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The map label for this gene is carA [H]

Identifier: 167625074

GI number: 167625074

Start: 3861547

End: 3862692

Strand: Reverse

Name: carA [H]

Synonym: Shal_3162

Alternate gene names: 167625074

Gene position: 3862692-3861547 (Counterclockwise)

Preceding gene: 167625075

Following gene: 167625073

Centisome position: 73.9

GC content: 43.37

Gene sequence:

>1146_bases
TTGACAAAGTCTGCCCTACTCGTACTTGAAGATGGAACCATTTTTTCTGGCACTGCAATTGGTGCTGAAGGGATGTCTGT
TGGTGAAGTCGTTTTTAACACTTCAATGACAGGTTACCAAGAAATTTTGACGGATCCATCATACTCACGTCAAATGGTAA
CCCTCACTTATCCACATATTGGTAATACTGGTACCAATGAAGAAGATACAGAATCTGATTCTGTTCATGCATGTGGTCTT
ATTATTAGAGACTTGCCGTTAATCGCAAGTAATTTTCGTAATCAGCAATCTTTAAGCGATTATTTGATCGCTAACAACGT
TGTTGGCATTGCTGATATCGATACTCGTAAATTAACTCGTATCCTACGTGAAAAAGGCGCTCAAGCCGGTTGTATCATGG
TTGGTGATTTAGATGAAGCTAAAGCGCTAGCTGCCGCTAAAGCCTTCCCTGGTCTTAAAGGCATGGATCTCGCTAAAGAA
GTGACAACAAACGAAACCTATTCATGGCGCAGCGGTAGCTGGCGTTTAGTTGGCGGTTTGCCATCTGATACGCCTGAAGC
TGAGCTTAAATATAAAGTTGTTGCTTATGACTATGGCGTGAAGCGTAATATCTTACGTATGTTGGTTGACCGTGGCTGTG
ATGTCACTGTTGTACCTGCACAAACTCCTGCAGCCGAAGTACTTGCAATGAATCCAGATGGGGTTTTCCTATCGAATGGC
CCTGGTGACCCTGAGCCATGTGATTACGCAATTACGGCTATTCAAGAGATCTTAAAAACAGAGATCCCTGTTTTTGGTAT
CTGTTTAGGTCACCAGCTGTTGGCATTAGCTTCAGGTGCGAAAACACTGAAAATGAAGTTTGGTCATCACGGTGCCAACC
ATCCTGTGAGTAATATTGAGCAAGGAAATGTGATGATCACTAGCCAAAACCACGGTTTTGCTGCTGATGAAACAACACTA
CCAGCCAATATTAAAGTGACTCACAAGTCACTATTTGATGGCTCATTGCAAGGTATTCACCTGACCGATAAGCCAGCATT
TAGTTTCCAAGGACACCCTGAGGCAAGCCCAGGACCACATGATGCCGCACCGTTGTTTAATCACTTCATCGATTTGATTG
AATTGTACCGCAAGAACGCCAAGTAG

Upstream 100 bases:

>100_bases
GCGGAATTTGTCAAAATTTCGTATTTTAGCGAAAGTTGGTATTTTAGAAACACTCAAAAAACAATTAAATTCAAAGGTTT
GGCTCTTTTCGGAGGTCGCA

Downstream 100 bases:

>100_bases
TTACCTCATCCGGGAGAAGATTTAAGCAATGCCAAAACGTACAGATATAAAGAGTATTCTTATCCTAGGTGCTGGACCGA
TTGTAATTGGTCAAGCCTGT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQMVTLTYPHIGNTGTNEEDTESDSVHACGL
IIRDLPLIASNFRNQQSLSDYLIANNVVGIADIDTRKLTRILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKE
VTTNETYSWRSGSWRLVGGLPSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNG
PGDPEPCDYAITAIQEILKTEIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEQGNVMITSQNHGFAADETTL
PANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPHDAAPLFNHFIDLIELYRKNAK

Sequences:

>Translated_381_residues
MTKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQMVTLTYPHIGNTGTNEEDTESDSVHACGL
IIRDLPLIASNFRNQQSLSDYLIANNVVGIADIDTRKLTRILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKE
VTTNETYSWRSGSWRLVGGLPSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNG
PGDPEPCDYAITAIQEILKTEIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEQGNVMITSQNHGFAADETTL
PANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPHDAAPLFNHFIDLIELYRKNAK
>Mature_380_residues
TKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQMVTLTYPHIGNTGTNEEDTESDSVHACGLI
IRDLPLIASNFRNQQSLSDYLIANNVVGIADIDTRKLTRILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKEV
TTNETYSWRSGSWRLVGGLPSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNGP
GDPEPCDYAITAIQEILKTEIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEQGNVMITSQNHGFAADETTLP
ANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPHDAAPLFNHFIDLIELYRKNAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=381, Percent_Identity=37.007874015748, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI169790915, Length=384, Percent_Identity=35.4166666666667, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI21361331, Length=384, Percent_Identity=35.4166666666667, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=77.7486910994764, Blast_Score=622, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI193204318, Length=383, Percent_Identity=37.0757180156658, Blast_Score=230, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6324878, Length=388, Percent_Identity=38.9175257731959, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=400, Percent_Identity=35.25, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322638, Length=162, Percent_Identity=29.0123456790123, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=38.2428940568475, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=38.2428940568475, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41011; Mature: 40880

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQMVTLTYPHI
CCCCEEEEEECCCEEECCEECCCCCCHHHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNEEDTESDSVHACGLIIRDLPLIASNFRNQQSLSDYLIANNVVGIADIDTRKLTR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCHHHHHH
ILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKEVTTNETYSWRSGSWRLVGGL
HHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCCCEEEECCC
PSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNG
CCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEECCC
PGDPEPCDYAITAIQEILKTEIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIE
CCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
QGNVMITSQNHGFAADETTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPH
CCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCC
DAAPLFNHFIDLIELYRKNAK
CHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKSALLVLEDGTIFSGTAIGAEGMSVGEVVFNTSMTGYQEILTDPSYSRQMVTLTYPHI
CCCEEEEEECCCEEECCEECCCCCCHHHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNEEDTESDSVHACGLIIRDLPLIASNFRNQQSLSDYLIANNVVGIADIDTRKLTR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEECCHHHHHH
ILREKGAQAGCIMVGDLDEAKALAAAKAFPGLKGMDLAKEVTTNETYSWRSGSWRLVGGL
HHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCCCEEEECCC
PSDTPEAELKYKVVAYDYGVKRNILRMLVDRGCDVTVVPAQTPAAEVLAMNPDGVFLSNG
CCCCCCCCEEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEECCC
PGDPEPCDYAITAIQEILKTEIPVFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIE
CCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
QGNVMITSQNHGFAADETTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPH
CCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCC
DAAPLFNHFIDLIELYRKNAK
CHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]