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The map label for this gene is gshB [H]

Identifier: 167625209

GI number: 167625209

Start: 4019989

End: 4021008

Strand: Reverse

Name: gshB [H]

Synonym: Shal_3297

Alternate gene names: 167625209

Gene position: 4021008-4019989 (Counterclockwise)

Preceding gene: 167625210

Following gene: 167625208

Centisome position: 76.93

GC content: 43.33

Gene sequence:

>1020_bases
ATGTACCCTTGGACGAGTATCGAGCCTGAAACCGATACCACACTGCGTTTAATTCACGAATGTGTTGCTCGTGGGCACAC
AGTAGCGCTTGCGACGACTAGCAACCTAACTATGCGTGATTCAGAAGCCAGTGCGTTCTGCCAAGTGTTCGATAAAGAAC
AAGTTACGCCAAATAATATTGTTTCATTCTATAAAAAAGCGCAGTTCAAAAAGCTACGTTTGCCGTTAGCTGGCTTTGAC
AGCATTATCATGCGCGCCAATCCACCATTGGATCCGATTGCGCTTAACTTCTTAGATTCGGTGCGTGATGAAGTGTTCAT
CATGAATGACATTGACGGCTTACGTATTGCAAATAACAAGCTATACACAGCGTCATTCGATGATCCAAATAACACCTTTA
TTCCTGTTACCCATGTTTCTAAAACCCCTGAATATCTAGAAGAAGTGCTACGTGAGTCTAAGACTGACAAGATGATCATG
AAGCCTCTTGATGGTTACGGTGGTCAGGGCGTGATCTTGGTTGAGAAAGCGGCTCAAAATAGCTTCCACTCACTGCTTGA
GTTCTACATCAACAGTGGCAAAGGCGGTAACTACGTAATTTTACAAGAGTATGTAGAAGGCGCAGATGAAGGCGATGTAC
GTATTCTTATGCTAAACGGCGAGCCTATTGGTGCGATGAAGCGTATTCCTGCACAAGGTGAGGTTCGCTCAAACATTCAT
GCCGGTGGCAGTGTCGTTAAGCATGTACTGACCAAGAAAGAAAAAGAGCTGTGTAAGCACATTGGTCCTAAGCTGGTGCG
TGACGGTTTGTTCTTTGTCGGTATTGATGTGATTAACGAAAAGCTGATTGAAGTAAACGTACAAAGCCCTGGTGGCATTA
TGCGTATCAACAAACTGAATAAGGTTAAGCTTCAGACTAAAGTTATTGATTTTGTTGAAAGTGTTGTTAATGCCAAAGAG
GTGTTAACTCAGAGGCGATCAGAATTTAGAAAGGCGATTGACGATGCTCACATTATCTGA

Upstream 100 bases:

>100_bases
TTTATTTTGAATACTCTACACCTCCCCAAGCCATCATCCGGCCCAAGATCTTTAGAGCATTCCACTTTTTTAAGAGATCC
TATTGAATATTTTATTTTTG

Downstream 100 bases:

>100_bases
GAAAGAGTGTATTGCGTTAATTAATAAAGGCGAATGCTTTCAAGCTGAAGTGGAAGGTGGCTCATTTATCGTAAAGATTG
ATGAGTACTCTCCGGTCGTA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MYPWTSIEPETDTTLRLIHECVARGHTVALATTSNLTMRDSEASAFCQVFDKEQVTPNNIVSFYKKAQFKKLRLPLAGFD
SIIMRANPPLDPIALNFLDSVRDEVFIMNDIDGLRIANNKLYTASFDDPNNTFIPVTHVSKTPEYLEEVLRESKTDKMIM
KPLDGYGGQGVILVEKAAQNSFHSLLEFYINSGKGGNYVILQEYVEGADEGDVRILMLNGEPIGAMKRIPAQGEVRSNIH
AGGSVVKHVLTKKEKELCKHIGPKLVRDGLFFVGIDVINEKLIEVNVQSPGGIMRINKLNKVKLQTKVIDFVESVVNAKE
VLTQRRSEFRKAIDDAHII

Sequences:

>Translated_339_residues
MYPWTSIEPETDTTLRLIHECVARGHTVALATTSNLTMRDSEASAFCQVFDKEQVTPNNIVSFYKKAQFKKLRLPLAGFD
SIIMRANPPLDPIALNFLDSVRDEVFIMNDIDGLRIANNKLYTASFDDPNNTFIPVTHVSKTPEYLEEVLRESKTDKMIM
KPLDGYGGQGVILVEKAAQNSFHSLLEFYINSGKGGNYVILQEYVEGADEGDVRILMLNGEPIGAMKRIPAQGEVRSNIH
AGGSVVKHVLTKKEKELCKHIGPKLVRDGLFFVGIDVINEKLIEVNVQSPGGIMRINKLNKVKLQTKVIDFVESVVNAKE
VLTQRRSEFRKAIDDAHII
>Mature_339_residues
MYPWTSIEPETDTTLRLIHECVARGHTVALATTSNLTMRDSEASAFCQVFDKEQVTPNNIVSFYKKAQFKKLRLPLAGFD
SIIMRANPPLDPIALNFLDSVRDEVFIMNDIDGLRIANNKLYTASFDDPNNTFIPVTHVSKTPEYLEEVLRESKTDKMIM
KPLDGYGGQGVILVEKAAQNSFHSLLEFYINSGKGGNYVILQEYVEGADEGDVRILMLNGEPIGAMKRIPAQGEVRSNIH
AGGSVVKHVLTKKEKELCKHIGPKLVRDGLFFVGIDVINEKLIEVNVQSPGGIMRINKLNKVKLQTKVIDFVESVVNAKE
VLTQRRSEFRKAIDDAHII

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=319, Percent_Identity=31.0344827586207, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787076, Length=266, Percent_Identity=24.812030075188, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 37944; Mature: 37944

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPWTSIEPETDTTLRLIHECVARGHTVALATTSNLTMRDSEASAFCQVFDKEQVTPNNI
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEECCHHHHHHHHHCCCCCCHHHH
VSFYKKAQFKKLRLPLAGFDSIIMRANPPLDPIALNFLDSVRDEVFIMNDIDGLRIANNK
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCEEEECCE
LYTASFDDPNNTFIPVTHVSKTPEYLEEVLRESKTDKMIMKPLDGYGGQGVILVEKAAQN
EEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEHHHHH
SFHSLLEFYINSGKGGNYVILQEYVEGADEGDVRILMLNGEPIGAMKRIPAQGEVRSNIH
HHHHHHHHHHCCCCCCCEEEEEHHHCCCCCCCEEEEEECCCCCCHHHCCCCCCCHHHHHC
AGGSVVKHVLTKKEKELCKHIGPKLVRDGLFFVGIDVINEKLIEVNVQSPGGIMRINKLN
CCHHHHHHHHHHHHHHHHHHHCHHHHHCCHHEEEHHHHCCEEEEEEECCCCCEEEECCCC
KVKLQTKVIDFVESVVNAKEVLTQRRSEFRKAIDDAHII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYPWTSIEPETDTTLRLIHECVARGHTVALATTSNLTMRDSEASAFCQVFDKEQVTPNNI
CCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEECCHHHHHHHHHCCCCCCHHHH
VSFYKKAQFKKLRLPLAGFDSIIMRANPPLDPIALNFLDSVRDEVFIMNDIDGLRIANNK
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCEEEECCE
LYTASFDDPNNTFIPVTHVSKTPEYLEEVLRESKTDKMIMKPLDGYGGQGVILVEKAAQN
EEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEHHHHH
SFHSLLEFYINSGKGGNYVILQEYVEGADEGDVRILMLNGEPIGAMKRIPAQGEVRSNIH
HHHHHHHHHHCCCCCCCEEEEEHHHCCCCCCCEEEEEECCCCCCHHHCCCCCCCHHHHHC
AGGSVVKHVLTKKEKELCKHIGPKLVRDGLFFVGIDVINEKLIEVNVQSPGGIMRINKLN
CCHHHHHHHHHHHHHHHHHHHCHHHHHCCHHEEEHHHHCCEEEEEEECCCCCEEEECCCC
KVKLQTKVIDFVESVVNAKEVLTQRRSEFRKAIDDAHII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]