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The map label for this gene is phoA [H]

Identifier: 169627192

GI number: 169627192

Start: 83095

End: 84654

Strand: Reverse

Name: phoA [H]

Synonym: MAB_0087c

Alternate gene names: 169627192

Gene position: 84654-83095 (Counterclockwise)

Preceding gene: 169627193

Following gene: 169627190

Centisome position: 1.67

GC content: 65.83

Gene sequence:

>1560_bases
ATGCGCATCGCTCACGCAGCTACCGCCGTCACATCCGCACTCACCCTCGCGCTCGCTCCGGTGGCTGCCGCCGCGCCACC
ATCGGATGCGCCCGAGGTCCCCGTGGGCGACATCGCCAAGACAGGCGGCGCCAACCGCACCACGGGCGGGCGCGACGGCG
ACAAGACAGCCGATCTGCGCGATTCGGTGCGGGGGCGCAACGCCAAGAACGTCATCCTGCTGATCGGCGACGGCATGGGC
ACATCGGAGATCACCTCGGCGCGCAACTATCAGTACGGCGCCGCCGGGACACTACCGGGGCTGGACGCATTGCCGATCAC
CGGCGAGTACACCACGTTCGCACTGACCAAGGACGGGCCGGACAAGGGCAAGCCGGACTATGTCACCGACTCGGCGGCCT
CCGGCACCGGATGGGCCACCGGCACCAAGACCTATAACGGCGCCATTTCGGTCGATCTCGAAGGCAAGCCGCTGGATACG
ATCCTGGAGATCGTGCAGAGGCAGGGTTTCGCCACCGGAAACGTCACCACCGCGGAGCTGCAGGATGCTACGCCGGCCGT
GCTCACCGCACATGTCACCGATCGGGACTGCAAGGGCCCCAAAGAGACCCTGGCGAGGTGCGCCGGGAACGCCGTCGACA
ACGGCGGCAGGGGATCCATCTCCGAACAGCTGGTGAACACCCGCGCCGATATCAGCCTGGGTGGTGGCGGCAAGTACTTT
GCGGAGACCATCACCGCCGGTGAGCACAAGGGCAAAACCTCGCTGGAGGTCGCCAAGGCTGCGGGATACCAATTACCCAC
CACAGCAGGCGATCTGAACAAGGTGAACAATCTGGACAATCCGGTGCTGGGCATCTTCGGCACGGGAAACCTGCCGGTGC
AGTGGGACAAGGTACCGGCCGCGGTACCCAACGGCGGCAAGCTGCCCCCAACCGTCTGCAGGCAAAATCCGGACCTGCCC
AGGGACCAACCTCAGCTCTCCGCGATGACCACCAAAGCCATTGATCTACTGGTCAACTCGAAGAAGGGCAAGGACAAGGG
CTTCTTCCTGCAGGTCGAGTCCGCGTCGATCGACAAACAGGATCACGCGGCCAATATCTGCGGACAGATCGGCGAGACCA
TCGCCCTCGACGAGGCCGTCGCCGCCGCCGTCGACTTCGCCCGCCAGGACCGCAACACCCTGGTGATCGTGACCTCCGAT
CACGGGCATTCCTCGCTCATCGTGCCCGATGACTTCAATAGCCCCACAACCCTGTCCGTCGCGGTGAAGACCGCCGACGG
CGCCACCATGCGGATCATGTATCCCACTGCGCCGGAAGGGGAGTCACAGACCCACACCGGTACCCAGGTCCGCATCGCCG
CATACGGCCCGGGTGCCGCGAACGTTTCCGGACTCACCGATCAGACCGACAACTTCAACACGATCGTGGGTGCACTACTC
GGACCCAAGAGCCCCGACGCCGCAAGCGGCCCGTCCTGGTGGGCGGCCGGGGGGATCGCGGTGGTGGCGGCCGCCCTGGG
TGCCGCGGCCGCGTTCCTCGTGGGTAAGCGCCAGACCTAG

Upstream 100 bases:

>100_bases
GTTTCGCGCGGCGAAGGACGCCTGGTTCTGGACCTGCCCGCCGAATAGCCCACTGCGGAGGGTTTACCCGGCGTTCATCG
GTTCCCGGCAAACTGGCCCG

Downstream 100 bases:

>100_bases
CGCCCCACGCGCGAGCAGACACGCATGACAACAACCTCTGGATGGGCGCGCAATACGTGTCTGCTCGCCGACAACGCTAG
GCCACCAACCGCTTCGGATT

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MRIAHAATAVTSALTLALAPVAAAAPPSDAPEVPVGDIAKTGGANRTTGGRDGDKTADLRDSVRGRNAKNVILLIGDGMG
TSEITSARNYQYGAAGTLPGLDALPITGEYTTFALTKDGPDKGKPDYVTDSAASGTGWATGTKTYNGAISVDLEGKPLDT
ILEIVQRQGFATGNVTTAELQDATPAVLTAHVTDRDCKGPKETLARCAGNAVDNGGRGSISEQLVNTRADISLGGGGKYF
AETITAGEHKGKTSLEVAKAAGYQLPTTAGDLNKVNNLDNPVLGIFGTGNLPVQWDKVPAAVPNGGKLPPTVCRQNPDLP
RDQPQLSAMTTKAIDLLVNSKKGKDKGFFLQVESASIDKQDHAANICGQIGETIALDEAVAAAVDFARQDRNTLVIVTSD
HGHSSLIVPDDFNSPTTLSVAVKTADGATMRIMYPTAPEGESQTHTGTQVRIAAYGPGAANVSGLTDQTDNFNTIVGALL
GPKSPDAASGPSWWAAGGIAVVAAALGAAAAFLVGKRQT

Sequences:

>Translated_519_residues
MRIAHAATAVTSALTLALAPVAAAAPPSDAPEVPVGDIAKTGGANRTTGGRDGDKTADLRDSVRGRNAKNVILLIGDGMG
TSEITSARNYQYGAAGTLPGLDALPITGEYTTFALTKDGPDKGKPDYVTDSAASGTGWATGTKTYNGAISVDLEGKPLDT
ILEIVQRQGFATGNVTTAELQDATPAVLTAHVTDRDCKGPKETLARCAGNAVDNGGRGSISEQLVNTRADISLGGGGKYF
AETITAGEHKGKTSLEVAKAAGYQLPTTAGDLNKVNNLDNPVLGIFGTGNLPVQWDKVPAAVPNGGKLPPTVCRQNPDLP
RDQPQLSAMTTKAIDLLVNSKKGKDKGFFLQVESASIDKQDHAANICGQIGETIALDEAVAAAVDFARQDRNTLVIVTSD
HGHSSLIVPDDFNSPTTLSVAVKTADGATMRIMYPTAPEGESQTHTGTQVRIAAYGPGAANVSGLTDQTDNFNTIVGALL
GPKSPDAASGPSWWAAGGIAVVAAALGAAAAFLVGKRQT
>Mature_519_residues
MRIAHAATAVTSALTLALAPVAAAAPPSDAPEVPVGDIAKTGGANRTTGGRDGDKTADLRDSVRGRNAKNVILLIGDGMG
TSEITSARNYQYGAAGTLPGLDALPITGEYTTFALTKDGPDKGKPDYVTDSAASGTGWATGTKTYNGAISVDLEGKPLDT
ILEIVQRQGFATGNVTTAELQDATPAVLTAHVTDRDCKGPKETLARCAGNAVDNGGRGSISEQLVNTRADISLGGGGKYF
AETITAGEHKGKTSLEVAKAAGYQLPTTAGDLNKVNNLDNPVLGIFGTGNLPVQWDKVPAAVPNGGKLPPTVCRQNPDLP
RDQPQLSAMTTKAIDLLVNSKKGKDKGFFLQVESASIDKQDHAANICGQIGETIALDEAVAAAVDFARQDRNTLVIVTSD
HGHSSLIVPDDFNSPTTLSVAVKTADGATMRIMYPTAPEGESQTHTGTQVRIAAYGPGAANVSGLTDQTDNFNTIVGALL
GPKSPDAASGPSWWAAGGIAVVAAALGAAAAFLVGKRQT

