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The map label for this gene is paaF [C]

Identifier: 167624788

GI number: 167624788

Start: 3494153

End: 3495355

Strand: Reverse

Name: paaF [C]

Synonym: Shal_2874

Alternate gene names: 167624788

Gene position: 3495355-3494153 (Counterclockwise)

Preceding gene: 167624789

Following gene: 167624787

Centisome position: 66.87

GC content: 49.54

Gene sequence:

>1203_bases
ATGAGGGGAGCTAACTTGGATGGTGCAGCTATGAACGGTGCAGTTATAGGTAGTGAGCTCGAGCTGCAACAAGTGTTGTT
TAGCACACTGGCAACCGACTCTGGTAAGCAAATTGGGGTAGCGACTCTTAACATCGAAAAGGCCCTCAATGCGCTGAACC
TTAATATGGTGCAGGCGTTAACCGCTCAGCTCACTGCGTGGCGTCATGATGACAATATCGTTGCCGTGGTGCTCGATGGC
GCGGGTGAAAAAGCCTTTTGTGCCGGTGGTGATGTGCGCGCTATTTATCAAGCATCGGTGCAAACACCGGGCGAGGTAAC
CGCTGCGGCAACCGAGTTTTTTAAGCAGGAGTACGAGCTAGACTATCTACTGCATAGCTTTGGTAAACCCGTCGTTGTTT
GGGGCGATGGCATCGTCATGGGGGGCGGACTAGGTTTGATGATAGGTGCGAGCCACCGCATCGTCACCGAGCGTTCGCGT
ATCGCCATGCCAGAGGTGACGATTGGTTTATATCCTGATGTGGGCGGCAGTTATTTCCTCAATCGCATGCCTGGTAAAAG
TGGTCTATTTTTGGGGATGACAGCCTATAACATGAGTGGCGCCGATGCATTATATGTGGGCATAGGTAACCACTACCTTA
ACGTTGATGATAAAAGCGCACTGTTTGATCAACTTGCGATGTTGCCATGGAGCGACAATTCTGATGACAATCATCATGTG
TTAAATGGCGCTCTTAGCGACATGAGCGCGGCTTGTAAAACGCCGATGGCTGCGAGTCAGCTTAAGCAAAACCAGTTATT
AATTGATGAGTTGATGATAGGAACGCTCGATGAGATCATCTCTCGTGTAAGCTGTTTAGATGAGCAAATCGGTGCGCAGC
ATCCTTGGTTAGCGAAAGCGATTAAGACTCTATTAGCAGGCAGTCCAATCAGCTTAAGTCTGGTGCATCTGCAATCAAAG
TTGAGTGAAGTGCTGGGTAGAGAACCGAGCTTAGCTGAGTGTTTTAAGTTAGAGCTTGGCTTAAGCGTTAATTGCTGCGC
CAAGGCGGATTTTGCCGAAGGAGTACGGGCGCTGCTTATCGATAAAGACCGTAATCCACAGTGGCGCTTTACTGATGTTG
CATCGATCCCACCGGGCTATGCACAAGACTTGCTGCAATCGCCTTGGGATGATGAAAGTCATCCGCTTAAGGCTATGCAC
TAG

Upstream 100 bases:

>100_bases
TAGAGAGAGAGTATTTTGCTAACCTTTTCGATACCGAAGATCAGGCTGAAGGGGTAAGTGCCTTCTTAAACAAGCGCAAA
GCGAACTGGAAGAACGGCTA

Downstream 100 bases:

>100_bases
CTGGTGCATTAAAAGAAACGAGCTAACGGTTAGCCGAGGCGATAGCTTTTTTAAATTAGCTTATTAACAAGAGCGAGTTC
ATCAGCTTTAACAGAAGATA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRGANLDGAAMNGAVIGSELELQQVLFSTLATDSGKQIGVATLNIEKALNALNLNMVQALTAQLTAWRHDDNIVAVVLDG
AGEKAFCAGGDVRAIYQASVQTPGEVTAAATEFFKQEYELDYLLHSFGKPVVVWGDGIVMGGGLGLMIGASHRIVTERSR
IAMPEVTIGLYPDVGGSYFLNRMPGKSGLFLGMTAYNMSGADALYVGIGNHYLNVDDKSALFDQLAMLPWSDNSDDNHHV
LNGALSDMSAACKTPMAASQLKQNQLLIDELMIGTLDEIISRVSCLDEQIGAQHPWLAKAIKTLLAGSPISLSLVHLQSK
LSEVLGREPSLAECFKLELGLSVNCCAKADFAEGVRALLIDKDRNPQWRFTDVASIPPGYAQDLLQSPWDDESHPLKAMH

Sequences:

>Translated_400_residues
MRGANLDGAAMNGAVIGSELELQQVLFSTLATDSGKQIGVATLNIEKALNALNLNMVQALTAQLTAWRHDDNIVAVVLDG
AGEKAFCAGGDVRAIYQASVQTPGEVTAAATEFFKQEYELDYLLHSFGKPVVVWGDGIVMGGGLGLMIGASHRIVTERSR
IAMPEVTIGLYPDVGGSYFLNRMPGKSGLFLGMTAYNMSGADALYVGIGNHYLNVDDKSALFDQLAMLPWSDNSDDNHHV
LNGALSDMSAACKTPMAASQLKQNQLLIDELMIGTLDEIISRVSCLDEQIGAQHPWLAKAIKTLLAGSPISLSLVHLQSK
LSEVLGREPSLAECFKLELGLSVNCCAKADFAEGVRALLIDKDRNPQWRFTDVASIPPGYAQDLLQSPWDDESHPLKAMH
>Mature_400_residues
MRGANLDGAAMNGAVIGSELELQQVLFSTLATDSGKQIGVATLNIEKALNALNLNMVQALTAQLTAWRHDDNIVAVVLDG
AGEKAFCAGGDVRAIYQASVQTPGEVTAAATEFFKQEYELDYLLHSFGKPVVVWGDGIVMGGGLGLMIGASHRIVTERSR
IAMPEVTIGLYPDVGGSYFLNRMPGKSGLFLGMTAYNMSGADALYVGIGNHYLNVDDKSALFDQLAMLPWSDNSDDNHHV
LNGALSDMSAACKTPMAASQLKQNQLLIDELMIGTLDEIISRVSCLDEQIGAQHPWLAKAIKTLLAGSPISLSLVHLQSK
LSEVLGREPSLAECFKLELGLSVNCCAKADFAEGVRALLIDKDRNPQWRFTDVASIPPGYAQDLLQSPWDDESHPLKAMH

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=343, Percent_Identity=37.0262390670554, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI37594469, Length=312, Percent_Identity=35.8974358974359, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI194097323, Length=190, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1787659, Length=161, Percent_Identity=27.9503105590062, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI25144160, Length=371, Percent_Identity=36.1185983827493, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI25144157, Length=371, Percent_Identity=36.1185983827493, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17540714, Length=186, Percent_Identity=26.3440860215054, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=378, Percent_Identity=29.3650793650794, Blast_Score=146, Evalue=7e-36,
Organism=Drosophila melanogaster, GI28571729, Length=373, Percent_Identity=36.4611260053619, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI28571730, Length=373, Percent_Identity=36.4611260053619, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI20129971, Length=188, Percent_Identity=30.8510638297872, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653477, Length=188, Percent_Identity=30.8510638297872, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653139, Length=197, Percent_Identity=28.4263959390863, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 42987; Mature: 42987

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGANLDGAAMNGAVIGSELELQQVLFSTLATDSGKQIGVATLNIEKALNALNLNMVQAL
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHH
TAQLTAWRHDDNIVAVVLDGAGEKAFCAGGDVRAIYQASVQTPGEVTAAATEFFKQEYEL
HHHHHHEECCCCEEEEEEECCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
DYLLHSFGKPVVVWGDGIVMGGGLGLMIGASHRIVTERSRIAMPEVTIGLYPDVGGSYFL
HHHHHHCCCCEEEECCCEEECCCEEEEEECCCEEEECCCCCCCCCEEEEECCCCCCHHHH
NRMPGKSGLFLGMTAYNMSGADALYVGIGNHYLNVDDKSALFDQLAMLPWSDNSDDNHHV
HCCCCCCCEEEEEEEECCCCCCEEEEECCCEEECCCCHHHHHHHHHCCCCCCCCCCCCHH
LNGALSDMSAACKTPMAASQLKQNQLLIDELMIGTLDEIISRVSCLDEQIGAQHPWLAKA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IKTLLAGSPISLSLVHLQSKLSEVLGREPSLAECFKLELGLSVNCCAKADFAEGVRALLI
HHHHHCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHCCEEEEE
DKDRNPQWRFTDVASIPPGYAQDLLQSPWDDESHPLKAMH
ECCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRGANLDGAAMNGAVIGSELELQQVLFSTLATDSGKQIGVATLNIEKALNALNLNMVQAL
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHH
TAQLTAWRHDDNIVAVVLDGAGEKAFCAGGDVRAIYQASVQTPGEVTAAATEFFKQEYEL
HHHHHHEECCCCEEEEEEECCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
DYLLHSFGKPVVVWGDGIVMGGGLGLMIGASHRIVTERSRIAMPEVTIGLYPDVGGSYFL
HHHHHHCCCCEEEECCCEEECCCEEEEEECCCEEEECCCCCCCCCEEEEECCCCCCHHHH
NRMPGKSGLFLGMTAYNMSGADALYVGIGNHYLNVDDKSALFDQLAMLPWSDNSDDNHHV
HCCCCCCCEEEEEEEECCCCCCEEEEECCCEEECCCCHHHHHHHHHCCCCCCCCCCCCHH
LNGALSDMSAACKTPMAASQLKQNQLLIDELMIGTLDEIISRVSCLDEQIGAQHPWLAKA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IKTLLAGSPISLSLVHLQSKLSEVLGREPSLAECFKLELGLSVNCCAKADFAEGVRALLI
HHHHHCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCHHHCCEEEEE
DKDRNPQWRFTDVASIPPGYAQDLLQSPWDDESHPLKAMH
ECCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA