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The map label for this gene is mlaE [H]

Identifier: 167625602

GI number: 167625602

Start: 4497617

End: 4498402

Strand: Reverse

Name: mlaE [H]

Synonym: Shal_3697

Alternate gene names: 167625602

Gene position: 4498402-4497617 (Counterclockwise)

Preceding gene: 167625603

Following gene: 167625601

Centisome position: 86.06

GC content: 49.62

Gene sequence:

>786_bases
GTGAGCTTGATTGATCAAATTGCTCGATTAGGTAGGGCTAGTATCGAATTTGTGATTGGGCTAGGAAAAGCCGGTTTGAT
GCTATGGGGCGCAATTGCACATCGCCCTAGGTTTCGAAAAGGTGCACCGCTGCTGTTGAGGCAGCTGTATGTTGTGGGCG
TACAGTCGATGGTGATTATCTTAGTTTCAGGCCTGTTTATCGGCATGGTACTGGCGCTGCAGGGCTATAATATTTTAGTG
GGCTTTGGCACCGAAGAAAGTTTAGGTCCCATGGTTGCTCTAAGCTTATTAAGGGAGCTAGGCCCCGTGGTGACGGCACT
GCTATTTGCTGGCCGAGCGGGCTCTGCGCTAACGGCTGAGCTTGGCCTAATGAAGAGTACCGAGCAGCTGGCGAGTCTTG
AGATGATGGCGATTGACCCACTGAGGCAGATTATTGCGCCGCGTTTTTGGGCTGGCGTCATTAGCTTGCCATTACTGGCA
TTGATGTTTACTGCTGTGGGGATCTATGGCGGACACATAGTCGGTGTTGAGTGGAAAGGCATTGATAGCGGTAGCTTCTG
GTCGATTCTTCAGGCATCAGTTGAGTGGCGAGAAGATATTGTTAATTGCATGATTAAGAGCCTACTGTTTGCGGTTGTGG
TGACATGGATAGCGCTTTATCGTGGCTACCATGTTATTCCAAATCCGGAAGGGATTAGTCGAGCGACGACTCAGACGGTA
GTACAATCTAGCCTAGCTGTTTTAGCGTTGGACTTCCTACTGACAGCGATAATGTTTGGTCAGTAG

Upstream 100 bases:

>100_bases
TTGTCAGAAGATCCAAAGTTAAAACAGTTTATTGGTGGTGAGCCTGATGGTCCGGTACCGTTTCACTACCCAGCAGAAGA
TTATAAGAAGGAACTACTCC

Downstream 100 bases:

>100_bases
TTGTATGATGATTGAGAGAGTTCCTAGTTGTTAATTATTGTTTAGATTTATTCAATAGAGTGGTAAATGAGTTATGTTGA
CAAGGAAAATTGAGATTCTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSLIDQIARLGRASIEFVIGLGKAGLMLWGAIAHRPRFRKGAPLLLRQLYVVGVQSMVIILVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAELGLMKSTEQLASLEMMAIDPLRQIIAPRFWAGVISLPLLA
LMFTAVGIYGGHIVGVEWKGIDSGSFWSILQASVEWREDIVNCMIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTV
VQSSLAVLALDFLLTAIMFGQ

Sequences:

>Translated_261_residues
MSLIDQIARLGRASIEFVIGLGKAGLMLWGAIAHRPRFRKGAPLLLRQLYVVGVQSMVIILVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAELGLMKSTEQLASLEMMAIDPLRQIIAPRFWAGVISLPLLA
LMFTAVGIYGGHIVGVEWKGIDSGSFWSILQASVEWREDIVNCMIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTV
VQSSLAVLALDFLLTAIMFGQ
>Mature_260_residues
SLIDQIARLGRASIEFVIGLGKAGLMLWGAIAHRPRFRKGAPLLLRQLYVVGVQSMVIILVSGLFIGMVLALQGYNILVG
FGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAELGLMKSTEQLASLEMMAIDPLRQIIAPRFWAGVISLPLLAL
MFTAVGIYGGHIVGVEWKGIDSGSFWSILQASVEWREDIVNCMIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTVV
QSSLAVLALDFLLTAIMFGQ

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=67.1814671814672, Blast_Score=355, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28168; Mature: 28037

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIDQIARLGRASIEFVIGLGKAGLMLWGAIAHRPRFRKGAPLLLRQLYVVGVQSMVII
CCHHHHHHHHCCHHHHHHEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LGLMKSTEQLASLEMMAIDPLRQIIAPRFWAGVISLPLLALMFTAVGIYGGHIVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IDSGSFWSILQASVEWREDIVNCMIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VQSSLAVLALDFLLTAIMFGQ
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLIDQIARLGRASIEFVIGLGKAGLMLWGAIAHRPRFRKGAPLLLRQLYVVGVQSMVII
CHHHHHHHHCCHHHHHHEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LGLMKSTEQLASLEMMAIDPLRQIIAPRFWAGVISLPLLALMFTAVGIYGGHIVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IDSGSFWSILQASVEWREDIVNCMIKSLLFAVVVTWIALYRGYHVIPNPEGISRATTQTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VQSSLAVLALDFLLTAIMFGQ
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]