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=377, Percent_Identity=27.3209549071618, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI48994877, Length=479, Percent_Identity=56.3674321503132, Blast_Score=481, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6320689, Length=349, Percent_Identity=28.080229226361, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24585213, Length=410, Percent_Identity=31.7073170731707, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24651554, Length=509, Percent_Identity=27.5049115913556, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21355981, Length=361, Percent_Identity=28.2548476454294, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19921912, Length=474, Percent_Identity=28.0590717299578, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21355149, Length=391, Percent_Identity=28.9002557544757, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859923, Length=399, Percent_Identity=26.3157894736842, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355151, Length=467, Percent_Identity=26.7665952890792, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI85725200, Length=468, Percent_Identity=26.2820512820513, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI85815833, Length=468, Percent_Identity=26.2820512820513, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24651556, Length=496, Percent_Identity=26.8145161290323, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24657827, Length=470, Percent_Identity=27.2340425531915, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21358067, Length=473, Percent_Identity=25.369978858351, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 53161; Mature: 53161

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAHAATAVTSALTLALAPVAAAAPPSDAPEVPVGDIAKTGGANRTTGGRDGDKTADLR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHH
DSVRGRNAKNVILLIGDGMGTSEITSARNYQYGAAGTLPGLDALPITGEYTTFALTKDGP
HHHCCCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
DKGKPDYVTDSAASGTGWATGTKTYNGAISVDLEGKPLDTILEIVQRQGFATGNVTTAEL
CCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEC
QDATPAVLTAHVTDRDCKGPKETLARCAGNAVDNGGRGSISEQLVNTRADISLGGGGKYF
CCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEECCCCHHH
AETITAGEHKGKTSLEVAKAAGYQLPTTAGDLNKVNNLDNPVLGIFGTGNLPVQWDKVPA
HHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCEEECCCCC
AVPNGGKLPPTVCRQNPDLPRDQPQLSAMTTKAIDLLVNSKKGKDKGFFLQVESASIDKQ
CCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCCCH
DHAANICGQIGETIALDEAVAAAVDFARQDRNTLVIVTSDHGHSSLIVPDDFNSPTTLSV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCCEEEEE
AVKTADGATMRIMYPTAPEGESQTHTGTQVRIAAYGPGAANVSGLTDQTDNFNTIVGALL
EEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
GPKSPDAASGPSWWAAGGIAVVAAALGAAAAFLVGKRQT
CCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRIAHAATAVTSALTLALAPVAAAAPPSDAPEVPVGDIAKTGGANRTTGGRDGDKTADLR
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHH
DSVRGRNAKNVILLIGDGMGTSEITSARNYQYGAAGTLPGLDALPITGEYTTFALTKDGP
HHHCCCCCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
DKGKPDYVTDSAASGTGWATGTKTYNGAISVDLEGKPLDTILEIVQRQGFATGNVTTAEL
CCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEC
QDATPAVLTAHVTDRDCKGPKETLARCAGNAVDNGGRGSISEQLVNTRADISLGGGGKYF
CCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEECCCCHHH
AETITAGEHKGKTSLEVAKAAGYQLPTTAGDLNKVNNLDNPVLGIFGTGNLPVQWDKVPA
HHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCEEECCCCC
AVPNGGKLPPTVCRQNPDLPRDQPQLSAMTTKAIDLLVNSKKGKDKGFFLQVESASIDKQ
CCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCCCH
DHAANICGQIGETIALDEAVAAAVDFARQDRNTLVIVTSDHGHSSLIVPDDFNSPTTLSV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCCEEEEE
AVKTADGATMRIMYPTAPEGESQTHTGTQVRIAAYGPGAANVSGLTDQTDNFNTIVGALL
EEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
GPKSPDAASGPSWWAAGGIAVVAAALGAAAAFLVGKRQT
CCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]