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The map label for this gene is ubiH [H]

Identifier: 167625288

GI number: 167625288

Start: 4120997

End: 4122349

Strand: Reverse

Name: ubiH [H]

Synonym: Shal_3377

Alternate gene names: 167625288

Gene position: 4122349-4120997 (Counterclockwise)

Preceding gene: 167625289

Following gene: 167625287

Centisome position: 78.87

GC content: 51.07

Gene sequence:

>1353_bases
ATGGCTAATCCTAACGAATGCTCTGATGTAGCGAACATAACAGCTCCTCTCTCTGCGGCAGCGGCTACTGAGATCAATCA
AGTCGACATCGCCATTGTCGGCGGTGCAATGGCGGGGGCAACCTTGGCCTTGGGGCTGGCCCAGCTTACAAAAAAAATTA
CCAAAGAGCTTGGCCAAAGCGCTGAACAAAAGCCACTTAAAATCGCCTTAATCGAGGCCCACAAGCCAAGTGATGAACAC
CCAGGGTTTGATGCCCGCTCAATCGCTATCTCCCATGGCTCTATCTTCCAGTTACAGCAGCTAGATCTGTGGCAGCACCT
CGAGTCATTGGCAACGCCCATTAGCAATATTCATGTATCAGATCGTGGCCACTTCGGCATGACAGAGCTTAACAGCGAGT
CGTTCCATCTGCCGTACTTAGGCCAAGTGGTCGAGCTTGAGGCGGTGGGTAACAAGCTGTTTAGTCTGCTAGAAAAAAGT
GATATCGCCCTCTATTGCCCCAATAAGTTGCAAAGTGTCGAGGCGCGCATGGACTCACAACTGCTGACCCTCGAAGATGG
CCAGCAGATCGCAGCTAAATTGGTTGTTGCTGCCGATGGCGTTAACTCCAAGGTACGCCAAGCCTTTAAGCTACCACTTG
AGCAGATTGATTTTGGCCAGAGCGCGATTATCACCAATATTCACACCGAGCAGCCACATCAAGGCTGGGCCTATGAGCGC
TTTACCCAAACCGGCCCATTGGCATTGCTGCCAATGGCAGACAGCCAGAGCGATTCACGCCTCTCTTTAGTGTGGGCATT
ACCGCCAGCAGATGCCGAGCGACTATTAACCGCACCTAGAGCGGAATTTATTGAGCAACTACAGCAAGCCTTTGGCTATC
GAGCCGGGCGCTTTATCGATATCGGCAACCGTTTTAGTTACCCGCTATTGTTATCGTATATGCCACGGCCTATCTACCAT
CGCACGGTATTTTTGGGCAACGCGGCGCAAACCCTGCATCCGATTGCGGGCCAAGGCTTTAACTTAGGCCTACGCGATGT
GGTGTGCTTGATTGACGTTATTGAAAAAGAGTTAACTAAGTTAGCGCTTGCTAACTCAGAATGTACTAACCCAGAAGGTG
AGTCACAACCGACTGTCGATCTTGGCAGTGCAGCGTTAACCCATGCCTACCTTGAGCGCCGTAACGCCGACAGGCAAGGC
ACTATGAACAGTATTGAATTCTTAGTGCGCGGATTTTCAAATGATCATTGGCCATTAGTCATTGGCCGTAACCTTGGGCT
GCGATTGCTGTCTTGGTTGCCATCACTTAAAACACCGGTGGCGCAAAAGGCGATGGGCTGGATCCAAAGCTAG

Upstream 100 bases:

>100_bases
ACTAAAACTCGTACCCAATACCGTCATCGCAGTTATTTGCCATGGCGGTTTTTGTATCAGCAACACTACCAACTAAATTT
GTTATTGGGTCAATAAACTC

Downstream 100 bases:

>100_bases
CGACTCGCGTGGTTGGACATTCTACGCGCCAGTTAAACACTAGGGTTAATCGTGTTTTTTAGGTCAAAAGCCGTTTTTAT
TGAGTAAGGGTACAAGATGT

Product: 2-polyprenyl-6-methoxyphenol 4-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MANPNECSDVANITAPLSAAAATEINQVDIAIVGGAMAGATLALGLAQLTKKITKELGQSAEQKPLKIALIEAHKPSDEH
PGFDARSIAISHGSIFQLQQLDLWQHLESLATPISNIHVSDRGHFGMTELNSESFHLPYLGQVVELEAVGNKLFSLLEKS
DIALYCPNKLQSVEARMDSQLLTLEDGQQIAAKLVVAADGVNSKVRQAFKLPLEQIDFGQSAIITNIHTEQPHQGWAYER
FTQTGPLALLPMADSQSDSRLSLVWALPPADAERLLTAPRAEFIEQLQQAFGYRAGRFIDIGNRFSYPLLLSYMPRPIYH
RTVFLGNAAQTLHPIAGQGFNLGLRDVVCLIDVIEKELTKLALANSECTNPEGESQPTVDLGSAALTHAYLERRNADRQG
TMNSIEFLVRGFSNDHWPLVIGRNLGLRLLSWLPSLKTPVAQKAMGWIQS

Sequences:

