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The map label for this gene is ksgA [H]

Identifier: 169628224

GI number: 169628224

Start: 1142716

End: 1143621

Strand: Direct

Name: ksgA [H]

Synonym: MAB_1131

Alternate gene names: 169628224

Gene position: 1142716-1143621 (Clockwise)

Preceding gene: 169628223

Following gene: 169628229

Centisome position: 22.55

GC content: 65.23

Gene sequence:

>906_bases
GTGGGGCGCTTGGCCGGTATGCAGCGCGAGAGTTGGTGCCCGCTGACCATCAGGCTGCTCGGCCGTACCGAGATCCGACA
ACTGGCCAAGGATCTCGATTTCCGACCGACGAAGACGCTGGGCCAGAACTTTGTTCATGACGCCAACACCATTCGCCGGA
TCGTGTCGGCCTCGGGTGTGGGTAAGGGCGACCACGTCATCGAGGTCGGGCCCGGGCTCGGCTCGCTGACGCTGGGTCTG
CTCGATAAGGGCGCCGCGGTGACCGCTGTCGAGGTCGATCCCGTGCTGGCGCGGCGCCTTCCGACCACTATCAAGGAACA
CTCGCACAGCGAGTTCAACCGATTCTCGGTGATACACCAGGACGTGCTGAACCTCACGCCCGCCGATATTCAGGGCGAGC
CGACGGCGATGGTGGCGAACCTCCCGTACAACGTCGCGGTGCCCGCGCTCCTGCGAATCCTGACCGAATTCGACTCGGTG
AAAACCATTTTGGTGATGGTTCAGTCCGAGGTCGCCGATCGCCTGACCGCGGAGCCGGGTTCGCGGATCTACGGGGTGCC
CAGCGTCAAGGTGCGCTACTTCGGCAAGGTGAAGCGTCTGGGCACGGTGTCGCCGGCGGTGTTCTGGCCGATCCCGCGGG
TGTACTCGGGACTGGTACGCATCGATCGCCACGCGGATGCGGCATGGCCCTCCGACGCCGGGTTCCGCAAGGAGCTCTTC
GATCTCATCGATATCTCGTTCGCGCAGCGCCGCAAGACATCTCGTAATGCCTTCGCCGAATGGGCGGGCTCGGGTGACGA
GTCGGCGCGTCGGCTGCTGGCGGCCAGCATCGACCCCGCGCGCCGCGGCGAGACGCTGGATGTCGCCGATTTCGCGCGGC
TGCTGGAACGTTCGGCAATCAGCTAA

Upstream 100 bases:

>100_bases
CCTGGCTGGCACACGGCGGCGGCAAGTACGCGCCCCGCGCCGACCTCGCGACGCGCGAGGAGCAGATCGCGATCGCGGAG
AAGACACTGTCGGCGCAGGG

Downstream 100 bases:

>100_bases
TTCCCGCCCGTCATCTCTCGGCGCTGAGTGGCGGGCTATTTGTCGCCGAGATTGGGCGGGTCGTCCATCATCGGGGTCTC
CGCGATGACGGGGTACGCGC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MGRLAGMQRESWCPLTIRLLGRTEIRQLAKDLDFRPTKTLGQNFVHDANTIRRIVSASGVGKGDHVIEVGPGLGSLTLGL
LDKGAAVTAVEVDPVLARRLPTTIKEHSHSEFNRFSVIHQDVLNLTPADIQGEPTAMVANLPYNVAVPALLRILTEFDSV
KTILVMVQSEVADRLTAEPGSRIYGVPSVKVRYFGKVKRLGTVSPAVFWPIPRVYSGLVRIDRHADAAWPSDAGFRKELF
DLIDISFAQRRKTSRNAFAEWAGSGDESARRLLAASIDPARRGETLDVADFARLLERSAIS

Sequences:

>Translated_301_residues
MGRLAGMQRESWCPLTIRLLGRTEIRQLAKDLDFRPTKTLGQNFVHDANTIRRIVSASGVGKGDHVIEVGPGLGSLTLGL
LDKGAAVTAVEVDPVLARRLPTTIKEHSHSEFNRFSVIHQDVLNLTPADIQGEPTAMVANLPYNVAVPALLRILTEFDSV
KTILVMVQSEVADRLTAEPGSRIYGVPSVKVRYFGKVKRLGTVSPAVFWPIPRVYSGLVRIDRHADAAWPSDAGFRKELF
DLIDISFAQRRKTSRNAFAEWAGSGDESARRLLAASIDPARRGETLDVADFARLLERSAIS
>Mature_300_residues
GRLAGMQRESWCPLTIRLLGRTEIRQLAKDLDFRPTKTLGQNFVHDANTIRRIVSASGVGKGDHVIEVGPGLGSLTLGLL
DKGAAVTAVEVDPVLARRLPTTIKEHSHSEFNRFSVIHQDVLNLTPADIQGEPTAMVANLPYNVAVPALLRILTEFDSVK
TILVMVQSEVADRLTAEPGSRIYGVPSVKVRYFGKVKRLGTVSPAVFWPIPRVYSGLVRIDRHADAAWPSDAGFRKELFD
LIDISFAQRRKTSRNAFAEWAGSGDESARRLLAASIDPARRGETLDVADFARLLERSAIS

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=280, Percent_Identity=28.2142857142857, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI156415992, Length=305, Percent_Identity=27.8688524590164, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=32.9501915708812, Blast_Score=134, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI25146882, Length=215, Percent_Identity=32.093023255814, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25141369, Length=302, Percent_Identity=26.158940397351, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=191, Percent_Identity=28.2722513089005, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21358017, Length=284, Percent_Identity=25.7042253521127, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 33059; Mature: 32928

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLAGMQRESWCPLTIRLLGRTEIRQLAKDLDFRPTKTLGQNFVHDANTIRRIVSASGV
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GKGDHVIEVGPGLGSLTLGLLDKGAAVTAVEVDPVLARRLPTTIKEHSHSEFNRFSVIHQ
CCCCCEEEECCCCCHHHHHHHCCCCEEEEEECCHHHHHHCCHHHHHHCCHHHHHHHHHHH
DVLNLTPADIQGEPTAMVANLPYNVAVPALLRILTEFDSVKTILVMVQSEVADRLTAEPG
HHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRIYGVPSVKVRYFGKVKRLGTVSPAVFWPIPRVYSGLVRIDRHADAAWPSDAGFRKELF
CEEECCCCCEEHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
DLIDISFAQRRKTSRNAFAEWAGSGDESARRLLAASIDPARRGETLDVADFARLLERSAI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHCC
S
C
>Mature Secondary Structure 
GRLAGMQRESWCPLTIRLLGRTEIRQLAKDLDFRPTKTLGQNFVHDANTIRRIVSASGV
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GKGDHVIEVGPGLGSLTLGLLDKGAAVTAVEVDPVLARRLPTTIKEHSHSEFNRFSVIHQ
CCCCCEEEECCCCCHHHHHHHCCCCEEEEEECCHHHHHHCCHHHHHHCCHHHHHHHHHHH
DVLNLTPADIQGEPTAMVANLPYNVAVPALLRILTEFDSVKTILVMVQSEVADRLTAEPG
HHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRIYGVPSVKVRYFGKVKRLGTVSPAVFWPIPRVYSGLVRIDRHADAAWPSDAGFRKELF
CEEECCCCCEEHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
DLIDISFAQRRKTSRNAFAEWAGSGDESARRLLAASIDPARRGETLDVADFARLLERSAI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA