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The map label for this gene is mhpE [C]

Identifier: 169627729

GI number: 169627729

Start: 625350

End: 626429

Strand: Direct

Name: mhpE [C]

Synonym: MAB_0626

Alternate gene names: 169627729

Gene position: 625350-626429 (Clockwise)

Preceding gene: 169627728

Following gene: 169627731

Centisome position: 12.34

GC content: 63.33

Gene sequence:

>1080_bases
ATGGGTTCGGACACCAGCACTCAGCTCTTGGGCACCGACGGCATCTTCTTCGACGCCGCATGGGACGTGCGGGTCACCGA
TACGTCGTTGCGTGATGGCTCGCACCACAAGCGGCATCAATTCACCGCGGATGAGGTGCGGGCTATCGTGGCGGCGCTGG
ACGGCGCCGGCGTCCCCGTCATCGAGGTGACTCACGGCGACGGCCTGGGCGGATCCTCGTTCAACTACGGATTCTCGAAA
ACCCCTGAGCAGGAACTGATCAAGCTTGCCGCCCAGACCGCGACGAATGCCAAGATCGCCTTCCTGATGCTGCCGGGCGT
AGGCACCAAGGAAGACATCATCGAGGCGCAGGGCAACGGCGGGTCGATCTGTCGCATAGCCACTCACTGCACCGAGGCCG
ACGTCTCGATCCAACACTTCGGGCTCGCCCGTGAGCGCGGTCTGGAAACCGTTGGCTTCCTGATGATGTCGCACACCATC
TCGCCGGAGAAATTGGCCAAGCAGGCACGGATAATGGCAGACGCCGGCTGCCAGTGCGTATACGTGGTGGATTCCGCGGG
TGCGTTGGTCCTGGACGGAGTGGCCGACCGGGTCGCCGCGGTAGTGGCGGAATTGGGGTCGGATGCTCAAGTGGGTTTTC
ATGGGCATGAGAACCTCGGGCTTGGTGTCGCCAACTCCATCGAGGCGGTGCGCGCCGGCGCCAAGCAGATTGACGGCAGC
ACAAGGCGATTCGGCGCCGGTGCAGGCAATGCTCCGGTGGAGGCGCTTATCGGTGTGTTCGACAAGATCGGTGTGAAGAC
GGGCATCGACTTCTTCGATATCGCCGACGCCGCCGAAGAGGTTGTGGCGCCCGCGATGCCCGCGGAATGCCTGCTGGATC
GCAATGCGCTGATCATGGGTTATAGCGGTGTGTACTCCAGCTTCCTCAAGCACGCCATCCGTCAGGGTGAGCGGTATGGG
GTACCCGCGCATCAGCTGCTGCACCGTGCCGGCGAACGCAAGCTGATCGGTGGGCAGGAGGACCAGCTCATCGACATCGC
GCTGGAGATCCAGCGGGAGAATGCCGCGAACGGGCAGTAG

Upstream 100 bases:

>100_bases
TTGCCGCCGTATGCCGGAAACCTGGACATCATGACCGCGGCCGCGACCAAGGTCGGCGAGGAAATCGCCAAAGAACTTCT
ATCTGCAAAGGTTTCGTGAA

Downstream 100 bases:

>100_bases
GCGCTCCCGGATCGACGACCTCGGCGACTGTACCCAGGTCGGTGCCGCAGACCTCGGTCGCCGGGGTCGAGCGTGGTCCG
GCACCGTAGAATTGCCTCAG

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 1; 4-hydroxy-2-keto-pentanoic acid aldolase 1; 4-hydroxy-2-oxopentanoate aldolase 1

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MGSDTSTQLLGTDGIFFDAAWDVRVTDTSLRDGSHHKRHQFTADEVRAIVAALDGAGVPVIEVTHGDGLGGSSFNYGFSK
TPEQELIKLAAQTATNAKIAFLMLPGVGTKEDIIEAQGNGGSICRIATHCTEADVSIQHFGLARERGLETVGFLMMSHTI
SPEKLAKQARIMADAGCQCVYVVDSAGALVLDGVADRVAAVVAELGSDAQVGFHGHENLGLGVANSIEAVRAGAKQIDGS
TRRFGAGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEEVVAPAMPAECLLDRNALIMGYSGVYSSFLKHAIRQGERYG
VPAHQLLHRAGERKLIGGQEDQLIDIALEIQRENAANGQ

