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The map label for this gene is dapF [H]

Identifier: 167625817

GI number: 167625817

Start: 4747990

End: 4748850

Strand: Reverse

Name: dapF [H]

Synonym: Shal_3912

Alternate gene names: 167625817

Gene position: 4748850-4747990 (Counterclockwise)

Preceding gene: 167625818

Following gene: 167625816

Centisome position: 90.85

GC content: 47.15

Gene sequence:

>861_bases
ATGCGCTACAGATACTTTTTAAGGATGTCCACCTTGATCCAATTCACTAAGATGCATGGGTTAGGCAATGATTTTATGGT
CGTCGATGGAGTGACACAGAATGTGTTTTTCTCTCCAGATCAAATTAAGCGCCTAGCCGATCGGAATTTTGGCGTTGGTT
TCGATCAGTTATTACTGGTAGAGCCTCCGTACGATCCCGATTTAGATTTTCATTATCGCATCTTTAATGCTGACGGCAGC
GAAGTTGAACAGTGTGGCAACGGTGCCCGTTGTTTTGCACGCTTTGTGCGTAATAAAGGTCTGACCCATAAACATAAAAT
TCGCGTGAGCACCTCTTCTGGTAAAATCACTTTGCGTCTTGAGCGTGACGGTAAAGTCACGGTTAATATGGGCATACCCG
TACTCGAGCCGAGCAAAATCCCTTTTACCGCTAAGAAAGTTGAGAAAACCTACCTGCTTTTGGCATCGAGCGGCACCTAT
CTTTGTGGCGCAGTTTCTATGGGTAATCCTCATTGCGTGATTGAGGTTGACGACGTGGCAGCAGCCGATGTTGATACTAT
TGGCGCCGAGCTGACCAATCATGAACGCTTTCCAAAAGGTGTGAATGTTGGCTTTATGCAGGTAGTGAATTCAGGGCATA
TTAAACTCAGAGTTTATGAGCGCGGCGCAGCTGAAACATTAGCTTGTGGTACAGGCGCCTGTGCGGCGGTGGTTGTTGGT
ATACAGCAAGGTAAACTTGATCGAAATGTGTGTGTGGACTTACCCGGTGGCAGTTTAATGATTAACTGGGACGGCGAGGG
TAAGCCTTTGTGGATGACAGGGCCTGCCGAGCATGTTTACGATGGGCAGATCGCACAATGA

Upstream 100 bases:

>100_bases
CTATGTCATTCGAGAGCGAGAAAAATTAGCTCAATTATGGCAAGGTGAGCAGATCCTGCCGTAAACTAGAATAATACTAA
GTAGCGGATGCCAACAAAGC

Downstream 100 bases:

>100_bases
TAGATAACTCGAACAAGACTCCCCCCTTCGATGAAATTCTGATCCGCGAATACTTGCTGGATAATCCAGAATTTTTTAGC
CGTTACCCAGAGCTATTGTT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MRYRYFLRMSTLIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLVEPPYDPDLDFHYRIFNADGS
EVEQCGNGARCFARFVRNKGLTHKHKIRVSTSSGKITLRLERDGKVTVNMGIPVLEPSKIPFTAKKVEKTYLLLASSGTY
LCGAVSMGNPHCVIEVDDVAAADVDTIGAELTNHERFPKGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVG
IQQGKLDRNVCVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ

Sequences:

>Translated_286_residues
MRYRYFLRMSTLIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLVEPPYDPDLDFHYRIFNADGS
EVEQCGNGARCFARFVRNKGLTHKHKIRVSTSSGKITLRLERDGKVTVNMGIPVLEPSKIPFTAKKVEKTYLLLASSGTY
LCGAVSMGNPHCVIEVDDVAAADVDTIGAELTNHERFPKGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVG
IQQGKLDRNVCVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ
>Mature_286_residues
MRYRYFLRMSTLIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLVEPPYDPDLDFHYRIFNADGS
EVEQCGNGARCFARFVRNKGLTHKHKIRVSTSSGKITLRLERDGKVTVNMGIPVLEPSKIPFTAKKVEKTYLLLASSGTY
LCGAVSMGNPHCVIEVDDVAAADVDTIGAELTNHERFPKGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVG
IQQGKLDRNVCVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=272, Percent_Identity=70.5882352941177, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31430; Mature: 31430

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYRYFLRMSTLIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLV
CCEEEEEEHHHHHHHHHHCCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEE
EPPYDPDLDFHYRIFNADGSEVEQCGNGARCFARFVRNKGLTHKHKIRVSTSSGKITLRL
CCCCCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEE
ERDGKVTVNMGIPVLEPSKIPFTAKKVEKTYLLLASSGTYLCGAVSMGNPHCVIEVDDVA
ECCCEEEEEECCCEECCCCCCCCHHCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCC
AADVDTIGAELTNHERFPKGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVG
CCCHHHHCHHHCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEECCCCCCEEEEEE
IQQGKLDRNVCVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ
CCCCCCCCCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRYRYFLRMSTLIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLV
CCEEEEEEHHHHHHHHHHCCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEE
EPPYDPDLDFHYRIFNADGSEVEQCGNGARCFARFVRNKGLTHKHKIRVSTSSGKITLRL
CCCCCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEE
ERDGKVTVNMGIPVLEPSKIPFTAKKVEKTYLLLASSGTYLCGAVSMGNPHCVIEVDDVA
ECCCEEEEEECCCEECCCCCCCCHHCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCC
AADVDTIGAELTNHERFPKGVNVGFMQVVNSGHIKLRVYERGAAETLACGTGACAAVVVG
CCCHHHHCHHHCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEECCCCCCEEEEEE
IQQGKLDRNVCVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ
CCCCCCCCCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA