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The map label for this gene is purF [H]

Identifier: 167624557

GI number: 167624557

Start: 3198108

End: 3199622

Strand: Reverse

Name: purF [H]

Synonym: Shal_2637

Alternate gene names: 167624557

Gene position: 3199622-3198108 (Counterclockwise)

Preceding gene: 167624558

Following gene: 167624556

Centisome position: 61.21

GC content: 46.4

Gene sequence:

>1515_bases
ATGTGTGGTATTGTCGGAATAGTTGGCCAGTCATCGGTTAATCAAACCATTTATGATGCACTGACTGTGCTCCAGCATCG
TGGACAGGATGCTGCTGGCATTGTGACCGTCGATGGCAGTGCGTTTAGGCTGCGTAAAGCCAATGGTTTGGTGAAAGACG
TTTTTGAGCCAAAGCATATGCAGCGCCTACAAGGTAATGCTGGCATAGGTCACGTGCGTTACCCTACTGCAGGAAGCTCT
AGTGCTTCTGAAGCTCAGCCATTTTATGTTAACTCTCCTTTCGGTATTTCATTAGCGCACAATGGTAACTTAACCAATAC
GGTTGAGCTGCATGAGCGTTTAGTGAAGCGGCGCCGTCATGTTAATACGACTTCAGACTCGGAAGTGCTACTTAACTTAC
TCGCCGATGAACTGCAACAGAGCACATCAGATGATTTGTCAGCAGATGATGTGTTTAACGCAGTGGTTAAGGTACACGAT
ATTACTCGTGGCGCTTATGCGGTTGCAGCCATGATTATTGGTCAAGGCTTAGTTGCATTTAGAGATCCCTTTGGTATTCG
TCCGCTAGTGCTAGGTAAGCATGAAACGGCAACGGGCACCGAGTATATGATTGCATCGGAAAGTGTCGCCCTAGATGCAG
TTGGTTTTGAGGTTATGCGCGATGTCGCGCCGGGTGAAGCGGTTTATATCACCCTCGACGGTGAACTTTATACACGCCAA
TGTGCTCACTCGCCAAGCTACTCTCCTTGTATTTTCGAATTCGTTTATTTTGCTCGTCCAGACTCGACGATTGATAACAT
CTCTGTTTATAGCAGTCGCGTCAATATGGGAACCATGCTGGGTGAAAAGATTAAGAGAGAGTGGGAAGATCATGATATCG
ATGTCGTGATCCCTATTCCTGAAACCTCATGTGATATCGCGCTCGAGATCGCGCGTCACATGGATCTACCTTATCGCCAA
GGTTTCGTTAAGAACCGATATATCGGTCGTACCTTTATCATGCCGGGCCAGCAAGAGCGCAAGAAGTCAGTGCGCCGTAA
GCTAAATGCGATTAAAGCCGAATTTGAAGGTAAGAACGTGTTACTGGTTGATGACTCCATTGTTCGTGGTACGACTTCTG
AGCAGATCATCGAGATGGCACGTGACGCCGGTGCCAAGAAAGTATATTTCGCGTCAGCAGCACCTGAAATCCGCTTCCCG
AATGTGTACGGTATCGATATGCCAACCACTAACGAATTGATTGCTCATGGTCGAGATGTTGAAGAGATCAATGCGTTGAT
TGGTGCCGACGGCATGATTTTCCAATCACTGCCGGATTTAATTGAAGCGGTACGTAAAGAAAATCCAGCGATTAAGCGTT
TTGAAACCTCAGTATTTGACGGTAAATATGTCACCAATGACGTGAATCAAGAGTACTTAGATTACATCACGCAGTTGCGT
AACGATGATGCCAAAGCCAATCGTAGCAAAGATATTGGCACTAACTTAGAGATGCATAACGTCTGCCATCCTTAA

Upstream 100 bases:

>100_bases
AAAATGCACTAAAGCATTACTTGGTTTTAGGTGAGTATTTGTATTCACCTAAAGCAAAAATATTAAATAATAGAACATCT
TACAATGAGGAAGCTTACCC

Downstream 100 bases:

>100_bases
GTTGCTAAAGCCCCATGAAACAAGCCGAGCAATAGCTCGGCTTTTTTAGCGCTAGCATTTGTGTTTAAAGTGCCAAGTGG
GAATGCTTCTTTGCAAAATA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELHERLVKRRRHVNTTSDSEVLLNLLADELQQSTSDDLSADDVFNAVVKVHD
ITRGAYAVAAMIIGQGLVAFRDPFGIRPLVLGKHETATGTEYMIASESVALDAVGFEVMRDVAPGEAVYITLDGELYTRQ
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIPETSCDIALEIARHMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAIKAEFEGKNVLLVDDSIVRGTTSEQIIEMARDAGAKKVYFASAAPEIRFP
NVYGIDMPTTNELIAHGRDVEEINALIGADGMIFQSLPDLIEAVRKENPAIKRFETSVFDGKYVTNDVNQEYLDYITQLR
NDDAKANRSKDIGTNLEMHNVCHP

Sequences:

>Translated_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELHERLVKRRRHVNTTSDSEVLLNLLADELQQSTSDDLSADDVFNAVVKVHD
ITRGAYAVAAMIIGQGLVAFRDPFGIRPLVLGKHETATGTEYMIASESVALDAVGFEVMRDVAPGEAVYITLDGELYTRQ
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIPETSCDIALEIARHMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAIKAEFEGKNVLLVDDSIVRGTTSEQIIEMARDAGAKKVYFASAAPEIRFP
NVYGIDMPTTNELIAHGRDVEEINALIGADGMIFQSLPDLIEAVRKENPAIKRFETSVFDGKYVTNDVNQEYLDYITQLR
NDDAKANRSKDIGTNLEMHNVCHP
>Mature_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELHERLVKRRRHVNTTSDSEVLLNLLADELQQSTSDDLSADDVFNAVVKVHD
ITRGAYAVAAMIIGQGLVAFRDPFGIRPLVLGKHETATGTEYMIASESVALDAVGFEVMRDVAPGEAVYITLDGELYTRQ
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIPETSCDIALEIARHMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAIKAEFEGKNVLLVDDSIVRGTTSEQIIEMARDAGAKKVYFASAAPEIRFP
NVYGIDMPTTNELIAHGRDVEEINALIGADGMIFQSLPDLIEAVRKENPAIKRFETSVFDGKYVTNDVNQEYLDYITQLR
NDDAKANRSKDIGTNLEMHNVCHP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=35.7894736842105, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1788651, Length=503, Percent_Identity=73.558648111332, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=173, Percent_Identity=32.9479768786127, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=32.6446280991736, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6323958, Length=508, Percent_Identity=50.5905511811024, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24659598, Length=493, Percent_Identity=34.2799188640974, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24659604, Length=469, Percent_Identity=34.9680170575693, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI28573187, Length=472, Percent_Identity=34.1101694915254, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55803; Mature: 55803

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHCCHHHHHCCHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELHERLVKRRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQQSTSDDLSADDVFNAVVKVHDITRGAYAVAAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEE
RDPFGIRPLVLGKHETATGTEYMIASESVALDAVGFEVMRDVAPGEAVYITLDGELYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARHMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAIKAEFEGKNV
CCCHHHHHHHHHHCCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEE
LLVDDSIVRGTTSEQIIEMARDAGAKKVYFASAAPEIRFPNVYGIDMPTTNELIAHGRDV
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHCCCCH
EEINALIGADGMIFQSLPDLIEAVRKENPAIKRFETSVFDGKYVTNDVNQEYLDYITQLR
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHC
NDDAKANRSKDIGTNLEMHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDGSAFRLRKANGLVKDVFEPKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHCCHHHHHCCHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELHERLVKRRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQQSTSDDLSADDVFNAVVKVHDITRGAYAVAAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEE
RDPFGIRPLVLGKHETATGTEYMIASESVALDAVGFEVMRDVAPGEAVYITLDGELYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
CAHSPSYSPCIFEFVYFARPDSTIDNISVYSSRVNMGTMLGEKIKREWEDHDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARHMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAIKAEFEGKNV
CCCHHHHHHHHHHCCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEE
LLVDDSIVRGTTSEQIIEMARDAGAKKVYFASAAPEIRFPNVYGIDMPTTNELIAHGRDV
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHCCCCH
EEINALIGADGMIFQSLPDLIEAVRKENPAIKRFETSVFDGKYVTNDVNQEYLDYITQLR
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHC
NDDAKANRSKDIGTNLEMHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]