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The map label for this gene is yyaE [H]

Identifier: 169628586

GI number: 169628586

Start: 1507541

End: 1509772

Strand: Direct

Name: yyaE [H]

Synonym: MAB_1495

Alternate gene names: 169628586

Gene position: 1507541-1509772 (Clockwise)

Preceding gene: 169628585

Following gene: 169628590

Centisome position: 29.75

GC content: 62.19

Gene sequence:

>2232_bases
ATGACCGCCGAATGGTTGCCCACCGCATGCATCTTGTGTGAATGTAACTGCGGCATCGTGGTGCAGACGGAGGGGCGCAC
CCTCGCGAAGATCCGCGGTGACAAGGATCATCCGGGTACCAAAGGGTATACCTGCAATAAGGCGTTGCGGCTGAATCACT
ATCAGAACAATGCGAACCGGCTGACATCGCCCATGCGTCGCCGTCCCGATGGCACCTATGAGGAAATCGATTGGGATACG
GCGATCGTGGAGATCGCCGAGGGATTCAAACACATCCGCGACAGCTACGGCGGCGACAAGATCTTCTACTACGGCGGTGG
TGGGCAAGGTAACCATCTGGGTGGTGCCTACAGCGGCGCATTCCTCAAAGCCATTGGGTCCAAGTACCGTTCCAATGCGC
TAGCCCAGGAGAAGACGGGTGAGGCCTGGATCGACAAGAATCTGTACGGCGGACACACTCGCGGCGAATTCGAGTATGCG
GAGGTGTCCGTCTTCGTCGGGAAGAACCCGTGGATGTCGCAGAGCTTTCCGCGGGCTCGTGTGGTGCTCAATGAGATTGC
CAAGGATCCGCAGCGCTCGATGATCGTCATCGATCCGGTTAACACCGATACCGCCAAGATGGCGGACTTTCATCTACGCG
TACGCCCCGGTACAGATGCCTGGTGCCTCGCCGCGCTGGCGGCGGTGCTGGTGCAGGAGAATCTCTGTGACGAAGCATTC
CTCGCCGAACACGTCACCGGAACCGAAGAGGTTCGGGATGTGCTGCGCACGGTGCCGATCGCGGACTACGCACGGCGCTG
CGGGGTGGACGAGGACCTCATTCGGCGGGCGGCCCGCCGGGTTGCGGCGGCCGAGAGCGTGGCCGTCTTCGAAGACCTGG
GCATCCAGCAGGCCCCCAACAGTACGCTGAGCTCATATCTGAACAAGATGCTCTGGATCCTCACCGGGAACTTCGCAAAA
CGCGGTGGGCAGCATCTGCATTCGTGGATGGCGCCGCTCATGGGTGGCTTCTTCGGTTACACCCCGGTGACCGGGGCCCG
CATCATCGGTGGCTTGGTGCCCTCCAATGTCATCCCGGAAGAGATCCTCACCGATCACCCGGATCGTTTCCGCGCGATGA
TCGTCGAGAGTAGCAATCCCGCACATTCCCTTGCGGATTCGGCGCGGTGCAAGGAGGCCTTCGCTGCCCTGGAACTCTTG
GTGGTCGTGGATGTGGCGATGACCGAGACCGCGCGGCTCGCACACTACGTGCTGCCCGCCGCCACACAGTTCGAGAAGGT
AGAGGCCACCTTCTTCAATCTGGAATTCCCGCACAACACCTTTCAACTGCGACACCCACTATTCGAACCCGAGTCAGGAA
CCCTGCCCGAACCCGAGATCTGGGCGCGGCTGGTGCGGGCGCTGGGAGTGGTTGACGACGCGGATCTGCAGCCATTACGT
GAGGCCGCCGCGCAGGGGCGGGAAGCATACTTGCAGGCATTTTTCGCAGTACTGGCCAATCCGGTACTCGGTGCATTGGC
TCCGTACGTCCTCTACGAGACCTTGGGCCCCACACTGCCGAGGGGATTGGAAGGCGCTGCAGCTCTCTGGGGCCTGGCGC
AGAAGGCGATGATGACCTACCCGCAGGCGGTGGCTCGGGCCGGTCACCCCGACGGCAACGCCTTGTTCGACGCGATCCTG
AACAGCCCATCCGGCGTGACCTTTACCGTCCACGACTATGAGGACGACTTCGCCCTCATCACGCACGCGGACCGCAAGAT
CGCTCTCGCTATTCCCGAAATGCTTGATGAGATAAAGGGGCTGGTGTCGCGTCCGGCACAGCTGACAACGCCGGAATTTC
CGATCGTGCTGTCGGCCGGCGAGCGCAGGGCATACACCGCCAACGACATCCTGCGTGACCCGTCCTGGCGCAAGCGTGAT
CAGCAGGGAGCTCTGCGGATCAGTCCGCAGGATGCACAACAGCTCGGTGTCGCTGACGGTGAAAGGGTTCGGATCGCGAC
AGCGGCCGGTGCCGCCGAGGCCACGGTCGAGATCAGCGACATGATGCAGCCCGGTCACGCCTCGCTGCCCAATGGTTTCG
GCCTCGACTACACCGACGAGTCCGGGGAAACACATATACCCGGGGTGGCGCCGAATGCTCTCACCTCGTCGGCTTGGCGT
GACGAGTACGCCGGAACGCCATGGCACAAGCATGTCCCCGCGCGTTTGGAGACGCTGACCGCCGCCATCTAG

Upstream 100 bases:

>100_bases
CTCTGGAAGGCGCAATCATTATCGCGCGATCGACTCGCGACATCACGCCAATCGACGTGGTGCACAGGCAGTTACGCACT
CTCATTGAGGAGGCCGGACA

Downstream 100 bases:

>100_bases
ACAACACGGGCGAGATACTCGAGGACTGTGCGCCCGGATTCGGTCATGACACCGATGTACCCGTCCGGGCGTACCAGCAC
CAGTGTCGTTGCCGTAACGT

Product: oxidoreductase

Products: nitrate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MTAEWLPTACILCECNCGIVVQTEGRTLAKIRGDKDHPGTKGYTCNKALRLNHYQNNANRLTSPMRRRPDGTYEEIDWDT
AIVEIAEGFKHIRDSYGGDKIFYYGGGGQGNHLGGAYSGAFLKAIGSKYRSNALAQEKTGEAWIDKNLYGGHTRGEFEYA
EVSVFVGKNPWMSQSFPRARVVLNEIAKDPQRSMIVIDPVNTDTAKMADFHLRVRPGTDAWCLAALAAVLVQENLCDEAF
LAEHVTGTEEVRDVLRTVPIADYARRCGVDEDLIRRAARRVAAAESVAVFEDLGIQQAPNSTLSSYLNKMLWILTGNFAK
RGGQHLHSWMAPLMGGFFGYTPVTGARIIGGLVPSNVIPEEILTDHPDRFRAMIVESSNPAHSLADSARCKEAFAALELL
VVVDVAMTETARLAHYVLPAATQFEKVEATFFNLEFPHNTFQLRHPLFEPESGTLPEPEIWARLVRALGVVDDADLQPLR
EAAAQGREAYLQAFFAVLANPVLGALAPYVLYETLGPTLPRGLEGAAALWGLAQKAMMTYPQAVARAGHPDGNALFDAIL
NSPSGVTFTVHDYEDDFALITHADRKIALAIPEMLDEIKGLVSRPAQLTTPEFPIVLSAGERRAYTANDILRDPSWRKRD
QQGALRISPQDAQQLGVADGERVRIATAAGAAEATVEISDMMQPGHASLPNGFGLDYTDESGETHIPGVAPNALTSSAWR
DEYAGTPWHKHVPARLETLTAAI

Sequences:

>Translated_743_residues
MTAEWLPTACILCECNCGIVVQTEGRTLAKIRGDKDHPGTKGYTCNKALRLNHYQNNANRLTSPMRRRPDGTYEEIDWDT
AIVEIAEGFKHIRDSYGGDKIFYYGGGGQGNHLGGAYSGAFLKAIGSKYRSNALAQEKTGEAWIDKNLYGGHTRGEFEYA
EVSVFVGKNPWMSQSFPRARVVLNEIAKDPQRSMIVIDPVNTDTAKMADFHLRVRPGTDAWCLAALAAVLVQENLCDEAF
LAEHVTGTEEVRDVLRTVPIADYARRCGVDEDLIRRAARRVAAAESVAVFEDLGIQQAPNSTLSSYLNKMLWILTGNFAK
RGGQHLHSWMAPLMGGFFGYTPVTGARIIGGLVPSNVIPEEILTDHPDRFRAMIVESSNPAHSLADSARCKEAFAALELL
VVVDVAMTETARLAHYVLPAATQFEKVEATFFNLEFPHNTFQLRHPLFEPESGTLPEPEIWARLVRALGVVDDADLQPLR
EAAAQGREAYLQAFFAVLANPVLGALAPYVLYETLGPTLPRGLEGAAALWGLAQKAMMTYPQAVARAGHPDGNALFDAIL
NSPSGVTFTVHDYEDDFALITHADRKIALAIPEMLDEIKGLVSRPAQLTTPEFPIVLSAGERRAYTANDILRDPSWRKRD
QQGALRISPQDAQQLGVADGERVRIATAAGAAEATVEISDMMQPGHASLPNGFGLDYTDESGETHIPGVAPNALTSSAWR
DEYAGTPWHKHVPARLETLTAAI
>Mature_742_residues
TAEWLPTACILCECNCGIVVQTEGRTLAKIRGDKDHPGTKGYTCNKALRLNHYQNNANRLTSPMRRRPDGTYEEIDWDTA
IVEIAEGFKHIRDSYGGDKIFYYGGGGQGNHLGGAYSGAFLKAIGSKYRSNALAQEKTGEAWIDKNLYGGHTRGEFEYAE
VSVFVGKNPWMSQSFPRARVVLNEIAKDPQRSMIVIDPVNTDTAKMADFHLRVRPGTDAWCLAALAAVLVQENLCDEAFL
AEHVTGTEEVRDVLRTVPIADYARRCGVDEDLIRRAARRVAAAESVAVFEDLGIQQAPNSTLSSYLNKMLWILTGNFAKR
GGQHLHSWMAPLMGGFFGYTPVTGARIIGGLVPSNVIPEEILTDHPDRFRAMIVESSNPAHSLADSARCKEAFAALELLV
VVDVAMTETARLAHYVLPAATQFEKVEATFFNLEFPHNTFQLRHPLFEPESGTLPEPEIWARLVRALGVVDDADLQPLRE
AAAQGREAYLQAFFAVLANPVLGALAPYVLYETLGPTLPRGLEGAAALWGLAQKAMMTYPQAVARAGHPDGNALFDAILN
SPSGVTFTVHDYEDDFALITHADRKIALAIPEMLDEIKGLVSRPAQLTTPEFPIVLSAGERRAYTANDILRDPSWRKRDQ
QGALRISPQDAQQLGVADGERVRIATAAGAAEATVEISDMMQPGHASLPNGFGLDYTDESGETHIPGVAPNALTSSAWRD
EYAGTPWHKHVPARLETLTAAI

Specific function: Decomposes Formic Acid To Hydrogen And Carbon Dioxide Under Anaerobic Conditions In The Absence Of Exogenous Electron Acceptors. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=458, Percent_Identity=26.6375545851528, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787870, Length=804, Percent_Identity=23.8805970149254, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI87081797, Length=819, Percent_Identity=24.1758241758242, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI171474008, Length=361, Percent_Identity=25.7617728531856, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI3868719, Length=282, Percent_Identity=25.177304964539, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788534, Length=287, Percent_Identity=25.0871080139373, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787231, Length=286, Percent_Identity=26.5734265734266, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.7.99.4

Molecular weight: Translated: 81242; Mature: 81111

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEWLPTACILCECNCGIVVQTEGRTLAKIRGDKDHPGTKGYTCNKALRLNHYQNNANR
CCCCCCCCEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCEECCCEEEECCCCCCHHH
LTSPMRRRPDGTYEEIDWDTAIVEIAEGFKHIRDSYGGDKIFYYGGGGQGNHLGGAYSGA
HHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHH
FLKAIGSKYRSNALAQEKTGEAWIDKNLYGGHTRGEFEYAEVSVFVGKNPWMSQSFPRAR
HHHHHHHHHHHCCHHHHCCCCHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHH
VVLNEIAKDPQRSMIVIDPVNTDTAKMADFHLRVRPGTDAWCLAALAAVLVQENLCDEAF
HHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHH
LAEHVTGTEEVRDVLRTVPIADYARRCGVDEDLIRRAARRVAAAESVAVFEDLGIQQAPN
HHHHCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
STLSSYLNKMLWILTGNFAKRGGQHLHSWMAPLMGGFFGYTPVTGARIIGGLVPSNVIPE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCH
EILTDHPDRFRAMIVESSNPAHSLADSARCKEAFAALELLVVVDVAMTETARLAHYVLPA
HHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ATQFEKVEATFFNLEFPHNTFQLRHPLFEPESGTLPEPEIWARLVRALGVVDDADLQPLR
HHHHHHHEEEEEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
EAAAQGREAYLQAFFAVLANPVLGALAPYVLYETLGPTLPRGLEGAAALWGLAQKAMMTY
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
PQAVARAGHPDGNALFDAILNSPSGVTFTVHDYEDDFALITHADRKIALAIPEMLDEIKG
HHHHHHCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCEEEEHHHHHHHHHHH
LVSRPAQLTTPEFPIVLSAGERRAYTANDILRDPSWRKRDQQGALRISPQDAQQLGVADG
HHCCCCCCCCCCCCEEEECCCCCCEEHHHHHCCCCCCCCCCCCCEEECCHHHHHCCCCCC
ERVRIATAAGAAEATVEISDMMQPGHASLPNGFGLDYTDESGETHIPGVAPNALTSSAWR
CEEEEEEECCCCHHEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DEYAGTPWHKHVPARLETLTAAI
HHCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TAEWLPTACILCECNCGIVVQTEGRTLAKIRGDKDHPGTKGYTCNKALRLNHYQNNANR
CCCCCCCEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCEECCCEEEECCCCCCHHH
LTSPMRRRPDGTYEEIDWDTAIVEIAEGFKHIRDSYGGDKIFYYGGGGQGNHLGGAYSGA
HHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHH
FLKAIGSKYRSNALAQEKTGEAWIDKNLYGGHTRGEFEYAEVSVFVGKNPWMSQSFPRAR
HHHHHHHHHHHCCHHHHCCCCHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHH
VVLNEIAKDPQRSMIVIDPVNTDTAKMADFHLRVRPGTDAWCLAALAAVLVQENLCDEAF
HHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHH
LAEHVTGTEEVRDVLRTVPIADYARRCGVDEDLIRRAARRVAAAESVAVFEDLGIQQAPN
HHHHCCCHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
STLSSYLNKMLWILTGNFAKRGGQHLHSWMAPLMGGFFGYTPVTGARIIGGLVPSNVIPE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCH
EILTDHPDRFRAMIVESSNPAHSLADSARCKEAFAALELLVVVDVAMTETARLAHYVLPA
HHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ATQFEKVEATFFNLEFPHNTFQLRHPLFEPESGTLPEPEIWARLVRALGVVDDADLQPLR
HHHHHHHEEEEEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
EAAAQGREAYLQAFFAVLANPVLGALAPYVLYETLGPTLPRGLEGAAALWGLAQKAMMTY
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
PQAVARAGHPDGNALFDAILNSPSGVTFTVHDYEDDFALITHADRKIALAIPEMLDEIKG
HHHHHHCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCEEEEHHHHHHHHHHH
LVSRPAQLTTPEFPIVLSAGERRAYTANDILRDPSWRKRDQQGALRISPQDAQQLGVADG
HHCCCCCCCCCCCCEEEECCCCCCEEHHHHHCCCCCCCCCCCCCEEECCHHHHHCCCCCC
ERVRIATAAGAAEATVEISDMMQPGHASLPNGFGLDYTDESGETHIPGVAPNALTSSAWR
CEEEEEEECCCCHHEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DEYAGTPWHKHVPARLETLTAAI
HHCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite; acceptor

Specific reaction: nitrite + acceptor = nitrate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1552862 [H]