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The map label for this gene is wcaM

Identifier: 16765429

GI number: 16765429

Start: 2180028

End: 2181431

Strand: Reverse

Name: wcaM

Synonym: STM2099

Alternate gene names: 16765429

Gene position: 2181431-2180028 (Counterclockwise)

Preceding gene: 16765430

Following gene: 16765428

Centisome position: 44.91

GC content: 51.21

Gene sequence:

>1404_bases
ATGCCCGCGACTAAATTCTCCCGACGTACCCTCCTGACGGCAGGTTCTGCGCTTGCTGTTCTTCCTTTTCTGCGCGCCTT
GCCGGTACAGGCGCGTGAACCTCGCGAGACCGTCGATATTAAGGATTATCCGGCGGATGACGGTATCGCCTCGTTCAAAC
AGGCCTTCGCCGACGGACAGACCGTGGTCGTACCGCCAGGATGGGTGTGTGAAAATATCAATGCGGCGATAACGATTCCG
GCGGGAAAAACGCTGCGGGTACAGGGCGCGGTGCGTGGGAATGGCCGGGGACGGTTTATTTTGCAGGACGGGTGTCAGGT
GGTGGGGGAGCAGGGCGGCAGTCTGCACAATGTGACGCTGGATGTTCGCGGGTCGGACTGTGTGATTAAAGGCGTGGCGA
TGAGCGGCTTTGGCCCCGTCGCGCAAATTTTCATCGGTGGTAAGGAACCGCAGGTGATGCGTAATCTCATTATCGATGAC
ATCACCGTTACCCACGCCAACTACGCCATTCTCCGCCAGGGATTTCATAACCAAATGGATGGCGCGCGGATTACGCATAG
CCGCTTTAGCGATTTACAGGGGGACGCCATTGAGTGGAATGTCGCGATTCACGACCGCGACATCCTGATTTCCGATCATG
TCATCGAACGCATTAATTGTACCAATGGCAAAATCAACTGGGGGATCGGCATCGGGCTGGCGGGTAGCACCTATGACAAC
AGTTATCCTGAAGACCAGGCAGTAAAAAACTTTGTGGTGGCCAATATTACCGGATCTGATTGCCGACAGCTTGTGCACGT
AGAAAATGGCAAACATTTCGTCATTCGCAATGTCAAAGCCAAAAACATCACGCCCGGTTTCAGTAAAAATGCGGGTATTG
ATAACGCAACGATCGCAATTTATGGCTGTGATAATTTCGTCATTGATAATATTGATATGACGAATAGTGCCGGGATGCTC
ATCGGCTATGGCGTCGTTAAAGGAAAATACCTGTCAATTCCGCAAAACTTTAAATTAAACGCTATTCGGTTGGATAATCG
CCAGGTTGCTTATAAATTACGCGGCATTCAAATTTCCTCCGGCAACACCCCCTCTTTTGTCGCCATCACCAATGTACGGA
TGACGCGTGCTACGCTGGAACTGCATAATCAACCGCAGCACCTCTTTCTGCGCAATATCAACGTGATGCAAACTTCAGCG
ATTGGCCCGGCGTTAAAAATGCATTTCGATTTGCGTAAAGATGTACGTGGTCAATTTATGGCCCGCCAGGACACGCTGCT
TTCCCTCGCTAATGTTCATGCCATCAATGAAAACGGGCAGAGTTCCGTGGATATCGACAGGATTAATCACCAAACCGTGA
ATGTCGAAGCAGTGAATTTTTCGCTGCCGAAGCGGGGAGGGTAA

Upstream 100 bases:

>100_bases
GTGATCACGCGCGCCCGTGAAAAAGTGGCGCAAGATTTTAATCAGCAGGCGATTAATCGCCAGTTAGCCAGCCTGCTACA
AACGATATAAACGAGGTGGT

Downstream 100 bases:

>100_bases
GTACCGCTATTTTTACGAAAATTCCTGGGAAAAAGTTGTTCATACTTAATGTTATGGTGCCGACTAAGACGTAATGTAGA
GCGTGCCATCATTATCCCTG

Product: colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MPATKFSRRTLLTAGSALAVLPFLRALPVQAREPRETVDIKDYPADDGIASFKQAFADGQTVVVPPGWVCENINAAITIP
AGKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTLDVRGSDCVIKGVAMSGFGPVAQIFIGGKEPQVMRNLIIDD
ITVTHANYAILRQGFHNQMDGARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDN
SYPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPGFSKNAGIDNATIAIYGCDNFVIDNIDMTNSAGML
IGYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISSGNTPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSA
IGPALKMHFDLRKDVRGQFMARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG

Sequences:

