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The map label for this gene is lysS [H]

Identifier: 169627648

GI number: 169627648

Start: 544546

End: 546048

Strand: Direct

Name: lysS [H]

Synonym: MAB_0544

Alternate gene names: 169627648

Gene position: 544546-546048 (Clockwise)

Preceding gene: 169627647

Following gene: 169627649

Centisome position: 10.75

GC content: 59.28

Gene sequence:

>1503_bases
GTGACTGATCCTGACGCGCAGACCAGCTTGCCGGAACAATTTCGCATCCGGCAGGCCAAGCGCGAGAAGCTGCTTGCCGA
AGGCGTTCAACCGTATCCGGTGGAGGTGCCCAGAACCCATAGCCTCAAGCAGATTCGAGATGCCTACACGGATCTGGAAA
CCGATACCAGCACCGGCGACCAGGTAGGTGTCTCGGGCCGGGTCATCTTCTCCCGCAACACCGGCAAGCTGTGTTTCGCG
ACGCTTCAGGAGGGTGACGGCACCTCTCTACAGGTGATGATCAGCCTCGCCGGCGTCGGCGAGCAGTCGCTCGCCGACTG
GAAGACCGATGTGGACCTAGGCGACATCGTCTTTGTGCACGGAGAGGTCATCAGCTCGCGCCGGGGCGAGTTGAGCGTGA
TGGCGGATCGCTGGCAGATGGCATCGAAGTCCCTCCGGCCTCTGCCCGTTGCTCACAAGGAGATGTCCGAAGAGACCAGA
GTGCGCCAGCGCTACGTGGATCTCATCGTGCGACCCGAGGCGCGCCAGATCGCGCGACAGCGTGTCGCGGTGATGCGGGC
TATTCGTGACGCTATGCACCGCCGTGACTTCTTGGAGTTGGAGACGCCGATCTTGCAGACCCTCGCCGGAGGTGCTGCCG
CACGCCCCTTCATTACGCATTCGAACGCGCTTGATATCGACCTGTATCTGCGTATTGCGCCGGAACTTTTCCTGAAAAAG
GCACTCGTCGGGGGTTTCGACAAGATTTTCGAACTCAACCGGGTATTCCGAAATGAAGGCGCGGATTCCACACATTCCCC
CGAATTCGTGATGTTGGAAACATACGAGACCTACGGCACATACGACACCGCCGCGACAATGATCCGTGAGCTCATCCAAG
AGGTAGCCGACGACGCAATGGGAACCAGGCAGGTGACGTTGCCCGATGGGTCGACCTACGACTTGGACGGCGAGTGGACG
ACCTTGGAAATGTATCCGTCGTTGTCAGAAGCTCTCGGGGAGGAGATCACGCCCGATACGCCGGTTGCCGAGCTATGGAA
GATCGCAGATCGGGTTGGCGCCGAGATTCCTACCGACCGCGGTTATGGGCACGGAAAACTGATCGAGGAACTATGGGAAC
ACCAGGTCGGTGACAAAATGTACGCTCCGACTTTTGTCAGGGATTTCCCAGTTGAGACGTCCCCGTTGACCCGTCAGCAC
CGGAGTATTGCGGGTGTCACCGAGAAGTGGGATTTGTATGTCCGTGGCTTTGAATTGGCCACCGGGTACTCGGAGCTCAT
TGACCCGGTCATTCAACGCGAAAGGTTCGTTGACCAGGCCCGATTGGCGGCCGCCGGTGATGATGAGGCGATGAGTCTGG
ACGAGGATTTCCTCGGCGCGATGGAGCACGCGATGCCTCCTGCCGCAGGAACGGGGATGGGCATTGACCGGTTGTTGATG
GCGCTTACGGGGCTGGGAATTCGAGAAACGATCTTGTTCCCGATTGTGCGACCCACACTCTGA

Upstream 100 bases:

>100_bases
GCGGACCAGCGCGGCAAGGCACGCGCGACCAGGTAGAGATCCATGCGTCAGATCGGCAGCGCAGTTCAGGTGCGGAGCAC
TACCGCTAAGCTGCCGAGAC

Downstream 100 bases:

>100_bases
TCTACGCGTCCGAATTTTTGTCACACTTTTCAACTATGAGTGTAGTGAAACGCTTGCCTTGCGCGGAACACGCGCAGCCT
TGCGCGTATGTTGAACTTTG

Product: lysyl-tRNA synthetase (LysS)

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTDPDAQTSLPEQFRIRQAKREKLLAEGVQPYPVEVPRTHSLKQIRDAYTDLETDTSTGDQVGVSGRVIFSRNTGKLCFA
TLQEGDGTSLQVMISLAGVGEQSLADWKTDVDLGDIVFVHGEVISSRRGELSVMADRWQMASKSLRPLPVAHKEMSEETR
VRQRYVDLIVRPEARQIARQRVAVMRAIRDAMHRRDFLELETPILQTLAGGAAARPFITHSNALDIDLYLRIAPELFLKK
ALVGGFDKIFELNRVFRNEGADSTHSPEFVMLETYETYGTYDTAATMIRELIQEVADDAMGTRQVTLPDGSTYDLDGEWT
TLEMYPSLSEALGEEITPDTPVAELWKIADRVGAEIPTDRGYGHGKLIEELWEHQVGDKMYAPTFVRDFPVETSPLTRQH
RSIAGVTEKWDLYVRGFELATGYSELIDPVIQRERFVDQARLAAAGDDEAMSLDEDFLGAMEHAMPPAAGTGMGIDRLLM
ALTGLGIRETILFPIVRPTL

Sequences:

>Translated_500_residues
MTDPDAQTSLPEQFRIRQAKREKLLAEGVQPYPVEVPRTHSLKQIRDAYTDLETDTSTGDQVGVSGRVIFSRNTGKLCFA
TLQEGDGTSLQVMISLAGVGEQSLADWKTDVDLGDIVFVHGEVISSRRGELSVMADRWQMASKSLRPLPVAHKEMSEETR
VRQRYVDLIVRPEARQIARQRVAVMRAIRDAMHRRDFLELETPILQTLAGGAAARPFITHSNALDIDLYLRIAPELFLKK
ALVGGFDKIFELNRVFRNEGADSTHSPEFVMLETYETYGTYDTAATMIRELIQEVADDAMGTRQVTLPDGSTYDLDGEWT
TLEMYPSLSEALGEEITPDTPVAELWKIADRVGAEIPTDRGYGHGKLIEELWEHQVGDKMYAPTFVRDFPVETSPLTRQH
RSIAGVTEKWDLYVRGFELATGYSELIDPVIQRERFVDQARLAAAGDDEAMSLDEDFLGAMEHAMPPAAGTGMGIDRLLM
ALTGLGIRETILFPIVRPTL
>Mature_499_residues
TDPDAQTSLPEQFRIRQAKREKLLAEGVQPYPVEVPRTHSLKQIRDAYTDLETDTSTGDQVGVSGRVIFSRNTGKLCFAT
LQEGDGTSLQVMISLAGVGEQSLADWKTDVDLGDIVFVHGEVISSRRGELSVMADRWQMASKSLRPLPVAHKEMSEETRV
RQRYVDLIVRPEARQIARQRVAVMRAIRDAMHRRDFLELETPILQTLAGGAAARPFITHSNALDIDLYLRIAPELFLKKA
LVGGFDKIFELNRVFRNEGADSTHSPEFVMLETYETYGTYDTAATMIRELIQEVADDAMGTRQVTLPDGSTYDLDGEWTT
LEMYPSLSEALGEEITPDTPVAELWKIADRVGAEIPTDRGYGHGKLIEELWEHQVGDKMYAPTFVRDFPVETSPLTRQHR
SIAGVTEKWDLYVRGFELATGYSELIDPVIQRERFVDQARLAAAGDDEAMSLDEDFLGAMEHAMPPAAGTGMGIDRLLMA
LTGLGIRETILFPIVRPTL

