Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 167624789

GI number: 167624789

Start: 3495355

End: 3496164

Strand: Reverse

Name: paaF [C]

Synonym: Shal_2875

Alternate gene names: 167624789

Gene position: 3496164-3495355 (Counterclockwise)

Preceding gene: 167624790

Following gene: 167624788

Centisome position: 66.89

GC content: 50.49

Gene sequence:

>810_bases
ATGTCTCAGGAGAATGCCAGCACGTTAGCGACAGAGAAATCGCTATTAATCGAACAGATTGTTGGTCACACCGCAGTGAT
CAGCATGAACAATCCGCCAGCCAATACTTGGACTGCCCAGAGCCTGCAGGAGCTAAAGCAAAAAGTACTAGCCCTCAATG
AAAACAAAGAGGTTTACGCCTTGGTGATCACAGCTGAAGGCGATAAGTTTTTCTCGGCGGGTGCAGATCTAAAATTGTTT
GCCGACGGTGATAAGCAAAATGCCGCCGATATGGCGCGATATTTTGGTGAAGCCTTTGAAACATTAAGTACCTTTCACGG
CGTCTCTATCGCGGCGATTAATGGTTACGCCATGGGCGGCGGCTTAGAAGTTGCGCTAGCTTGTGATTTGCGTATTTGTG
AAGAGCAGGCGCAAATGGCACTGCCGGAGGCAACCGTTGGCCTGCTGCCCTGTGCGGGCGGCACCCAAAACTTAACCGCG
CTGGTGGGCGAAGGCTGGGCTAAACGCATGATTTTATGTGGTGAGCGTATTAATGCAGAAAAGGCCGAGAAGATTGGCCT
AGTCGAAGAGGTGGTTGTACAGGGAAATGCCTTTAATGCCGCCATGGCGCTGGCTGAAAAAGTGGCGAACCAGAGTCCAA
GCAGTGTTAAGGCGTGTAAGCAGCTGGTTCAAGCTGGACGCAGCATGCCAAGATCTCAGGTGCTGCCATTAGAGAGAGAG
TATTTTGCTAACCTTTTCGATACCGAAGATCAGGCTGAAGGGGTAAGTGCCTTCTTAAACAAGCGCAAAGCGAACTGGAA
GAACGGCTAA

Upstream 100 bases:

>100_bases
ATTATCTCTCGCCGTTTATTGGATGAAAACGCGGGCGCGATTCTCTAGGCCGCCTAGCATCAGCTTTAAATTGATTTCAG
TTTTATAAAAGGAGTTATCT

Downstream 100 bases:

>100_bases
TGAGGGGAGCTAACTTGGATGGTGCAGCTATGAACGGTGCAGTTATAGGTAGTGAGCTCGAGCTGCAACAAGTGTTGTTT
AGCACACTGGCAACCGACTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSQENASTLATEKSLLIEQIVGHTAVISMNNPPANTWTAQSLQELKQKVLALNENKEVYALVITAEGDKFFSAGADLKLF
ADGDKQNAADMARYFGEAFETLSTFHGVSIAAINGYAMGGGLEVALACDLRICEEQAQMALPEATVGLLPCAGGTQNLTA
LVGEGWAKRMILCGERINAEKAEKIGLVEEVVVQGNAFNAAMALAEKVANQSPSSVKACKQLVQAGRSMPRSQVLPLERE
YFANLFDTEDQAEGVSAFLNKRKANWKNG

Sequences:

