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The map label for this gene is oppA [H]

Identifier: 169627530

GI number: 169627530

Start: 425984

End: 427603

Strand: Direct

Name: oppA [H]

Synonym: MAB_0426

Alternate gene names: 169627530

Gene position: 425984-427603 (Clockwise)

Preceding gene: 169627528

Following gene: 169627531

Centisome position: 8.41

GC content: 61.17

Gene sequence:

>1620_bases
ATGAGGTTGCGCCGACTGGCCATCGTGTTGCTTGTCGGCATTGTCGCCGTCCTGCCCGCCGGCTGCGGTGACCTCGTCAC
TCCGGCGCCTCCCGGCTACTTCAGCATGTACATCACCGAACCTGAGCACACCTTGGTGCCGGGAAACACCACTGAGAATC
AGGGTGGTCGGATCCTGCGCTCGCTATTCACCGCGCTGGTCGAGTTCAACAACGAGACCGCTGCGGTGGAGTACACCGGG
GTGGCCGAATCCATCACCAGTCACGACAACGTCAACTGGACGATCAAGCTCAAACCCGGGTGGACGTTCCACAACGGGGA
ACCGGTCACCGCCCAGTCCTACGTCGATGCCTGGAACTACACCGCCCTGAGCACCAATGCCCAGGGGTCCTCCAGCTTCC
TCGCCAATGTTGACGGCTTCGATGATCTCCAGGGTGACCCGGCACACAAGATCGCGCCGCGGGCCACACAGATGCGCGGG
TTGCACGTCGTGGATGACGTCACCTTCACCGTGCGCCTCAACACGCCGTTCGCGATCTATCCGATGACCTTGGGGTACAC
AGCGTTTCTGCCCCTGCCCAAGGCGTTCTTCCGGGACCCGGTCAAGTTCGGGGTGCACCCCATCGGCAATGGCCCGTTCA
AGGCCGACGGTGATTGGGTGCGTGGTGTCGGATTCACCCTCAGCCGGTACGACCAGTACGCCGGGGAGAAAAAGGCCAAG
GCCAAGGGTCTGATTTGGCGGGTGTACACCGAGGGCCTGACCGCCTACACCGATATGCAGGCCGGGGCACTGGACATTCA
ACTGGACCTTCCCGACGCCGCCTACGAGAACGCCAAGGCCGAATTCGGGTCGGCGTATCTGGAACGACCCCGCCCGGATA
TCACGTCGTTGGGATTCCCGATGTACGACCCGCGATATCGCGATGTGCGTGTGCGGCAGGCTATTTCCATGGCCATCGAC
AGGGAGGCGATCACCCAGGTAATCTTCTCCGGCACTCGCACGCCCGCCACCTCGTACGGCTCGGCGGTGGTCTACGGGTA
CCGCAAGGGGGCGTGTGGCAAGCTTTGCGAACTGCACGTGGATGAGGCCAACAAGCTGCTCGACGAGGCCCATTTCGATC
GGAACAAGCCCATTGAGCTGTGGTACAGCTCGTCGAGCACGGGGGCACTGTCCTGGGTACAGGCCATCGGCAATCAGCTG
CAGTCCAATCTGAAAGTTCCGTACCTGCTCAAAAGCCTGCCCTGGTCACAGTTCCTGCCACTGCAGGACGGCAAGGGGAT
GACGGGGCCGTTCCGGTCCGGTTGGGTCATGGATTATCCGTCGATCTATAACTTCCTGGAGTCGCTGTACGGCACGGTAG
CCCTGCCTCCGAACGGTTCCAACTATGTGTTCTACAGCAATCCGGCATTCGATGAGCTGCTGCGCCAAGGCAACAACGAG
CCGACGGTAGAACTCGCGACCAAGGCGTATCAGAAGGCGGAAGACCTGTTGTTCAAGGATCTTCCGGCCGCGCCGCTCTT
CTATGAGGTCAACCAGGCGGTGCATTCCAAGCGGGTGCGCAATGTCAAGATCGGCATCCAGGACTGTATTGAGTACGCAG
ACGTGGAGGTGGTGCAGTGA

Upstream 100 bases:

>100_bases
CGTTCAGAAGCGGGCGCGCTGGTGTGTCGATGGAGAGACATCGGCGCGTTACCCGCCTATGGTTCGTCCTACCTATTCAG
CTACCGAGGAAAGGGCGAGG

Downstream 100 bases:

>100_bases
CCACCGTGTTGGGCAACGTTCGACAGTTCTGGCAGAGCGCCGGTCTCTTCCGCTACATCGCCAAGCGGCTGCTGCTCGCG
ATACCCGTTCTCATCGGGAC

Product: peptide ABC transporter DppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MRLRRLAIVLLVGIVAVLPAGCGDLVTPAPPGYFSMYITEPEHTLVPGNTTENQGGRILRSLFTALVEFNNETAAVEYTG
VAESITSHDNVNWTIKLKPGWTFHNGEPVTAQSYVDAWNYTALSTNAQGSSSFLANVDGFDDLQGDPAHKIAPRATQMRG
LHVVDDVTFTVRLNTPFAIYPMTLGYTAFLPLPKAFFRDPVKFGVHPIGNGPFKADGDWVRGVGFTLSRYDQYAGEKKAK
AKGLIWRVYTEGLTAYTDMQAGALDIQLDLPDAAYENAKAEFGSAYLERPRPDITSLGFPMYDPRYRDVRVRQAISMAID
REAITQVIFSGTRTPATSYGSAVVYGYRKGACGKLCELHVDEANKLLDEAHFDRNKPIELWYSSSSTGALSWVQAIGNQL
QSNLKVPYLLKSLPWSQFLPLQDGKGMTGPFRSGWVMDYPSIYNFLESLYGTVALPPNGSNYVFYSNPAFDELLRQGNNE
PTVELATKAYQKAEDLLFKDLPAAPLFYEVNQAVHSKRVRNVKIGIQDCIEYADVEVVQ

Sequences:

>Translated_539_residues
MRLRRLAIVLLVGIVAVLPAGCGDLVTPAPPGYFSMYITEPEHTLVPGNTTENQGGRILRSLFTALVEFNNETAAVEYTG
VAESITSHDNVNWTIKLKPGWTFHNGEPVTAQSYVDAWNYTALSTNAQGSSSFLANVDGFDDLQGDPAHKIAPRATQMRG
LHVVDDVTFTVRLNTPFAIYPMTLGYTAFLPLPKAFFRDPVKFGVHPIGNGPFKADGDWVRGVGFTLSRYDQYAGEKKAK
AKGLIWRVYTEGLTAYTDMQAGALDIQLDLPDAAYENAKAEFGSAYLERPRPDITSLGFPMYDPRYRDVRVRQAISMAID
REAITQVIFSGTRTPATSYGSAVVYGYRKGACGKLCELHVDEANKLLDEAHFDRNKPIELWYSSSSTGALSWVQAIGNQL
QSNLKVPYLLKSLPWSQFLPLQDGKGMTGPFRSGWVMDYPSIYNFLESLYGTVALPPNGSNYVFYSNPAFDELLRQGNNE
PTVELATKAYQKAEDLLFKDLPAAPLFYEVNQAVHSKRVRNVKIGIQDCIEYADVEVVQ
>Mature_539_residues
MRLRRLAIVLLVGIVAVLPAGCGDLVTPAPPGYFSMYITEPEHTLVPGNTTENQGGRILRSLFTALVEFNNETAAVEYTG
VAESITSHDNVNWTIKLKPGWTFHNGEPVTAQSYVDAWNYTALSTNAQGSSSFLANVDGFDDLQGDPAHKIAPRATQMRG
LHVVDDVTFTVRLNTPFAIYPMTLGYTAFLPLPKAFFRDPVKFGVHPIGNGPFKADGDWVRGVGFTLSRYDQYAGEKKAK
AKGLIWRVYTEGLTAYTDMQAGALDIQLDLPDAAYENAKAEFGSAYLERPRPDITSLGFPMYDPRYRDVRVRQAISMAID
REAITQVIFSGTRTPATSYGSAVVYGYRKGACGKLCELHVDEANKLLDEAHFDRNKPIELWYSSSSTGALSWVQAIGNQL
QSNLKVPYLLKSLPWSQFLPLQDGKGMTGPFRSGWVMDYPSIYNFLESLYGTVALPPNGSNYVFYSNPAFDELLRQGNNE
PTVELATKAYQKAEDLLFKDLPAAPLFYEVNQAVHSKRVRNVKIGIQDCIEYADVEVVQ

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=530, Percent_Identity=26.0377358490566, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87081878, Length=466, Percent_Identity=25.3218884120172, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789397, Length=517, Percent_Identity=23.21083172147, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787052, Length=563, Percent_Identity=23.2682060390764, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789966, Length=467, Percent_Identity=21.1991434689507, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59730; Mature: 59730

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRRLAIVLLVGIVAVLPAGCGDLVTPAPPGYFSMYITEPEHTLVPGNTTENQGGRILR
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCCCCHHHHH
SLFTALVEFNNETAAVEYTGVAESITSHDNVNWTIKLKPGWTFHNGEPVTAQSYVDAWNY
HHHHHHHHCCCCEEEEEECCHHHHHHCCCCCEEEEEECCCEEECCCCCCCHHHHHCCCCE
TALSTNAQGSSSFLANVDGFDDLQGDPAHKIAPRATQMRGLHVVDDVTFTVRLNTPFAIY
EEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHCCEEEEECEEEEEEECCCEEEE
PMTLGYTAFLPLPKAFFRDPVKFGVHPIGNGPFKADGDWVRGVGFTLSRYDQYAGEKKAK
EECCCEEEECCCCHHHHCCCHHHCCEECCCCCCCCCCCEEECCCCHHHHHHHHCCCHHHH
AKGLIWRVYTEGLTAYTDMQAGALDIQLDLPDAAYENAKAEFGSAYLERPRPDITSLGFP
HCCEEEEEECCCCCEEECCCCCEEEEEEECCCHHHHCCHHHHHHHHHHCCCCCHHHCCCC
MYDPRYRDVRVRQAISMAIDREAITQVIFSGTRTPATSYGSAVVYGYRKGACGKLCELHV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHEEEH
DEANKLLDEAHFDRNKPIELWYSSSSTGALSWVQAIGNQLQSNLKVPYLLKSLPWSQFLP
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCHHHCCC
LQDGKGMTGPFRSGWVMDYPSIYNFLESLYGTVALPPNGSNYVFYSNPAFDELLRQGNNE
CCCCCCCCCCCCCCCEECCHHHHHHHHHHHCEEEECCCCCEEEEECCCCHHHHHHCCCCC
PTVELATKAYQKAEDLLFKDLPAAPLFYEVNQAVHSKRVRNVKIGIQDCIEYADVEVVQ
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEECHHHHHHHCCCEEEC
>Mature Secondary Structure
MRLRRLAIVLLVGIVAVLPAGCGDLVTPAPPGYFSMYITEPEHTLVPGNTTENQGGRILR
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCCCCHHHHH
SLFTALVEFNNETAAVEYTGVAESITSHDNVNWTIKLKPGWTFHNGEPVTAQSYVDAWNY
HHHHHHHHCCCCEEEEEECCHHHHHHCCCCCEEEEEECCCEEECCCCCCCHHHHHCCCCE
TALSTNAQGSSSFLANVDGFDDLQGDPAHKIAPRATQMRGLHVVDDVTFTVRLNTPFAIY
EEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHCCEEEEECEEEEEEECCCEEEE
PMTLGYTAFLPLPKAFFRDPVKFGVHPIGNGPFKADGDWVRGVGFTLSRYDQYAGEKKAK
EECCCEEEECCCCHHHHCCCHHHCCEECCCCCCCCCCCEEECCCCHHHHHHHHCCCHHHH
AKGLIWRVYTEGLTAYTDMQAGALDIQLDLPDAAYENAKAEFGSAYLERPRPDITSLGFP
HCCEEEEEECCCCCEEECCCCCEEEEEEECCCHHHHCCHHHHHHHHHHCCCCCHHHCCCC
MYDPRYRDVRVRQAISMAIDREAITQVIFSGTRTPATSYGSAVVYGYRKGACGKLCELHV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHEEEH
DEANKLLDEAHFDRNKPIELWYSSSSTGALSWVQAIGNQLQSNLKVPYLLKSLPWSQFLP
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCHHHCCC
LQDGKGMTGPFRSGWVMDYPSIYNFLESLYGTVALPPNGSNYVFYSNPAFDELLRQGNNE
CCCCCCCCCCCCCCCEECCHHHHHHHHHHHCEEEECCCCCEEEEECCCCHHHHHHCCCCC
PTVELATKAYQKAEDLLFKDLPAAPLFYEVNQAVHSKRVRNVKIGIQDCIEYADVEVVQ
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEECHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]