>Translated_450_residues
MANPNECSDVANITAPLSAAAATEINQVDIAIVGGAMAGATLALGLAQLTKKITKELGQSAEQKPLKIALIEAHKPSDEH
PGFDARSIAISHGSIFQLQQLDLWQHLESLATPISNIHVSDRGHFGMTELNSESFHLPYLGQVVELEAVGNKLFSLLEKS
DIALYCPNKLQSVEARMDSQLLTLEDGQQIAAKLVVAADGVNSKVRQAFKLPLEQIDFGQSAIITNIHTEQPHQGWAYER
FTQTGPLALLPMADSQSDSRLSLVWALPPADAERLLTAPRAEFIEQLQQAFGYRAGRFIDIGNRFSYPLLLSYMPRPIYH
RTVFLGNAAQTLHPIAGQGFNLGLRDVVCLIDVIEKELTKLALANSECTNPEGESQPTVDLGSAALTHAYLERRNADRQG
TMNSIEFLVRGFSNDHWPLVIGRNLGLRLLSWLPSLKTPVAQKAMGWIQS
>Mature_449_residues
ANPNECSDVANITAPLSAAAATEINQVDIAIVGGAMAGATLALGLAQLTKKITKELGQSAEQKPLKIALIEAHKPSDEHP
GFDARSIAISHGSIFQLQQLDLWQHLESLATPISNIHVSDRGHFGMTELNSESFHLPYLGQVVELEAVGNKLFSLLEKSD
IALYCPNKLQSVEARMDSQLLTLEDGQQIAAKLVVAADGVNSKVRQAFKLPLEQIDFGQSAIITNIHTEQPHQGWAYERF
TQTGPLALLPMADSQSDSRLSLVWALPPADAERLLTAPRAEFIEQLQQAFGYRAGRFIDIGNRFSYPLLLSYMPRPIYHR
TVFLGNAAQTLHPIAGQGFNLGLRDVVCLIDVIEKELTKLALANSECTNPEGESQPTVDLGSAALTHAYLERRNADRQGT
MNSIEFLVRGFSNDHWPLVIGRNLGLRLLSWLPSLKTPVAQKAMGWIQS

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=404, Percent_Identity=25.7425742574257, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI32967307, Length=330, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1789274, Length=424, Percent_Identity=42.2169811320755, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1789273, Length=426, Percent_Identity=30.9859154929577, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1786883, Length=420, Percent_Identity=30.7142857142857, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25146631, Length=456, Percent_Identity=26.3157894736842, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19920760, Length=471, Percent_Identity=24.416135881104, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 49249; Mature: 49118

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPNECSDVANITAPLSAAAATEINQVDIAIVGGAMAGATLALGLAQLTKKITKELGQS
CCCCHHHHHHHHHHHCHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
AEQKPLKIALIEAHKPSDEHPGFDARSIAISHGSIFQLQQLDLWQHLESLATPISNIHVS
HHCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCHHHEEEC
DRGHFGMTELNSESFHLPYLGQVVELEAVGNKLFSLLEKSDIALYCPNKLQSVEARMDSQ
CCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHCCC
LLTLEDGQQIAAKLVVAADGVNSKVRQAFKLPLEQIDFGQSAIITNIHTEQPHQGWAYER
EEEECCCHHHHHHHEEEECCCCHHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCHHH
FTQTGPLALLPMADSQSDSRLSLVWALPPADAERLLTAPRAEFIEQLQQAFGYRAGRFID
HCCCCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCEEE
IGNRFSYPLLLSYMPRPIYHRTVFLGNAAQTLHPIAGQGFNLGLRDVVCLIDVIEKELTK
CCCCCCCHHHHHHCCCCHHEEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LALANSECTNPEGESQPTVDLGSAALTHAYLERRNADRQGTMNSIEFLVRGFSNDHWPLV
HHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEE
IGRNLGLRLLSWLPSLKTPVAQKAMGWIQS
EECCCHHHHHHHCCHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
ANPNECSDVANITAPLSAAAATEINQVDIAIVGGAMAGATLALGLAQLTKKITKELGQS
CCCHHHHHHHHHHHCHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
AEQKPLKIALIEAHKPSDEHPGFDARSIAISHGSIFQLQQLDLWQHLESLATPISNIHVS
HHCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCHHHEEEC
DRGHFGMTELNSESFHLPYLGQVVELEAVGNKLFSLLEKSDIALYCPNKLQSVEARMDSQ
CCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHCCC
LLTLEDGQQIAAKLVVAADGVNSKVRQAFKLPLEQIDFGQSAIITNIHTEQPHQGWAYER
EEEECCCHHHHHHHEEEECCCCHHHHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCHHH
FTQTGPLALLPMADSQSDSRLSLVWALPPADAERLLTAPRAEFIEQLQQAFGYRAGRFID
HCCCCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCEEE
IGNRFSYPLLLSYMPRPIYHRTVFLGNAAQTLHPIAGQGFNLGLRDVVCLIDVIEKELTK
CCCCCCCHHHHHHCCCCHHEEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LALANSECTNPEGESQPTVDLGSAALTHAYLERRNADRQGTMNSIEFLVRGFSNDHWPLV
HHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEE
IGRNLGLRLLSWLPSLKTPVAQKAMGWIQS
EECCCHHHHHHHCCHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]