGCCTCTGCGCCGACACGCCGTATTGTGCGTCGGCCACTCGAGATATGC
ATCAGAAACCGTAGTTTCGA

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSGFPTLTSQAAALSDGSESSVSLTLTALNAIKSSQSTLNAFRVVCTKTALAEATEADKRLARGERLPLLGVPIAIKDDT
DLAGTPTAFGTAGAVGSESEDAELVRRLRAAGAVIVGKTNTCELGQWGFTSGPGFGHTRNPWSRGHTPGGSSGGSAAAVA
AGLVAGAIGSDGAGSVRIPAAWTHLIGIKPQRGRISTWPFAEAFNGITVHGPLARTVADAALLLDAASGNVPGDLHKPPH
VRVLDAVHEDPGQLRVALSLKVPFSGFPAQLHPTIEAATRYVAHQLRSLGHMVSEGDPDYGVGLGLNFLPRATAGLLPWR
DRLDAGAHWDPRTVTNMRTGRRLAGWALRRARAAEPRLQARVGKIFGSFNVVLAPTTAQPPTGIYDFDDVGSLATDRGQV
GACPMTWPWNVLGWPSINVPAGFTADGLPIGVQLMGPANSEPLLISLAAQLESINNWASEIPDPWW

Sequences:

>Translated_466_residues
MSGFPTLTSQAAALSDGSESSVSLTLTALNAIKSSQSTLNAFRVVCTKTALAEATEADKRLARGERLPLLGVPIAIKDDT
DLAGTPTAFGTAGAVGSESEDAELVRRLRAAGAVIVGKTNTCELGQWGFTSGPGFGHTRNPWSRGHTPGGSSGGSAAAVA
AGLVAGAIGSDGAGSVRIPAAWTHLIGIKPQRGRISTWPFAEAFNGITVHGPLARTVADAALLLDAASGNVPGDLHKPPH
VRVLDAVHEDPGQLRVALSLKVPFSGFPAQLHPTIEAATRYVAHQLRSLGHMVSEGDPDYGVGLGLNFLPRATAGLLPWR
DRLDAGAHWDPRTVTNMRTGRRLAGWALRRARAAEPRLQARVGKIFGSFNVVLAPTTAQPPTGIYDFDDVGSLATDRGQV
GACPMTWPWNVLGWPSINVPAGFTADGLPIGVQLMGPANSEPLLISLAAQLESINNWASEIPDPWW
>Mature_465_residues
SGFPTLTSQAAALSDGSESSVSLTLTALNAIKSSQSTLNAFRVVCTKTALAEATEADKRLARGERLPLLGVPIAIKDDTD
LAGTPTAFGTAGAVGSESEDAELVRRLRAAGAVIVGKTNTCELGQWGFTSGPGFGHTRNPWSRGHTPGGSSGGSAAAVAA
GLVAGAIGSDGAGSVRIPAAWTHLIGIKPQRGRISTWPFAEAFNGITVHGPLARTVADAALLLDAASGNVPGDLHKPPHV
RVLDAVHEDPGQLRVALSLKVPFSGFPAQLHPTIEAATRYVAHQLRSLGHMVSEGDPDYGVGLGLNFLPRATAGLLPWRD
RLDAGAHWDPRTVTNMRTGRRLAGWALRRARAAEPRLQARVGKIFGSFNVVLAPTTAQPPTGIYDFDDVGSLATDRGQVG
ACPMTWPWNVLGWPSINVPAGFTADGLPIGVQLMGPANSEPLLISLAAQLESINNWASEIPDPWW

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=502, Percent_Identity=27.0916334661355, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=25.3578732106339, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=25.0996015936255, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=303, Percent_Identity=29.7029702970297, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=427, Percent_Identity=25.7611241217799, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=264, Percent_Identity=28.7878787878788, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=222, Percent_Identity=30.1801801801802, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=33.8541666666667, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=33.8541666666667, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=445, Percent_Identity=27.1910112359551, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=167, Percent_Identity=31.7365269461078, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24644968, Length=494, Percent_Identity=22.6720647773279, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356731, Length=244, Percent_Identity=31.5573770491803, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652985, Length=500, Percent_Identity=26, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922090, Length=500, Percent_Identity=26, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24652981, Length=500, Percent_Identity=26, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24652983, Length=500, Percent_Identity=26, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161078093, Length=489, Percent_Identity=26.5848670756646, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI45550774, Length=202, Percent_Identity=31.6831683168317, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648435, Length=202, Percent_Identity=31.6831683168317, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648437, Length=202, Percent_Identity=31.6831683168317, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648113, Length=230, Percent_Identity=37.3913043478261, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648441, Length=171, Percent_Identity=32.7485380116959, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648439, Length=171, Percent_Identity=32.7485380116959, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 48674; Mature: 48542

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFPTLTSQAAALSDGSESSVSLTLTALNAIKSSQSTLNAFRVVCTKTALAEATEADKR
CCCCCCCHHHHHHCCCCCCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LARGERLPLLGVPIAIKDDTDLAGTPTAFGTAGAVGSESEDAELVRRLRAAGAVIVGKTN
HHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCC
TCELGQWGFTSGPGFGHTRNPWSRGHTPGGSSGGSAAAVAAGLVAGAIGSDGAGSVRIPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCH
AWTHLIGIKPQRGRISTWPFAEAFNGITVHGPLARTVADAALLLDAASGNVPGDLHKPPH
HHHHHEECCCCCCCCCCCCHHHHCCCEEEECHHHHHHHHHHHEEECCCCCCCCCCCCCCC
VRVLDAVHEDPGQLRVALSLKVPFSGFPAQLHPTIEAATRYVAHQLRSLGHMVSEGDPDY
EEEEHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVGLGLNFLPRATAGLLPWRDRLDAGAHWDPRTVTNMRTGRRLAGWALRRARAAEPRLQA
CCCCCCCCCCHHHHCCCCCHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHH
RVGKIFGSFNVVLAPTTAQPPTGIYDFDDVGSLATDRGQVGACPMTWPWNVLGWPSINVP
HHHHHHCCCEEEEECCCCCCCCCCCCCHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCC
AGFTADGLPIGVQLMGPANSEPLLISLAAQLESINNWASEIPDPWW
CCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGFPTLTSQAAALSDGSESSVSLTLTALNAIKSSQSTLNAFRVVCTKTALAEATEADKR
CCCCCCHHHHHHCCCCCCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LARGERLPLLGVPIAIKDDTDLAGTPTAFGTAGAVGSESEDAELVRRLRAAGAVIVGKTN
HHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCC
TCELGQWGFTSGPGFGHTRNPWSRGHTPGGSSGGSAAAVAAGLVAGAIGSDGAGSVRIPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCH
AWTHLIGIKPQRGRISTWPFAEAFNGITVHGPLARTVADAALLLDAASGNVPGDLHKPPH
HHHHHEECCCCCCCCCCCCHHHHCCCEEEECHHHHHHHHHHHEEECCCCCCCCCCCCCCC
VRVLDAVHEDPGQLRVALSLKVPFSGFPAQLHPTIEAATRYVAHQLRSLGHMVSEGDPDY
EEEEHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVGLGLNFLPRATAGLLPWRDRLDAGAHWDPRTVTNMRTGRRLAGWALRRARAAEPRLQA
CCCCCCCCCCHHHHCCCCCHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHH
RVGKIFGSFNVVLAPTTAQPPTGIYDFDDVGSLATDRGQVGACPMTWPWNVLGWPSINVP
HHHHHHCCCEEEEECCCCCCCCCCCCCHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCC
AGFTADGLPIGVQLMGPANSEPLLISLAAQLESINNWASEIPDPWW
CCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]