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=514, Percent_Identity=37.15953307393, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI194272210, Length=514, Percent_Identity=37.15953307393, Blast_Score=305, Evalue=7e-83,
Organism=Escherichia coli, GI1789256, Length=509, Percent_Identity=37.721021611002, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=37.625754527163, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI87082379, Length=336, Percent_Identity=29.7619047619048, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788173, Length=246, Percent_Identity=27.2357723577236, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=503, Percent_Identity=36.182902584493, Blast_Score=300, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17535927, Length=503, Percent_Identity=36.182902584493, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI71994340, Length=500, Percent_Identity=36.2, Blast_Score=297, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI17551876, Length=502, Percent_Identity=22.9083665338645, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=502, Percent_Identity=35.8565737051793, Blast_Score=276, Evalue=6e-75,
Organism=Saccharomyces cerevisiae, GI6324256, Length=462, Percent_Identity=28.7878787878788, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24640849, Length=517, Percent_Identity=36.7504835589942, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24640851, Length=510, Percent_Identity=37.0588235294118, Blast_Score=305, Evalue=3e-83,
Organism=Drosophila melanogaster, GI17136276, Length=468, Percent_Identity=23.9316239316239, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 55890; Mature: 55759

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPDAQTSLPEQFRIRQAKREKLLAEGVQPYPVEVPRTHSLKQIRDAYTDLETDTSTGD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
QVGVSGRVIFSRNTGKLCFATLQEGDGTSLQVMISLAGVGEQSLADWKTDVDLGDIVFVH
CCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCEEEEE
GEVISSRRGELSVMADRWQMASKSLRPLPVAHKEMSEETRVRQRYVDLIVRPEARQIARQ
CHHHHCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RVAVMRAIRDAMHRRDFLELETPILQTLAGGAAARPFITHSNALDIDLYLRIAPELFLKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEEHHHHHHHHHH
ALVGGFDKIFELNRVFRNEGADSTHSPEFVMLETYETYGTYDTAATMIRELIQEVADDAM
HHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHCC
GTRQVTLPDGSTYDLDGEWTTLEMYPSLSEALGEEITPDTPVAELWKIADRVGAEIPTDR
CCEEEECCCCCEEECCCCEEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
GYGHGKLIEELWEHQVGDKMYAPTFVRDFPVETSPLTRQHRSIAGVTEKWDLYVRGFELA
CCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEHHH
TGYSELIDPVIQRERFVDQARLAAAGDDEAMSLDEDFLGAMEHAMPPAAGTGMGIDRLLM
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
ALTGLGIRETILFPIVRPTL
HHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDPDAQTSLPEQFRIRQAKREKLLAEGVQPYPVEVPRTHSLKQIRDAYTDLETDTSTGD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
QVGVSGRVIFSRNTGKLCFATLQEGDGTSLQVMISLAGVGEQSLADWKTDVDLGDIVFVH
CCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCEEEEE
GEVISSRRGELSVMADRWQMASKSLRPLPVAHKEMSEETRVRQRYVDLIVRPEARQIARQ
CHHHHCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RVAVMRAIRDAMHRRDFLELETPILQTLAGGAAARPFITHSNALDIDLYLRIAPELFLKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEEHHHHHHHHHH
ALVGGFDKIFELNRVFRNEGADSTHSPEFVMLETYETYGTYDTAATMIRELIQEVADDAM
HHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHCC
GTRQVTLPDGSTYDLDGEWTTLEMYPSLSEALGEEITPDTPVAELWKIADRVGAEIPTDR
CCEEEECCCCCEEECCCCEEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
GYGHGKLIEELWEHQVGDKMYAPTFVRDFPVETSPLTRQHRSIAGVTEKWDLYVRGFELA
CCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEHHH
TGYSELIDPVIQRERFVDQARLAAAGDDEAMSLDEDFLGAMEHAMPPAAGTGMGIDRLLM
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
ALTGLGIRETILFPIVRPTL
HHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA