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The map label for this gene is nth [H]

Identifier: 169627522

GI number: 169627522

Start: 417805

End: 418602

Strand: Direct

Name: nth [H]

Synonym: MAB_0418

Alternate gene names: 169627522

Gene position: 417805-418602 (Clockwise)

Preceding gene: 169627519

Following gene: 169627523

Centisome position: 8.25

GC content: 64.54

Gene sequence:

>798_bases
GTGCCAACAACCGGATCAGCCGCTAAGCCCAGAGCGAAATCGTCGGTGAAGGCCCCAGCGCCGGTCCGTGCTTGGAAGCC
CGAGACGCACACAGGATTGGTCCGTCGCGCCCGGCGGATGAATCGCACTCTGGCGCAGGCGTTTCCGCACGTCTACTGTG
AGCTCGACTTTACCAACCCGCTGGAGCTGGCGGTGGCGACCATCCTGTCGGCCCAATGCACCGATGTCCGCGTCAACATG
GTGACGCCCGCGTTGTTCGCCAAATATCGCACCGCCGAGGACTACGCGGGTGCCAACCGCGCCGAGCTCGAAGAGATGAT
TCGCACCACCGGCTTCTATCGGAACAAGGCCAACTCGATCATGGGGTTGGGCACACAGCTGGTGGAGCGCTTCGGCGGAG
AGATCCCGCCGCGGTTGAAGGATCTGGTGACGCTGCCCGGCATCGGGCGCAAGACCGCAAATGTGATCCTGGGCAATGCC
TTTGATATTCCGGGCATCACCGTCGACACGCATTTCGGTCGGTTGGTGCGCCGCTGGCGCTGGACCGAGGAAGAAGACCC
GGTGAAGGTCGAGCACCTCGTCGGTGAGCTCATCGAGCGCAAGGAGTGGACGTTACTCAGCCACCGGGTGATCTTTCACG
GCCGTCGCGTGTGCCATGCGCGCAAACCCGCCTGCGGAGTCTGCGTGCTGGCCAAGGACTGTCCGTCCTATGGCCTGGGC
CCGACGGACCCGGAGGTGGCTGCGCCACTGGTGAAGGGCCCGGAGACCGAGCACCTGCTGGCGTTGGCCGGGTTGTGA

Upstream 100 bases:

>100_bases
CGGCGAGGTCACGAAAAACAACCGGTTTGTCCTGCGCACGGGGCGAAATGGCGTGCGGGCCGACCCAGCCCTTGTCCTGG
CCCGCGGCTACCCTGGGATT

Downstream 100 bases:

>100_bases
CGTGGCCCGACTCAACACCGGGGCCCGGTGGACGATCGCCGGGGTGGTGCTGGTCGCGGCGCTGATCGCCGTCATGTTGT
CGCAGCAACACGATCAGCAG

Product: endonuclease III protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPTTGSAAKPRAKSSVKAPAPVRAWKPETHTGLVRRARRMNRTLAQAFPHVYCELDFTNPLELAVATILSAQCTDVRVNM
VTPALFAKYRTAEDYAGANRAELEEMIRTTGFYRNKANSIMGLGTQLVERFGGEIPPRLKDLVTLPGIGRKTANVILGNA
FDIPGITVDTHFGRLVRRWRWTEEEDPVKVEHLVGELIERKEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSYGLG
PTDPEVAAPLVKGPETEHLLALAGL

Sequences:

>Translated_265_residues
MPTTGSAAKPRAKSSVKAPAPVRAWKPETHTGLVRRARRMNRTLAQAFPHVYCELDFTNPLELAVATILSAQCTDVRVNM
VTPALFAKYRTAEDYAGANRAELEEMIRTTGFYRNKANSIMGLGTQLVERFGGEIPPRLKDLVTLPGIGRKTANVILGNA
FDIPGITVDTHFGRLVRRWRWTEEEDPVKVEHLVGELIERKEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSYGLG
PTDPEVAAPLVKGPETEHLLALAGL
>Mature_264_residues
PTTGSAAKPRAKSSVKAPAPVRAWKPETHTGLVRRARRMNRTLAQAFPHVYCELDFTNPLELAVATILSAQCTDVRVNMV
TPALFAKYRTAEDYAGANRAELEEMIRTTGFYRNKANSIMGLGTQLVERFGGEIPPRLKDLVTLPGIGRKTANVILGNAF
DIPGITVDTHFGRLVRRWRWTEEEDPVKVEHLVGELIERKEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSYGLGP
TDPEVAAPLVKGPETEHLLALAGL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=228, Percent_Identity=26.7543859649123, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1787920, Length=203, Percent_Identity=36.4532019704434, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17554540, Length=136, Percent_Identity=33.8235294117647, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=29.4736842105263, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319304, Length=153, Percent_Identity=27.4509803921569, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550361, Length=199, Percent_Identity=28.1407035175879, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 29293; Mature: 29162

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00063 ALDOKETO_REDUCTASE_3 ; PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTGSAAKPRAKSSVKAPAPVRAWKPETHTGLVRRARRMNRTLAQAFPHVYCELDFTNP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCH
LELAVATILSAQCTDVRVNMVTPALFAKYRTAEDYAGANRAELEEMIRTTGFYRNKANSI
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHCCHH
MGLGTQLVERFGGEIPPRLKDLVTLPGIGRKTANVILGNAFDIPGITVDTHFGRLVRRWR
HHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHEEECCCCCCCCEEECHHHHHHHHHHC
WTEEEDPVKVEHLVGELIERKEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSYGLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
PTDPEVAAPLVKGPETEHLLALAGL
CCCHHHHHHHHCCCCHHHEEEECCC
>Mature Secondary Structure 
PTTGSAAKPRAKSSVKAPAPVRAWKPETHTGLVRRARRMNRTLAQAFPHVYCELDFTNP
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCH
LELAVATILSAQCTDVRVNMVTPALFAKYRTAEDYAGANRAELEEMIRTTGFYRNKANSI
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHCCHH
MGLGTQLVERFGGEIPPRLKDLVTLPGIGRKTANVILGNAFDIPGITVDTHFGRLVRRWR
HHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHEEECCCCCCCCEEECHHHHHHHHHHC
WTEEEDPVKVEHLVGELIERKEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSYGLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
PTDPEVAAPLVKGPETEHLLALAGL
CCCHHHHHHHHCCCCHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]