>Translated_467_residues
MPATKFSRRTLLTAGSALAVLPFLRALPVQAREPRETVDIKDYPADDGIASFKQAFADGQTVVVPPGWVCENINAAITIP
AGKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTLDVRGSDCVIKGVAMSGFGPVAQIFIGGKEPQVMRNLIIDD
ITVTHANYAILRQGFHNQMDGARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDN
SYPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPGFSKNAGIDNATIAIYGCDNFVIDNIDMTNSAGML
IGYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISSGNTPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSA
IGPALKMHFDLRKDVRGQFMARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG
>Mature_466_residues
PATKFSRRTLLTAGSALAVLPFLRALPVQAREPRETVDIKDYPADDGIASFKQAFADGQTVVVPPGWVCENINAAITIPA
GKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTLDVRGSDCVIKGVAMSGFGPVAQIFIGGKEPQVMRNLIIDDI
TVTHANYAILRQGFHNQMDGARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDNS
YPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPGFSKNAGIDNATIAIYGCDNFVIDNIDMTNSAGMLI
GYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISSGNTPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSAI
GPALKMHFDLRKDVRGQFMARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788356, Length=466, Percent_Identity=77.0386266094421, Blast_Score=752, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WCAM_SALTY (P26389)

Other databases:

- EMBL:   X56793
- EMBL:   AE006468
- PIR:   S15297
- RefSeq:   NP_461044.1
- ProteinModelPortal:   P26389
- GeneID:   1253620
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2099
- NMPDR:   fig|99287.1.peg.2024
- HOGENOM:   HBG417270
- OMA:   HAVNERG
- ProtClustDB:   PRK10123
- BioCyc:   STYP99287:STM2099-MONOMER
- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.160.20.10

Pfam domain/function: SSF51126 Pectin_lyas_like

EC number: NA

Molecular weight: Translated: 50959; Mature: 50828

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATKFSRRTLLTAGSALAVLPFLRALPVQAREPRETVDIKDYPADDGIASFKQAFADGQ
CCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
TVVVPPGWVCENINAAITIPAGKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTL
EEEECCCCEECCCCEEEEECCCCEEEEEEEEECCCCEEEEECCCHHHHHCCCCCEEEEEE
DVRGSDCVIKGVAMSGFGPVAQIFIGGKEPQVMRNLIIDDITVTHANYAILRQGFHNQMD
EECCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCEEEECCCHHHHHHCCCCCCC
GARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDN
CCEEEHHHHCCCCCCEEEEEEEEECCEEEEECHHHHEEECCCCEEEEEEEEEECCCCCCC
SYPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPGFSKNAGIDNATIAI
CCCCHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEE
YGCDNFVIDNIDMTNSAGMLIGYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISS
EECCCEEEECCCCCCCCCEEEEEEEECCEEEECCCCCEEEEEEECCCEEEEEEEEEEEEC
GNTPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSAIGPALKMHFDLRKDVRGQFM
CCCCCEEEEEEEEEEEEEEEECCCCCEEEEECCCEEEECCCCCEEEEEEHHHHHHHHHHH
ARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG
HHHHHHHHHHHEEEECCCCCCCEEEECCCCEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PATKFSRRTLLTAGSALAVLPFLRALPVQAREPRETVDIKDYPADDGIASFKQAFADGQ
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
TVVVPPGWVCENINAAITIPAGKTLRVQGAVRGNGRGRFILQDGCQVVGEQGGSLHNVTL
EEEECCCCEECCCCEEEEECCCCEEEEEEEEECCCCEEEEECCCHHHHHCCCCCEEEEEE
DVRGSDCVIKGVAMSGFGPVAQIFIGGKEPQVMRNLIIDDITVTHANYAILRQGFHNQMD
EECCCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCEEEECCCHHHHHHCCCCCCC
GARITHSRFSDLQGDAIEWNVAIHDRDILISDHVIERINCTNGKINWGIGIGLAGSTYDN
CCEEEHHHHCCCCCCEEEEEEEEECCEEEEECHHHHEEECCCCEEEEEEEEEECCCCCCC
SYPEDQAVKNFVVANITGSDCRQLVHVENGKHFVIRNVKAKNITPGFSKNAGIDNATIAI
CCCCHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEE
YGCDNFVIDNIDMTNSAGMLIGYGVVKGKYLSIPQNFKLNAIRLDNRQVAYKLRGIQISS
EECCCEEEECCCCCCCCCEEEEEEEECCEEEECCCCCEEEEEEECCCEEEEEEEEEEEEC
GNTPSFVAITNVRMTRATLELHNQPQHLFLRNINVMQTSAIGPALKMHFDLRKDVRGQFM
CCCCCEEEEEEEEEEEEEEEECCCCCEEEEECCCEEEECCCCCEEEEEEHHHHHHHHHHH
ARQDTLLSLANVHAINENGQSSVDIDRINHQTVNVEAVNFSLPKRGG
HHHHHHHHHHHEEEECCCCCCCEEEECCCCEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609