Sequences:

>Translated_359_residues
MGSDTSTQLLGTDGIFFDAAWDVRVTDTSLRDGSHHKRHQFTADEVRAIVAALDGAGVPVIEVTHGDGLGGSSFNYGFSK
TPEQELIKLAAQTATNAKIAFLMLPGVGTKEDIIEAQGNGGSICRIATHCTEADVSIQHFGLARERGLETVGFLMMSHTI
SPEKLAKQARIMADAGCQCVYVVDSAGALVLDGVADRVAAVVAELGSDAQVGFHGHENLGLGVANSIEAVRAGAKQIDGS
TRRFGAGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEEVVAPAMPAECLLDRNALIMGYSGVYSSFLKHAIRQGERYG
VPAHQLLHRAGERKLIGGQEDQLIDIALEIQRENAANGQ
>Mature_358_residues
GSDTSTQLLGTDGIFFDAAWDVRVTDTSLRDGSHHKRHQFTADEVRAIVAALDGAGVPVIEVTHGDGLGGSSFNYGFSKT
PEQELIKLAAQTATNAKIAFLMLPGVGTKEDIIEAQGNGGSICRIATHCTEADVSIQHFGLARERGLETVGFLMMSHTIS
PEKLAKQARIMADAGCQCVYVVDSAGALVLDGVADRVAAVVAELGSDAQVGFHGHENLGLGVANSIEAVRAGAKQIDGST
RRFGAGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEEVVAPAMPAECLLDRNALIMGYSGVYSSFLKHAIRQGERYGV
PAHQLLHRAGERKLIGGQEDQLIDIALEIQRENAANGQ

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=327, Percent_Identity=50.7645259938838, Blast_Score=321, Evalue=5e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA1_MYCA9 (B1MH40)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001701378.1
- ProteinModelPortal:   B1MH40
- SMR:   B1MH40
- EnsemblBacteria:   EBMYCT00000008559
- GeneID:   5963162
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_0626
- GeneTree:   EBGT00050000015758
- HOGENOM:   HBG300122
- OMA:   ELGSDAQ
- ProtClustDB:   PRK08195
- BioCyc:   MABS561007:MAB_0626-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 37778; Mature: 37647

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 185-185 BINDING 214-214 BINDING 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDTSTQLLGTDGIFFDAAWDVRVTDTSLRDGSHHKRHQFTADEVRAIVAALDGAGVPV
CCCCCCCEEEECCCEEEEEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCE
IEVTHGDGLGGSSFNYGFSKTPEQELIKLAAQTATNAKIAFLMLPGVGTKEDIIEAQGNG
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHEECCCC
GSICRIATHCTEADVSIQHFGLARERGLETVGFLMMSHTISPEKLAKQARIMADAGCQCV
CCEEEEEECCCCCCCCHHHHCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
YVVDSAGALVLDGVADRVAAVVAELGSDAQVGFHGHENLGLGVANSIEAVRAGAKQIDGS
EEECCCCCEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
TRRFGAGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEEVVAPAMPAECLLDRNALIMG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEE
YSGVYSSFLKHAIRQGERYGVPAHQLLHRAGERKLIGGQEDQLIDIALEIQRENAANGQ
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
GSDTSTQLLGTDGIFFDAAWDVRVTDTSLRDGSHHKRHQFTADEVRAIVAALDGAGVPV
CCCCCCEEEECCCEEEEEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCE
IEVTHGDGLGGSSFNYGFSKTPEQELIKLAAQTATNAKIAFLMLPGVGTKEDIIEAQGNG
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHEECCCC
GSICRIATHCTEADVSIQHFGLARERGLETVGFLMMSHTISPEKLAKQARIMADAGCQCV
CCEEEEEECCCCCCCCHHHHCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
YVVDSAGALVLDGVADRVAAVVAELGSDAQVGFHGHENLGLGVANSIEAVRAGAKQIDGS
EEECCCCCEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
TRRFGAGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEEVVAPAMPAECLLDRNALIMG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEE
YSGVYSSFLKHAIRQGERYGVPAHQLLHRAGERKLIGGQEDQLIDIALEIQRENAANGQ
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA