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The map label for this gene is pcaF [H]

Identifier: 169628001

GI number: 169628001

Start: 895283

End: 896506

Strand: Direct

Name: pcaF [H]

Synonym: MAB_0902

Alternate gene names: 169628001

Gene position: 895283-896506 (Clockwise)

Preceding gene: 169627997

Following gene: 169628002

Centisome position: 17.67

GC content: 67.65

Gene sequence:

>1224_bases
GTGGTGGAGCAGGTGTTCGTGGTCGACGGAGTGCGCACGGCGCAGGGGCGCTACGGGGGAGCGCTGGCGTCGGTGCGACC
GGATGACTTGGCGGCTCGGGTCATCGCCGAGGTAGTCGCGCGTGCCGGCGTGCCGGCCGATGCGATCGGCGAGGTGATTC
TCGGCGCAGCCAATCAGGCGGGCGAGGACAACCGAAACGTCGCGCGGATGGCTGCGTTACTGGCCGGATTGCCGAACACG
GTGCCCGGCTACACGGTCAACCGGCTGTGCGCGAGTGGGCTGACCGCGATCTCCTCTGCGGCGCAGGCGATTCGGGCGGG
TGAGGCAGATATTGTGGTCGCCGGCGGTGTCGAATCCATGACGCGGGCGCCCTGGGTTATGGCCAAGCCGAGCGCGCCGT
GGGCCCGCCCGGGAGAGGTGGTCGATACATCGCTCGGCTGGCGATTCACCAATCCAAGATTCGCTCAGATGGACGCTGGT
ATCGCCGCCGACGCCGGGCCCGAAACTGTCAAGGTAACCCTGGCGATGGGTGAAACCGCCGAGGAAGTCGCTGTTGCAGA
GGGCATTTCCCGCGAGGACTCAGATGCCTTCGCGCTGCGTAGTCATGTTCGCGCTCTCGCCGCTCAAGACGCGGGCCGGT
TCGTCAAAGAAATCGTTCCCATCGAAACCGTGGACGGAGTCGTCGACACCGACGAGGGGCCACGCCGGCAAACCACGTTG
GCCAAACTTGCGGCGTTGCGGCCGGTCTTTAGGGCCGGCGGCATCGTGACGGCGGGGTCTTCGTCACCGCTGTCGGACGG
TGCTGCCGCAGTCGTGTTGGCCGGCGAACGCGCCGTGCGGCAACACGGACTGACGGTGCGTGCGCGGGTGGTTGCTGCCG
CCAGTGCGGGCGTCGCACCCAATCTGATGGGGTTGGGGCCCGTGCCCGCGGTTCGCAAGGTGGTGGACCGAGTCGGGTGG
CGAGTCGATGCCATCGACGCTACGGAAATCAACGAGGCGTTCGCCGCCCAGACCCTGGCGGTGCTGCGGCGCCTGGGCTT
GGACGAGGACACGGTCAACGCCGACGGCGGCGCCATCGCACTGGGTCATCCGTTGGGGTGTTCGGGTGCGCGGCTCATCG
TCACCTTGCTGAACCGGCTGGAGCGTGAGGGCGCATCTCGCGGGCTGGCGACCTTGTGTGTGGGAGTCGGACAGGGTGTC
GCGATATTGATCGAGGCACCGTGA

Upstream 100 bases:

>100_bases
CACCGCGTGTGCGCTGGTGGCGTTTCCACTCCCAACGGTCCCGGCCTCTCTAGACAACCGACCGAACGATCGGTTATTAA
TGTTTTGTTGAGAGGAGTGA

Downstream 100 bases:

>100_bases
CCGAATATCAAACATTGTGTGTCACCACAGAAGACGACCGGGTGATCGTTGAGCTGCATCGTCCCGAGCAGCGCAACGCG
ATCAATGCCGCGATGGTCGC

Product: beta-ketoadipyl-CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MVEQVFVVDGVRTAQGRYGGALASVRPDDLAARVIAEVVARAGVPADAIGEVILGAANQAGEDNRNVARMAALLAGLPNT
VPGYTVNRLCASGLTAISSAAQAIRAGEADIVVAGGVESMTRAPWVMAKPSAPWARPGEVVDTSLGWRFTNPRFAQMDAG
IAADAGPETVKVTLAMGETAEEVAVAEGISREDSDAFALRSHVRALAAQDAGRFVKEIVPIETVDGVVDTDEGPRRQTTL
AKLAALRPVFRAGGIVTAGSSSPLSDGAAAVVLAGERAVRQHGLTVRARVVAAASAGVAPNLMGLGPVPAVRKVVDRVGW
RVDAIDATEINEAFAAQTLAVLRRLGLDEDTVNADGGAIALGHPLGCSGARLIVTLLNRLEREGASRGLATLCVGVGQGV
AILIEAP

Sequences:

>Translated_407_residues
MVEQVFVVDGVRTAQGRYGGALASVRPDDLAARVIAEVVARAGVPADAIGEVILGAANQAGEDNRNVARMAALLAGLPNT
VPGYTVNRLCASGLTAISSAAQAIRAGEADIVVAGGVESMTRAPWVMAKPSAPWARPGEVVDTSLGWRFTNPRFAQMDAG
IAADAGPETVKVTLAMGETAEEVAVAEGISREDSDAFALRSHVRALAAQDAGRFVKEIVPIETVDGVVDTDEGPRRQTTL
AKLAALRPVFRAGGIVTAGSSSPLSDGAAAVVLAGERAVRQHGLTVRARVVAAASAGVAPNLMGLGPVPAVRKVVDRVGW
RVDAIDATEINEAFAAQTLAVLRRLGLDEDTVNADGGAIALGHPLGCSGARLIVTLLNRLEREGASRGLATLCVGVGQGV
AILIEAP
>Mature_407_residues
MVEQVFVVDGVRTAQGRYGGALASVRPDDLAARVIAEVVARAGVPADAIGEVILGAANQAGEDNRNVARMAALLAGLPNT
VPGYTVNRLCASGLTAISSAAQAIRAGEADIVVAGGVESMTRAPWVMAKPSAPWARPGEVVDTSLGWRFTNPRFAQMDAG
IAADAGPETVKVTLAMGETAEEVAVAEGISREDSDAFALRSHVRALAAQDAGRFVKEIVPIETVDGVVDTDEGPRRQTTL
AKLAALRPVFRAGGIVTAGSSSPLSDGAAAVVLAGERAVRQHGLTVRARVVAAASAGVAPNLMGLGPVPAVRKVVDRVGW
RVDAIDATEINEAFAAQTLAVLRRLGLDEDTVNADGGAIALGHPLGCSGARLIVTLLNRLEREGASRGLATLCVGVGQGV
AILIEAP

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=409, Percent_Identity=41.320293398533, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI167614485, Length=410, Percent_Identity=39.5121951219512, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI4501853, Length=414, Percent_Identity=39.1304347826087, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI4557237, Length=410, Percent_Identity=36.0975609756098, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=32.0185614849188, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI194353979, Length=405, Percent_Identity=31.1111111111111, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1787663, Length=412, Percent_Identity=52.9126213592233, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI87082165, Length=408, Percent_Identity=45.343137254902, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1788554, Length=410, Percent_Identity=44.8780487804878, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI48994986, Length=415, Percent_Identity=43.6144578313253, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788683, Length=430, Percent_Identity=32.093023255814, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI133906874, Length=406, Percent_Identity=41.6256157635468, Blast_Score=280, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI17535921, Length=410, Percent_Identity=35.609756097561, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI25147385, Length=407, Percent_Identity=33.9066339066339, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17551802, Length=440, Percent_Identity=29.3181818181818, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=29.4554455445545, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322031, Length=409, Percent_Identity=37.6528117359413, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6325229, Length=420, Percent_Identity=37.8571428571429, Blast_Score=219, Evalue=7e-58,
Organism=Drosophila melanogaster, GI24655093, Length=405, Percent_Identity=44.1975308641975, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI17648125, Length=409, Percent_Identity=39.119804400978, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24640423, Length=410, Percent_Identity=35.8536585365854, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=33.2558139534884, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41638; Mature: 41638

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEQVFVVDGVRTAQGRYGGALASVRPDDLAARVIAEVVARAGVPADAIGEVILGAANQA
CCCEEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
GEDNRNVARMAALLAGLPNTVPGYTVNRLCASGLTAISSAAQAIRAGEADIVVAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHH
TRAPWVMAKPSAPWARPGEVVDTSLGWRFTNPRFAQMDAGIAADAGPETVKVTLAMGETA
HCCCEEEECCCCCCCCCCCEEECCCCCEECCCCHHHHCCCCCCCCCCCEEEEEEEECCCH
EEVAVAEGISREDSDAFALRSHVRALAAQDAGRFVKEIVPIETVDGVVDTDEGPRRQTTL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEECCCCCCHHHHH
AKLAALRPVFRAGGIVTAGSSSPLSDGAAAVVLAGERAVRQHGLTVRARVVAAASAGVAP
HHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECHHHHHHCCCEEEEHEEEHHHCCCCC
NLMGLGPVPAVRKVVDRVGWRVDAIDATEINEAFAAQTLAVLRRLGLDEDTVNADGGAIA
CCCCCCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
LGHPLGCSGARLIVTLLNRLEREGASRGLATLCVGVGQGVAILIEAP
ECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MVEQVFVVDGVRTAQGRYGGALASVRPDDLAARVIAEVVARAGVPADAIGEVILGAANQA
CCCEEEEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
GEDNRNVARMAALLAGLPNTVPGYTVNRLCASGLTAISSAAQAIRAGEADIVVAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHH
TRAPWVMAKPSAPWARPGEVVDTSLGWRFTNPRFAQMDAGIAADAGPETVKVTLAMGETA
HCCCEEEECCCCCCCCCCCEEECCCCCEECCCCHHHHCCCCCCCCCCCEEEEEEEECCCH
EEVAVAEGISREDSDAFALRSHVRALAAQDAGRFVKEIVPIETVDGVVDTDEGPRRQTTL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEECCCCCCHHHHH
AKLAALRPVFRAGGIVTAGSSSPLSDGAAAVVLAGERAVRQHGLTVRARVVAAASAGVAP
HHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECHHHHHHCCCEEEEHEEEHHHCCCCC
NLMGLGPVPAVRKVVDRVGWRVDAIDATEINEAFAAQTLAVLRRLGLDEDTVNADGGAIA
CCCCCCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
LGHPLGCSGARLIVTLLNRLEREGASRGLATLCVGVGQGVAILIEAP
ECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]