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The map label for this gene is yfiN [H]

Identifier: 16765987

GI number: 16765987

Start: 2813496

End: 2814716

Strand: Direct

Name: yfiN [H]

Synonym: STM2672

Alternate gene names: 16765987

Gene position: 2813496-2814716 (Clockwise)

Preceding gene: 16765986

Following gene: 16765993

Centisome position: 57.92

GC content: 50.45

Gene sequence:

>1221_bases
ATGAATAAGGAATTTTCTCTGTCCAGGCCAACATTTAAACGCACACTACGGCGGATTAGTATAATCAGCGTGCTGCTTAC
AATGACATTGATCTGGCTATTAATTTGCGTTGCGTCTGTCCTTACGCTCAAACAGTATGCGCAAAAGAATCTCGATTTGA
CCGCCGCCACAATGGCCCATAGCCTTGAAGCGGCACTGGTATTTTCCGATAACGCGGCCGCAGCGGAAACGCTCGCCACA
CTGGGACGCCAGGGACAATTTTCAGCGGCGGAGGTCCGCGATAAAAATGGCCGTACTATCGCCTCATGGCGCTATGATGC
GCGAGCCGCAGACGATAAGCTCATTGGCTTAATTAGCCACTGGCTTTTTCCATTGCCGGTATCACAACCCGTCTGGCACA
ACGGCAGGGCCATCGGCGAAGTACGGCTTGTCGCCCGCGACAGCCTTATTGGTCATTTTATCTGGCTATCGCTGGCAGTG
CTGACAGGATGTATTCTGCTGGCATCCGGCATTGCCCTGCTGCTCACGCGTTATTTACACAATGGCGTTGTGGATGCGCT
GCAAAATATTACTGAAGTTGTACACGACGTTCGCACTAACCGAAATTTTTCACGCCGGGTACCTGATGAGCGTATTGCGG
AATTTCACCTGTTTGCGCAGGATTTCAATAGCCTTCTGGATGAGATGGAAGAATGGCAGCTACGGCTTCAGGCTAAAAAT
GCCCAGTTACTACGTACCGCGTTGCACGATCCGCTGACGGGGCTTGCCAATCGCGCGGCATTTCGCAGCTGTATTAACGC
GCTGATGAAGGACAATTCCGCTCGTAGCAGTTCGGCATTGTTATTTCTGGATGGCGATAACTTTAAATATATTAATGATA
CCTGGGGACATGCGGCAGGCGACCGCGTACTTATAGAGGTTGCCAAAAGATTAGCGGAATTCGGTGGTAGCCGTTATCAG
ACTTACCGACTCGGCGGCGATGAATTTGCGATGGTGCTTTACGATGTACATTCGGAATATGAAGTACAACGTATTTGCGC
AGCGCTATCCCAGGCGTTTAATCGACCTTTTGAACTGCATAACGGCCAGCGAATAACGATGACCCTGAGTATTGGCTTTG
CGCTGACATGGGAACATGCCACTGCCGAAAAACTACAAGAACTGGCCGATCGAAATATGTATCAGGCTAAACACCGGCGT
GCGGAGCGCTCGCTAAACTAA

Upstream 100 bases:

>100_bases
AACAATGATGAAGTCAAATTTTCCGTCAACCTGGACTCCCTCTCGCATAGCGGCGTGAGAGTAAATCCAGAAGTATTAAT
GCTTGCACGGAATCAAAAGC

Downstream 100 bases:

>100_bases
GGAACGGGCCTGGCCGTTTCTGACTCAGCGTTGAGACCACTGACCCAGCCTAATCATGCAACCATCTATGGCGTGGAGCA
AACCCATAAAAAAGGGGCCA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDNAAAAETLAT
LGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAV
LTGCILLASGIALLLTRYLHNGVVDALQNITEVVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQ
TYRLGGDEFAMVLYDVHSEYEVQRICAALSQAFNRPFELHNGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRR
AERSLN

Sequences:

>Translated_406_residues
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDNAAAAETLAT
LGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAV
LTGCILLASGIALLLTRYLHNGVVDALQNITEVVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQ
TYRLGGDEFAMVLYDVHSEYEVQRICAALSQAFNRPFELHNGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRR
AERSLN
>Mature_406_residues
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAHSLEAALVFSDNAAAAETLAT
LGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISHWLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAV
LTGCILLASGIALLLTRYLHNGVVDALQNITEVVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAGDRVLIEVAKRLAEFGGSRYQ
TYRLGGDEFAMVLYDVHSEYEVQRICAALSQAFNRPFELHNGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRR
AERSLN

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=404, Percent_Identity=75.2475247524752, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI145693134, Length=152, Percent_Identity=35.5263157894737, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788381, Length=113, Percent_Identity=42.4778761061947, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787056, Length=314, Percent_Identity=27.0700636942675, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787262, Length=153, Percent_Identity=30.0653594771242, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=29.4871794871795, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=30.3571428571429, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=29.1139240506329, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=30.5882352941176, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787541, Length=150, Percent_Identity=30, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=30.2857142857143, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45767; Mature: 45767

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SLEAALVFSDNAAAAETLATLGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISH
HHHEEEEECCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHH
WLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAVLTGCILLASGIALLLTRYLH
HHCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGVVDALQNITEVVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
HHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCCCCC
DRVLIEVAKRLAEFGGSRYQTYRLGGDEFAMVLYDVHSEYEVQRICAALSQAFNRPFELH
CHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHEEHHCCHHHHHHHHHHHHHHHCCCEECC
NGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRRAERSLN
CCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKEFSLSRPTFKRTLRRISIISVLLTMTLIWLLICVASVLTLKQYAQKNLDLTAATMAH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SLEAALVFSDNAAAAETLATLGRQGQFSAAEVRDKNGRTIASWRYDARAADDKLIGLISH
HHHEEEEECCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHH
WLFPLPVSQPVWHNGRAIGEVRLVARDSLIGHFIWLSLAVLTGCILLASGIALLLTRYLH
HHCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGVVDALQNITEVVHDVRTNRNFSRRVPDERIAEFHLFAQDFNSLLDEMEEWQLRLQAKN
HHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLLRTALHDPLTGLANRAAFRSCINALMKDNSARSSSALLFLDGDNFKYINDTWGHAAG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCCCCC
DRVLIEVAKRLAEFGGSRYQTYRLGGDEFAMVLYDVHSEYEVQRICAALSQAFNRPFELH
CHHHHHHHHHHHHCCCCCEEEEEECCCCEEEHEEHHCCHHHHHHHHHHHHHHHCCCEECC
NGQRITMTLSIGFALTWEHATAEKLQELADRNMYQAKHRRAERSLN
CCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]