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The map label for this gene is cysNC [H]

Identifier: 169628634

GI number: 169628634

Start: 1565197

End: 1567074

Strand: Reverse

Name: cysNC [H]

Synonym: MAB_1544c

Alternate gene names: 169628634

Gene position: 1567074-1565197 (Counterclockwise)

Preceding gene: 169628635

Following gene: 169628632

Centisome position: 30.93

GC content: 65.5

Gene sequence:

>1878_bases
ATGAGCACCCCAACCGCGGTGGACACCCGCCAACTGCTGCGTATCGCCACCGCCGGCTCGGTGGACGACGGCAAGAGCAC
GCTCATCGGACGGCTGCTGCACGACACCGACAGTCTGCCGGTGGACCATCTGGAAGCCGTGACCGACGATGAGGGCGTGG
CCGATCTGGCTGCGCTTTCCGATGGCCTGCGTGCCGAACGCGAACAGGGCATCACCATCGACGTGGCCTACCGCTTCTTC
TCCACAGAGAGTCGCAGCTACATCCTGGCCGACACTCCCGGTCACGAGCGCTACACCCGCAACATGTTCACCGGTGCCTC
CAACGCGCATGTGGCGATCCTGCTGGTCGACGCGCGGGCCGGCGTGCTGCGGCAGACCCGCCGCCATGCCCGCATCGCAA
AGCTGTTGGGCATCAAGCACTTCGTGGCTGCGGTCAACAAGATCGACCTGGTCGACTTCGACGCACAACGGTTCACGGAA
GTCGATCGCGAGCTGCATCTGCTAGCCGACCGGCTCGGCAAGGTAGAGATCACGGTCATTCCGATCGCCGCCAAACATGG
CGACAACGTGGTGCACCGCTCCGAGAACACTCCGTGGTACTCGGGGCCGACACTCCTCGAATACCTTGAAAACGTAGAGC
TTTCACCCCCACAGCCAGAGGCAGCCAAGCTACGACTGCCCGTGCAGTGGGTCTCACGCCCCACCGCCGAACAACGTCGC
CGATACACCGGCCGCCTGGCAGCGGGCACGCTCAGTGTCGGTGACTCGGTGGTCAATCTGCCCTCGGGCACTCGCTCGAC
GGTGACGGTGGTCGACACCCTGGACGAAAGTCGTGCCACTGGTGTGGCCCCCCTGTCGGTATCGATCGAATTGGCCGACG
ACATCGACGTGGGGCGCGGCGACGTGCTGGTGAGCGGTGCCGATGACGCGGTGCTGCCGGTCCTCGCCCGCGAGATCGAC
GCCACGGTCTGCTGGTTTACCAACGGACCACTACGCGCGGGCGATCGACTGGCACTCAAGCAGGGCACCCGGACGGTGCG
GGCCACGGTTCAGGCTCTGCACACCCGCCTTGACCCGGAGACACTCGACGAGTTGGACGGGCCGGTCGAGTTGGCGCTCA
ACGACATCGGCGCGGTCACCCTGCGCACCAGTTCGGTGGTGGTAGCCGATTCCTATGCCGACAGCCGGGACAGCGGCGCG
TTCATCCTGATCGACGAGGCGTCCAATGACACCGTCGGCGCCGGAACCATCACCGAGGCGCGCGAGGTCAAGCCCGAGAC
GCACTCACGCACCGATATCCGGTGGCACCCATCGGCGCTGGATCGTGAATACCGTTGGGACAGCACCGCTCAGCGCGGCG
CCATCATCTGGTTCACCGGCCTGCCGGCATCCGGCAAATCGACGATCGCGGTGGCGGTGGAACGTGCGCTGGTGGAGTCG
GGGCAGGTCGCATACCTGCTCGACGGCGATAATCTGCGCCACGGGTTATCCGATGACCTCGGGTTCTCTCCGGGCGACCG
GGCCGAGAACATCCGTCGGGTGTCGCATCTGACGCGGCTGTTTGCCGATGCCGGCGTGGTGGCATTGGCATCGCTGGTGT
CGCCCCTGCGCTCCGATCGCGAGACCGCACGCAGCATCAACGCCGCCGCGAAACTGCCGTTCATCGAGGTGTACATCGCT
ACGCCACTGGCCGAATGCGAGAAGCGAGACCCCAAGGGGCTGTACGCGCGGGCCCGCGCCGGCGAGCTGAAGGGTTTGAC
GGGCGTGGAAGCACCTTATGAAGCCCCGGAGAATCCTGAACTCGTCCTCGATACCACCGGGGCCGATATCGACGAGCTGG
TTGCTCAGGTACTCGACGTTCTTAACCGCGCTCGCTGA

