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The map label for this gene is phnF [H]

Identifier: 169628589

GI number: 169628589

Start: 1511797

End: 1512504

Strand: Reverse

Name: phnF [H]

Synonym: MAB_1498c

Alternate gene names: 169628589

Gene position: 1512504-1511797 (Counterclockwise)

Preceding gene: 169628597

Following gene: 169628588

Centisome position: 29.85

GC content: 64.12

Gene sequence:

>708_bases
ATGACAGCCGATGTGCGACTGCCCAAGCATTACCAGGTACGCACCGAACTGGAACACATTCTCTCGGCACTCAACGAGGG
AGACCCCGTGCCGCCCGAGCGCGACCTGGCCGAACGATTCGCGGTGTCGCGAGAAACCGTGCGCCAGGCACTGCATGAGC
TACTGGTGGAGGGACGTATCGAACGGCGTGGGCGCGGAACGGTGGTGGCGGCACCAAAACTATTGCAGCCGCTGTCTTTA
CGCTCCTATACCGAGGGGGCGCAAAGCCAGGGGCGAACACCGGGGCGGTTACTGGTCACCTGGGAGAACATCACCGGCGA
CGCCGACCTGTGCCGGGACCTCGGAATCCGCGGCGGGTCGACGGTGATCCACCTGGAGCGCGTCTTGCTCGCCGATGGCG
CCAAGCTTGGGCTGGAGAGCACATACCTGGCCAAAGCACGCTTTGCCGAGTTGCGCAACACCTTTGACCCCAGTACCTCG
CTGTACGCGGCGATTCGCGCGCTAGGAGTTCAGTTCGGCAGTGCCGTCGAACGCATCGAAACAGTACTCGCCTCACCCCG
CGAGGCATCGCTGCTGGACACCTCGATCGCCATGCCCATGCTCCTCATGAACCGGCGCACCCTCGACACCGATGGGCGCC
CGATTGAACGGGTGCGCTCCCTGTTCCGCGGAGACCGTGTGGCCTTCGAGGCGGTACTGAAGGAGTGA

Upstream 100 bases:

>100_bases
AACTCGAGGCCGTTCCGAAGTCACTGCGCACCGTGCGCGACAAGGAAGCCGCCGGTTCCATTCCGGGCAGCCTCGGGACG
GCCCGAAAGGTGGTGGCGCC

Downstream 100 bases:

>100_bases
CATAAGTGTACGGTATGCGTACACTTATGTCATGGTTGCCCGCGGAGAGACACGTAGCGCCGCGCTCCTCGCGGTCGCAT
TGCAGGTACTGATACGCGAT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTADVRLPKHYQVRTELEHILSALNEGDPVPPERDLAERFAVSRETVRQALHELLVEGRIERRGRGTVVAAPKLLQPLSL
RSYTEGAQSQGRTPGRLLVTWENITGDADLCRDLGIRGGSTVIHLERVLLADGAKLGLESTYLAKARFAELRNTFDPSTS
LYAAIRALGVQFGSAVERIETVLASPREASLLDTSIAMPMLLMNRRTLDTDGRPIERVRSLFRGDRVAFEAVLKE

Sequences:

>Translated_235_residues
MTADVRLPKHYQVRTELEHILSALNEGDPVPPERDLAERFAVSRETVRQALHELLVEGRIERRGRGTVVAAPKLLQPLSL
RSYTEGAQSQGRTPGRLLVTWENITGDADLCRDLGIRGGSTVIHLERVLLADGAKLGLESTYLAKARFAELRNTFDPSTS
LYAAIRALGVQFGSAVERIETVLASPREASLLDTSIAMPMLLMNRRTLDTDGRPIERVRSLFRGDRVAFEAVLKE
>Mature_234_residues
TADVRLPKHYQVRTELEHILSALNEGDPVPPERDLAERFAVSRETVRQALHELLVEGRIERRGRGTVVAAPKLLQPLSLR
SYTEGAQSQGRTPGRLLVTWENITGDADLCRDLGIRGGSTVIHLERVLLADGAKLGLESTYLAKARFAELRNTFDPSTSL
YAAIRALGVQFGSAVERIETVLASPREASLLDTSIAMPMLLMNRRTLDTDGRPIERVRSLFRGDRVAFEAVLKE

Specific function: Represses the phnDCE operon, involved in the uptake of phosphate, under conditions of phosphate availability in the cell [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082252, Length=204, Percent_Identity=25, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790540, Length=211, Percent_Identity=31.2796208530806, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788418, Length=208, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 26067; Mature: 25936

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADVRLPKHYQVRTELEHILSALNEGDPVPPERDLAERFAVSRETVRQALHELLVEGRI
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRGRGTVVAAPKLLQPLSLRSYTEGAQSQGRTPGRLLVTWENITGDADLCRDLGIRGGS
HHCCCCCEEECHHHHCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCC
TVIHLERVLLADGAKLGLESTYLAKARFAELRNTFDPSTSLYAAIRALGVQFGSAVERIE
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TVLASPREASLLDTSIAMPMLLMNRRTLDTDGRPIERVRSLFRGDRVAFEAVLKE
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
TADVRLPKHYQVRTELEHILSALNEGDPVPPERDLAERFAVSRETVRQALHELLVEGRI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRGRGTVVAAPKLLQPLSLRSYTEGAQSQGRTPGRLLVTWENITGDADLCRDLGIRGGS
HHCCCCCEEECHHHHCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCC
TVIHLERVLLADGAKLGLESTYLAKARFAELRNTFDPSTSLYAAIRALGVQFGSAVERIE
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TVLASPREASLLDTSIAMPMLLMNRRTLDTDGRPIERVRSLFRGDRVAFEAVLKE
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA