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The map label for this gene is greA

Identifier: 169628294

GI number: 169628294

Start: 1216119

End: 1216613

Strand: Reverse

Name: greA

Synonym: MAB_1201c

Alternate gene names: 169628294

Gene position: 1216613-1216119 (Counterclockwise)

Preceding gene: 169628295

Following gene: 169628291

Centisome position: 24.01

GC content: 67.07

Gene sequence:

>495_bases
ATGACCGACACCCAGGTGACCTGGCTGACCCAGGAATCACATGATCGGCTCAAGGCGGAACTCGACCAACTGATCGCCAA
CCGCCCCGTCATCGCCGCCGAGATCAACGAGCGCCGCGAAGAGGGAGACCTGCGCGAGAACGGCGGCTATCACGCCGCCC
GCGAGGAGCAGGGCCAGCAGGAGGCGCGCATCCGTCAGCTGCAGGAACTGCTCAACAACGCCAAGGTGGGCGAGGCGCCC
ACCCAGTCCGGCGTCGCGCTGCCCGGTTCCGTCGTCAAGGTCTACTACGACGGCGACAAGGGCGACACCGAGACCTTCCT
CATCGCCACCCGTCAGGAGGGCGTCAAGGACGGCAAGCTCGAGGTGTACTCCCCCAGCTCACCGCTCGGTGGTGCCCTGA
TCGACGCCAAGGTCGGCGAGACCCGCAGCTACACGGTGCCCAACGGCACCGAGGTGCAGGTGACGCTGGTCAGCGCCGAG
CCGTACCACGAGTGA

Upstream 100 bases:

>100_bases
GCATTTTGGAGAGTCAAACCCGCAGCAATGCACCCGACCCGGCACCCGATAGATGAGCGCCAGTGGACGTTGCTGGCCTG
AGACAAGGAGTACCTGCACC

Downstream 100 bases:

>100_bases
TTCCTTCATAGTCCTTCGATCGAGCGCGAGCCTCACGCGGAAATCTGACCTGAAATCCGCGTGAGGCTCGCACTCGTTGT
GGGCCGGGCGGCATTTCTGA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTDTQVTWLTQESHDRLKAELDQLIANRPVIAAEINERREEGDLRENGGYHAAREEQGQQEARIRQLQELLNNAKVGEAP
TQSGVALPGSVVKVYYDGDKGDTETFLIATRQEGVKDGKLEVYSPSSPLGGALIDAKVGETRSYTVPNGTEVQVTLVSAE
PYHE

Sequences:

>Translated_164_residues
MTDTQVTWLTQESHDRLKAELDQLIANRPVIAAEINERREEGDLRENGGYHAAREEQGQQEARIRQLQELLNNAKVGEAP
TQSGVALPGSVVKVYYDGDKGDTETFLIATRQEGVKDGKLEVYSPSSPLGGALIDAKVGETRSYTVPNGTEVQVTLVSAE
PYHE
>Mature_163_residues
TDTQVTWLTQESHDRLKAELDQLIANRPVIAAEINERREEGDLRENGGYHAAREEQGQQEARIRQLQELLNNAKVGEAPT
QSGVALPGSVVKVYYDGDKGDTETFLIATRQEGVKDGKLEVYSPSSPLGGALIDAKVGETRSYTVPNGTEVQVTLVSAEP
YHE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=160, Percent_Identity=34.375, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_MYCA9 (B1MKJ7)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001701943.1
- ProteinModelPortal:   B1MKJ7
- SMR:   B1MKJ7
- EnsemblBacteria:   EBMYCT00000008736
- GeneID:   5963725
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_1201c
- GeneTree:   EBGT00050000017261
- HOGENOM:   HBG753387
- OMA:   GYHAAKE
- ProtClustDB:   PRK00226
- BioCyc:   MABS561007:MAB_1201C-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18007; Mature: 17876

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTQVTWLTQESHDRLKAELDQLIANRPVIAAEINERREEGDLRENGGYHAAREEQGQQ
CCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCCCCCHHHHCCHH
EARIRQLQELLNNAKVGEAPTQSGVALPGSVVKVYYDGDKGDTETFLIATRQEGVKDGKL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCE
EVYSPSSPLGGALIDAKVGETRSYTVPNGTEVQVTLVSAEPYHE
EEECCCCCCCCEEEECCCCCCCEEECCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
TDTQVTWLTQESHDRLKAELDQLIANRPVIAAEINERREEGDLRENGGYHAAREEQGQQ
CCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCCCCCHHHHCCHH
EARIRQLQELLNNAKVGEAPTQSGVALPGSVVKVYYDGDKGDTETFLIATRQEGVKDGKL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCE
EVYSPSSPLGGALIDAKVGETRSYTVPNGTEVQVTLVSAEPYHE
EEECCCCCCCCEEEECCCCCCCEEECCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA