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The map label for this gene is frmA [H]

Identifier: 167625938

GI number: 167625938

Start: 4913813

End: 4914943

Strand: Reverse

Name: frmA [H]

Synonym: Shal_4033

Alternate gene names: 167625938

Gene position: 4914943-4913813 (Counterclockwise)

Preceding gene: 167625939

Following gene: 167625937

Centisome position: 94.03

GC content: 46.33

Gene sequence:

>1131_bases
ATGACAGCACAAACAATTAAATCAAAAGCCGCAGTTGCTTGGGCCGTTGGCGAGCCACTATCTATGGAAATCGTAGATGT
TATGCCACCTCAAAAAGGTGAAGTGCGCGTTAAGATGATCGCCACTGGTGTATGCCACACCGATGCATTCACTCTTTCAG
GTGACGATCCAGAAGGGATCTTCCCTTGTATTCTTGGCCATGAAGGTGGCGGTATCGTTGAGTCTATTGGTGAAGGCGTA
ACTAGCGTTCAAGTCGGTGACCACGTGATCCCACTTTACACTCCTGAGTGTGGCGAATGTAAATTCTGTAAGTCTGGCAA
GACTAACCTTTGTCAGAAGATCCGTGAAACTCAAGGTAAAGGTTTAATGCCTGATGGTACGAGTCGTTTCTCTAAAGATG
GTCAGATAATTTACCACTACATGGGCACTTCTACTTTCTCTGAGTACACGGTATTGCCTGAGATTTCGCTCGCTAAAGTT
AACCCAGAAGCGCCACTAGAAGAAGTCTGTTTACTCGGTTGTGGTGTCACAACGGGTATGGGTGCGGTAATGAACACGGC
TAAAGTTGAAGAGGGCGCAACTGTTGCTATCTTCGGTATGGGCGGTATCGGTCTATCGGCTGTTATTGGCGCGGCAATGG
CTAAAGCCTCACGCATCATTGCTATTGACATCAATGAAAGTAAGTTTGACCTTGCTCGTAAGTTGGGTGCAACTGATTGC
ATTAACCCTAAAGATTACGACAAGCCAATCCAAGATGTGATTGTTGAGCTAACAGATGGCGGTGTTGATTACTCATTCGA
GTGTATCGGTAACGTACACGTGATGCGTAGCGCACTTGAGTGCTGTCATAAAGGTTGGGGTGAGTCAGTCGTTATTGGTG
TTGCGGGTGCAGGCCAAGAGATCTCAACTCGTCCATTCCAATTAGTGACTGGTCGTGTGTGGAAAGGTAGTGCGTTTGGT
GGTGTTAAAGGTCGCTCTGAGCTTCCAGATTATGTTGAGCGTTACCTTGCAGGCGAGTTCAAGTTGAGTGACTTTATTAC
TCACACTATGAGCCTTGAGCAAGTAAACGAAGCATTCGACCTAATGCACGAAGGTAAGAGTATTCGTACTGTTATTCACT
TCGACAAGTAA

Upstream 100 bases:

>100_bases
TTGATGGGATTATCAATATTGAAGAGGTAGTTATAATCTTTGCCATACCCACGGCGGTGAAGCACCGCAATTTAAAGATT
ATTAAAAGTAGGAATACAAG

Downstream 100 bases:

>100_bases
ATAGTTAGTCAAGATAACAAGCGCTCTCGAGCGCTTGTTGTTTTTAAAGGCCAGATTTTTAAAGATTGAACATCTATTGG
TTTAGCTTTTAAAGATTGGG

Product: S-(hydroxymethyl)glutathione dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTAQTIKSKAAVAWAVGEPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGIFPCILGHEGGGIVESIGEGV
TSVQVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGKGLMPDGTSRFSKDGQIIYHYMGTSTFSEYTVLPEISLAKV
NPEAPLEEVCLLGCGVTTGMGAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDC
INPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQEISTRPFQLVTGRVWKGSAFG
GVKGRSELPDYVERYLAGEFKLSDFITHTMSLEQVNEAFDLMHEGKSIRTVIHFDK

Sequences:

