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The map label for this gene is rrmJ [H]

Identifier: 167625067

GI number: 167625067

Start: 3852269

End: 3852898

Strand: Reverse

Name: rrmJ [H]

Synonym: Shal_3155

Alternate gene names: 167625067

Gene position: 3852898-3852269 (Counterclockwise)

Preceding gene: 167625069

Following gene: 167625066

Centisome position: 73.71

GC content: 46.03

Gene sequence:

>630_bases
ATGTCAGGAAAAAAACGAACAGCCAGTTCTTCTCGCTGGATGCAGGAACATTTCGATGATCACTATGTCAAACTAGCGCA
GAAGCGCGGTTTAAGGTCGCGTGCGGCTTTTAAGATTGAAGAGATCCAAGAAAAAGATAAACTCATTCGTCCGGGGATGA
CTGTTGTAGATTTAGGGGCTGCACCTGGTGGTTGGTCTCAGGTAGCCGTTAAATTGGCAGGAGACAAGGGTAAAGTTATC
GCTTGTGATATTTTACCGATGGATCCGATTGTTGGCGTTGATTTCCTACAGGGTGATTTTCGCGAAGAAAAAGTACTGGA
CGCATTGTTGACCCGAGTGGGCGATGCCAAGGTTGATGTGGTGCTATCTGATATGGCACCGAATATGAGTGGTACAGGTG
GTGTTGATCAGCCTCGAGCCATGTATTTAGTAGAACTTGCGTTAGATATGTGTCATCAAGTATTGGCACCTAACGGTTGT
TTTGCTGTTAAAGTCTTTCAGGGGGAGGGCTTTGATGAATACATGAAAGCGGTTAAAGAGGCGTTCAAAACGGTTAAAAC
ACGTAAGCCAGATTCTTCACGTCCACGTTCGCGTGAAGTCTATCTTGTGGCGACCGGGTACAAGTTGTAG

Upstream 100 bases:

>100_bases
AGTTCATTTATACCAACTTTATGCTGAGTTACTGTTGAAAACCCGCTATTCTACCCTTATATAGGCTTTGTGTAACTCTC
AATTTTGTTGCGGATTTTAA

Downstream 100 bases:

>100_bases
TAATCTACCGTAAGTACTCTAACATTAGTTTTCGAAAGACTTTAAAAGAGGTCGAGTAATTTGAGTGACATGGCAAAAAA
TTTAATTCTCTGGGTTGTCA

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSGKKRTASSSRWMQEHFDDHYVKLAQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGAAPGGWSQVAVKLAGDKGKVI
ACDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDVVLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGC
FAVKVFQGEGFDEYMKAVKEAFKTVKTRKPDSSRPRSREVYLVATGYKL

Sequences:

>Translated_209_residues
MSGKKRTASSSRWMQEHFDDHYVKLAQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGAAPGGWSQVAVKLAGDKGKVI
ACDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDVVLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGC
FAVKVFQGEGFDEYMKAVKEAFKTVKTRKPDSSRPRSREVYLVATGYKL
>Mature_208_residues
SGKKRTASSSRWMQEHFDDHYVKLAQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGAAPGGWSQVAVKLAGDKGKVIA
CDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDVVLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGCF
AVKVFQGEGFDEYMKAVKEAFKTVKTRKPDSSRPRSREVYLVATGYKL

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=213, Percent_Identity=35.6807511737089, Blast_Score=140, Evalue=7e-34,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=33.5106382978723, Blast_Score=117, Evalue=7e-27,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=34.7368421052632, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=34.7368421052632, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=34.7368421052632, Blast_Score=113, Evalue=1e-25,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=74.0384615384615, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=34.0909090909091, Blast_Score=119, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=32.9842931937173, Blast_Score=106, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=33.6683417085427, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=36.1256544502618, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=282, Percent_Identity=26.5957446808511, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=36.7021276595745, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356387, Length=221, Percent_Identity=34.841628959276, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647580, Length=200, Percent_Identity=32.5, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648639, Length=198, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23082; Mature: 22951

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKKRTASSSRWMQEHFDDHYVKLAQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
APGGWSQVAVKLAGDKGKVIACDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDV
CCCCHHHEEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEE
VLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGCFAVKVFQGEGFDEYMKAVKE
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
AFKTVKTRKPDSSRPRSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
SGKKRTASSSRWMQEHFDDHYVKLAQKRGLRSRAAFKIEEIQEKDKLIRPGMTVVDLGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
APGGWSQVAVKLAGDKGKVIACDILPMDPIVGVDFLQGDFREEKVLDALLTRVGDAKVDV
CCCCHHHEEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEE
VLSDMAPNMSGTGGVDQPRAMYLVELALDMCHQVLAPNGCFAVKVFQGEGFDEYMKAVKE
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
AFKTVKTRKPDSSRPRSREVYLVATGYKL
HHHHHHCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA