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The map label for this gene is cysN [H]

Identifier: 167625440

GI number: 167625440

Start: 4307812

End: 4309251

Strand: Reverse

Name: cysN [H]

Synonym: Shal_3532

Alternate gene names: 167625440

Gene position: 4309251-4307812 (Counterclockwise)

Preceding gene: 167625441

Following gene: 167625439

Centisome position: 82.44

GC content: 47.85

Gene sequence:

>1440_bases
ATGAATCAAGCAGTAGATAACAACGCGCGCCTGGCGGCAGAACTTGAAGATTTAGGGGTAAAAGAATACCTGTCATTGCA
GCAGCATAAAGGGCTATTACGCTTCTTAACTTGCGGCAGTGTTGACGATGGCAAGAGTACCTTGATTGGGCGCTTGTTGC
ATGACAGCGCCCAAATTTATGAAGACCAATTGGCGAGCCTTAAAAATGATAGCGCTAAAGTGGGCACCACAGGTGAAGAG
ATCGACTTAGCACTGCTGGTTGATGGCTTACAAGCGGAGCGTGAGCAGGGCATTACTATCGATGTGGCTTACCGTTACTT
TTCCAGTGACAAACGTAAGTTTATTATTGCCGATACGCCGGGTCATGAGCAGTACACGCGTAACATGGCCACGGGCGCCT
CTACCTGTGATTTGGCCGTGCTCTTAGTCGATGCGCGCTATGGCGTACAAACTCAAACTCGTCGTCATGCATTTATTGCA
TCACAGCTAGGTATTCGCCACTTTGTTGTTGCTATCAACAAGATGGATCTACTCGGGTTTGATGAGAAAGTCTTTAACGA
CATTCAAGCCGAGTTTGCAGCCTTTGCCGAGCAGTTAACTGATATCGATATTCGCTATGTGCCGCTGTCAGCGCTTAAAG
GTGACAACGTGGTTGATGCCAGCAGTCAAACCCCTTGGTATAGCGGCGGTACGCTGTTAGAGCTGTTAGAAACTATCGAT
ACTCGCCGTGAGCTTAACAGCTTACCTGTACGTTTCCCGGTGCAATATGTGCAGCGTCCCGATCTCGATTTTCGTGGTTT
TTCAGGCACGTTAGCATCGGGTGTGATTAACGTCGGTGACGAGCTGGTGGCTCTGCCATCGGGCAAGCGCAGTAAAGTTG
CCAGTATCGTGACCTTCGATGGCTTGCTTGAGCAAGCGATTGCAGGCCAAGCGGTGACCTTAACTCTTGAAGATGAAATT
GATATCTCAAGGGGCGATCTGTTATCGCAAGTCGATAATCCACCTGCACTGGCTAACCATGTTAGCGCCGATATCGCTTG
GATGGATGAAAAGCCACTGCAGCTAGGCCAGCTTTACGATCTTAAAGTTGCAGGTAAAAAGCTGCAAGCCAGCGTGGTAA
AAATTGAATATGTTGTCGATGTTAATACCTTAGAGTGCAATGACAGCAGCAGTATTTGCCTTAACGATATGGCGCGAGTC
ACACTTGAGTTTAATGAGTCGATTGCTATCGACCCATACAGCTTAATCCGCGATACCGGCGGCATGATCTTAATCGATCG
CTTATCTAACGCGACTGTTGCAGCTGTGATGGCTGTATCGAGCAGCAAAGTGCAAAAAACGGCTCAGCAATACAGTAAAT
TTGAACTGGAGCTGAATGCATTAATTCGCAAGCAATATCCTCACTGGCAAGCGATAGATATTAGCAAGGTTGAGGGCTAG

Upstream 100 bases:

>100_bases
CTCAGATCAGAGTGCATCGATGGAACTGAAAAAACGTCAGGGCTATTTTTAAGCGTACCCATCCGTGATGAATATGGACT
AAGAATTCGAAGGAATTATT

Downstream 100 bases:

>100_bases
CTATGGCCGATTTGTGGGTGTTGTCGTGCATATTATTTGCCTTAGTCGCCGCCTTGATGGCGGGGCTATGGACACCTGCA
GCGCTATTTTTTATTGCGTC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNQAVDNNARLAAELEDLGVKEYLSLQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKNDSAKVGTTGEE
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIA
SQLGIRHFVVAINKMDLLGFDEKVFNDIQAEFAAFAEQLTDIDIRYVPLSALKGDNVVDASSQTPWYSGGTLLELLETID
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVASIVTFDGLLEQAIAGQAVTLTLEDEI
DISRGDLLSQVDNPPALANHVSADIAWMDEKPLQLGQLYDLKVAGKKLQASVVKIEYVVDVNTLECNDSSSICLNDMARV
TLEFNESIAIDPYSLIRDTGGMILIDRLSNATVAAVMAVSSSKVQKTAQQYSKFELELNALIRKQYPHWQAIDISKVEG

Sequences:

>Translated_479_residues
MNQAVDNNARLAAELEDLGVKEYLSLQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKNDSAKVGTTGEE
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIA
SQLGIRHFVVAINKMDLLGFDEKVFNDIQAEFAAFAEQLTDIDIRYVPLSALKGDNVVDASSQTPWYSGGTLLELLETID
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVASIVTFDGLLEQAIAGQAVTLTLEDEI
DISRGDLLSQVDNPPALANHVSADIAWMDEKPLQLGQLYDLKVAGKKLQASVVKIEYVVDVNTLECNDSSSICLNDMARV
TLEFNESIAIDPYSLIRDTGGMILIDRLSNATVAAVMAVSSSKVQKTAQQYSKFELELNALIRKQYPHWQAIDISKVEG
>Mature_479_residues
MNQAVDNNARLAAELEDLGVKEYLSLQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIYEDQLASLKNDSAKVGTTGEE
IDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIA
SQLGIRHFVVAINKMDLLGFDEKVFNDIQAEFAAFAEQLTDIDIRYVPLSALKGDNVVDASSQTPWYSGGTLLELLETID
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVASIVTFDGLLEQAIAGQAVTLTLEDEI
DISRGDLLSQVDNPPALANHVSADIAWMDEKPLQLGQLYDLKVAGKKLQASVVKIEYVVDVNTLECNDSSSICLNDMARV
TLEFNESIAIDPYSLIRDTGGMILIDRLSNATVAAVMAVSSSKVQKTAQQYSKFELELNALIRKQYPHWQAIDISKVEG

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=426, Percent_Identity=29.3427230046948, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI5729864, Length=426, Percent_Identity=29.3427230046948, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI4503475, Length=464, Percent_Identity=26.2931034482759, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI4503471, Length=360, Percent_Identity=28.8888888888889, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI194097354, Length=311, Percent_Identity=29.5819935691318, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI194018520, Length=311, Percent_Identity=29.5819935691318, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI194018522, Length=311, Percent_Identity=29.5819935691318, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI46094014, Length=311, Percent_Identity=29.2604501607717, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI34147630, Length=301, Percent_Identity=31.5614617940199, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157426893, Length=353, Percent_Identity=26.3456090651558, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1789108, Length=459, Percent_Identity=61.6557734204793, Blast_Score=570, Evalue=1e-163,
Organism=Escherichia coli, GI1790412, Length=313, Percent_Identity=26.1980830670927, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789737, Length=313, Percent_Identity=26.1980830670927, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17552884, Length=360, Percent_Identity=30.5555555555556, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=360, Percent_Identity=30.5555555555556, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=438, Percent_Identity=26.9406392694064, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=296, Percent_Identity=29.7297297297297, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=296, Percent_Identity=29.7297297297297, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI32566303, Length=353, Percent_Identity=28.6118980169972, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.9565217391304, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=207, Percent_Identity=29.951690821256, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=351, Percent_Identity=25.6410256410256, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325337, Length=333, Percent_Identity=30.6306306306306, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=333, Percent_Identity=30.6306306306306, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=336, Percent_Identity=31.8452380952381, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320377, Length=347, Percent_Identity=29.3948126801153, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324761, Length=283, Percent_Identity=28.9752650176678, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=192, Percent_Identity=31.25, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550900, Length=426, Percent_Identity=27.9342723004695, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24652838, Length=463, Percent_Identity=25.9179265658747, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17137572, Length=463, Percent_Identity=25.9179265658747, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45553807, Length=360, Percent_Identity=27.2222222222222, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI45553816, Length=360, Percent_Identity=27.2222222222222, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24651721, Length=360, Percent_Identity=27.2222222222222, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17864154, Length=360, Percent_Identity=27.2222222222222, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17137380, Length=314, Percent_Identity=28.0254777070064, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI281363316, Length=312, Percent_Identity=30.1282051282051, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI17864358, Length=312, Percent_Identity=30.1282051282051, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19921738, Length=304, Percent_Identity=29.2763157894737, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52638; Mature: 52638

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAVDNNARLAAELEDLGVKEYLSLQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEHHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKNDSAKVGTTGEEIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIASQLGIRHFVVAINKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEEECHHCCCC
DEKVFNDIQAEFAAFAEQLTDIDIRYVPLSALKGDNVVDASSQTPWYSGGTLLELLETID
CHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHH
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVASIVTFD
HHHHHHCCCCEECHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHH
GLLEQAIAGQAVTLTLEDEIDISRGDLLSQVDNPPALANHVSADIAWMDEKPLQLGQLYD
HHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCCHHHHHCCCCEEECCCCCCCCCCEEE
LKVAGKKLQASVVKIEYVVDVNTLECNDSSSICLNDMARVTLEFNESIAIDPYSLIRDTG
EEECCCHHEEEEEEEEEEEEEEEEEECCCCCEEECCCEEEEEEECCEEECCCHHHHHCCC
GMILIDRLSNATVAAVMAVSSSKVQKTAQQYSKFELELNALIRKQYPHWQAIDISKVEG
CEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
>Mature Secondary Structure
MNQAVDNNARLAAELEDLGVKEYLSLQQHKGLLRFLTCGSVDDGKSTLIGRLLHDSAQIY
CCCCCCCCCEEEHHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
EDQLASLKNDSAKVGTTGEEIDLALLVDGLQAEREQGITIDVAYRYFSSDKRKFIIADTP
HHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECC
GHEQYTRNMATGASTCDLAVLLVDARYGVQTQTRRHAFIASQLGIRHFVVAINKMDLLGF
CCHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEEECHHCCCC
DEKVFNDIQAEFAAFAEQLTDIDIRYVPLSALKGDNVVDASSQTPWYSGGTLLELLETID
CHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHH
TRRELNSLPVRFPVQYVQRPDLDFRGFSGTLASGVINVGDELVALPSGKRSKVASIVTFD
HHHHHHCCCCEECHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHH
GLLEQAIAGQAVTLTLEDEIDISRGDLLSQVDNPPALANHVSADIAWMDEKPLQLGQLYD
HHHHHHHCCCEEEEEEECCCCCCCCHHHHHCCCCCHHHHHCCCCEEECCCCCCCCCCEEE
LKVAGKKLQASVVKIEYVVDVNTLECNDSSSICLNDMARVTLEFNESIAIDPYSLIRDTG
EEECCCHHEEEEEEEEEEEEEEEEEECCCCCEEECCCEEEEEEECCEEECCCHHHHHCCC
GMILIDRLSNATVAAVMAVSSSKVQKTAQQYSKFELELNALIRKQYPHWQAIDISKVEG
CEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]