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The map label for this gene is tig

Identifier: 169628670

GI number: 169628670

Start: 1609681

End: 1611153

Strand: Direct

Name: tig

Synonym: MAB_1580

Alternate gene names: 169628670

Gene position: 1609681-1611153 (Clockwise)

Preceding gene: 169628669

Following gene: 169628671

Centisome position: 31.77

GC content: 66.4

Gene sequence:

>1473_bases
GTGAAGAGCACCGTCGAAAAGCTGAGCCCGACCCGGGTTCGCATCAACGTCGAGGTTCCCTTCGCAGAGCTCGAACCAGA
TTTCTCCAAGGCCTACAAGGAGCTGGCGCAGCAGGTTCGCCTGCCCGGTTTCCGCCCCGGCAAGGCTCCCGCCAAGCTGC
TTGAGGCCCGCGTCGGCCGTGCCGCCGTGCTGGAGCAGGTCGTCAACGCGGCCCTGCCGGCCCGCTACAGCGAGGCCGTC
ACCGCCTCCGACGTCAAGCCGCTGGGCCAGCCGGAGATCGAGGTCACCAAGATCGAGGACGGCCAGGAGCTGACATTCAC
CGCTGAGGTGGATGTGCGCCCCGAGATCGAGCTGCCCGACCTGAGCGGAGTTGCCGTCACCGTCAAGCCCGTCACCGTCG
AGGACTCCGAGGTCGAGGCCGAGCTGGACGCGCTGCGCGCCCGGTTCGGCACCCTTAAGGGCGTCGAGCGCGCCGCCGAG
AACGGCGACTTCGTCTCGATCGATCTGTCGGCCACCGTTGACGGCAAGGAGGTCGAGGAGGCGGCGACCACCGGTCTGTC
GCACGAGATCGGCTCCGGACAGCTGATCGACGGCCTGGATGAGGCCATCATCGGGCTGAAGGCCGGCGAGGAGAAGGTGT
TCACCACCAAGTTGGCCGCCGGCGAGTTCGCCGGCCAGGAGGCTGAGGTCACCGTCAAGGTCGGCTCCATCAAGGAGCGC
GAGCTGCCGGACGCCGACGACGACTTTGCACAGCTGGCAAGCGAATTCGACACCATCGGCGAGCTCAAGGACAGCCTCAC
CGAGCAGGTGAAGGGCCGCAAGCGCATCGCGCAGGCCGACGAGATCCGCGACGAGACCATCACCGCGCTGCTCGACAAGA
TCGAGATCCCGGTGCCGGAGAAGATCCTCGAGGAGCAGATCGGCAACAACCTGCATGAGGCCGTCCACGGCCTGGACCAC
AACGAGGAGCGTCTCAACGAGCTGCTTCAGGAGCAGGGCAGCTCGCGCGAGGAGTTCGACAAGGACATGCGTGAGTCCGC
GACCAAGTCCATCAAGACCGAGCTGCTGCTGGACGTGATCGCCGACAAGTTCGACATCAACGTCGACCAGCAGGACCTCA
CCGAGCGCCTGGTGCTCATGTCGCGCCAGTACGGCATCGAGCCACAGCAGCTGGTGCAGTACCTGACACAGCAGCAGCAG
CTGCCCGGCCTCTACGTCGACGTGCGTCGCGGCAAGGCCATCGCCGAGGTCATCCGGCAGGCCAAGGTGACCGACTCCAC
CGGCGCCGACGTGGACGTCGACGCCGTGCTGGGACCGCGCCGCGGCGGAGCCGACGAGGCCGGCGCTGAGGCAGAGGCGG
CCGAGGAGAAGCCGGCGAAGGCCAAGAAGAGCGCGGACAGCGAGAAAACAGACAAGTCCGAGAAGGCCGAGAAGAAGTCC
AAGAAGAAGAGCAAGGACGACGACGCCGAGTAG

Upstream 100 bases:

>100_bases
CGGAGGGTCGCAGGTTCAAATCCTGTCACCCCGACAGCACGACAGTATGAAAAAACGCAGGTCAGGATGGCCTGCCGCGA
ACTACAAAGGAGCACTGCAC

Downstream 100 bases:

>100_bases
TGCGCGTCACGCGGCGATGAGAGTCTCGCGCGAAGAGCATCACGCTCTGAGCGAACTCGCCCGTGGCGGGGAGTGCGCAG
CAGCCGATGTTGGTTAATGT

Product: trigger factor (TF)

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MKSTVEKLSPTRVRINVEVPFAELEPDFSKAYKELAQQVRLPGFRPGKAPAKLLEARVGRAAVLEQVVNAALPARYSEAV
TASDVKPLGQPEIEVTKIEDGQELTFTAEVDVRPEIELPDLSGVAVTVKPVTVEDSEVEAELDALRARFGTLKGVERAAE
NGDFVSIDLSATVDGKEVEEAATTGLSHEIGSGQLIDGLDEAIIGLKAGEEKVFTTKLAAGEFAGQEAEVTVKVGSIKER
ELPDADDDFAQLASEFDTIGELKDSLTEQVKGRKRIAQADEIRDETITALLDKIEIPVPEKILEEQIGNNLHEAVHGLDH
NEERLNELLQEQGSSREEFDKDMRESATKSIKTELLLDVIADKFDINVDQQDLTERLVLMSRQYGIEPQQLVQYLTQQQQ
LPGLYVDVRRGKAIAEVIRQAKVTDSTGADVDVDAVLGPRRGGADEAGAEAEAAEEKPAKAKKSADSEKTDKSEKAEKKS
KKKSKDDDAE

Sequences:

>Translated_490_residues
MKSTVEKLSPTRVRINVEVPFAELEPDFSKAYKELAQQVRLPGFRPGKAPAKLLEARVGRAAVLEQVVNAALPARYSEAV
TASDVKPLGQPEIEVTKIEDGQELTFTAEVDVRPEIELPDLSGVAVTVKPVTVEDSEVEAELDALRARFGTLKGVERAAE
NGDFVSIDLSATVDGKEVEEAATTGLSHEIGSGQLIDGLDEAIIGLKAGEEKVFTTKLAAGEFAGQEAEVTVKVGSIKER
ELPDADDDFAQLASEFDTIGELKDSLTEQVKGRKRIAQADEIRDETITALLDKIEIPVPEKILEEQIGNNLHEAVHGLDH
NEERLNELLQEQGSSREEFDKDMRESATKSIKTELLLDVIADKFDINVDQQDLTERLVLMSRQYGIEPQQLVQYLTQQQQ
LPGLYVDVRRGKAIAEVIRQAKVTDSTGADVDVDAVLGPRRGGADEAGAEAEAAEEKPAKAKKSADSEKTDKSEKAEKKS
KKKSKDDDAE
>Mature_490_residues
MKSTVEKLSPTRVRINVEVPFAELEPDFSKAYKELAQQVRLPGFRPGKAPAKLLEARVGRAAVLEQVVNAALPARYSEAV
TASDVKPLGQPEIEVTKIEDGQELTFTAEVDVRPEIELPDLSGVAVTVKPVTVEDSEVEAELDALRARFGTLKGVERAAE
NGDFVSIDLSATVDGKEVEEAATTGLSHEIGSGQLIDGLDEAIIGLKAGEEKVFTTKLAAGEFAGQEAEVTVKVGSIKER
ELPDADDDFAQLASEFDTIGELKDSLTEQVKGRKRIAQADEIRDETITALLDKIEIPVPEKILEEQIGNNLHEAVHGLDH
NEERLNELLQEQGSSREEFDKDMRESATKSIKTELLLDVIADKFDINVDQQDLTERLVLMSRQYGIEPQQLVQYLTQQQQ
LPGLYVDVRRGKAIAEVIRQAKVTDSTGADVDVDAVLGPRRGGADEAGAEAEAAEEKPAKAKKSADSEKTDKSEKAEKKS
KKKSKDDDAE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=435, Percent_Identity=25.5172413793103, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_MYCA9 (B1MMV3)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001702319.1
- ProteinModelPortal:   B1MMV3
- SMR:   B1MMV3
- EnsemblBacteria:   EBMYCT00000008467
- GeneID:   5967508
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_1580
- GeneTree:   EBGT00050000015814
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- BioCyc:   MABS561007:MAB_1580-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 53473; Mature: 53473

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTVEKLSPTRVRINVEVPFAELEPDFSKAYKELAQQVRLPGFRPGKAPAKLLEARVGR
CCCCHHHCCCCEEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AAVLEQVVNAALPARYSEAVTASDVKPLGQPEIEVTKIEDGQELTFTAEVDVRPEIELPD
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCC
LSGVAVTVKPVTVEDSEVEAELDALRARFGTLKGVERAAENGDFVSIDLSATVDGKEVEE
CCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHH
AATTGLSHEIGSGQLIDGLDEAIIGLKAGEEKVFTTKLAAGEFAGQEAEVTVKVGSIKER
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHEEEHHHHHHHCCCCCEEEEEECCCCCC
ELPDADDDFAQLASEFDTIGELKDSLTEQVKGRKRIAQADEIRDETITALLDKIEIPVPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KILEEQIGNNLHEAVHGLDHNEERLNELLQEQGSSREEFDKDMRESATKSIKTELLLDVI
HHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ADKFDINVDQQDLTERLVLMSRQYGIEPQQLVQYLTQQQQLPGLYVDVRRGKAIAEVIRQ
HHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
AKVTDSTGADVDVDAVLGPRRGGADEAGAEAEAAEEKPAKAKKSADSEKTDKSEKAEKKS
HHCCCCCCCCCEEHHEECCCCCCCCCCCCCHHHHHHCCHHHHHCCCCHHCCHHHHHHHHH
KKKSKDDDAE
HHCCCCCCCC
>Mature Secondary Structure
MKSTVEKLSPTRVRINVEVPFAELEPDFSKAYKELAQQVRLPGFRPGKAPAKLLEARVGR
CCCCHHHCCCCEEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AAVLEQVVNAALPARYSEAVTASDVKPLGQPEIEVTKIEDGQELTFTAEVDVRPEIELPD
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCC
LSGVAVTVKPVTVEDSEVEAELDALRARFGTLKGVERAAENGDFVSIDLSATVDGKEVEE
CCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHH
AATTGLSHEIGSGQLIDGLDEAIIGLKAGEEKVFTTKLAAGEFAGQEAEVTVKVGSIKER
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHEEEHHHHHHHCCCCCEEEEEECCCCCC
ELPDADDDFAQLASEFDTIGELKDSLTEQVKGRKRIAQADEIRDETITALLDKIEIPVPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KILEEQIGNNLHEAVHGLDHNEERLNELLQEQGSSREEFDKDMRESATKSIKTELLLDVI
HHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ADKFDINVDQQDLTERLVLMSRQYGIEPQQLVQYLTQQQQLPGLYVDVRRGKAIAEVIRQ
HHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
AKVTDSTGADVDVDAVLGPRRGGADEAGAEAEAAEEKPAKAKKSADSEKTDKSEKAEKKS
HHCCCCCCCCCEEHHEECCCCCCCCCCCCCHHHHHHCCHHHHHCCCCHHCCHHHHHHHHH
KKKSKDDDAE
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA