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The map label for this gene is dsbE [H]

Identifier: 167626005

GI number: 167626005

Start: 5002152

End: 5002706

Strand: Reverse

Name: dsbE [H]

Synonym: Shal_4100

Alternate gene names: 167626005

Gene position: 5002706-5002152 (Counterclockwise)

Preceding gene: 167626006

Following gene: 167626004

Centisome position: 95.71

GC content: 44.14

Gene sequence:

>555_bases
ATGAAAAAGTTAGTATTATTTATTCCACTCGTCGTAGTTTTAGGTATGGGAGTGTTCCTCTATAAGGGACTCTTCCTTAA
CCCGCAACAACTCGACTCAGCTCTAGAAGGAAAGCCGATCCCGGCTTTCCAGTTAGAGGTTTTGGAGAAGTCAGGGGAGA
CCGTGACTAATGAAACGCTAAAAGGCAAGGTAGGCTTACTTAATGTGTGGGCAACTTGGTGTCCATCATGTAAGTATGAG
CATCCTTTCCTAATGAGCTTAGCGCGCAAAAATATTCTACCTATATATGGTATTAACTATCGCGATGAACGTGCATTAGC
TATTCGTGAGTTAAGCCGTGAAGGTAACCCATACGCAATTAATATCTATGACCATGACGGTCGCCTAGGTTTAGACCTTG
GTGTATACGGTGCGCCAGAGAGTTTCCTTGTCGATCATAAAGGTGTTATTCGTTACCGTTATGCGGGGCCAATTGACCGT
AATGTATGGAAAGAAACACTTTACCCTATGGTTCAACAGCTACAAGCTGAAGCGGCGAAGGATGGTGCATCGTGA

Upstream 100 bases:

>100_bases
GCATCTGATAAGCGCTACCGCGTAAGAGCTAAGAGCGCAGAAAAGACTGCAGCTAAAGAAGCTGACAAGTTAGCAACGGC
TTAATTGGAACAGAGTAAAT

Downstream 100 bases:

>100_bases
TTAAATCATTAATGAAGGGCTTTTCGGCTCTAGTGCTTCTACTAAGCATGGCAACGATCGTTAATGCTACACCAGTCGAC
ACTTATGAGTTTAAGTCTGT

Product: periplasmic protein thiol--disulfide oxidoreductase DsbE

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CcmG [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MKKLVLFIPLVVVLGMGVFLYKGLFLNPQQLDSALEGKPIPAFQLEVLEKSGETVTNETLKGKVGLLNVWATWCPSCKYE
HPFLMSLARKNILPIYGINYRDERALAIRELSREGNPYAINIYDHDGRLGLDLGVYGAPESFLVDHKGVIRYRYAGPIDR
NVWKETLYPMVQQLQAEAAKDGAS

Sequences:

>Translated_184_residues
MKKLVLFIPLVVVLGMGVFLYKGLFLNPQQLDSALEGKPIPAFQLEVLEKSGETVTNETLKGKVGLLNVWATWCPSCKYE
HPFLMSLARKNILPIYGINYRDERALAIRELSREGNPYAINIYDHDGRLGLDLGVYGAPESFLVDHKGVIRYRYAGPIDR
NVWKETLYPMVQQLQAEAAKDGAS
>Mature_184_residues
MKKLVLFIPLVVVLGMGVFLYKGLFLNPQQLDSALEGKPIPAFQLEVLEKSGETVTNETLKGKVGLLNVWATWCPSCKYE
HPFLMSLARKNILPIYGINYRDERALAIRELSREGNPYAINIYDHDGRLGLDLGVYGAPESFLVDHKGVIRYRYAGPIDR
NVWKETLYPMVQQLQAEAAKDGAS

Specific function: Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=186, Percent_Identity=47.8494623655914, Blast_Score=174, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 20698; Mature: 20698

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLFIPLVVVLGMGVFLYKGLFLNPQQLDSALEGKPIPAFQLEVLEKSGETVTNETL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEECCHHH
KGKVGLLNVWATWCPSCKYEHPFLMSLARKNILPIYGINYRDERALAIRELSREGNPYAI
CCCCHHEEHHHHHCCCCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEE
NIYDHDGRLGLDLGVYGAPESFLVDHKGVIRYRYAGPIDRNVWKETLYPMVQQLQAEAAK
EEEECCCEEEEEECCCCCCHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
DGAS
CCCC
>Mature Secondary Structure
MKKLVLFIPLVVVLGMGVFLYKGLFLNPQQLDSALEGKPIPAFQLEVLEKSGETVTNETL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEECCHHH
KGKVGLLNVWATWCPSCKYEHPFLMSLARKNILPIYGINYRDERALAIRELSREGNPYAI
CCCCHHEEHHHHHCCCCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEE
NIYDHDGRLGLDLGVYGAPESFLVDHKGVIRYRYAGPIDRNVWKETLYPMVQQLQAEAAK
EEEECCCEEEEEECCCCCCHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
DGAS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]