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The map label for this gene is apbE

Identifier: 16765594

GI number: 16765594

Start: 2364276

End: 2365328

Strand: Reverse

Name: apbE

Synonym: STM2266

Alternate gene names: 16765594

Gene position: 2365328-2364276 (Counterclockwise)

Preceding gene: 16765595

Following gene: 16765593

Centisome position: 48.7

GC content: 56.41

Gene sequence:

>1053_bases
ATGAAAATGACTTTTTGCCGGGCCGTGTGTCTGGCGGCGGCTTTTCTACTTATGGGCTGCGATGAGGCTCCCGAAACGAC
AACAGCGTCACCTGCCGCTCAGGTGCTGGAAGGTAAAACGATGGGGACTCTCTGGCGGGTGAGCGTGGTTGGTATCGATG
CGAAACGCGCCGCAGAGTTACAGACTAAAATCCAGACTCAGCTTGATGCTGATGATTGGTTGCTTTCTACCTATAAAAAT
GACTCCGCGTTGATGCGTTTTAACCATTCACGCAGTCTTGCGCCCTGGCCGGTCAGCGAAGCCATGGCGGATATCGTGAC
CTCGGCGCTGCGTATTGGCGCGAAGACGGATGGCGCGATGGATATCACCGTGGGCCCGCTGGTCAATCTGTGGGGGTTTG
GGCCGGATCGGCAGCCGCTGCATATCCCAACACCAGCACAAATCGATGCGGCAAAAGCGAAAACAGGCCTGCAACATTTG
CAGGTTATCGACAGGGCTGGACATCAGTTTTTGCAAAAAGATCTGCCGGATCTTTATGTTGATCTCTCCACGGTCGGGGA
GGGCTATGCGGCGGATCATCTGGCGCGTCTGATGGAGCAGGAGGGCATTGCGCGTTATCTGGTCTCGGTGGGCGGCGCAT
TAAGCAGTCGCGGGATGAATGCGCAGGGGCAGCCGTGGCGCGTCGCGATTCAGAAGCCGACCGACCGGGAAAACGCAGTG
CAGGCGATTGTGGATATCAACGGGCATGGCATCAGCACCTCCGGCAGCTACCGTAACTATTACGAGCTGGATGGCAAGCG
TGTCTCGCACGTTATCGATCCGCAAACGGGGCGCCCCATTGAACACAATCTGGTATCGGTTACGGTCATCGCGCCAACGG
CGTTGGAAGCGGACGGCTGGGACACCGGCCTGATGGTGCTTGGTACGCAAAAGGCGCAAGAGGTCGTGCGGAGGGAAGGG
CTGGCGGTATTTATGATCATGAAAGAAGGTGAAGGCTTTAAAACCTGGATGTCGCCGCAGTTCAAAACGTTCCTGGTGAG
CGATAAGAATTAA

Upstream 100 bases:

>100_bases
TGCTAAAGGGCCTGCGGGCCCTTTTTTCATGCCTTATTCCGGCGTACAAATACGACGTTTTGATATACGCTTGCGTCCAT
TCGAATGAGGATAAACAGCG

Downstream 100 bases:

>100_bases
AACGCAAGATTACGGGTTTTTGTGGGCGGATTTACGGACACACTGCATGTAAGCAAGCACTATGCTTAATGAAGGAGTCT
ATGATGAAAAAAGCGTTACT

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTLWRVSVVGIDAKRAAELQTKIQTQLDADDWLLSTYKN
DSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAMDITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHL
QVIDRAGHQFLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGQPWRVAIQKPTDRENAV
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGWDTGLMVLGTQKAQEVVRREG
LAVFMIMKEGEGFKTWMSPQFKTFLVSDKN

Sequences:

>Translated_350_residues
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTLWRVSVVGIDAKRAAELQTKIQTQLDADDWLLSTYKN
DSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAMDITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHL
QVIDRAGHQFLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGQPWRVAIQKPTDRENAV
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGWDTGLMVLGTQKAQEVVRREG
LAVFMIMKEGEGFKTWMSPQFKTFLVSDKN
>Mature_350_residues
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTLWRVSVVGIDAKRAAELQTKIQTQLDADDWLLSTYKN
DSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAMDITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHL
QVIDRAGHQFLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGQPWRVAIQKPTDRENAV
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGWDTGLMVLGTQKAQEVVRREG
LAVFMIMKEGEGFKTWMSPQFKTFLVSDKN

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family

Homologues:

Organism=Escherichia coli, GI1788543, Length=351, Percent_Identity=80.0569800569801, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): APBE_SALTY (P41780)

Other databases:

- EMBL:   AF035376
- EMBL:   U90324
- EMBL:   AE006468
- EMBL:   D90221
- RefSeq:   NP_461209.1
- ProteinModelPortal:   P41780
- SMR:   P41780
- PRIDE:   P41780
- GeneID:   1253788
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2266
- HOGENOM:   HBG292712
- OMA:   MAEIVTT
- ProtClustDB:   PRK10461
- BioCyc:   STYP99287:STM2266-MONOMER
- InterPro:   IPR003374

Pfam domain/function: PF02424 ApbE

EC number: NA

Molecular weight: Translated: 38149; Mature: 38149

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTLWRVSVVGIDAKRAAEL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEECCHHHHHHH
QTKIQTQLDADDWLLSTYKNDSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAM
HHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
DITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHLQVIDRAGHQFLQKDLPDLYV
EEEHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEE
DLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGQPWRVAIQKPTDRENAV
EHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEECCCCHHHHE
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGW
EEEEEECCCCCCCCCCCCCEEECCCCEEHEEECCCCCCCCCCCEEEEEEEECCEECCCCC
DTGLMVLGTQKAQEVVRREGLAVFMIMKEGEGFKTWMSPQFKTFLVSDKN
CCCEEEEECHHHHHHHHHCCCEEEEEEECCCCCCHHCCCCEEEEEEECCC
>Mature Secondary Structure
MKMTFCRAVCLAAAFLLMGCDEAPETTTASPAAQVLEGKTMGTLWRVSVVGIDAKRAAEL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEECCHHHHHHH
QTKIQTQLDADDWLLSTYKNDSALMRFNHSRSLAPWPVSEAMADIVTSALRIGAKTDGAM
HHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
DITVGPLVNLWGFGPDRQPLHIPTPAQIDAAKAKTGLQHLQVIDRAGHQFLQKDLPDLYV
EEEHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEE
DLSTVGEGYAADHLARLMEQEGIARYLVSVGGALSSRGMNAQGQPWRVAIQKPTDRENAV
EHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEECCCCHHHHE
QAIVDINGHGISTSGSYRNYYELDGKRVSHVIDPQTGRPIEHNLVSVTVIAPTALEADGW
EEEEEECCCCCCCCCCCCCEEECCCCEEHEEECCCCCCCCCCCEEEEEEEECCEECCCCC
DTGLMVLGTQKAQEVVRREGLAVFMIMKEGEGFKTWMSPQFKTFLVSDKN
CCCEEEEECHHHHHHHHHCCCEEEEEEECCCCCCHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9473043; 11677609; 1904855