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The map label for this gene is rfbE [H]

Identifier: 169627308

GI number: 169627308

Start: 212809

End: 213600

Strand: Reverse

Name: rfbE [H]

Synonym: MAB_0203c

Alternate gene names: 169627308

Gene position: 213600-212809 (Counterclockwise)

Preceding gene: 169627309

Following gene: 169627307

Centisome position: 4.22

GC content: 62.37

Gene sequence:

>792_bases
ATGGTCAGTATCCAAACTCACCAGGCGTGGGTGGAATTCCCCATATTCGACGCCAAGTCACGCTCGCTCAAGAAGGCGTT
CCTGGGCAAGGCGGGCGGCGCCATCGGGCGCAACGAGTCCAATGTCGTGGTCATCGAGGCCCTGCGTGACATCACGCTGT
CCCTCAAGGAGGGCGACCGCGTTGGGCTCGTCGGCCACAACGGCGCGGGTAAATCGACTCTGCTGCGGCTCCTTTCGGGA
ATCTACGAACCCACGCGGGGCAGCTCCTACATCAAGGGCCGCGTCGCACCCGTGTTCGACCTGGGCGTGGGTATGGATCC
GGAAATCTCCGGGTACGAGAACATCATCATCCGCGGCATGTTCCTGGGACAGACCCGCAAGCAAATGCAAGCCAAGGTCG
ACGAGATCGCCGACTTCACCGAGCTCGGCGAGTACCTGTCCATGCCGCTGCGTACGTATTCCACCGGTATGCGCGTGCGC
CTGGCCATGGGCGTGGTCACCAGCATCGACCCCGAGATCCTGCTACTCGATGAGGGCATCGGTGCCGTCGACGCAGAGTT
CATGAAGAAGGCGCGAATCCGGTTGCAGCAGTTGGTAGAGCGCTCCGGCATGCTGGTCTTCGCCAGCCACTCCAATGAGT
TCTTGGCCCGGCTGTGCAACACGGCGATGTGGATCGACCACGGCACCATCAAGATGAGCGGCGGCATCGAGGACGTGGTC
CGTGCCTACGAAGGCGATGAGGCCGGAGATCACGTGGCCCAGATCCTTGCCGAAGACGCGCACGATGGCTAG

Upstream 100 bases:

>100_bases
ACAGCTGCAGCAGATGCGCAAAGGAGCTCTGCCCCCAGGGCTTTCGGTTCCCGCACAGGGCGGCGGCAGCGGCACCGGCC
AGTACCTGTAAGTCCGAAAC

Downstream 100 bases:

>100_bases
TCCCTCACGGAACAACGTTGTCGCCGTCGTCGTCACCTACCGCCGCGCGGCGGAATTGGCGCAATCGCTGCAGATAGTCG
CCGCGCAGACCGTCGCCCCG

Product: o-antigen/lipopolysaccharide transport ATP-binding protein ABC transporter RfbE

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MVSIQTHQAWVEFPIFDAKSRSLKKAFLGKAGGAIGRNESNVVVIEALRDITLSLKEGDRVGLVGHNGAGKSTLLRLLSG
IYEPTRGSSYIKGRVAPVFDLGVGMDPEISGYENIIIRGMFLGQTRKQMQAKVDEIADFTELGEYLSMPLRTYSTGMRVR
LAMGVVTSIDPEILLLDEGIGAVDAEFMKKARIRLQQLVERSGMLVFASHSNEFLARLCNTAMWIDHGTIKMSGGIEDVV
RAYEGDEAGDHVAQILAEDAHDG

Sequences:

>Translated_263_residues
MVSIQTHQAWVEFPIFDAKSRSLKKAFLGKAGGAIGRNESNVVVIEALRDITLSLKEGDRVGLVGHNGAGKSTLLRLLSG
IYEPTRGSSYIKGRVAPVFDLGVGMDPEISGYENIIIRGMFLGQTRKQMQAKVDEIADFTELGEYLSMPLRTYSTGMRVR
LAMGVVTSIDPEILLLDEGIGAVDAEFMKKARIRLQQLVERSGMLVFASHSNEFLARLCNTAMWIDHGTIKMSGGIEDVV
RAYEGDEAGDHVAQILAEDAHDG
>Mature_263_residues
MVSIQTHQAWVEFPIFDAKSRSLKKAFLGKAGGAIGRNESNVVVIEALRDITLSLKEGDRVGLVGHNGAGKSTLLRLLSG
IYEPTRGSSYIKGRVAPVFDLGVGMDPEISGYENIIIRGMFLGQTRKQMQAKVDEIADFTELGEYLSMPLRTYSTGMRVR
LAMGVVTSIDPEILLLDEGIGAVDAEFMKKARIRLQQLVERSGMLVFASHSNEFLARLCNTAMWIDHGTIKMSGGIEDVV
RAYEGDEAGDHVAQILAEDAHDG

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=215, Percent_Identity=25.5813953488372, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27477115, Length=174, Percent_Identity=25.8620689655172, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI6005701, Length=185, Percent_Identity=24.8648648648649, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI116734710, Length=178, Percent_Identity=28.0898876404494, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI31657092, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI27436953, Length=185, Percent_Identity=28.6486486486486, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790467, Length=197, Percent_Identity=28.9340101522843, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87082267, Length=167, Percent_Identity=31.7365269461078, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788761, Length=213, Percent_Identity=27.6995305164319, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081709, Length=214, Percent_Identity=25.2336448598131, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI48995001, Length=182, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081791, Length=178, Percent_Identity=26.4044943820225, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787712, Length=177, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081782, Length=217, Percent_Identity=29.0322580645161, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788450, Length=207, Percent_Identity=26.0869565217391, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789032, Length=225, Percent_Identity=25.3333333333333, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787089, Length=208, Percent_Identity=27.4038461538462, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788225, Length=211, Percent_Identity=25.5924170616114, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787758, Length=178, Percent_Identity=30.3370786516854, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1790525, Length=216, Percent_Identity=25, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787041, Length=202, Percent_Identity=27.2277227722772, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787029, Length=211, Percent_Identity=24.1706161137441, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI48994943, Length=154, Percent_Identity=29.8701298701299, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787547, Length=240, Percent_Identity=22.0833333333333, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1789873, Length=211, Percent_Identity=26.5402843601896, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=213, Percent_Identity=25.8215962441315, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=180, Percent_Identity=30, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221500365, Length=176, Percent_Identity=28.4090909090909, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI116007184, Length=176, Percent_Identity=28.4090909090909, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28573571, Length=207, Percent_Identity=28.0193236714976, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI28573573, Length=207, Percent_Identity=28.0193236714976, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 28729; Mature: 28729

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIQTHQAWVEFPIFDAKSRSLKKAFLGKAGGAIGRNESNVVVIEALRDITLSLKEGDR
CCEECCCHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHEEEECCCCE
VGLVGHNGAGKSTLLRLLSGIYEPTRGSSYIKGRVAPVFDLGVGMDPEISGYENIIIRGM
EEEEECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCEEEEEEE
FLGQTRKQMQAKVDEIADFTELGEYLSMPLRTYSTGMRVRLAMGVVTSIDPEILLLDEGI
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEHHHHCCCCCEEEEECCC
GAVDAEFMKKARIRLQQLVERSGMLVFASHSNEFLARLCNTAMWIDHGTIKMSGGIEDVV
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHEEEECCEEEECCCHHHHH
RAYEGDEAGDHVAQILAEDAHDG
HHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVSIQTHQAWVEFPIFDAKSRSLKKAFLGKAGGAIGRNESNVVVIEALRDITLSLKEGDR
CCEECCCHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHEEEECCCCE
VGLVGHNGAGKSTLLRLLSGIYEPTRGSSYIKGRVAPVFDLGVGMDPEISGYENIIIRGM
EEEEECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCEEEEEEE
FLGQTRKQMQAKVDEIADFTELGEYLSMPLRTYSTGMRVRLAMGVVTSIDPEILLLDEGI
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEHHHHCCCCCEEEEECCC
GAVDAEFMKKARIRLQQLVERSGMLVFASHSNEFLARLCNTAMWIDHGTIKMSGGIEDVV
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHEEEECCEEEECCCHHHHH
RAYEGDEAGDHVAQILAEDAHDG
HHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]