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The map label for this gene is hmpA [H]

Identifier: 16765876

GI number: 16765876

Start: 2695259

End: 2696449

Strand: Direct

Name: hmpA [H]

Synonym: STM2556

Alternate gene names: 16765876

Gene position: 2695259-2696449 (Clockwise)

Preceding gene: 16765873

Following gene: 16765877

Centisome position: 55.49

GC content: 56.17

Gene sequence:

>1191_bases
ATGCTTGACGCACAAACCATCGCTACAGTAAAGGCCACCATTCCCCTGCTGGTTGAAACAGGACCGAAACTGACCGCCCA
CTTCTACGACCGTATGTTTACGCATAACCCGGAGCTCAAAGAAATCTTCAATATGAGCAACCAGCGTAACGGCGATCAGC
GTGAAGCCCTGTTTAATGCTATCGCGGCCTACGCCAGCAATATCGAAAATTTACCGGCGTTGCTGCCGGCGGTAGAAAAA
ATCGCGCAGAAGCACACCAGCTTCCAGATTAAGCCGGAGCAGTACAACATCGTCGGGACACATCTGCTGGCGACGCTGGA
CGAAATGTTCAACCCGGGCCAGGAAGTGCTGGACGCGTGGGGCAAAGCCTATGGCGTACTGGCTAACGTCTTTATTCATC
GGGAAGCCGAGATTTATCACGAGAACGCCAGCAAAGACGGCGGCTGGGAAGGCACGCGCCCCTTCCGCATCGTTGCGAAA
ACCCCGCGTAGCGCACTGATCACCAGCTTTGAGTTTGAACCAGTCGACGGCGGTACGGTGGCGGAATACCGTCCCGGGCA
GTATCTGGGCGTCTGGCTGAAGCCGGAAGGTTTTGCGCATCAGGAGATCCGCCAATATTCACTGACCCGTAAACCCGATG
GCAAAGGGTACCGTATTGCCGTGAAGCGTGAAGACGGCGGCCAGGTATCAAACTGGTTACACCATCACGCCAGCGTAGGC
GATGGGGTGCATCTGGCTGCGCCGGCAGGTGACTTCTTTATGAATGTCGCCGCTGATACCCCCGTTTCGCTGATTTCCGC
TGGCGTCGGCCAGACGCCGATGTTAGCGATGCTCGATACGCTGGCGAAAGAACAGCATACCGCGCAGGTGAACTGGTTCC
ACGCGGCGGAGAACGGCGACGTCCATGCGTTTGCCGACGAAGTGAGCGAGCTGGGCCGTACACTGCCGCGTTTCACTGCC
CATACCTGGTACCGCGAGCCGACTGAGTCCGATCGCGCCCAACGCCTCTTCGACAGCGAAGGGCTGATGGATTTAAGCAA
ACTGGAAGCCGCGATCAGCGATCCAGCAATGCAGTTCTATCTTTGCGGCCCGGTAGGCTTTATGCAGTTTGCCGCAAAAC
AACTGGTTTCACTTGGCGTGAATAACGAAAACATTCATTACGAATGCTTCGGCCCGCATAAAGTGCTGTAA

Upstream 100 bases:

>100_bases
TCCATAACGTAAAGCAGAGAAGATCCATTTACAATGCAAGGGTATTTTTATAAGATGCATTTGATATACATCATTAGATT
TTCACATAAAGGAAGCACGT

Downstream 100 bases:

>100_bases
TTGCAACAAATCCTCTGAAAAAACGGGCGTTATCGTTAAGGCGAACGCCCGTTTTCCTCCCCGTTCCCCTGCTTATTGCG
CTGCTTTTTATGCCTTTCTT

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIENLPALLPAVEK
IAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAWGKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAK
TPRSALITSFEFEPVDGGTVAEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHASVG
DGVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGDVHAFADEVSELGRTLPRFTA
HTWYREPTESDRAQRLFDSEGLMDLSKLEAAISDPAMQFYLCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL

Sequences:

>Translated_396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIENLPALLPAVEK
IAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAWGKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAK
TPRSALITSFEFEPVDGGTVAEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHASVG
DGVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGDVHAFADEVSELGRTLPRFTA
HTWYREPTESDRAQRLFDSEGLMDLSKLEAAISDPAMQFYLCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL
>Mature_396_residues
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNAIAAYASNIENLPALLPAVEK
IAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAWGKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAK
TPRSALITSFEFEPVDGGTVAEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHASVG
DGVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGDVHAFADEVSELGRTLPRFTA
HTWYREPTESDRAQRLFDSEGLMDLSKLEAAISDPAMQFYLCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=90.9090909090909, Blast_Score=752, Evalue=0.0,
Organism=Escherichia coli, GI1787658, Length=215, Percent_Identity=27.4418604651163, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787098, Length=202, Percent_Identity=28.2178217821782, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=410, Percent_Identity=35.1219512195122, Blast_Score=211, Evalue=2e-55,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 43992; Mature: 43992

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHH
IAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAW
HHHHHHCHHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHCCHHHHHHHH
GKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAKTPRSALITSFEFEPVDGGTV
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHEEEEEECCCCCCCC
AEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHASVG
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCC
DGVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGD
CCEEEECCCCHHHEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCC
VHAFADEVSELGRTLPRFTAHTWYREPTESDRAQRLFDSEGLMDLSKLEAAISDPAMQFY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEEE
LCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL
EECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNA
CCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHH
IAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGTHLLATLDEMFNPGQEVLDAW
HHHHHHCHHHHHHHHHHHHHHHHHHCCEEECCCCEEHHHHHHHHHHHHHHCCHHHHHHHH
GKAYGVLANVFIHREAEIYHENASKDGGWEGTRPFRIVAKTPRSALITSFEFEPVDGGTV
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHEEEEEECCCCCCCC
AEYRPGQYLGVWLKPEGFAHQEIRQYSLTRKPDGKGYRIAVKREDGGQVSNWLHHHASVG
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCC
DGVHLAAPAGDFFMNVAADTPVSLISAGVGQTPMLAMLDTLAKEQHTAQVNWFHAAENGD
CCEEEECCCCHHHEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCC
VHAFADEVSELGRTLPRFTAHTWYREPTESDRAQRLFDSEGLMDLSKLEAAISDPAMQFY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEEE
LCGPVGFMQFAAKQLVSLGVNNENIHYECFGPHKVL
EECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]