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The map label for this gene is fhlA [H]

Identifier: 16766165

GI number: 16766165

Start: 3003702

End: 3005780

Strand: Direct

Name: fhlA [H]

Synonym: STM2859

Alternate gene names: 16766165

Gene position: 3003702-3005780 (Clockwise)

Preceding gene: 39546350

Following gene: 16766167

Centisome position: 61.84

GC content: 54.93

Gene sequence:

>2079_bases
ATGTCGTATACACCCATGAGCGATCTTGGACAGCAAGGCTTGTTCGATATCACTCGTACCTTATTGCAGCAGCCCGATTT
GGCGTCGCTCAGTGAAGCGCTTTCGCAACTGGTAAAGCGTTCAGCGCTCGCCGACAGCGCCGGCATTGTGCTATGGCAGG
CGCAGTCGCAACGCGCACAATATTATGCGACGCGAGAAAATGGCCGTCCTGTTGAATATGAAGATGAAACCGTACTGGCG
CACGGCCCGGTACGTCGCATCCTTTCTCGCCCCGATGCATTGCACTGTAATTTCCATGAGTTTACCGAAACCTGGCCGCA
GCTTGCCGCGAGCGGCCTCTACCCTGAATTTGGTCATTACTGTCTGCTTCCTTTAGCCGCGGAGGGCCGTATTTTCGGCG
GCTGCGAATTCATCCGTCAGGAAGATCGCCCCTGGAGTGAAAAGGAATATGACCGTCTGCATACGTTTACCCAAATTGTC
GGCGTGGTCGCCGAGCAAATTCAAAACCGGGTAAACAATAACGTCGATTATGATCTGTTGTGCCGCGAACGCGATAACTT
CCGTATTCTGGTTGCGATTACCAACGCCGTCCTGTCGCGTCTGGATATTGATGAACTGGTAAGCGAAGTCGCAAAAGAGA
TTCACCACTATTTCAATATCGACGCGATCAGCATCGTGTTGCGCAGCCATCGCAAAAATAAGCTGAATATCTACTCCACC
CACTACCTTGATGAGCATCATCCGGCGCACGAACAGAGCGAAGTCGACGAAGCCGGCACGCTCACGGAGCGCGTCTTTAA
AAGTAAAGAGATGCTGCTTATCAACCTCAACGAACGCGATCCGCTTGCCCCCTACGAACGCATGCTGTTCGATACCTGGG
GCAATCAGATCCAGACGCTTTGCCTGCTGCCGTTGATGTCCGGCAAGACGATGCTTGGCGTGCTCAAACTGGCCCAGTGC
GAAGAAAAAGTGTTTACCACCGCCAACCTCAAGCTGCTGCGTCAGATTGCCGAACGGGTAGCCATTGCCGTGGATAATGC
GCTGGCCTATCAGGAAATCCACCGACTGAAGGAACGCCTGGTCGACGAAAACCTGGCGCTCACGGAACAGCTTAATAACG
TCGACAGCGAGTTTGGCGAGATCATCGGACGCAGCGAAGCGATGTACAACGTGCTAAAGCAGGTTGAGATGGTGGCGCAG
AGCGACAGTACCGTGCTGATTCTGGGCGAAACGGGAACCGGTAAAGAGTTGATTGCCCGCGCCATTCACAACCTGAGCGG
ACGTAGCGGGCGCAGGATGGTAAAGATGAACTGCGCCGCGATGCCGGCGGGGCTGCTGGAAAGCGATCTGTTTGGTCATG
AGCGCGGCGCGTTTACCGGCGCCAGCGCGCAGCGGATTGGCCGTTTCGAGCTGGCGGATAAAAGCTCGCTGTTTCTGGAT
GAAGTGGGCGACATGCCGCTGGAGCTACAGCCTAAGCTGCTGCGCGTCCTTCAGGAGCAAGAGTTTGAACGTCTCGGCAG
CAATAAACTGATCCAGACCGACGTGCGGCTGATCGCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGATCGCGAAT
TCCGTAACGATCTCTACTATCGGCTGAATGTTTTTCCGATCCAGCTTCCTCCGTTGCGCGAACGTCCGGAAGATATTCCA
CTACTGGTCAAAGCGTTCACTTTCAAAATCGCCCGCCGAATGGGGCGTAATATCGACAGCATTCCCGCCGAAACGCTGCG
CACGCTTAGCAGCATGGAATGGCCGGGGAACGTTCGCGAGCTGGAGAATGTCGTCGAGCGCGCCGTGCTGCTGACGCGCG
GCAATGTGCTACAACTGTCGCTGCCGGATATTACCGCCGTAACGCCCGATACCTCGCCTGTTGCGACTGAAAGCGACAAA
GAAGGCGAAGATGAATATCAGCTTATTATACGCGTGCTGAAAGAGACCAATGGCGTAGTGGCTGGACCAAAAGGCGCGGC
GCAGCGTCTGGGGCTTAAACGAACCACCCTGCTGTCGCGTATGAAGCGATTGGGTATTGATAAAGACGCGTTAGCGTAA

Upstream 100 bases:

>100_bases
GCGAGTTTGCGGTCAGCCATCCGGCAATAACTTACGACTTATACCTATAATTCATTTACCGTTTTCGCACCGTTTTGCGG
TATTTTCCTGGAAAAGCAAC

Downstream 100 bases:

>100_bases
CGGGTTGCCGGATCTGATCCGGCCTGGAGATGATGCGCGCCGTAGGCCTGGCGCTTCCCCTTCACTTCTTGCGCTGATAT
TTTTCCGGCAATTCCGGAAC

Product: formate hydrogen-lyase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQYYATRENGRPVEYEDETVLA
HGPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHYCLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIV
GVVAEQIQNRVNNNVDYDLLCRERDNFRILVAITNAVLSRLDIDELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYST
HYLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTLCLLPLMSGKTMLGVLKLAQC
EEKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFRNDLYYRLNVFPIQLPPLRERPEDIP
LLVKAFTFKIARRMGRNIDSIPAETLRTLSSMEWPGNVRELENVVERAVLLTRGNVLQLSLPDITAVTPDTSPVATESDK
EGEDEYQLIIRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKDALA

Sequences:

>Translated_692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQYYATRENGRPVEYEDETVLA
HGPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHYCLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIV
GVVAEQIQNRVNNNVDYDLLCRERDNFRILVAITNAVLSRLDIDELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYST
HYLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTLCLLPLMSGKTMLGVLKLAQC
EEKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFRNDLYYRLNVFPIQLPPLRERPEDIP
LLVKAFTFKIARRMGRNIDSIPAETLRTLSSMEWPGNVRELENVVERAVLLTRGNVLQLSLPDITAVTPDTSPVATESDK
EGEDEYQLIIRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKDALA
>Mature_691_residues
SYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQYYATRENGRPVEYEDETVLAH
GPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHYCLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIVG
VVAEQIQNRVNNNVDYDLLCRERDNFRILVAITNAVLSRLDIDELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYSTH
YLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTLCLLPLMSGKTMLGVLKLAQCE
EKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQS
DSTVLILGETGTGKELIARAIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDE
VGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFRNDLYYRLNVFPIQLPPLRERPEDIPL
LVKAFTFKIARRMGRNIDSIPAETLRTLSSMEWPGNVRELENVVERAVLLTRGNVLQLSLPDITAVTPDTSPVATESDKE
GEDEYQLIIRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKDALA

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=691, Percent_Identity=90.4486251808973, Blast_Score=1289, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=572, Percent_Identity=50.6993006993007, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1788550, Length=320, Percent_Identity=46.5625, Blast_Score=275, Evalue=8e-75,
Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=48.2315112540193, Blast_Score=275, Evalue=1e-74,
Organism=Escherichia coli, GI1788905, Length=309, Percent_Identity=44.336569579288, Blast_Score=248, Evalue=1e-66,
Organism=Escherichia coli, GI87082152, Length=375, Percent_Identity=37.6, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1790299, Length=365, Percent_Identity=39.7260273972603, Blast_Score=235, Evalue=7e-63,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=41.875, Blast_Score=235, Evalue=8e-63,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=48.2758620689655, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1786524, Length=333, Percent_Identity=39.3393393393393, Blast_Score=198, Evalue=9e-52,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=35.5623100303951, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI87081858, Length=332, Percent_Identity=32.5301204819277, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=36.6141732283465, Blast_Score=142, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78298; Mature: 78166

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQ
CCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
YYATRENGRPVEYEDETVLAHGPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHY
EEEECCCCCCCCCCCCEEECCCHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCE
CLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIVGVVAEQIQNRVNNNVDYDLL
EEEEECCCCCEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
CRERDNFRILVAITNAVLSRLDIDELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEE
HYLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGKTMLGVLKLAQCEEKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQSDSTVLILGETGTGKELIAR
HCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFRNDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHEEECCHHHHHHHHHHHHHCCEEE
RLNVFPIQLPPLRERPEDIPLLVKAFTFKIARRMGRNIDSIPAETLRTLSSMEWPGNVRE
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVVERAVLLTRGNVLQLSLPDITAVTPDTSPVATESDKEGEDEYQLIIRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTTLLSRMKRLGIDKDALA
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SYTPMSDLGQQGLFDITRTLLQQPDLASLSEALSQLVKRSALADSAGIVLWQAQSQRAQ
CCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
YYATRENGRPVEYEDETVLAHGPVRRILSRPDALHCNFHEFTETWPQLAASGLYPEFGHY
EEEECCCCCCCCCCCCEEECCCHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCE
CLLPLAAEGRIFGGCEFIRQEDRPWSEKEYDRLHTFTQIVGVVAEQIQNRVNNNVDYDLL
EEEEECCCCCEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
CRERDNFRILVAITNAVLSRLDIDELVSEVAKEIHHYFNIDAISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEE
HYLDEHHPAHEQSEVDEAGTLTERVFKSKEMLLINLNERDPLAPYERMLFDTWGNQIQTL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGKTMLGVLKLAQCEEKVFTTANLKLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQSDSTVLILGETGTGKELIAR
HCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKKMVADREFRNDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHEEECCHHHHHHHHHHHHHCCEEE
RLNVFPIQLPPLRERPEDIPLLVKAFTFKIARRMGRNIDSIPAETLRTLSSMEWPGNVRE
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVVERAVLLTRGNVLQLSLPDITAVTPDTSPVATESDKEGEDEYQLIIRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTTLLSRMKRLGIDKDALA
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]