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The map label for this gene is opuCB [H]

Identifier: 169627369

GI number: 169627369

Start: 265748

End: 266500

Strand: Direct

Name: opuCB [H]

Synonym: MAB_0264

Alternate gene names: 169627369

Gene position: 265748-266500 (Clockwise)

Preceding gene: 169627368

Following gene: 169627370

Centisome position: 5.24

GC content: 68.26

Gene sequence:

>753_bases
GTGAATTTTCTCTCCAACGCACTCGGCTACATATTCACCGCCTCCCATTGGGCGGGCAGTAGCGGCTTGGGTGTGCGGAT
CGGCGAGCACCTGGAGTACACGGCGCTCGCGGTGCTGGCCGCTGTCGTGATCGCGGTGCCGCTGGGTTTGTTCATCGGGC
ACACCGGTCGCGGCATCTTCCTGGTGGTGAGCGCGGTGAACGCGCTGCGGGCACTGCCGACGCTGGGCATGTTGCTGTTG
GGTGTGCTGCTGTGGGGGCTGGGCCTGGTGCCGCCGACCGTTGCGTTGCTGCTGCTGGGTGTGCCACCGCTGTTGGCGGG
CACCTACTCGGGCATCGCGAACGTGGACCGCACGGTAGTGGAGGCGGCGCGCGCCATGGGGATGACCGAACGTCAGGTGC
TGTTCGGTGTCGAGGTGCCTAATGCGCTCCCGCTCATTCTGTCCGGGCTCCGCACGGCGACGCTGCAAGTCGTGGCGACC
GCGACGGTAGCGGCCTACGCCAGCCTGGGTGGGCTCGGCCGGTATCTCATCGACGGCATCAAGGTCCGTCAGTTTCATCT
GGCGCTGGTGGGCGCCATCATGGTCACCGCGCTGGCGCTGCTGCTCGATGCGGTGCTCGCCCTCGCGGTGTGGTTATCGG
CCCCGGGGACCGGACGGCTGCGGCCGGTGCCCGACCTTCCGGATCGCGAAGGACACGCCGTGTACGCGGATAGCGGTGTG
TCCGCGGGCGCAATGCCGCGCTCGCCGGGTTAG

Upstream 100 bases:

>100_bases
TCGCGATCTTTCTGCTGGCGGTCATCGTGGATTCACTGCTGGTGGTTCTGGGGCGCGTGGCGACACCCTGGGCCAGGGCG
ACGAAAACCGCTGGTGCCAC

Downstream 100 bases:

>100_bases
CCCGGCCGGTAGGGGCTCAAGGACACCCCAATTTGCGCGGTCGCCTACGGTAGACGGGTGAATACCCCCGAGGCGACTGG
GCCTGCCGTGCTGACCTGGC

Product: osmoprotectant ABC transporter ProZ

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNFLSNALGYIFTASHWAGSSGLGVRIGEHLEYTALAVLAAVVIAVPLGLFIGHTGRGIFLVVSAVNALRALPTLGMLLL
GVLLWGLGLVPPTVALLLLGVPPLLAGTYSGIANVDRTVVEAARAMGMTERQVLFGVEVPNALPLILSGLRTATLQVVAT
ATVAAYASLGGLGRYLIDGIKVRQFHLALVGAIMVTALALLLDAVLALAVWLSAPGTGRLRPVPDLPDREGHAVYADSGV
SAGAMPRSPG

Sequences:

>Translated_250_residues
MNFLSNALGYIFTASHWAGSSGLGVRIGEHLEYTALAVLAAVVIAVPLGLFIGHTGRGIFLVVSAVNALRALPTLGMLLL
GVLLWGLGLVPPTVALLLLGVPPLLAGTYSGIANVDRTVVEAARAMGMTERQVLFGVEVPNALPLILSGLRTATLQVVAT
ATVAAYASLGGLGRYLIDGIKVRQFHLALVGAIMVTALALLLDAVLALAVWLSAPGTGRLRPVPDLPDREGHAVYADSGV
SAGAMPRSPG
>Mature_250_residues
MNFLSNALGYIFTASHWAGSSGLGVRIGEHLEYTALAVLAAVVIAVPLGLFIGHTGRGIFLVVSAVNALRALPTLGMLLL
GVLLWGLGLVPPTVALLLLGVPPLLAGTYSGIANVDRTVVEAARAMGMTERQVLFGVEVPNALPLILSGLRTATLQVVAT
ATVAAYASLGGLGRYLIDGIKVRQFHLALVGAIMVTALALLLDAVLALAVWLSAPGTGRLRPVPDLPDREGHAVYADSGV
SAGAMPRSPG

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1174

COG function: function code E; ABC-type proline/glycine betaine transport systems, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=163, Percent_Identity=31.2883435582822, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788451, Length=200, Percent_Identity=32.5, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788449, Length=178, Percent_Identity=32.0224719101124, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25680; Mature: 25680

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLSNALGYIFTASHWAGSSGLGVRIGEHLEYTALAVLAAVVIAVPLGLFIGHTGRGIF
CCHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LVVSAVNALRALPTLGMLLLGVLLWGLGLVPPTVALLLLGVPPLLAGTYSGIANVDRTVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EAARAMGMTERQVLFGVEVPNALPLILSGLRTATLQVVATATVAAYASLGGLGRYLIDGI
HHHHHHCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVRQFHLALVGAIMVTALALLLDAVLALAVWLSAPGTGRLRPVPDLPDREGHAVYADSGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCC
SAGAMPRSPG
CCCCCCCCCC
>Mature Secondary Structure
MNFLSNALGYIFTASHWAGSSGLGVRIGEHLEYTALAVLAAVVIAVPLGLFIGHTGRGIF
CCHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LVVSAVNALRALPTLGMLLLGVLLWGLGLVPPTVALLLLGVPPLLAGTYSGIANVDRTVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EAARAMGMTERQVLFGVEVPNALPLILSGLRTATLQVVATATVAAYASLGGLGRYLIDGI
HHHHHHCCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVRQFHLALVGAIMVTALALLLDAVLALAVWLSAPGTGRLRPVPDLPDREGHAVYADSGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCC
SAGAMPRSPG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377 [H]