>Translated_269_residues
MSQENASTLATEKSLLIEQIVGHTAVISMNNPPANTWTAQSLQELKQKVLALNENKEVYALVITAEGDKFFSAGADLKLF
ADGDKQNAADMARYFGEAFETLSTFHGVSIAAINGYAMGGGLEVALACDLRICEEQAQMALPEATVGLLPCAGGTQNLTA
LVGEGWAKRMILCGERINAEKAEKIGLVEEVVVQGNAFNAAMALAEKVANQSPSSVKACKQLVQAGRSMPRSQVLPLERE
YFANLFDTEDQAEGVSAFLNKRKANWKNG
>Mature_268_residues
SQENASTLATEKSLLIEQIVGHTAVISMNNPPANTWTAQSLQELKQKVLALNENKEVYALVITAEGDKFFSAGADLKLFA
DGDKQNAADMARYFGEAFETLSTFHGVSIAAINGYAMGGGLEVALACDLRICEEQAQMALPEATVGLLPCAGGTQNLTAL
VGEGWAKRMILCGERINAEKAEKIGLVEEVVVQGNAFNAAMALAEKVANQSPSSVKACKQLVQAGRSMPRSQVLPLEREY
FANLFDTEDQAEGVSAFLNKRKANWKNG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=38.5245901639344, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI68989263, Length=206, Percent_Identity=35.4368932038835, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4502327, Length=238, Percent_Identity=30.672268907563, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=33.1460674157303, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=28.2945736434109, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI261878539, Length=113, Percent_Identity=41.5929203539823, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI157694516, Length=227, Percent_Identity=23.3480176211454, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI213417737, Length=227, Percent_Identity=23.3480176211454, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI150378541, Length=249, Percent_Identity=29.3172690763052, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI157694520, Length=207, Percent_Identity=23.6714975845411, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI37594471, Length=212, Percent_Identity=26.4150943396226, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=26.4150943396226, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI70995211, Length=277, Percent_Identity=25.2707581227437, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI62530384, Length=195, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=243, Percent_Identity=37.8600823045267, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=34.3873517786561, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788682, Length=240, Percent_Identity=34.5833333333333, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1790281, Length=126, Percent_Identity=35.7142857142857, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082183, Length=238, Percent_Identity=24.3697478991597, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=26.9230769230769, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=22.1789883268482, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=34.7826086956522, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=35.2226720647773, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17540714, Length=235, Percent_Identity=29.7872340425532, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=169, Percent_Identity=33.7278106508876, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=176, Percent_Identity=34.0909090909091, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=176, Percent_Identity=34.0909090909091, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=176, Percent_Identity=34.0909090909091, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=259, Percent_Identity=22.007722007722, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=164, Percent_Identity=32.3170731707317, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=195, Percent_Identity=27.6923076923077, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=195, Percent_Identity=27.6923076923077, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=267, Percent_Identity=24.7191011235955, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=214, Percent_Identity=24.7663551401869, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=26.2948207171315, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=133, Percent_Identity=29.3233082706767, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=35.2226720647773, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=35.2226720647773, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=29.5019157088123, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24650670, Length=202, Percent_Identity=30.1980198019802, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19922422, Length=243, Percent_Identity=21.8106995884774, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357171, Length=137, Percent_Identity=33.5766423357664, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583165, Length=171, Percent_Identity=28.6549707602339, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19920382, Length=201, Percent_Identity=26.865671641791, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=26.7379679144385, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19921018, Length=170, Percent_Identity=25.8823529411765, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921000, Length=182, Percent_Identity=30.2197802197802, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24583077, Length=182, Percent_Identity=30.2197802197802, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24583079, Length=182, Percent_Identity=30.2197802197802, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28847; Mature: 28716

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQENASTLATEKSLLIEQIVGHTAVISMNNPPANTWTAQSLQELKQKVLALNENKEVYA
CCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
LVITAEGDKFFSAGADLKLFADGDKQNAADMARYFGEAFETLSTFHGVSIAAINGYAMGG
EEEEECCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECC
GLEVALACDLRICEEQAQMALPEATVGLLPCAGGTQNLTALVGEGWAKRMILCGERINAE
CEEEEEEECHHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHCHHHHHHHHHCCCCCHH
KAEKIGLVEEVVVQGNAFNAAMALAEKVANQSPSSVKACKQLVQAGRSMPRSQVLPLERE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHCCCCCHH
YFANLFDTEDQAEGVSAFLNKRKANWKNG
HHHHHCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQENASTLATEKSLLIEQIVGHTAVISMNNPPANTWTAQSLQELKQKVLALNENKEVYA
CCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
LVITAEGDKFFSAGADLKLFADGDKQNAADMARYFGEAFETLSTFHGVSIAAINGYAMGG
EEEEECCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECC
GLEVALACDLRICEEQAQMALPEATVGLLPCAGGTQNLTALVGEGWAKRMILCGERINAE
CEEEEEEECHHHHHHHHHHCCCCHHHEEEECCCCCCHHHHHHHCHHHHHHHHHCCCCCHH
KAEKIGLVEEVVVQGNAFNAAMALAEKVANQSPSSVKACKQLVQAGRSMPRSQVLPLERE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHCCCCCHH
YFANLFDTEDQAEGVSAFLNKRKANWKNG
HHHHHCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA