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The map label for this gene is sseA [H]

Identifier: 16765853

GI number: 16765853

Start: 2672906

End: 2673748

Strand: Direct

Name: sseA [H]

Synonym: STM2533

Alternate gene names: 16765853

Gene position: 2672906-2673748 (Clockwise)

Preceding gene: 16765832

Following gene: 16765854

Centisome position: 55.03

GC content: 59.31

Gene sequence:

>843_bases
ATGACCACCGCCTTTTTTGTCGCCGCCGACTGGCTTGCCGAACATATTGATGATCCGGAAATACAGATTCTTGACGCCCG
TATGGCGCCGCCAGGACAGGAACATCGTGATATGGCGGGCGAATATCGCGCCGGACATATTCCCGGTGCGCTGTTTTTTG
ATATTGAAGCGCTCTCCGATCGCGCATCGCCGTTGCCGCATATGATGCCGCGCCCGGAAGCGTTTGCGGTAGCCATGCGT
GAACTGGGTGTTCGTCAGGATAAACACCTGGTCATATACGATGAAGGCAATCTGTTCTCCGCTCCGCGGGCATGGTGGAT
GCTGCGCACGTTCGGTGCCGAGAAAGTGTCCATTCTGGCGGGCGGACTGGCGGGCTGGCAGCGCGACGAATGGCTATTGC
GCGAAGGCGAGGAAGCGCATGAAGGAGGCGAGTTTGAGGCGAAATTCGCGCCGCAAGCCGTGGTGCGTCTCACCGACGTA
TTGCTGGCAAGTCATGAAAAAACCGCGCAAATCGTTGACGCGCGTCCGGCGGCGCGTTTTAACGCTCAGGCTGATGAACC
GCGTCCGGGACTGAGGCGCGGACACATTCCTGGCGCGCTAAACGTCCCGTGGACGGAACTGGTGTATGAGGGCGAATTAA
AGACCACTGACGAACTGAATGAGGTTTTTTTCAGCCACGGCGTGAGTTTCGACAGACCGATTATCGCCAGCTGCGGTTCC
GGCGTGACGGCCGCGGTCGTGGTGCTGGCGCTCGCCACGCTGGATGTGCCTGACGTCACGCTTTACGACGGCGCGTGGAG
CGAATGGGGCGCGCGTACCGATTTACCGGTTGAACCCGCTTAA

Upstream 100 bases:

>100_bases
CAGAAATGTGGTTTCGCCAGCCAAAACAGCCTCTTCTACTTGTCCCTTTTCGGGAAACGTCCGACAATTTGACCATAATC
AGGTAACAATGGAGATGCCC

Downstream 100 bases:

>100_bases
TATCCGCCATGCCGGATGGCGCTCACGCTTATCCGGCCTACCGTTCAGGCTGATGTAAAACGATCTACAGACATTGTTTT
TATTCCGCTCTTTTCCGCTG

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTTAFFVAADWLAEHIDDPEIQILDARMAPPGQEHRDMAGEYRAGHIPGALFFDIEALSDRASPLPHMMPRPEAFAVAMR
ELGVRQDKHLVIYDEGNLFSAPRAWWMLRTFGAEKVSILAGGLAGWQRDEWLLREGEEAHEGGEFEAKFAPQAVVRLTDV
LLASHEKTAQIVDARPAARFNAQADEPRPGLRRGHIPGALNVPWTELVYEGELKTTDELNEVFFSHGVSFDRPIIASCGS
GVTAAVVVLALATLDVPDVTLYDGAWSEWGARTDLPVEPA

Sequences:

>Translated_280_residues
MTTAFFVAADWLAEHIDDPEIQILDARMAPPGQEHRDMAGEYRAGHIPGALFFDIEALSDRASPLPHMMPRPEAFAVAMR
ELGVRQDKHLVIYDEGNLFSAPRAWWMLRTFGAEKVSILAGGLAGWQRDEWLLREGEEAHEGGEFEAKFAPQAVVRLTDV
LLASHEKTAQIVDARPAARFNAQADEPRPGLRRGHIPGALNVPWTELVYEGELKTTDELNEVFFSHGVSFDRPIIASCGS
GVTAAVVVLALATLDVPDVTLYDGAWSEWGARTDLPVEPA
>Mature_279_residues
TTAFFVAADWLAEHIDDPEIQILDARMAPPGQEHRDMAGEYRAGHIPGALFFDIEALSDRASPLPHMMPRPEAFAVAMRE
LGVRQDKHLVIYDEGNLFSAPRAWWMLRTFGAEKVSILAGGLAGWQRDEWLLREGEEAHEGGEFEAKFAPQAVVRLTDVL
LASHEKTAQIVDARPAARFNAQADEPRPGLRRGHIPGALNVPWTELVYEGELKTTDELNEVFFSHGVSFDRPIIASCGSG
VTAAVVVLALATLDVPDVTLYDGAWSEWGARTDLPVEPA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=274, Percent_Identity=41.6058394160584, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI61835204, Length=274, Percent_Identity=41.6058394160584, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI194473681, Length=274, Percent_Identity=41.6058394160584, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI17402865, Length=275, Percent_Identity=38.9090909090909, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI87082121, Length=279, Percent_Identity=80.6451612903226, Blast_Score=467, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI115534702, Length=243, Percent_Identity=29.6296296296296, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17543836, Length=237, Percent_Identity=26.1603375527426, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17543838, Length=237, Percent_Identity=26.1603375527426, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71997283, Length=305, Percent_Identity=21.6393442622951, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17561888, Length=290, Percent_Identity=23.7931034482759, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17559150, Length=249, Percent_Identity=24.8995983935743, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324825, Length=247, Percent_Identity=34.412955465587, Blast_Score=136, Evalue=4e-33,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30832; Mature: 30701

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAFFVAADWLAEHIDDPEIQILDARMAPPGQEHRDMAGEYRAGHIPGALFFDIEALSD
CCEEEEEHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCEEEEEHHHHHC
RASPLPHMMPRPEAFAVAMRELGVRQDKHLVIYDEGNLFSAPRAWWMLRTFGAEKVSILA
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEECCCHHHHHHHHCCCCCEEEEE
GGLAGWQRDEWLLREGEEAHEGGEFEAKFAPQAVVRLTDVLLASHEKTAQIVDARPAARF
CCCCCCCCCHHHHHCCCCHHCCCCEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHC
NAQADEPRPGLRRGHIPGALNVPWTELVYEGELKTTDELNEVFFSHGVSFDRPIIASCGS
CCCCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCCC
GVTAAVVVLALATLDVPDVTLYDGAWSEWGARTDLPVEPA
CHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
TTAFFVAADWLAEHIDDPEIQILDARMAPPGQEHRDMAGEYRAGHIPGALFFDIEALSD
CEEEEEHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCEEEEEHHHHHC
RASPLPHMMPRPEAFAVAMRELGVRQDKHLVIYDEGNLFSAPRAWWMLRTFGAEKVSILA
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEECCCHHHHHHHHCCCCCEEEEE
GGLAGWQRDEWLLREGEEAHEGGEFEAKFAPQAVVRLTDVLLASHEKTAQIVDARPAARF
CCCCCCCCCHHHHHCCCCHHCCCCEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHC
NAQADEPRPGLRRGHIPGALNVPWTELVYEGELKTTDELNEVFFSHGVSFDRPIIASCGS
CCCCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCCC
GVTAAVVVLALATLDVPDVTLYDGAWSEWGARTDLPVEPA
CHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]