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The map label for this gene is pmbA [H]

Identifier: 167625730

GI number: 167625730

Start: 4638633

End: 4640015

Strand: Reverse

Name: pmbA [H]

Synonym: Shal_3825

Alternate gene names: 167625730

Gene position: 4640015-4638633 (Counterclockwise)

Preceding gene: 167625733

Following gene: 167625728

Centisome position: 88.77

GC content: 48.08

Gene sequence:

>1383_bases
TTGTTATGCTATCGCTATTGCCGACAAATAAAGAGTAGATTTGTGTCCTCACCTAGCATTGATATTGAATTGGATTCCTT
GAAAGATGCCGTTTCGATGGCGCTGGAATATGCAAAAACATTAGGCACTTCTGGTGCAGAAGTTGCGATCAGTAAGCAGC
AAGGCTTGTCTGTTTCGACTCGAATGAAAGAAGTTGAAACCGTTGAGTTTAATAAAGATGGCGCCCTAGGCATTACCGTT
TTTAGAGACGGATGCAAAGGTAGCTCATCGACTTCAGATTTAAGTAAAGCTGCGATTCAACAAGCGGTGAAGGCGGCCGA
TGATATCGCTCGCTTCACTTCTGCGGATCCTTTTAACGGTTTGGCTGAAAAGTCGTTAATGGCGACCGAGTTCCCGGACC
TTGATCTGTACCACCCAGAAGATATTTCTCCTGAGGAGCTAACTCGCTTAGCCGTATTAGCCGAAGAAGCGGCGCTAGAT
ACTGATCCGCGAGTGCAAACTTCTGACGGTGCGAGTGCTAATGCGCACTCTAGCGTTAAAGTTTATGGCAATAGCCATGG
CTTTTTGAACGGCTATTGCAGTTCACGCTACAGCCTAAGCTGCGTCGCGATTGGTGAGCTTGAAGGCAGTATGCAGCGTG
ACTATGACTACACGATTGCACGCAAATTTAGTGAGATGACGGCGCCGGAAATTATCGGTAAGAAGGCGGCTGAAAAAACG
GCTGGCCGCTTAGGTGGGCGTAAAATAGCCACCACGCAGCTTCCTGTGCTGTTTTCACCTGAAATTGCTACAGGCTTGAT
GGGTCACTTGGTAGGAGCTATCAGTGGTGGCAGCTTATATCGTAAGTCTAGCTTCCTGCTCGATGCTATCGATACTCAAG
TGTTCCCAGATTGGTTTAGCATTCATGAAGACCCATTACTGGTCGGCGCATTGGCAAGCTCGGCATACGATAGCGAAGGC
GTTGCCACTCAGGCGCGCGATATTATCGATAATGGCCGCTTAGCAACCTACCTGCTTACCAGTTACTCGGCGCGTAAGCT
TGGGTTGACCAATACTGGCCATGCCGGTGGGATCTATAACTGGACGCTATCGACAACAGGGCAAACTTTTGACCAGTTGG
TTAAAGAGATGGGCACAGGTCTTATCGTGACAGAAGTGATGGGCCAAGGCGTGAATACGGTTACTGGTGATTACTCTCGT
GGCGCAGCGGGTTTTTATGTCGAAAATGGCGTAATTTTGTACCCAGTTGAAGAGATCACCATTGCGGGCAACCTTAAAGA
TATGTACCAAAATATCGTCGCGGTTGCTAAAGACAGAGATCTGCGTTCATCGATCCGCACTGGCGGGATCTTAATGGATA
AGATGAAGATCGCGGGTAATTAA

Upstream 100 bases:

>100_bases
TTACCGGTTTTTAGTTTCATTTAGCGGATATTTTTATACTTAAGAAGCGAGCGATAGAGCAGGTTATGAGGGTATTATTT
AATGTGATTGGGTTTTGGGT

Downstream 100 bases:

>100_bases
GGCTCCATATCTCTATTGCTATTAGTGATAGAGTGATTAGCTCTTATATAGAGCCAGCTGTAATCGACTCATCTAAAAAA
GGCTTCCAATGGGAAGCCTT

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLCYRYCRQIKSRFVSSPSIDIELDSLKDAVSMALEYAKTLGTSGAEVAISKQQGLSVSTRMKEVETVEFNKDGALGITV
FRDGCKGSSSTSDLSKAAIQQAVKAADDIARFTSADPFNGLAEKSLMATEFPDLDLYHPEDISPEELTRLAVLAEEAALD
TDPRVQTSDGASANAHSSVKVYGNSHGFLNGYCSSRYSLSCVAIGELEGSMQRDYDYTIARKFSEMTAPEIIGKKAAEKT
AGRLGGRKIATTQLPVLFSPEIATGLMGHLVGAISGGSLYRKSSFLLDAIDTQVFPDWFSIHEDPLLVGALASSAYDSEG
VATQARDIIDNGRLATYLLTSYSARKLGLTNTGHAGGIYNWTLSTTGQTFDQLVKEMGTGLIVTEVMGQGVNTVTGDYSR
GAAGFYVENGVILYPVEEITIAGNLKDMYQNIVAVAKDRDLRSSIRTGGILMDKMKIAGN

Sequences:

>Translated_460_residues
MLCYRYCRQIKSRFVSSPSIDIELDSLKDAVSMALEYAKTLGTSGAEVAISKQQGLSVSTRMKEVETVEFNKDGALGITV
FRDGCKGSSSTSDLSKAAIQQAVKAADDIARFTSADPFNGLAEKSLMATEFPDLDLYHPEDISPEELTRLAVLAEEAALD
TDPRVQTSDGASANAHSSVKVYGNSHGFLNGYCSSRYSLSCVAIGELEGSMQRDYDYTIARKFSEMTAPEIIGKKAAEKT
AGRLGGRKIATTQLPVLFSPEIATGLMGHLVGAISGGSLYRKSSFLLDAIDTQVFPDWFSIHEDPLLVGALASSAYDSEG
VATQARDIIDNGRLATYLLTSYSARKLGLTNTGHAGGIYNWTLSTTGQTFDQLVKEMGTGLIVTEVMGQGVNTVTGDYSR
GAAGFYVENGVILYPVEEITIAGNLKDMYQNIVAVAKDRDLRSSIRTGGILMDKMKIAGN
>Mature_460_residues
MLCYRYCRQIKSRFVSSPSIDIELDSLKDAVSMALEYAKTLGTSGAEVAISKQQGLSVSTRMKEVETVEFNKDGALGITV
FRDGCKGSSSTSDLSKAAIQQAVKAADDIARFTSADPFNGLAEKSLMATEFPDLDLYHPEDISPEELTRLAVLAEEAALD
TDPRVQTSDGASANAHSSVKVYGNSHGFLNGYCSSRYSLSCVAIGELEGSMQRDYDYTIARKFSEMTAPEIIGKKAAEKT
AGRLGGRKIATTQLPVLFSPEIATGLMGHLVGAISGGSLYRKSSFLLDAIDTQVFPDWFSIHEDPLLVGALASSAYDSEG
VATQARDIIDNGRLATYLLTSYSARKLGLTNTGHAGGIYNWTLSTTGQTFDQLVKEMGTGLIVTEVMGQGVNTVTGDYSR
GAAGFYVENGVILYPVEEITIAGNLKDMYQNIVAVAKDRDLRSSIRTGGILMDKMKIAGN

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=434, Percent_Identity=58.9861751152074, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49369; Mature: 49369

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCYRYCRQIKSRFVSSPSIDIELDSLKDAVSMALEYAKTLGTSGAEVAISKQQGLSVST
CCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
RMKEVETVEFNKDGALGITVFRDGCKGSSSTSDLSKAAIQQAVKAADDIARFTSADPFNG
HHHHHEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LAEKSLMATEFPDLDLYHPEDISPEELTRLAVLAEEAALDTDPRVQTSDGASANAHSSVK
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEE
VYGNSHGFLNGYCSSRYSLSCVAIGELEGSMQRDYDYTIARKFSEMTAPEIIGKKAAEKT
EEECCCCCEEEECCCCCEEEEEEEECCCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AGRLGGRKIATTQLPVLFSPEIATGLMGHLVGAISGGSLYRKSSFLLDAIDTQVFPDWFS
HHHCCCCEEEEEECCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHH
IHEDPLLVGALASSAYDSEGVATQARDIIDNGRLATYLLTSYSARKLGLTNTGHAGGIYN
CCCCCEEEEHHHHCCCCCCCCHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCCCCCEEE
WTLSTTGQTFDQLVKEMGTGLIVTEVMGQGVNTVTGDYSRGAAGFYVENGVILYPVEEIT
EEECCCCHHHHHHHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCEEEECCEEEEEHHEEE
IAGNLKDMYQNIVAVAKDRDLRSSIRTGGILMDKMKIAGN
EECCHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEEECCC
>Mature Secondary Structure
MLCYRYCRQIKSRFVSSPSIDIELDSLKDAVSMALEYAKTLGTSGAEVAISKQQGLSVST
CCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
RMKEVETVEFNKDGALGITVFRDGCKGSSSTSDLSKAAIQQAVKAADDIARFTSADPFNG
HHHHHEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LAEKSLMATEFPDLDLYHPEDISPEELTRLAVLAEEAALDTDPRVQTSDGASANAHSSVK
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEE
VYGNSHGFLNGYCSSRYSLSCVAIGELEGSMQRDYDYTIARKFSEMTAPEIIGKKAAEKT
EEECCCCCEEEECCCCCEEEEEEEECCCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AGRLGGRKIATTQLPVLFSPEIATGLMGHLVGAISGGSLYRKSSFLLDAIDTQVFPDWFS
HHHCCCCEEEEEECCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHH
IHEDPLLVGALASSAYDSEGVATQARDIIDNGRLATYLLTSYSARKLGLTNTGHAGGIYN
CCCCCEEEEHHHHCCCCCCCCHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCCCCCEEE
WTLSTTGQTFDQLVKEMGTGLIVTEVMGQGVNTVTGDYSRGAAGFYVENGVILYPVEEIT
EEECCCCHHHHHHHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCEEEECCEEEEEHHEEE
IAGNLKDMYQNIVAVAKDRDLRSSIRTGGILMDKMKIAGN
EECCHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]