>Translated_376_residues
MTAQTIKSKAAVAWAVGEPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGIFPCILGHEGGGIVESIGEGV
TSVQVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGKGLMPDGTSRFSKDGQIIYHYMGTSTFSEYTVLPEISLAKV
NPEAPLEEVCLLGCGVTTGMGAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDC
INPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQEISTRPFQLVTGRVWKGSAFG
GVKGRSELPDYVERYLAGEFKLSDFITHTMSLEQVNEAFDLMHEGKSIRTVIHFDK
>Mature_375_residues
TAQTIKSKAAVAWAVGEPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGIFPCILGHEGGGIVESIGEGVT
SVQVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGKGLMPDGTSRFSKDGQIIYHYMGTSTFSEYTVLPEISLAKVN
PEAPLEEVCLLGCGVTTGMGAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDCI
NPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQEISTRPFQLVTGRVWKGSAFGG
VKGRSELPDYVERYLAGEFKLSDFITHTMSLEQVNEAFDLMHEGKSIRTVIHFDK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=372, Percent_Identity=65.3225806451613, Blast_Score=517, Evalue=1e-147,
Organism=Homo sapiens, GI34577061, Length=371, Percent_Identity=53.099730458221, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI4501929, Length=371, Percent_Identity=51.2129380053908, Blast_Score=407, Evalue=1e-114,
Organism=Homo sapiens, GI4501933, Length=371, Percent_Identity=50.9433962264151, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=51.7333333333333, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI156523966, Length=371, Percent_Identity=49.0566037735849, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI4501939, Length=365, Percent_Identity=49.3150684931507, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI262073058, Length=372, Percent_Identity=48.3870967741936, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI71743840, Length=371, Percent_Identity=48.2479784366577, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=71.5447154471545, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI1790045, Length=362, Percent_Identity=26.5193370165746, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082125, Length=361, Percent_Identity=26.0387811634349, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787863, Length=356, Percent_Identity=25.2808988764045, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081918, Length=349, Percent_Identity=29.2263610315186, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788073, Length=351, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788407, Length=231, Percent_Identity=27.7056277056277, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786825, Length=265, Percent_Identity=26.0377358490566, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1786518, Length=368, Percent_Identity=24.1847826086957, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI226510992, Length=350, Percent_Identity=22.5714285714286, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=377, Percent_Identity=59.946949602122, Blast_Score=491, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71997431, Length=379, Percent_Identity=59.6306068601583, Blast_Score=486, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17565904, Length=376, Percent_Identity=55.3191489361702, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17562584, Length=363, Percent_Identity=30.5785123966942, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17562876, Length=371, Percent_Identity=25.8760107816712, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71988145, Length=371, Percent_Identity=27.2237196765499, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=380, Percent_Identity=27.6315789473684, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562878, Length=371, Percent_Identity=25.6064690026954, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=377, Percent_Identity=57.8249336870027, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6319621, Length=386, Percent_Identity=30.0518134715026, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=378, Percent_Identity=28.042328042328, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=366, Percent_Identity=28.1420765027322, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=323, Percent_Identity=26.9349845201238, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322619, Length=362, Percent_Identity=26.7955801104972, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319955, Length=362, Percent_Identity=26.7955801104972, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=335, Percent_Identity=27.4626865671642, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319258, Length=218, Percent_Identity=26.605504587156, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17737895, Length=373, Percent_Identity=64.6112600536193, Blast_Score=510, Evalue=1e-145,
Organism=Drosophila melanogaster, GI17137530, Length=347, Percent_Identity=27.3775216138329, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17737897, Length=255, Percent_Identity=27.4509803921569, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 40199; Mature: 40067

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTIKSKAAVAWAVGEPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGI
CCCHHHHCCCEEEEECCCCCCEEEEECCCCCCCCEEEEEEEECEEECCEEEECCCCCCCC
FPCILGHEGGGIVESIGEGVTSVQVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGK
EEEEEECCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
GLMPDGTSRFSKDGQIIYHYMGTSTFSEYTVLPEISLAKVNPEAPLEEVCLLGCGVTTGM
CCCCCCCHHCCCCCCEEEEEECCCCCCCEEECCCCEEEECCCCCCHHHHHHHHCCHHHHH
GAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDC
HHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCCC
INPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQE
CCCCCCCCCHHHHHEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
ISTRPFQLVTGRVWKGSAFGGVKGRSELPDYVERYLAGEFKLSDFITHTMSLEQVNEAFD
CCCCCEEEEECEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LMHEGKSIRTVIHFDK
HHHCCCCEEEEEEECC
>Mature Secondary Structure 
TAQTIKSKAAVAWAVGEPLSMEIVDVMPPQKGEVRVKMIATGVCHTDAFTLSGDDPEGI
CCHHHHCCCEEEEECCCCCCEEEEECCCCCCCCEEEEEEEECEEECCEEEECCCCCCCC
FPCILGHEGGGIVESIGEGVTSVQVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGK
EEEEEECCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
GLMPDGTSRFSKDGQIIYHYMGTSTFSEYTVLPEISLAKVNPEAPLEEVCLLGCGVTTGM
CCCCCCCHHCCCCCCEEEEEECCCCCCCEEECCCCEEEECCCCCCHHHHHHHHCCHHHHH
GAVMNTAKVEEGATVAIFGMGGIGLSAVIGAAMAKASRIIAIDINESKFDLARKLGATDC
HHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCCCCC
INPKDYDKPIQDVIVELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQE
CCCCCCCCCHHHHHEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
ISTRPFQLVTGRVWKGSAFGGVKGRSELPDYVERYLAGEFKLSDFITHTMSLEQVNEAFD
CCCCCEEEEECEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LMHEGKSIRTVIHFDK
HHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]