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The map label for this gene is yfiE [H]

Identifier: 16765964

GI number: 16765964

Start: 2784255

End: 2785154

Strand: Reverse

Name: yfiE [H]

Synonym: STM2644

Alternate gene names: 16765964

Gene position: 2785154-2784255 (Counterclockwise)

Preceding gene: 16765966

Following gene: 16765962

Centisome position: 57.34

GC content: 51.33

Gene sequence:

>900_bases
ATGGATCTGCGTCGGTTTATTACGCTGAAAACGGTAGTGGAAGAGGGCTCGTTTCTGCGGGCTTCGCAAAAGCTGTGCTG
CACTCAGTCGACGGTAACTTTTCATATTCAACAGCTTGAACGTGAGTTTTCCCTGCAACTGTTCGAGAAAATAGGGCGCC
GAATGTGCCTGACCAATGAGGGGAAAAAGCTGATGCCGCATATCCATGAACTGACGCGTGTGATGGAGTCGATTCGTGAA
GCCGCAAGACAGGATGCGGAACCCGGCGGAGAGCTGCGCGTGGCGACAGGTGAAACCTTACTGGCGTACAAAATGCCGCA
GGTTTTACAACGTTTTAAGCTGCGAGCGCCGAATGTCAGATTGTCGCTACAGTCGCTGAATTGCTATGTGATACGTGATG
CGTTGCTGAATGACGAGGTCGATCTGGGCGTTTTTTATCGCGTAGGTAATGATGACGCGCTCACGATGCAGCAATTGGGG
AAGCAGTCGCTGGCGCTGGTGGCCTCTCCACTGCTACAGGATGCTGATTTTACGCAGCCAGATCAGCATATTCCCTGTAG
CTTCATCATCAATGAACCGCAATGTGTCTTCCGCCAGCTGTTTGAAAGTACGCTACGGCAGAGGCGGATTACGCTGGAAA
ACACCATTGAACTGTGGAGTATTGAAAGCATTAAACAGTGTGTGGCTGCTAACCTGGGCATCAGCTTTTTACCACGTTTT
GCCGTTGAACGAGAACTTAGTACCGGTCAGTTGAAAGAGCTGCCGTTTGGCGCGCCGTCGCTATCGATCATGGCGCTGTG
CGCCCATCATGCCGGGAAAGCGGTCAGCCCGGCAATGCAGATATTTATGCAGTGTATGGAGGCGTGTTTTACCGTGGAAG
ATAAAAAAATGCCGGGTTAA

Upstream 100 bases:

>100_bases
GTCATCTCCTCTGTTTTAGCAGACAGTACCCGATATGAAATATTTAAAAAAATGAATGTTTTTTAATTCTGAATTCGAGA
TTTTCGATAGGTTGGTGGTT

Downstream 100 bases:

>100_bases
CGAACCCGGCATTATCATCACTCTTCGCTGGATTGCTCCTGCATTTTCGTACCGCTTGGCTTGCGGCGCTTACCGATATT
TTTTGTATCGCGGTGACGTT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNEGKKLMPHIHELTRVMESIRE
AARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVRLSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLG
KQSLALVASPLLQDADFTQPDQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
AVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG

Sequences:

>Translated_299_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNEGKKLMPHIHELTRVMESIRE
AARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVRLSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLG
KQSLALVASPLLQDADFTQPDQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
AVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG
>Mature_299_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNEGKKLMPHIHELTRVMESIRE
AARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVRLSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLG
KQSLALVASPLLQDADFTQPDQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
AVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=290, Percent_Identity=78.2758620689655, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=22.2996515679443, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=268, Percent_Identity=23.5074626865672, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=159, Percent_Identity=30.188679245283, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=24.4, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=157, Percent_Identity=29.9363057324841, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=22.9166666666667, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33866; Mature: 33866

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GKKLMPHIHELTRVMESIREAARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEE
LSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLGKQSLALVASPLLQDADFTQP
EEEECCCEEEEEHHHHCCCCCEEEEEEECCCCCHHHHHHCHHHHHHHHHHHHCCCCCCCC
DQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG
HHHHCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEREFSLQLFEKIGRRMCLTNE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GKKLMPHIHELTRVMESIREAARQDAEPGGELRVATGETLLAYKMPQVLQRFKLRAPNVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEE
LSLQSLNCYVIRDALLNDEVDLGVFYRVGNDDALTMQQLGKQSLALVASPLLQDADFTQP
EEEECCCEEEEEHHHHCCCCCEEEEEEECCCCCHHHHHHCHHHHHHHHHHHHCCCCCCCC
DQHIPCSFIINEPQCVFRQLFESTLRQRRITLENTIELWSIESIKQCVAANLGISFLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AVERELSTGQLKELPFGAPSLSIMALCAHHAGKAVSPAMQIFMQCMEACFTVEDKKMPG
HHHHCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]