Upstream 100 bases:

>100_bases
AAATCTCCGTGGCCACCGTCTCCGAGCGCGGCGAGACCCGCGCCGACGACCGCACATCCGTGGCGGCCATGGAAGACCGG
AAACGAGAGGGCTACTTCTG

Downstream 100 bases:

>100_bases
GCGCGGTTAAGAACGAACCGGGTTACGTGCGGTTGGCCGCGCGCTCCAGCACGGGGCGCTGCTCCGGGCAGTACTCATCG
ATCGAAGCGGACAGGAACTG

Product: CysN/CysC bifunctional enzyme

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSTPTAVDTRQLLRIATAGSVDDGKSTLIGRLLHDTDSLPVDHLEAVTDDEGVADLAALSDGLRAEREQGITIDVAYRFF
STESRSYILADTPGHERYTRNMFTGASNAHVAILLVDARAGVLRQTRRHARIAKLLGIKHFVAAVNKIDLVDFDAQRFTE
VDRELHLLADRLGKVEITVIPIAAKHGDNVVHRSENTPWYSGPTLLEYLENVELSPPQPEAAKLRLPVQWVSRPTAEQRR
RYTGRLAAGTLSVGDSVVNLPSGTRSTVTVVDTLDESRATGVAPLSVSIELADDIDVGRGDVLVSGADDAVLPVLAREID
ATVCWFTNGPLRAGDRLALKQGTRTVRATVQALHTRLDPETLDELDGPVELALNDIGAVTLRTSSVVVADSYADSRDSGA
FILIDEASNDTVGAGTITEAREVKPETHSRTDIRWHPSALDREYRWDSTAQRGAIIWFTGLPASGKSTIAVAVERALVES
GQVAYLLDGDNLRHGLSDDLGFSPGDRAENIRRVSHLTRLFADAGVVALASLVSPLRSDRETARSINAAAKLPFIEVYIA
TPLAECEKRDPKGLYARARAGELKGLTGVEAPYEAPENPELVLDTTGADIDELVAQVLDVLNRAR

Sequences:

>Translated_625_residues
MSTPTAVDTRQLLRIATAGSVDDGKSTLIGRLLHDTDSLPVDHLEAVTDDEGVADLAALSDGLRAEREQGITIDVAYRFF
STESRSYILADTPGHERYTRNMFTGASNAHVAILLVDARAGVLRQTRRHARIAKLLGIKHFVAAVNKIDLVDFDAQRFTE
VDRELHLLADRLGKVEITVIPIAAKHGDNVVHRSENTPWYSGPTLLEYLENVELSPPQPEAAKLRLPVQWVSRPTAEQRR
RYTGRLAAGTLSVGDSVVNLPSGTRSTVTVVDTLDESRATGVAPLSVSIELADDIDVGRGDVLVSGADDAVLPVLAREID
ATVCWFTNGPLRAGDRLALKQGTRTVRATVQALHTRLDPETLDELDGPVELALNDIGAVTLRTSSVVVADSYADSRDSGA
FILIDEASNDTVGAGTITEAREVKPETHSRTDIRWHPSALDREYRWDSTAQRGAIIWFTGLPASGKSTIAVAVERALVES
GQVAYLLDGDNLRHGLSDDLGFSPGDRAENIRRVSHLTRLFADAGVVALASLVSPLRSDRETARSINAAAKLPFIEVYIA
TPLAECEKRDPKGLYARARAGELKGLTGVEAPYEAPENPELVLDTTGADIDELVAQVLDVLNRAR
>Mature_624_residues
STPTAVDTRQLLRIATAGSVDDGKSTLIGRLLHDTDSLPVDHLEAVTDDEGVADLAALSDGLRAEREQGITIDVAYRFFS
TESRSYILADTPGHERYTRNMFTGASNAHVAILLVDARAGVLRQTRRHARIAKLLGIKHFVAAVNKIDLVDFDAQRFTEV
DRELHLLADRLGKVEITVIPIAAKHGDNVVHRSENTPWYSGPTLLEYLENVELSPPQPEAAKLRLPVQWVSRPTAEQRRR
YTGRLAAGTLSVGDSVVNLPSGTRSTVTVVDTLDESRATGVAPLSVSIELADDIDVGRGDVLVSGADDAVLPVLAREIDA
TVCWFTNGPLRAGDRLALKQGTRTVRATVQALHTRLDPETLDELDGPVELALNDIGAVTLRTSSVVVADSYADSRDSGAF
ILIDEASNDTVGAGTITEAREVKPETHSRTDIRWHPSALDREYRWDSTAQRGAIIWFTGLPASGKSTIAVAVERALVESG
QVAYLLDGDNLRHGLSDDLGFSPGDRAENIRRVSHLTRLFADAGVVALASLVSPLRSDRETARSINAAAKLPFIEVYIAT
PLAECEKRDPKGLYARARAGELKGLTGVEAPYEAPENPELVLDTTGADIDELVAQVLDVLNRAR

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI46094058, Length=171, Percent_Identity=52.046783625731, Blast_Score=202, Evalue=9e-52,
Organism=Homo sapiens, GI34447231, Length=171, Percent_Identity=53.2163742690059, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI62912492, Length=171, Percent_Identity=53.2163742690059, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI223555963, Length=438, Percent_Identity=29.9086757990868, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI5729864, Length=438, Percent_Identity=29.9086757990868, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI4503471, Length=437, Percent_Identity=29.9771167048055, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=28.0353200883002, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI46094014, Length=440, Percent_Identity=25.2272727272727, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI194018522, Length=438, Percent_Identity=25.5707762557078, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI194018520, Length=438, Percent_Identity=25.5707762557078, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI194097354, Length=438, Percent_Identity=25.5707762557078, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI34147630, Length=172, Percent_Identity=33.1395348837209, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1789108, Length=419, Percent_Identity=47.9713603818616, Blast_Score=348, Evalue=6e-97,
Organism=Escherichia coli, GI1789107, Length=192, Percent_Identity=48.4375, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1789737, Length=291, Percent_Identity=28.8659793814433, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790412, Length=291, Percent_Identity=28.8659793814433, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI2367247, Length=174, Percent_Identity=33.9080459770115, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17542422, Length=195, Percent_Identity=47.1794871794872, Blast_Score=192, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17552884, Length=437, Percent_Identity=29.9771167048055, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=437, Percent_Identity=29.9771167048055, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=425, Percent_Identity=28.7058823529412, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI115532065, Length=425, Percent_Identity=28.7058823529412, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=272, Percent_Identity=29.7794117647059, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=27.906976744186, Blast_Score=122, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=167, Percent_Identity=34.7305389221557, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=156, Percent_Identity=32.6923076923077, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=156, Percent_Identity=32.6923076923077, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322852, Length=191, Percent_Identity=49.2146596858639, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6325337, Length=435, Percent_Identity=29.8850574712644, Blast_Score=153, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=435, Percent_Identity=29.8850574712644, Blast_Score=153, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=280, Percent_Identity=30.7142857142857, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320377, Length=442, Percent_Identity=26.6968325791855, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324761, Length=248, Percent_Identity=30.6451612903226, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24667032, Length=172, Percent_Identity=50.5813953488372, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24667028, Length=172, Percent_Identity=50.5813953488372, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24667036, Length=172, Percent_Identity=50.5813953488372, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24667040, Length=172, Percent_Identity=50.5813953488372, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI116007838, Length=172, Percent_Identity=50.5813953488372, Blast_Score=182, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24667044, Length=172, Percent_Identity=50.5813953488372, Blast_Score=182, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24652838, Length=293, Percent_Identity=32.0819112627986, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137572, Length=293, Percent_Identity=32.0819112627986, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45553807, Length=297, Percent_Identity=31.986531986532, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45553816, Length=297, Percent_Identity=31.986531986532, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24651721, Length=297, Percent_Identity=31.986531986532, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17864154, Length=297, Percent_Identity=31.986531986532, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45550900, Length=437, Percent_Identity=28.8329519450801, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137380, Length=440, Percent_Identity=25.9090909090909, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI281363316, Length=279, Percent_Identity=30.8243727598566, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17864358, Length=279, Percent_Identity=30.8243727598566, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19921738, Length=208, Percent_Identity=30.2884615384615, Blast_Score=91, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 67642; Mature: 67510

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPTAVDTRQLLRIATAGSVDDGKSTLIGRLLHDTDSLPVDHLEAVTDDEGVADLAALS
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHH
DGLRAEREQGITIDVAYRFFSTESRSYILADTPGHERYTRNMFTGASNAHVAILLVDARA
HHHHHHHHCCCEEEEEEEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEEECCC
GVLRQTRRHARIAKLLGIKHFVAAVNKIDLVDFDAQRFTEVDRELHLLADRLGKVEITVI
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEE
PIAAKHGDNVVHRSENTPWYSGPTLLEYLENVELSPPQPEAAKLRLPVQWVSRPTAEQRR
EEEECCCCCEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCHHHHCCCCHHHHH
RYTGRLAAGTLSVGDSVVNLPSGTRSTVTVVDTLDESRATGVAPLSVSIELADDIDVGRG
HHCCCEEECEEECCCCEEECCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCC
DVLVSGADDAVLPVLAREIDATVCWFTNGPLRAGDRLALKQGTRTVRATVQALHTRLDPE
CEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHCCHHHHHHHHHHHHCCCHH
TLDELDGPVELALNDIGAVTLRTSSVVVADSYADSRDSGAFILIDEASNDTVGAGTITEA
HHHHCCCCEEEEECCCCEEEEEECEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHH
REVKPETHSRTDIRWHPSALDREYRWDSTAQRGAIIWFTGLPASGKSTIAVAVERALVES
HHCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
GQVAYLLDGDNLRHGLSDDLGFSPGDRAENIRRVSHLTRLFADAGVVALASLVSPLRSDR
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ETARSINAAAKLPFIEVYIATPLAECEKRDPKGLYARARAGELKGLTGVEAPYEAPENPE
HHHHHHHHHHCCCEEEEEEECCHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
LVLDTTGADIDELVAQVLDVLNRAR
EEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STPTAVDTRQLLRIATAGSVDDGKSTLIGRLLHDTDSLPVDHLEAVTDDEGVADLAALS
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHH
DGLRAEREQGITIDVAYRFFSTESRSYILADTPGHERYTRNMFTGASNAHVAILLVDARA
HHHHHHHHCCCEEEEEEEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEEECCC
GVLRQTRRHARIAKLLGIKHFVAAVNKIDLVDFDAQRFTEVDRELHLLADRLGKVEITVI
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEE
PIAAKHGDNVVHRSENTPWYSGPTLLEYLENVELSPPQPEAAKLRLPVQWVSRPTAEQRR
EEEECCCCCEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCHHHHCCCCHHHHH
RYTGRLAAGTLSVGDSVVNLPSGTRSTVTVVDTLDESRATGVAPLSVSIELADDIDVGRG
HHCCCEEECEEECCCCEEECCCCCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCC
DVLVSGADDAVLPVLAREIDATVCWFTNGPLRAGDRLALKQGTRTVRATVQALHTRLDPE
CEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHCCHHHHHHHHHHHHCCCHH
TLDELDGPVELALNDIGAVTLRTSSVVVADSYADSRDSGAFILIDEASNDTVGAGTITEA
HHHHCCCCEEEEECCCCEEEEEECEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHH
REVKPETHSRTDIRWHPSALDREYRWDSTAQRGAIIWFTGLPASGKSTIAVAVERALVES
HHCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
GQVAYLLDGDNLRHGLSDDLGFSPGDRAENIRRVSHLTRLFADAGVVALASLVSPLRSDR
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ETARSINAAAKLPFIEVYIATPLAECEKRDPKGLYARARAGELKGLTGVEAPYEAPENPE
HHHHHHHHHHCCCEEEEEEECCHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
LVLDTTGADIDELVAQVLDVLNRAR
EEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]