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The map label for this gene is grpE

Identifier: 167624994

GI number: 167624994

Start: 3758119

End: 3758721

Strand: Reverse

Name: grpE

Synonym: Shal_3081

Alternate gene names: 167624994

Gene position: 3758721-3758119 (Counterclockwise)

Preceding gene: 167625001

Following gene: 167624990

Centisome position: 71.91

GC content: 46.27

Gene sequence:

>603_bases
ATGAGCAACCAAACAAATAAAGCACAAGATAACCAAGTTGAAGAGATCGTCGAAGGCGAATTGCTTAACGAAAACGGTAC
AGAAGCAACAGGTGAAGCCTCTTTAATGGATGAGTTAACTCAAGCCAATTTCCGTGTTGAAGAGTTAGAAAAAGCTTTGC
AAGAAGCTGAAACTAAAGTTGAATCTCAGAAAGACTCAGTGATCCGTGCAGCAGCCGAGGTAGACAACATCCGTCGTCGT
TCAGCGATTGATGTCGAGAAGGCGCACAAATTTGCCTTAGAAAAATTTATCAACGAGTTGCTACCTGTTTTAGATAACAT
GGAGCGCGCTCTGCAAGGTACCGATGCCGAAGCGGAAGCGACAAAAGCCATTTACGAAGGCGTAGAGCTGACGGCAAAGA
GCTTTGTGAGTACGGTTGAGAAGTTTGGTCTGACGCAAGTCGATCCTCTGGGCGATACATTTAACCCTGAGCTACACCAA
GCTATCGGTATGCAGCCAAGTGCTGATTTCCCTGCCAACACTGTAATGATGGTGATGCAGAAGGGTTACACCCTAAATGA
CCGCCTACTTCGCCCTGCGATGGTAATGGTTTCTCAAGGCTAG

Upstream 100 bases:

>100_bases
TATTTATGTTAGATCCAGCTTTAGGCCTTGAAACCTTCAAATTGATCCCCATAATATGCCTAGAGCATGCAGAAACAAAG
CATTTTTAGCTGGAGCAAAA

Downstream 100 bases:

>100_bases
TTTTCTAGCTAAAGCCAAATATGGCTGTTGCTAGCTTAGCGGTTAGCACAAGCCTAAAAAAGGACTGCCTAGGCAGTCCT
TTTTATTTCGCTATTTTTCA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSNQTNKAQDNQVEEIVEGELLNENGTEATGEASLMDELTQANFRVEELEKALQEAETKVESQKDSVIRAAAEVDNIRRR
SAIDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEATKAIYEGVELTAKSFVSTVEKFGLTQVDPLGDTFNPELHQ
AIGMQPSADFPANTVMMVMQKGYTLNDRLLRPAMVMVSQG

Sequences:

>Translated_200_residues
MSNQTNKAQDNQVEEIVEGELLNENGTEATGEASLMDELTQANFRVEELEKALQEAETKVESQKDSVIRAAAEVDNIRRR
SAIDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEATKAIYEGVELTAKSFVSTVEKFGLTQVDPLGDTFNPELHQ
AIGMQPSADFPANTVMMVMQKGYTLNDRLLRPAMVMVSQG
>Mature_199_residues
SNQTNKAQDNQVEEIVEGELLNENGTEATGEASLMDELTQANFRVEELEKALQEAETKVESQKDSVIRAAAEVDNIRRRS
AIDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEATKAIYEGVELTAKSFVSTVEKFGLTQVDPLGDTFNPELHQA
IGMQPSADFPANTVMMVMQKGYTLNDRLLRPAMVMVSQG

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=157, Percent_Identity=34.3949044585987, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI40255109, Length=160, Percent_Identity=31.25, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1788967, Length=156, Percent_Identity=47.4358974358974, Blast_Score=147, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17552458, Length=141, Percent_Identity=35.4609929078014, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324806, Length=169, Percent_Identity=36.6863905325444, Blast_Score=106, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653432, Length=178, Percent_Identity=29.7752808988764, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_SHEHH (B0TQ37)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675288.1
- ProteinModelPortal:   B0TQ37
- SMR:   B0TQ37
- GeneID:   5904324
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3081
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14150
- BioCyc:   SHAL458817:SHAL_3081-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22120; Mature: 21989

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQTNKAQDNQVEEIVEGELLNENGTEATGEASLMDELTQANFRVEELEKALQEAETKV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ESQKDSVIRAAAEVDNIRRRSAIDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TKAIYEGVELTAKSFVSTVEKFGLTQVDPLGDTFNPELHQAIGMQPSADFPANTVMMVMQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHH
KGYTLNDRLLRPAMVMVSQG
CCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNQTNKAQDNQVEEIVEGELLNENGTEATGEASLMDELTQANFRVEELEKALQEAETKV
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ESQKDSVIRAAAEVDNIRRRSAIDVEKAHKFALEKFINELLPVLDNMERALQGTDAEAEA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TKAIYEGVELTAKSFVSTVEKFGLTQVDPLGDTFNPELHQAIGMQPSADFPANTVMMVMQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHH
KGYTLNDRLLRPAMVMVSQG
CCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA